FastQCFastQC Report
Wed 25 May 2016
SRR1780397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91973
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT3065933.33478303415133No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT2621928.507279310232352RNA PCR Primer, Index 24 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC75748.235025496613137No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG44584.847074684961891No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC41674.530677481434768No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT24702.6855707653333045No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC12001.304730736194318No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC11401.239494199384602No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG9281.008991769323606No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC8460.919835169016994No Hit
CTTAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC7260.7893620953975623No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC6980.7589183782196949No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG6700.7284746610418275No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG4240.4610048601219923No Hit
CTTATTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG3340.3631500549074185No Hit
CTTATCACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC3060.33270633772955105No Hit
CTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT3020.32835723527557No Hit
TCTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT2930.31857175475411265No Hit
ACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTTCT2630.28595348634925466RNA PCR Primer, Index 24 (95% over 23bp)
ATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTTCTGCTT2050.2228915007665293Illumina PCR Primer Index 6 (96% over 26bp)
TCTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT2030.22071694953953877No Hit
CTTATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC1770.1924477835886619No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT1660.18048775184021396Illumina PCR Primer Index 2 (95% over 21bp)
TACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTTC990.10764028573603122Illumina PCR Primer Index 6 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCA207.76406E-444.0626341
TCTTTAC254.3739252E-544.0626341
TCTTAAT800.044.0626341
CCGTCTT28900.043.2531944
CTTTACA8950.042.8318371
TTTACAC8950.042.785232
TCTTTAT850.041.470721
CTTATAA750.041.1251261
TATAACA700.040.8707853
TTATAAC700.040.8707852
CTTACAC603.6379788E-1240.390751
GCCGTCT32000.039.06303843
TTACACA9900.038.6795773
CTTAACA800.038.5548061
TTAACAC800.038.512852
CTATACA4250.038.3604131
ATAACAC750.038.1460654
CTTATAC38000.037.395261
CTCTTAT1700.036.2868771
TTATACA40200.035.3102952
Produced by FastQC (version 0.11.5)