FastQCFastQC Report
Wed 25 May 2016
SRR1780396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48165
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT34117.081905948302709RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT15493.216028236271151No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC11622.4125402263054085No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC5081.0547077753555485No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT2770.5751064050659193No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1630.33842001453337484No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1580.3280390324924738No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT1230.25537215820616627No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT1100.22838160489982354No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT1090.22630540849164332No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC1060.22007681926710268No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC820.1702481054707775No Hit
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG800.1660957126544171No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC680.14118135575625454No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC660.1370289629398941No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT650.1349527665317139No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG600.12457178449081284No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT600.12457178449081284No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC600.12457178449081284No Hit
CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC520.10796221322537111No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTC150.002211945870.057
CTATGCG150.002211945870.041
ACCGGCG150.002211945870.055
TAAATGG150.002211945870.017
CACCGGC150.002211945870.054
GCGTCAA150.002211945870.059
AAAATGT150.002211945870.067
CGTCAAA150.002211945870.060
CCACCGG150.002211945870.053
TTTACAC2150.063.4883732
CTATACA1150.060.8695641
CTTTACA2250.060.666661
TTACACA2350.058.0851063
CTTTATA252.3680243E-455.9999962
GAACAAG252.3680243E-455.99999625
CGGACAC200.006911855752.528
CATGGTT200.006911855752.514
TCACATC200.006911855752.55
TAGACAC200.006911855752.55
GCTATGC200.006911855752.540
Produced by FastQC (version 0.11.5)