FastQCFastQC Report
Wed 25 May 2016
SRR1780396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48165
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAA150.002212856769.9927420
CCTGACC150.002212856769.992748
AGACTGG200.006914695352.4945535
TTTAAAG200.006914695352.4945526
TTTGGAG200.006914695352.4945531
GGGCCTA200.006914695352.4945555
TTAAAGG200.006914695352.4945527
AGTAGTG200.006914695352.494555
GAAGGGC200.006914695352.4945536
AGAAGCC400.002397784734.99636569
TATCCAT400.002397784734.99636542
CCGACGA2051.2111179E-515.36425918
GCCGACG2153.139336E-413.02190317
TGCCGAC2500.001116464411.19883716
GCTGCCG3004.2788553E-410.4989114
ACGCTGC3100.00664828359.03132112
CGCTGCC3200.0086263278.74909113
Produced by FastQC (version 0.11.5)