##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780396_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48165 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.281760614554138 33.0 21.0 34.0 12.0 34.0 2 28.3147721374442 34.0 23.0 34.0 11.0 34.0 3 28.55081490709021 34.0 23.0 34.0 11.0 34.0 4 29.086141388975395 34.0 23.0 34.0 11.0 34.0 5 28.89861932938856 34.0 23.0 34.0 11.0 34.0 6 30.983701858195786 37.0 26.0 38.0 11.0 38.0 7 30.61056783971764 36.0 26.0 38.0 11.0 38.0 8 29.859399979238034 36.0 24.0 38.0 11.0 38.0 9 30.69139416588809 36.0 26.0 38.0 12.0 38.0 10-11 30.840921831205232 37.0 26.0 38.0 11.0 38.0 12-13 31.100570954012248 37.0 26.0 38.0 11.0 38.0 14-15 30.857614450327002 37.0 26.0 38.0 11.0 38.0 16-17 30.62948198899616 37.0 26.0 38.0 11.0 38.0 18-19 30.690760925983597 37.0 25.5 38.0 11.0 38.0 20-21 30.77123429876466 37.0 26.0 38.0 11.0 38.0 22-23 30.503301152289005 37.0 25.0 38.0 11.0 38.0 24-25 30.92946122703208 37.0 26.0 38.0 11.0 38.0 26-27 30.860791030831514 37.0 26.0 38.0 11.0 38.0 28-29 30.874888404443062 37.0 26.0 38.0 11.0 38.0 30-31 30.799719713484897 37.0 26.0 38.0 11.0 38.0 32-33 30.643247171182395 37.0 25.5 38.0 11.0 38.0 34-35 30.722038824872833 37.0 26.0 38.0 11.0 38.0 36-37 30.683556524447212 37.0 26.0 38.0 11.0 38.0 38-39 30.86128931796948 37.0 26.0 38.0 11.0 38.0 40-41 30.575397072563064 37.0 25.0 38.0 11.0 38.0 42-43 30.815716806809924 37.0 26.0 38.0 11.0 38.0 44-45 30.09504827156649 36.0 24.0 38.0 11.0 38.0 46-47 30.676985362815323 37.0 26.0 38.0 11.0 38.0 48-49 30.609654313298037 37.0 25.5 38.0 11.0 38.0 50-51 30.459493408076405 36.5 25.0 38.0 11.0 38.0 52-53 30.452849579570227 37.0 25.0 38.0 11.0 38.0 54-55 30.312996989515206 36.0 24.0 38.0 11.0 38.0 56-57 30.178885082528808 36.5 24.0 38.0 11.0 38.0 58-59 30.133354095297413 36.5 24.0 38.0 11.0 38.0 60-61 30.208834215716806 36.0 24.0 38.0 11.0 38.0 62-63 30.149496522371017 36.0 24.0 38.0 11.0 38.0 64-65 30.25693968649434 36.5 24.0 38.0 11.0 38.0 66-67 30.258133499429043 36.5 24.0 38.0 11.0 38.0 68-69 29.758123118447006 36.0 23.5 38.0 11.0 38.0 70-71 29.86772552683484 36.0 24.0 38.0 11.0 38.0 72-73 30.07025848645282 36.0 24.0 38.0 11.0 38.0 74-75 29.661040174400497 35.5 23.5 38.0 11.0 38.0 76 29.03986297103706 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.942834855459406 1101 2 -0.6103109996552867 1101 3 -0.9937267346552972 1101 4 -1.6813631641971583 1101 5 -1.775351613549411 1101 6 -2.777188623673773 1101 7 -1.3999819549909596 1101 8 -0.6215534091235746 1101 9 -1.0147976921080861 1101 10-11 -1.3882811910250794 1101 12-13 -1.6534164117576715 1101 14-15 -0.5209106360223856 1101 16-17 -0.9127472448300402 1101 18-19 -0.9609576947680765 1101 20-21 -0.977216145022151 1101 22-23 -0.2058893282239751 1101 24-25 -1.6554917217850829 1101 26-27 -0.6424104767065373 1101 28-29 -0.4240748461098107 1101 30-31 -1.0198750076217813 1101 32-33 -0.27057125828254414 1101 34-35 -0.8038362252998112 1101 36-37 0.1402181354411276 1101 38-39 -0.6024874156915239 1101 40-41 -0.46941625812925736 1101 42-43 -0.04334971530132492 1101 44-45 -0.44799830360947723 1101 46-47 -1.1228613118779123 1101 48-49 -1.9521312903906498 1101 50-51 -0.8728698265606987 1101 52-53 -0.4574272717687613 1101 54-55 -1.5209699473997453 1101 56-57 -0.6410673132010629 1101 58-59 -0.7934500593936953 1101 60-61 -0.1912559859308125 1101 62-63 -1.5994613473519195 1101 64-65 -0.5849315832197135 1101 66-67 -1.2407898267632014 1101 68-69 -0.5907448332796719 1101 70-71 -0.0986119103181835 1101 72-73 -1.273719349631655 1101 74-75 -1.369457815094453 1101 76 -1.9789696151134173 1102 1 0.2006525394985772 1102 2 -1.0277479744452016 1102 3 -0.09897883549563247 1102 4 0.2335528021893971 1102 5 -0.2358558152300816 1102 6 0.5228113763262279 1102 7 1.3792197256813132 1102 8 0.6494549942377681 1102 9 0.1957065095725845 1102 10-11 -0.522314804470458 1102 12-13 -3.0553071680601924 1102 14-15 -1.7582005519887716 1102 16-17 -1.7252472448300402 1102 18-19 -1.0960417283815218 1102 20-21 -0.7631405147700505 1102 22-23 -0.4887674794844763 1102 24-25 0.5621553370384476 1102 26-27 -3.9156247624208227 1102 28-29 -0.007373165437542184 1102 30-31 0.6468266730504872 1102 32-33 -5.2663695776102735 1102 34-35 -0.8511051328628376 1102 36-37 -0.0037944696008871404 1102 38-39 -0.017928592162110846 1102 40-41 0.24182323766906322 1102 42-43 -0.23620685815846798 1102 44-45 -5.061443681760736 1102 46-47 -0.31981509339051684 1102 48-49 -0.07639599627300342 1102 50-51 -5.422974868577505 1102 52-53 -1.6850533221889314 1102 54-55 0.01674013663386731 1102 56-57 -0.3836093300077863 1102 58-59 1.2109617053121902 1102 60-61 0.9373154426406138 1102 62-63 -2.70881008684772 1102 64-65 -0.924322339522238 1102 66-67 0.4678236186149505 1102 68-69 -5.6448414719351305 1102 70-71 -0.2892631708223874 1102 72-73 0.4300621629733854 1102 74-75 -4.624709915934787 1102 76 -1.1764486067100535 1103 1 -0.2870551016974332 1103 2 -0.20897721032559957 1103 3 -0.18917817104380674 1103 4 0.486044496541556 1103 5 0.013978071813106396 1103 6 -1.5039328097202862 1103 7 -0.3953649919266624 1103 8 0.235833731779298 1103 9 -1.2401738890985108 1103 10-11 -0.3730623127761028 1103 12-13 -0.9783138125784632 1103 14-15 -0.49175536926451713 1103 16-17 -0.8240844541323646 1103 18-19 -1.5348291037968025 1103 20-21 -1.1473597838730392 1103 22-23 -0.6996478781555737 1103 24-25 -0.2645888490080637 1103 26-27 -0.8444453604274678 1103 28-29 -0.2813764876966758 1103 30-31 -0.42368827711562673 1103 32-33 -4.358977551032201 1103 34-35 -0.8913875248894172 1103 36-37 -0.5891765294015521 1103 38-39 0.31346675667510127 1103 40-41 0.36001260643982747 1103 42-43 -0.16544273855713953 1103 44-45 -4.069749329601269 1103 46-47 -0.17612738574931797 1103 48-49 -0.7083727404590512 1103 50-51 -0.5509649018000964 1103 52-53 -0.5987576411258075 1103 54-55 0.3163248542418451 1103 56-57 0.13159000554038514 1103 58-59 -0.3541545737575795 1103 60-61 0.5413021536040716 1103 62-63 -0.2900393227281164 1103 64-65 -0.18453828636608804 1103 66-67 -0.10501691294651039 1103 68-69 -2.074243465290614 1103 70-71 -0.7655920744768707 1103 72-73 0.37607545200993187 1103 74-75 -2.5474673910178467 1103 76 -1.373292460530653 1104 1 -0.43359654475050746 1104 2 -0.14368204037926802 1104 3 1.0724497359329384 1104 4 0.18684950548610146 1104 5 -0.03402431339858225 1104 6 -0.2643681108532583 1104 7 1.2182307146923002 1104 8 0.7611766059593812 1104 9 0.8143878282539028 1104 10-11 0.3115679794123274 1104 12-13 -0.1298492926023158 1104 14-15 0.4239423051540854 1104 16-17 1.0215476269648285 1104 18-19 0.7437934364536432 1104 20-21 1.4705591189295824 1104 22-23 0.38055486483786893 1104 24-25 0.9653604652435703 1104 26-27 0.8079100361139737 1104 28-29 0.5130480799837045 1104 30-31 0.17045304667686167 1104 32-33 -1.1715893578300545 1104 34-35 0.46162380486610033 1104 36-37 0.18952054871413182 1104 38-39 -0.37369782293134435 1104 40-41 1.0137096845555114 1104 42-43 0.9438297718781641 1104 44-45 -2.1076891030061553 1104 46-47 0.5414486428732204 1104 48-49 1.8139886191116084 1104 50-51 0.9952485746459381 1104 52-53 1.0275840404484313 1104 54-55 -0.015219570325836429 1104 56-57 0.9821415857431326 1104 58-59 0.7263463206968055 1104 60-61 0.0811799115050853 1104 62-63 0.7656404626028319 1104 64-65 0.8472893820894853 1104 66-67 0.772219223010552 1104 68-69 -3.1315630836567436 1104 70-71 -0.24576500232421594 1104 72-73 1.159915642826867 1104 74-75 0.18710327087839573 1104 76 0.6605477302862823 1105 1 -0.5472128135391898 1105 2 0.3339925838470812 1105 3 -1.1430675054463713 1105 4 0.26598301565409344 1105 5 -0.24644694823500757 1105 6 0.803271146441169 1105 7 0.8577090524464985 1105 8 -0.15924779722364235 1105 9 -0.34542649535352155 1105 10-11 0.710772223444156 1105 12-13 -0.8891331122309651 1105 14-15 -0.6392760856505113 1105 16-17 0.2244653988481211 1105 18-19 -0.2593011700892376 1105 20-21 -0.48169552298023177 1105 22-23 -0.014670599353113545 1105 24-25 0.21071855542925277 1105 26-27 0.40428492559231444 1105 28-29 -0.7130792409712043 1105 30-31 0.014396459585793053 1105 32-33 0.41815423191353673 1105 34-35 0.46129223987936285 1105 36-37 0.17161603943031167 1105 38-39 -0.14292859216211085 1105 40-41 -0.38206838794669906 1105 42-43 -0.014614083117415078 1105 44-45 0.8012410473033036 1105 46-47 0.3257103581694203 1105 48-49 -0.5700741571925434 1105 50-51 -0.30614399829836003 1105 52-53 -1.0450286916470581 1105 54-55 -1.5114618338094807 1105 56-57 -0.26509537270072414 1105 58-59 0.050616877725982334 1105 60-61 0.06736470372436187 1105 62-63 -0.33758676993474523 1105 64-65 -0.47366552507231674 1105 66-67 0.6267727154950791 1105 68-69 0.7055279861929478 1105 70-71 0.6854905237588973 1105 72-73 -0.7721135348919645 1105 74-75 -0.8966721491039209 1105 76 -2.586136619846343 1106 1 6.525394985779087E-4 1106 2 0.4793948826976546 1106 3 0.4581640216472245 1106 4 0.32640994504653875 1106 5 0.5570013276270593 1106 6 0.45852566204051115 1106 7 1.1006482971098812 1106 8 1.206597851380625 1106 9 1.8885636524297276 1106 10-11 1.4419709098152573 1106 12-13 1.1768356890826652 1106 14-15 0.9596565908683701 1106 16-17 0.8961813265985334 1106 18-19 0.15752970018990808 1106 20-21 0.2832880566585203 1106 22-23 0.3790896633726675 1106 24-25 0.7728696227527294 1106 26-27 1.816518094722035 1106 28-29 -0.01808745115182475 1106 30-31 -0.017459041235223793 1106 32-33 1.5907732795325842 1106 34-35 0.8453234385657353 1106 36-37 1.0390626732562573 1106 38-39 1.2713571221236002 1106 40-41 -0.04031961947379514 1106 42-43 0.7852217132701043 1106 44-45 1.102842032524979 1106 46-47 1.590899192323775 1106 48-49 1.3271754322984215 1106 50-51 0.9663108457082075 1106 52-53 0.40423239209678385 1106 54-55 0.0560258509195819 1106 56-57 1.5056763842779297 1106 58-59 0.11453313388361508 1106 60-61 1.8194582997834736 1106 62-63 1.1411899131522816 1106 64-65 1.0399633747634773 1106 66-67 0.6142521900435192 1106 68-69 0.9658728137791535 1106 70-71 0.4785939720347585 1106 72-73 0.25506216297338824 1106 74-75 -0.8318527730776459 1106 76 -0.7407343209957702 1107 1 0.15753722108205537 1107 2 -0.10313523778427225 1107 3 0.13570274798113147 1107 4 -0.14123205151111762 1107 5 -0.42613120249342273 1107 6 -0.06634525017979698 1107 7 -0.17310385435311204 1107 8 -0.576018327621096 1107 9 -0.9573915971399778 1107 10-11 -1.1125901917337977 1107 12-13 0.5846239851239758 1107 14-15 -0.4134070924362767 1107 16-17 -0.030970136396305747 1107 18-19 0.19212522514688146 1107 20-21 0.6569971788616193 1107 22-23 -0.3295161886066822 1107 24-25 -0.2381458677808297 1107 26-27 0.14448711365490752 1107 28-29 -0.2491545767972667 1107 30-31 -0.18983425638152696 1107 32-33 0.2605237098251827 1107 34-35 -0.9311395562707574 1107 36-37 -0.22853629404150766 1107 38-39 -0.543531001800666 1107 40-41 0.43149621529384774 1107 42-43 -1.0313015225302422 1107 44-45 -0.20322509312046222 1107 46-47 -0.3325087250600518 1107 48-49 -0.2336249119356566 1107 50-51 -0.7545583453417066 1107 52-53 -0.08698963888428324 1107 54-55 -0.24930977730761228 1107 56-57 0.3006849901299091 1107 58-59 -0.4944599814347974 1107 60-61 -0.8508945401476815 1107 62-63 -0.6435346995843787 1107 64-65 0.4897051992040993 1107 66-67 -0.2073915276845355 1107 68-69 0.16647522341770582 1107 70-71 0.15028071902271023 1107 72-73 0.04688660359300911 1107 74-75 -0.5939439951086278 1107 76 -0.493143959549986 1108 1 -1.0713784415685481 1108 2 -1.386267767904755 1108 3 -0.8281526737056168 1108 4 -0.8580995213906384 1108 5 -0.594805901288602 1108 6 -0.01815247909545903 1108 7 -0.05262192664227072 1108 8 0.20109010611384548 1108 9 -0.28269280195925717 1108 10-11 0.20367486850716787 1108 12-13 -1.3400748100567483 1108 14-15 -0.5429251647254318 1108 16-17 -0.4466327869987161 1108 18-19 -0.2657061001543255 1108 20-21 -0.3821594476444048 1108 22-23 -1.0765041404139097 1108 24-25 -0.7773024942868574 1108 26-27 -1.0512960188752132 1108 28-29 0.01590566416658845 1108 30-31 -0.08140052144176835 1108 32-33 0.35389720380108614 1108 34-35 -0.7293323273550953 1108 36-37 -0.734560390427049 1108 38-39 0.3088786367535512 1108 40-41 -0.08055197747723852 1108 42-43 -0.05539790807240763 1108 44-45 -0.6972009967349173 1108 46-47 -0.3987737853010138 1108 48-49 -0.3601309360320393 1108 50-51 0.17315249803179 1108 52-53 0.06060072256149951 1108 54-55 -0.03545435562086752 1108 56-57 0.19225125519015052 1108 58-59 -1.012532270591425 1108 60-61 0.02862353214147717 1108 62-63 -0.3393178321144994 1108 64-65 -0.49523455983204556 1108 66-67 -0.0025722505761009984 1108 68-69 0.6423788378755404 1108 70-71 0.9996783093841586 1108 72-73 0.24266973612312626 1108 74-75 -0.05177532040983124 1108 76 1.1574584500885692 1109 1 -1.222058083212044 1109 2 -1.855220501917728 1109 3 -0.4019092384260361 1109 4 -0.26827869964210294 1109 5 0.5108474814732133 1109 6 -0.6041117005968495 1109 7 -1.6984359519743677 1109 8 -0.822156727373951 1109 9 -0.7561249922589148 1109 10-11 -0.6083038154594682 1109 12-13 -0.8285672413202647 1109 14-15 -0.6786217974100168 1109 16-17 -0.7156318602146534 1109 18-19 -0.27864246098225465 1109 20-21 -1.0871331887627242 1109 22-23 -0.7572656479826456 1109 24-25 -0.7077164578333495 1109 26-27 -0.9420899638860263 1109 28-29 -0.42284935591372985 1109 30-31 -0.3039059276821128 1109 32-33 0.3412311549904601 1109 34-35 -0.692222348980053 1109 36-37 0.04208465127823402 1109 38-39 -0.41536448959800865 1109 40-41 0.16435071019653336 1109 42-43 0.02395797700636848 1109 44-45 0.062182691865640294 1109 46-47 -0.926500075075495 1109 48-49 -0.9840883039653114 1109 50-51 0.18435113874850018 1109 52-53 0.6173276301920225 1109 54-55 -0.2620144421207087 1109 56-57 0.3026544062559502 1109 58-59 -0.5396793203288368 1109 60-61 0.2734876038127787 1109 62-63 -0.952308255345887 1109 64-65 -0.38668497688487236 1109 66-67 0.035039735831066565 1109 68-69 0.8556164035227454 1109 70-71 -0.7393547459139604 1109 72-73 -0.3048279469167241 1109 74-75 0.20953916831429353 1109 76 -0.4163753466367943 1110 1 -0.011380058775628754 1110 2 -0.8800010904567088 1110 3 -0.684261673463034 1110 4 -0.2599754816073414 1110 5 0.011363743734442266 1110 6 -1.3711483552173966 1110 7 -0.3812788361595345 1110 8 -0.34431298275168487 1110 9 -0.7830566735530127 1110 10-11 -1.4418737689958654 1110 12-13 -0.49308281523181563 1110 14-15 -1.0425006478660492 1110 16-17 -0.3166901978501748 1110 18-19 0.27670515560697595 1110 20-21 -0.3671194217690932 1110 22-23 -0.48749710556309367 1110 24-25 0.3707123840183115 1110 26-27 -1.6143064498608979 1110 28-29 -0.5895160225803977 1110 30-31 -1.4428665196628394 1110 32-33 0.0652459545086117 1110 34-35 0.024973486504372033 1110 36-37 -0.6250552557945603 1110 38-39 -0.3107138270614378 1110 40-41 -0.4527117575370738 1110 42-43 0.40295900953088903 1110 44-45 1.0252054074051316 1110 46-47 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-0.2780823272262509 2116 34-35 -0.9313662695755909 2116 36-37 -0.08301873995419129 2116 38-39 0.08825420353681679 2116 40-41 -0.5827543352956077 2116 42-43 -0.4573282099249809 2116 44-45 0.2870586223867271 2116 46-47 -0.8011899013782227 2116 48-49 0.07898034781301888 2116 50-51 0.36673327274353795 2116 52-53 0.14286526460062277 2116 54-55 -0.5693965761157465 2116 56-57 -0.8907905896083967 2116 58-59 -1.066457649526523 2116 60-61 -1.5219011472211328 2116 62-63 -0.3406457243285175 2116 64-65 0.15870377414903913 2116 66-67 -0.23501816325909175 2116 68-69 -0.8282132077262219 2116 70-71 -1.4300081785028738 2116 72-73 0.7282572474587923 2116 74-75 0.023331558719590362 2116 76 0.7705560015848754 2117 1 0.4839858728319122 2117 2 0.24368059698337063 2117 3 0.6438783073615113 2117 4 -0.31406624542965034 2117 5 -0.1906177199919874 2117 6 0.2347161382309899 2117 7 -0.8064945600329736 2117 8 1.1137407085234834 2117 9 0.6623731762392531 2117 10-11 0.2003042431485902 2117 12-13 -0.14816431091733762 2117 14-15 0.3525137337255124 2117 16-17 1.3580860885032955 2117 18-19 0.36467255733276716 2117 20-21 0.765430913801378 2117 22-23 1.220756330039329 2117 24-25 0.051441051324161435 2117 26-27 1.1165180947220321 2117 28-29 0.5295315964672227 2117 30-31 -0.2626971364733208 2117 32-33 0.5729161366754383 2117 34-35 0.7084186766609726 2117 36-37 -0.8942706600770798 2117 38-39 -0.3155476397811583 2117 40-41 -0.6177005718547477 2117 42-43 -0.46596876292037237 2117 44-45 0.015937270620216992 2117 46-47 -0.040053188628611736 2117 48-49 0.8474135275365207 2117 50-51 1.1234537028510658 2117 52-53 0.11137524923963937 2117 54-55 -0.5106408157470845 2117 56-57 0.48305733665888084 2117 58-59 -0.9068954375449536 2117 60-61 -0.1972083668831921 2117 62-63 0.019761341723707915 2117 64-65 0.060201470001576496 2117 66-67 -0.33217638138505023 2117 68-69 0.706349004255344 2117 70-71 0.7285939720347585 2117 72-73 1.53125263916386 2117 74-75 0.9050519888271182 2117 76 0.26402758376613633 2118 1 2.492804214983586 2118 2 1.2454442654137061 2118 3 1.4582522050687423 2118 4 1.643164795134723 2118 5 1.5977420683678005 2118 6 2.068490388671904 2118 7 2.8381262512545042 2118 8 0.04892589370867029 2118 9 0.9423555395549492 2118 10-11 1.4575793754237232 2118 12-13 2.684728105308409 2118 14-15 2.3992509471293957 2118 16-17 1.9275305329477384 2118 18-19 1.735483844810716 2118 20-21 1.4104926421964414 2118 22-23 1.4952272295102347 2118 24-25 2.4482664481495604 2118 26-27 1.9370648319354373 2118 28-29 1.3117185453208364 2118 30-31 2.180424556648372 2118 32-33 2.0879073183332864 2118 34-35 2.36891250382147 2118 36-37 1.976320168847085 2118 38-39 2.264478815245294 2118 40-41 0.8483488108613066 2118 42-43 1.5554157167974338 2118 44-45 2.3854258067754195 2118 46-47 1.0207933722179519 2118 48-49 1.9933570901467483 2118 50-51 1.705243826307857 2118 52-53 1.3626980005623928 2118 54-55 2.653821265381662 2118 56-57 2.6261349380698142 2118 58-59 2.5245419522257713 2118 60-61 2.089608211600048 2118 62-63 3.1103698073321766 2118 64-65 2.1706512054513105 2118 66-67 0.3940140948054278 2118 68-69 2.9171073816803883 2118 70-71 1.787235947343401 2118 72-73 0.4805471717917271 2118 74-75 2.349496433271561 2118 76 2.0043274073992947 2119 1 0.5023008911469304 2119 2 -0.009066655763884768 2119 3 0.399372812856015 2119 4 0.17915719779379202 2119 5 0.3646936353193695 2119 6 1.4766575301723819 2119 7 0.4913076377692214 2119 8 0.9252791700619447 2119 9 1.010541674407751 2119 10-11 0.6743884922328398 2119 12-13 0.7996378868848595 2119 14-15 0.07778845900023867 2119 16-17 0.0959066013238079 2119 18-19 1.0553318979921045 2119 20-21 -0.5845690861986235 2119 22-23 0.3895292238122252 2119 24-25 0.060232260115370195 2119 26-27 0.3335510617550028 2119 28-29 0.6412532081888358 2119 30-31 -0.35262387640005954 2119 32-33 -0.19082012706082452 2119 34-35 -0.6274787592364639 2119 36-37 -0.39253073333714994 2119 38-39 -0.08139013062364953 2119 40-41 0.0758891717349961 2119 42-43 0.5643425923909859 2119 44-45 -1.3012788465959026 2119 46-47 -0.2393205878960103 2119 48-49 -0.38024215011915885 2119 50-51 0.47922293362029933 2119 52-53 0.8641225019868948 2119 54-55 -0.22804008314635027 2119 56-57 0.21226979087133557 2119 58-59 0.30326939761988214 2119 60-61 0.28182093714611156 2119 62-63 0.15025584721821517 2119 64-65 1.2883150231151284 2119 66-67 0.7145269153182454 2119 68-69 0.9543343522406929 2119 70-71 0.209363202803992 2119 72-73 -0.09713563922441537 2119 74-75 0.6794109631860898 2119 76 0.5631118328503852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 29.0 13 273.0 14 735.0 15 1180.0 16 1519.0 17 1683.0 18 1859.0 19 1741.0 20 1458.0 21 1226.0 22 919.0 23 651.0 24 582.0 25 555.0 26 535.0 27 592.0 28 679.0 29 735.0 30 930.0 31 1163.0 32 1526.0 33 2133.0 34 3049.0 35 4696.0 36 8256.0 37 9458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.521148825065275 22.625587467362923 16.810443864229764 30.042819843342038 2 20.373715353472438 22.05128205128205 33.69251531194851 23.882487283297 3 14.48146994705699 24.93511886224437 34.354821966158 26.228589224540645 4 10.478780832156797 20.52570384519558 34.62752263101071 34.36799269163691 5 9.928371223917782 33.54510536696772 31.809405169729054 24.717118239385446 6 23.81239099742546 36.61448384685657 17.577443733909142 21.99568142180882 7 20.060209695837226 30.889650160905223 19.983390428734555 29.066749714522995 8 20.992421883110143 34.41918405481159 18.694072459254645 25.894321602823627 9 21.378594415031664 19.975085643101835 19.33561714938233 39.31070279248417 10-11 20.270943631267517 26.687428630748467 26.453856534828198 26.587771203155818 12-13 19.42696979134226 24.569708294404652 25.629606560780648 30.373715353472434 14-15 19.389598255995015 26.271151250908336 22.973113256514065 31.36613723658258 16-17 20.88861206270113 28.236271151250907 23.807744212602515 27.067372573445446 18-19 19.366760095505033 27.00197238658777 24.010173362400085 29.62109415550711 20-21 18.35669054292536 27.61133603238866 24.64756565971141 29.38440776497457 22-23 21.290356067684 27.08709643932316 23.866915810235646 27.755631682757187 24-25 19.549465379424895 29.743589743589745 23.733001141908026 26.973943735077338 26-27 18.289214159659505 29.93563791134641 25.333748572614965 26.441399356379115 28-29 19.854666251427386 28.06290875116786 27.11304889442541 24.969376102979343 30-31 19.72178968130385 27.76601266479809 27.142115644139935 25.37008200975812 32-33 17.696460085124052 30.58341119069864 25.947264611232224 25.772864112945083 34-35 18.989930447420324 29.071940205543445 25.875635835150007 26.062493511886224 36-37 19.572303539914877 27.664279040797258 26.71649538046299 26.04692203882487 38-39 18.813453752725007 26.912695941036024 28.165680473372777 26.10816983286619 40-41 18.62659607598879 26.786047960137026 26.962524654832347 27.624831309041838 42-43 16.98536281532233 26.63240942593169 28.533167237620678 27.849060521125296 44-45 17.529326274265543 27.790927021696255 28.050451572718778 26.629295131319424 46-47 17.66324094259317 28.557043496314748 26.98536281532233 26.79435274576975 48-49 17.417211668223814 29.267102667912386 25.94622651302813 27.369459150835667 50-51 17.56358351500052 28.381604899823525 25.572511159555695 28.482300425620267 52-53 16.927229315893282 27.994394269697914 26.355237205439636 28.72313920896917 54-55 17.01027717222049 28.291290356067684 25.53513962420845 29.163292847503374 56-57 18.117927955984637 27.818955673206684 25.4988061870653 28.56431018374338 58-59 17.72241254022631 27.972594207412023 25.458320357105784 28.846672895255892 60-61 17.060105886016817 27.03726772552684 25.44171078584034 30.460915602616005 62-63 17.37776393646839 27.760822173777637 25.43340600020762 29.428007889546354 64-65 18.053565867331052 28.774006020969583 24.025744835461435 29.146683276237933 66-67 17.805460396553514 29.825599501712862 23.614657946641753 28.75428215509187 68-69 17.75563168275719 29.79238035918198 23.46205751064051 28.989930447420324 70-71 18.63282466521333 30.67476383265857 22.796636561818747 27.895774940309355 72-73 17.548012041939167 29.80172324301879 22.535035814388042 30.115228900654 74-75 17.576040693449603 29.54635108481262 23.084189764351706 29.793418457386068 76 17.616526523409114 27.68400290667497 23.68524862451988 31.014221945396038 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 1.5 14 3.0 15 4.5 16 6.0 17 6.0 18 6.5 19 9.5 20 13.5 21 15.0 22 17.0 23 29.5 24 53.0 25 66.0 26 89.5 27 134.5 28 209.5 29 263.0 30 303.5 31 422.5 32 594.5 33 688.0 34 786.5 35 980.5 36 1213.0 37 1350.0 38 1528.0 39 1915.5 40 2341.0 41 2734.5 42 2912.0 43 2980.5 44 3227.5 45 3389.5 46 3373.0 47 3410.0 48 3331.0 49 3126.5 50 3038.0 51 2851.5 52 2447.0 53 2047.5 54 1866.0 55 1794.0 56 1523.5 57 1166.5 58 1008.0 59 880.5 60 653.0 61 488.5 62 424.0 63 375.5 64 263.5 65 193.5 66 151.5 67 116.0 68 105.0 69 81.0 70 59.0 71 50.0 72 45.5 73 33.5 74 26.0 75 26.0 76 20.5 77 13.0 78 8.5 79 6.0 80 7.5 81 9.0 82 6.0 83 3.0 84 3.0 85 4.0 86 6.0 87 7.0 88 4.5 89 2.5 90 2.0 91 2.5 92 4.0 93 2.5 94 2.5 95 3.5 96 3.0 97 3.5 98 5.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.602096958372262 2 0.0 3 0.0 4 0.002076196408180214 5 0.0 6 0.002076196408180214 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48165.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.09176788124157 #Duplication Level Percentage of deduplicated Percentage of total 1 83.30789390317656 60.05813349942905 2 8.253895112749476 11.900757811688987 3 3.548080522996285 7.6736219246340704 4 1.8604383261814936 5.3648915187376724 5 1.1145350344152292 4.017440049828714 6 0.7142240013823691 3.089380255372158 7 0.41471071048008523 2.0928059794456555 8 0.2937534199233937 1.6941762690750546 9 0.13535696800391672 0.8782310806602304 >10 0.35711200069118454 3.230561611128413 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006228589224540642 0.0 2 0.0 0.0 0.0 0.006228589224540642 0.0 3 0.0 0.0 0.0 0.006228589224540642 0.0 4 0.0 0.0 0.0 0.006228589224540642 0.0 5 0.0 0.0 0.0 0.006228589224540642 0.0 6 0.0 0.0 0.0 0.006228589224540642 0.0 7 0.0 0.0 0.0 0.006228589224540642 0.0 8 0.0 0.0 0.0 0.006228589224540642 0.0 9 0.0 0.0 0.0 0.008304785632720855 0.0 10 0.0 0.0 0.0 0.008304785632720855 0.0 11 0.0 0.0 0.0 0.008304785632720855 0.0 12 0.0 0.0 0.0 0.008304785632720855 0.0 13 0.0 0.0 0.0 0.008304785632720855 0.0 14 0.0 0.0 0.0 0.008304785632720855 0.0 15 0.0 0.0 0.0 0.008304785632720855 0.0 16 0.0 0.0 0.0 0.01038098204090107 0.0 17 0.0 0.0 0.0 0.01660957126544171 0.0 18 0.0 0.0 0.0 0.01660957126544171 0.0 19 0.0 0.0 0.0 0.022838160489982353 0.0 20 0.0 0.0 0.0 0.022838160489982353 0.0 21 0.0 0.0 0.0 0.024914356898162567 0.0 22 0.0 0.0 0.0 0.024914356898162567 0.0 23 0.0 0.0 0.0 0.02699055330634278 0.0 24 0.0 0.0 0.0 0.02699055330634278 0.0 25 0.0 0.0 0.0 0.02699055330634278 0.0 26 0.0 0.0 0.0 0.029066749714522992 0.0 27 0.0 0.0 0.0 0.03321914253088342 0.0 28 0.0 0.0 0.0 0.04360012457178449 0.0 29 0.0 0.0 0.0 0.05190491020450535 0.0 30 0.0 0.0 0.0 0.0602096958372262 0.0 31 0.0 0.0 0.0 0.08304785632720856 0.0 32 0.0 0.0 0.0 0.11419080244991177 0.0 33 0.0 0.0 0.0 0.1349527665317139 0.0 34 0.0 0.0 0.0 0.17440049828713797 0.0 35 0.0 0.0 0.0 0.23253399771618394 0.0 36 0.0 0.0 0.0 0.30104847918613103 0.0 37 0.0 0.0 0.0 0.40693449600332193 0.0 38 0.0 0.0 0.0 0.6062493511886224 0.0 39 0.0 0.0 0.0 0.7951832243330219 0.0 40 0.0 0.0 0.0 0.9924218831101422 0.0 41 0.0 0.0 0.0 1.2706322018062908 0.0 42 0.0 0.0 0.0 1.5114709851551957 0.0 43 0.0 0.0 0.0 1.7813765182186234 0.0 44 0.0 0.0 0.0 2.0429772656493306 0.0 45 0.0 0.0 0.0 2.3730924945499843 0.0 46 0.0 0.0 0.0 2.640921831205232 0.0 47 0.0 0.0 0.0 2.9170559534932003 0.0 48 0.0 0.0 0.0 3.288695110557459 0.0 49 0.0 0.0 0.0 3.612581750233572 0.0 50 0.0 0.0 0.0 3.8783348904806396 0.0 51 0.0 0.0 0.0 4.233364476279456 0.0 52 0.0 0.0 0.0 4.551022526731029 0.0 53 0.0 0.0 0.0 4.922661683795287 0.0 54 0.0 0.0 0.0 5.202948198899616 0.0 55 0.0 0.0 0.0 5.551749195473892 0.0 56 0.0 0.0 0.0 5.7801308003737155 0.0 57 0.0 0.0 0.0 6.045883940620783 0.0 58 0.0 0.0 0.0 6.282570331153327 0.0 59 0.0 0.0 0.0 6.5711616318903765 0.0 60 0.0 0.0 0.0 6.826533790096543 0.0 61 0.0 0.0 0.0 7.18986816152808 0.0 62 0.0 0.0 0.0 7.517907194020554 0.0 63 0.0 0.0 0.0 7.850098619329389 0.0 64 0.0 0.0 0.0 8.167756669780962 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCATAA 15 0.0022128567 69.99274 20 CCTGACC 15 0.0022128567 69.99274 8 AGACTGG 20 0.0069146953 52.49455 35 TTTAAAG 20 0.0069146953 52.49455 26 TTTGGAG 20 0.0069146953 52.49455 31 GGGCCTA 20 0.0069146953 52.49455 55 TTAAAGG 20 0.0069146953 52.49455 27 AGTAGTG 20 0.0069146953 52.49455 5 GAAGGGC 20 0.0069146953 52.49455 36 AGAAGCC 40 0.0023977847 34.996365 69 TATCCAT 40 0.0023977847 34.996365 42 CCGACGA 205 1.2111179E-5 15.364259 18 GCCGACG 215 3.139336E-4 13.021903 17 TGCCGAC 250 0.0011164644 11.198837 16 GCTGCCG 300 4.2788553E-4 10.49891 14 ACGCTGC 310 0.0066482835 9.031321 12 CGCTGCC 320 0.008626327 8.749091 13 >>END_MODULE