##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780396_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48165 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22983494238555 34.0 34.0 34.0 27.0 34.0 2 32.987854251012145 34.0 34.0 34.0 31.0 34.0 3 33.29897228277795 34.0 34.0 34.0 33.0 34.0 4 33.361714938233156 34.0 34.0 34.0 33.0 34.0 5 33.383681096231705 34.0 34.0 34.0 34.0 34.0 6 37.03600124571784 38.0 37.0 38.0 36.0 38.0 7 36.68884044430603 38.0 37.0 38.0 34.0 38.0 8 36.552122910827364 38.0 37.0 38.0 34.0 38.0 9 36.369396864943425 38.0 37.0 38.0 34.0 38.0 10-11 36.01497975708502 38.0 36.5 38.0 32.5 38.0 12-13 35.86161112841275 38.0 36.0 38.0 31.5 38.0 14-15 36.029025225786356 38.0 36.5 38.0 32.5 38.0 16-17 36.15929616941763 38.0 37.0 38.0 33.0 38.0 18-19 36.203477628983705 38.0 37.0 38.0 34.0 38.0 20-21 35.94067268763625 38.0 36.5 38.0 31.5 38.0 22-23 35.92021177203364 38.0 36.5 38.0 32.5 38.0 24-25 35.09124883213952 37.5 35.0 38.0 28.5 38.0 26-27 35.307806498494756 37.0 36.0 38.0 31.0 38.0 28-29 35.099439426969795 37.5 35.5 38.0 29.0 38.0 30-31 34.58504100487906 37.0 34.0 38.0 26.0 38.0 32-33 35.207567735907816 37.5 35.5 38.0 31.0 38.0 34-35 34.882549569189244 37.0 35.0 38.0 27.0 38.0 36-37 34.958112737464965 37.0 35.5 38.0 27.0 38.0 38-39 34.5161424270736 37.0 34.5 38.0 25.5 38.0 40-41 34.33394581127375 37.0 34.0 38.0 25.5 38.0 42-43 35.02671026679124 37.0 35.0 38.0 29.0 38.0 44-45 35.287189868161526 37.0 36.0 38.0 29.0 38.0 46-47 35.446434132668955 38.0 36.0 38.0 31.0 38.0 48-49 35.15048271566491 37.5 35.5 38.0 29.0 38.0 50-51 35.33141285165577 37.5 36.0 38.0 31.0 38.0 52-53 35.14432679331465 37.0 36.0 38.0 27.0 38.0 54-55 35.369614865566284 38.0 36.0 38.0 31.0 38.0 56-57 35.38582995951417 38.0 36.0 38.0 31.0 38.0 58-59 35.47082944046507 38.0 36.0 38.0 31.0 38.0 60-61 35.15683587667393 37.5 35.5 38.0 29.0 38.0 62-63 35.58230042562026 38.0 36.0 38.0 31.0 38.0 64-65 35.56233779715561 38.0 36.0 38.0 31.0 38.0 66-67 34.45748987854251 37.0 34.0 38.0 26.0 38.0 68-69 32.564984947576036 37.0 31.5 38.0 16.0 38.0 70-71 31.56057303020866 37.0 28.0 38.0 11.0 38.0 72-73 30.70516972905637 36.5 25.0 38.0 11.0 38.0 74-75 31.182653379009654 37.0 26.0 38.0 11.0 38.0 76 30.926295027509603 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8087237982326272 1101 2 0.7739403132181337 1101 3 0.06273098083650552 1101 4 0.46293119406789884 1101 5 0.5371149824826276 1101 6 -0.8607198712485555 1101 7 0.21993900036881087 1101 8 0.31802709681141295 1101 9 0.5769860053478126 1101 10-11 0.5610294594466296 1101 12-13 0.653963931012818 1101 14-15 1.1643891820696552 1101 16-17 0.31545004621873574 1101 18-19 -0.145142154947429 1101 20-21 0.5251464866175368 1101 22-23 1.134471391572852 1101 24-25 -1.6612903614269996 1101 26-27 -0.0058520329775006985 1101 28-29 1.5048497838913022 1101 30-31 1.883604225771208 1101 32-33 0.41796115692650204 1101 34-35 1.0004054012594779 1101 36-37 0.6989196642549018 1101 38-39 -0.3976697348601732 1101 40-41 1.844348120078159 1101 42-43 0.9386257788055303 1101 44-45 0.37762954562730755 1101 46-47 1.3932171092305268 1101 48-49 -1.0423561765956322 1101 50-51 -1.0272655347959514 1101 52-53 -0.9294598839356212 1101 54-55 -0.48027211181256035 1101 56-57 -1.059613627461033 1101 58-59 -1.010358783586966 1101 60-61 -1.9683489157226575 1101 62-63 -0.5381075794043255 1101 64-65 -1.4231292097853299 1101 66-67 -1.2981972636844787 1101 68-69 0.4454135019183738 1101 70-71 -0.3901172140032543 1101 72-73 -1.4262727538189743 1101 74-75 0.06309864507089102 1101 76 -0.7826486410267535 1102 1 -0.9221585547085454 1102 2 0.4312932543946033 1102 3 0.15222677915583205 1102 4 0.31923371507630094 1102 5 0.39908977239859666 1102 6 0.35671710354135655 1102 7 0.26279614322595535 1102 8 0.834413651433259 1102 9 0.7883305431629424 1102 10-11 1.2697479468415835 1102 12-13 0.8868420822733185 1102 14-15 0.01071271148142472 1102 16-17 0.6398197941178978 1102 18-19 -0.24577240704826409 1102 20-21 0.7767221168696352 1102 22-23 0.8472865176232816 1102 24-25 -4.800786159746327 1102 26-27 -0.9946125371791865 1102 28-29 0.1574758343114695 1102 30-31 2.045368931653556 1102 32-33 0.8612384678508747 1102 34-35 1.0824432163855349 1102 36-37 -2.552025713896356 1102 38-39 0.10611177774487146 1102 40-41 1.5880455990697584 1102 42-43 1.4096341821668688 1102 44-45 1.459772402770163 1102 46-47 1.7591834957851518 1102 48-49 -2.979856176595632 1102 50-51 -0.05447141714889625 1102 52-53 0.05530902362740875 1102 54-55 0.5820178041538284 1102 56-57 0.8347141036314056 1102 58-59 0.30697314918614893 1102 60-61 -5.9981808484957675 1102 62-63 -2.436426907135413 1102 64-65 0.4541817145844149 1102 66-67 1.6420338287524956 1102 68-69 -0.19555288463624976 1102 70-71 -0.32499116358308555 1102 72-73 -3.7689198126425048 1102 74-75 -1.4779727835005367 1102 76 0.9618891740992979 1103 1 1.18132981738448 1103 2 0.8452467427666974 1103 3 0.15438624759437403 1103 4 0.6137519875015514 1103 5 0.3690233272158707 1103 6 0.4705044789566415 1103 7 1.0280785352525328 1103 8 1.0427192992737915 1103 9 0.3635797125981526 1103 10-11 0.44175791361899286 1103 12-13 -0.6342542632416297 1103 14-15 -0.0485896140999742 1103 16-17 -0.05544598661300881 1103 18-19 0.6496594866394432 1103 20-21 -0.11738087316358303 1103 22-23 0.5306752219422108 1103 24-25 -3.1221316746964902 1103 26-27 -0.4496789823619025 1103 28-29 0.17815689743439833 1103 30-31 1.2256014897930925 1103 32-33 1.4820856439306098 1103 34-35 0.9473103260200801 1103 36-37 -0.9126569431322338 1103 38-39 -0.7158981890325364 1103 40-41 0.21678314059799675 1103 42-43 -0.4510801035474117 1103 44-45 1.3776295456273076 1103 46-47 1.2950638944562485 1103 48-49 -2.229856176595632 1103 50-51 -0.40970397528842994 1103 52-53 0.09849839239817015 1103 54-55 -0.21291575066344848 1103 56-57 0.5732523096114761 1103 58-59 -0.2845550900165108 1103 60-61 -4.413629353479159 1103 62-63 -1.101377073248372 1103 64-65 0.5240322129232879 1103 66-67 0.17384445998172993 1103 68-69 -0.005851887958513657 1103 70-71 0.3631317600049542 1103 72-73 -3.756129114968086 1103 74-75 -2.136029261905854 1103 76 -2.206383251149873 1104 1 -0.21446624701624017 1104 2 0.37744710054845143 1104 3 0.5861095630386188 1104 4 -0.008788262945678582 1104 5 -0.037357114048290896 1104 6 -0.06452831770405965 1104 7 0.48916976959957736 1104 8 0.9684795854991961 1104 9 0.9612243160567147 1104 10-11 -0.6306183535247172 1104 12-13 0.64123768666893 1104 14-15 0.8017383525070656 1104 16-17 0.4087758380739359 1104 18-19 0.7142092779334206 1104 20-21 -0.1889372237897078 1104 22-23 0.4259495212862774 1104 24-25 0.738407979447814 1104 26-27 1.5926585250918777 1104 28-29 1.7858641126997483 1104 30-31 1.1928048290894537 1104 32-33 1.1345901162025243 1104 34-35 0.6922417511840635 1104 36-37 1.9895493776787276 1104 38-39 1.5872473088804 1104 40-41 2.2904265514507074 1104 42-43 1.0264839990166905 1104 44-45 1.5122449302426944 1104 46-47 1.8687073053089662 1104 48-49 0.13873356699411232 1104 50-51 0.825015762338289 1104 52-53 0.36116982948821175 1104 54-55 0.6934646906007131 1104 56-57 0.2707031146204173 1104 58-59 -0.878100110887118 1104 60-61 0.4234308731159544 1104 62-63 0.6841774884689755 1104 64-65 0.8724967328994353 1104 66-67 0.6002755869942504 1104 68-69 2.101333562250197 1104 70-71 3.043140704548783 1104 72-73 1.9913365976139055 1104 74-75 0.7858550553273034 1104 76 0.9684825806927044 1105 1 1.0381862728776596 1105 2 0.8243967026704695 1105 3 0.5629000139670026 1105 4 0.2294964408562663 1105 5 -0.34549150838957843 1105 6 0.33290757973183815 1105 7 0.9526976210584692 1105 8 -0.5998228017687097 1105 9 1.022895403589871 1105 10-11 1.1276707711437197 1105 12-13 -0.6177145679729819 1105 14-15 0.5385862746998171 1105 16-17 -0.12183866236814822 1105 18-19 0.3960174123277582 1105 20-21 0.4150636603835949 1105 22-23 0.28127666540652996 1105 24-25 0.9894026744080264 1105 26-27 0.46367974525102795 1105 28-29 1.1298075256086832 1105 30-31 1.3126103109639047 1105 32-33 1.4039314070955342 1105 34-35 -0.8265469313977221 1105 36-37 1.0437049921791726 1105 38-39 -0.3759489775917473 1105 40-41 -0.119056207169983 1105 42-43 -0.10625251734051488 1105 44-45 1.1649858674663918 1105 46-47 1.3744544317457397 1105 48-49 -0.2858906593542514 1105 50-51 1.0234021460694933 1105 52-53 0.8003829152530244 1105 54-55 0.944127820574181 1105 56-57 1.2045006389351798 1105 58-59 0.6321784037017437 1105 60-61 1.1168437820461037 1105 62-63 -0.1675024407971506 1105 64-65 1.0270060167190564 1105 66-67 0.2363277532188377 1105 68-69 0.6351531908974124 1105 70-71 1.7786623667945847 1105 72-73 1.5911376586218644 1105 74-75 0.9646791048410073 1105 76 1.5031863826378817 1106 1 -0.793587126137119 1106 2 0.02415039725174495 1106 3 0.3093696362986904 1106 4 0.4120908579334426 1106 5 0.4133754866843091 1106 6 0.4067171035413608 1106 7 -0.8443467139169059 1106 8 0.20584222286183262 1106 9 -0.7116694568370576 1106 10-11 0.5840336611273003 1106 12-13 1.3832706537018922 1106 14-15 0.15714128290998985 1106 16-17 0.4326769369750423 1106 18-19 0.8113704500945929 1106 20-21 0.6160078311553505 1106 22-23 0.44728651762327587 1106 24-25 -0.8686433026034734 1106 26-27 0.9732446056779622 1106 28-29 1.325332977168614 1106 30-31 1.1239403602249851 1106 32-33 0.21480989642230242 1106 34-35 0.9288717878141028 1106 36-37 2.0158314289607873 1106 38-39 0.16682606345915474 1106 40-41 1.4273313133554737 1106 42-43 0.5382056107383022 1106 44-45 1.124058117055874 1106 46-47 1.3413263529280144 1106 48-49 1.780858109118654 1106 50-51 0.1455285828511066 1106 52-53 -0.04469097637259267 1106 54-55 0.8748749470109658 1106 56-57 0.7347141036314042 1106 58-59 1.4176874349004294 1106 60-61 1.433962008647086 1106 62-63 1.495715950007444 1106 64-65 -1.267246856844153 1106 66-67 0.4527481144667789 1106 68-69 2.243732829649467 1106 70-71 1.535723122131202 1106 72-73 1.113223044500355 1106 74-75 -0.41725849778625346 1106 76 0.540460602670727 1107 1 1.0712149392673567 1107 2 -0.3120802395812987 1107 3 0.14086705454309367 1107 4 0.2859290679162285 1107 5 0.14599166568603295 1107 6 0.22848990904910238 1107 7 0.7892161088025489 1107 8 0.18974927966045385 1107 9 -0.20969011088180878 1107 10-11 0.7366584459809999 1107 12-13 -0.09891523270086111 1107 14-15 -0.09380536080773538 1107 16-17 0.7647767648579986 1107 18-19 0.9141243227279787 1107 20-21 1.3077031839952866 1107 22-23 -0.04350522075882424 1107 24-25 0.02111573354110874 1107 26-27 -0.11096365593994051 1107 28-29 1.3814001028140552 1107 30-31 0.8887424256294594 1107 32-33 1.1495345091589613 1107 34-35 -0.2487529970395954 1107 36-37 1.6763735976354894 1107 38-39 0.26630971234039436 1107 40-41 0.5529767522539188 1107 42-43 1.0700042338019884 1107 44-45 0.7601596661092387 1107 46-47 1.0936069037025362 1107 48-49 0.6150233414766575 1107 50-51 1.5175165346583341 1107 52-53 0.8793193506497801 1107 54-55 0.6185496458061479 1107 56-57 1.1695678041477535 1107 58-59 0.7363191044357151 1107 60-61 1.3128346420377994 1107 62-63 0.648727998200215 1107 64-65 0.7707049503847543 1107 66-67 -0.6431210765831352 1107 68-69 1.1953249122656402 1107 70-71 -0.5248620758033944 1107 72-73 0.04342958494785876 1107 74-75 -1.503919427218264 1107 76 0.008102599228383411 1108 1 -0.8143272294073434 1108 2 0.56743783270786 1108 3 0.0625538015310454 1108 4 0.5268929233379183 1108 5 0.12791937652940533 1108 6 -0.09681129577017344 1108 7 -0.33126581890829954 1108 8 -0.8102507203395461 1108 9 -0.01691902654446409 1108 10-11 0.9926825423665449 1108 12-13 1.4553016347690146 1108 14-15 0.9754717476260026 1108 16-17 -0.38582564478055303 1108 18-19 0.13701588899304085 1108 20-21 1.12999234062179 1108 22-23 0.11914538165081723 1108 24-25 0.8885856130591776 1108 26-27 -0.6169877523254854 1108 28-29 -1.0884794152582344 1108 30-31 0.23814001599090773 1108 32-33 0.9748357139782371 1108 34-35 0.9831747138037699 1108 36-37 1.8209519108884962 1108 38-39 1.437996459328346 1108 40-41 1.3692418124948844 1108 42-43 -0.7854174529450049 1108 44-45 1.4589548468321212 1108 46-47 1.286377988039881 1108 48-49 1.1782763535248506 1108 50-51 0.7343840045378514 1108 52-53 -0.07549992645864734 1108 54-55 -0.44169131804927275 1108 56-57 0.015953346316429418 1108 58-59 0.4561986225080048 1108 60-61 0.9031960878209304 1108 62-63 1.3414990825375668 1108 64-65 0.9755242274931959 1108 66-67 0.8629030198024097 1108 68-69 1.6862887676873228 1108 70-71 -1.151368099899777 1108 72-73 -0.34813668011238263 1108 74-75 -1.0942808730013986 1108 76 -0.2629817381210131 1109 1 -1.0990816316316234 1109 2 0.49924197234332013 1109 3 0.37457110150015893 1109 4 0.15146814731073022 1109 5 -0.101459678150853 1109 6 -0.38504113821688435 1109 7 -0.5300609996311891 1109 8 0.6223257393453494 1109 9 0.9676345724669702 1109 10-11 0.6738688259624652 1109 12-13 0.3591864046176454 1109 14-15 0.8401998909686057 1109 16-17 1.3158271201252205 1109 18-19 0.9545938933180409 1109 20-21 1.407216622364146 1109 22-23 0.2624879828247444 1109 24-25 -0.3769766359367992 1109 26-27 -0.29836711593375753 1109 28-29 -1.7077256308899962 1109 30-31 0.6415227778073955 1109 32-33 0.5608721674845683 1109 34-35 -0.064168505226192 1109 36-37 1.7107032238325814 1109 38-39 1.4141703858034802 1109 40-41 0.5276060386301964 1109 42-43 1.5489198964525883 1109 44-45 1.0058346738324317 1109 46-47 0.9520406386422948 1109 48-49 0.5041181823787255 1109 50-51 0.126297813620333 1109 52-53 -0.39844555512717506 1109 54-55 -0.957176335040316 1109 56-57 -0.107502013584714 1109 58-59 -0.15374113652814003 1109 60-61 1.141379591064677 1109 62-63 0.33802364231513593 1109 64-65 0.9141633995661067 1109 66-67 0.5233525100711702 1109 68-69 -0.8698202839036497 1109 70-71 -0.5754490390409615 1109 72-73 -0.47340699212968573 1109 74-75 -0.8455552010829557 1109 76 -0.15331229110216782 1110 1 -1.533903521151501 1110 2 -0.7807872825181477 1110 3 -0.04317111154406916 1110 4 -0.215809429698389 1110 5 0.296836656387093 1110 6 -0.2420364918565312 1110 7 0.906180611107068 1110 8 1.2254491260257794 1110 9 1.4220314060584371 1110 10-11 -0.18248119985064903 1110 12-13 0.8516790909022731 1110 14-15 1.1565180806089401 1110 16-17 0.2808073300718732 1110 18-19 0.03414130340235744 1110 20-21 1.074421062219585 1110 22-23 -0.2626367805550558 1110 24-25 -0.5579050475699177 1110 26-27 0.940382668956957 1110 28-29 0.33753336067772466 1110 30-31 0.13093940145220984 1110 32-33 1.160854958742533 1110 34-35 1.3599839642282916 1110 36-37 2.223717335001055 1110 38-39 1.0392134076585862 1110 40-41 -1.1639917930683055 1110 42-43 0.3542890239693719 1110 44-45 1.6460859214662307 1110 46-47 1.1986849339443069 1110 48-49 1.43876798447819 1110 50-51 0.24032724056922206 1110 52-53 1.1775525519112051 1110 54-55 0.3106132020445216 1110 56-57 1.6042730681568145 1110 58-59 -0.1680028814945871 1110 60-61 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-1.0337846783695213 2116 54-55 -0.4560390314836553 2116 56-57 -0.18287422202143233 2116 58-59 -3.0260529644851317 2116 60-61 -3.039348283211588 2116 62-63 0.6107697134483061 2116 64-65 -2.1823006202850195 2116 66-67 -1.0352703187129393 2116 68-69 -2.0314207801815662 2116 70-71 0.4121440130682217 2116 72-73 -0.6917692750080953 2116 74-75 -0.4899927527785728 2116 76 -0.360921885808537 2117 1 1.2278414452914532 2117 2 0.5789123020136557 2117 3 0.13556011248916633 2117 4 -0.5783853325427444 2117 5 -0.7342435609347362 2117 6 0.32100281782707185 2117 7 0.44612947655928537 2117 8 0.1296517466713567 2117 9 -1.0378599330275335 2117 10-11 -0.8957282436346006 2117 12-13 -1.3322055367742962 2117 14-15 -0.7321444313757155 2117 16-17 -1.5280373487392467 2117 18-19 -0.6910105022863604 2117 20-21 -0.09232550217798519 2117 22-23 -0.1372372919005329 2117 24-25 -2.644833778793945 2117 26-27 -2.981517299083947 2117 28-29 -2.2365717847361495 2117 30-31 -0.09748821120358286 2117 32-33 -2.049475817863417 2117 34-35 -0.0746996407573235 2117 36-37 -2.0305971424677836 2117 38-39 1.121587968221057 2117 40-41 0.5642360752602329 2117 42-43 0.3227294202621067 2117 44-45 0.24072478372254835 2117 46-47 -1.4646260280243766 2117 48-49 -1.2834276051670628 2117 50-51 -0.6282809409584189 2117 52-53 0.9184042617226424 2117 54-55 -0.5382202910842722 2117 56-57 -0.0795716106543054 2117 58-59 -1.231122088909089 2117 60-61 -1.1196094199243447 2117 62-63 -0.48523643094493707 2117 64-65 -1.7708182854155865 2117 66-67 -0.8960614093427424 2117 68-69 -1.2681719322552993 2117 70-71 -2.0868959254878447 2117 72-73 -0.38320552692821863 2117 74-75 0.13750340697565377 2117 76 -0.05715844494832112 2118 1 -0.8277141102641039 2118 2 -0.10450921474119923 2118 3 -1.5147926211969 2118 4 -0.19302378051452962 2118 5 -0.20734761737212892 2118 6 -0.22264797582371898 2118 7 0.5193217164181902 2118 8 0.12303799005759686 2118 9 -3.0660786279129013 2118 10-11 -1.3899963212360191 2118 12-13 -1.5162884291905243 2118 14-15 0.696206538641917 2118 16-17 -3.02605322175512 2118 18-19 -1.432633077242265 2118 20-21 -1.337916331102143 2118 22-23 0.5212724082758342 2118 24-25 0.4116036109767762 2118 26-27 -3.323889433122744 2118 28-29 -2.466510056341086 2118 30-31 -1.0256187226674314 2118 32-33 -1.3636292570168536 2118 34-35 -0.4832093409336906 2118 36-37 -4.54250190437255 2118 38-39 -1.3717982751651832 2118 40-41 -1.3932154238579315 2118 42-43 -0.4757714615721014 2118 44-45 -4.94335810869368 2118 46-47 -1.4831445465428956 2118 48-49 -0.5863376580771131 2118 50-51 -2.04428623196371 2118 52-53 -3.538165403180354 2118 54-55 -2.1087670282976774 2118 56-57 -0.5359208170035146 2118 58-59 -2.4268892846762853 2118 60-61 -1.2223431059913565 2118 62-63 -3.919098864807374 2118 64-65 -0.3931727827700797 2118 66-67 -0.6061584111064136 2118 68-69 -0.8155705213205522 2118 70-71 -0.7758289060874937 2118 72-73 0.2616357429130538 2118 74-75 0.7891788919844736 2118 76 0.13728599949612175 2119 1 -0.10292778547777459 2119 2 -1.7071682840669347 2119 3 -0.5049160779870263 2119 4 -0.6703267244841413 2119 5 -0.7706904473816252 2119 6 -0.861964215139956 2119 7 -0.8531379227081146 2119 8 -0.7584434914238827 2119 9 -1.845185940353545 2119 10-11 -1.8530542509606107 2119 12-13 -1.0722238517926144 2119 14-15 -1.0950565192878088 2119 16-17 -1.7585318542337518 2119 18-19 -0.8518163630922189 2119 20-21 -3.379321839174324 2119 22-23 0.1445392648760233 2119 24-25 0.49289515893499214 2119 26-27 -0.4605466031132437 2119 28-29 -0.3205461437105015 2119 30-31 0.22613816242278517 2119 32-33 -0.7750252684128682 2119 34-35 -0.34750183855952343 2119 36-37 -1.132245494116134 2119 38-39 -3.390316793683702 2119 40-41 -3.8127785767544182 2119 42-43 -1.328003180470489 2119 44-45 -0.06083199283423113 2119 46-47 -0.2825747459730863 2119 48-49 -2.2817792535187067 2119 50-51 0.36283627515879857 2119 52-53 -0.06447119615280883 2119 54-55 -1.0462788991428766 2119 56-57 -3.327373808456503 2119 58-59 -1.4941257519127547 2119 60-61 0.09522574491082025 2119 62-63 -1.2888994346079414 2119 64-65 -1.1057083953056974 2119 66-67 -0.21318595146728825 2119 68-69 0.8885130494296831 2119 70-71 -1.9318592954512148 2119 72-73 -1.2093044280271172 2119 74-75 -1.10901673954449 2119 76 -1.1892097269996036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 1.0 20 3.0 21 4.0 22 11.0 23 35.0 24 55.0 25 103.0 26 242.0 27 411.0 28 666.0 29 1035.0 30 1522.0 31 2441.0 32 3493.0 33 4851.0 34 6551.0 35 7584.0 36 9382.0 37 9774.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.70071628776082 27.09436312675179 10.696563894944463 31.508356690542925 2 15.660749506903354 17.88227966365618 47.64247897851136 18.814491850929098 3 14.211564413993564 31.14086992629503 34.53960344648604 20.10796221322537 4 9.961590366448666 22.50389286826534 36.35004671441918 31.184470050866814 5 9.32212187272916 44.65690854354822 34.493927125506076 11.527042458216547 6 19.82767569812104 37.83452714626804 16.628257033115332 25.70954012249559 7 19.71348489567113 40.670611439842205 19.25256929305512 20.363334371431538 8 17.46911657842832 34.04131630852278 26.234817813765183 22.254749299283713 9 17.992318073289734 20.053981106612685 18.206166303332296 43.747534516765285 10-11 18.125194643413266 24.05481158517596 31.82497664279041 25.99501712862037 12-13 23.272085539292018 18.471919443579363 27.162877608221734 31.093117408906885 14-15 24.597736945915084 24.509498598567426 19.542198691996262 31.350565763521228 16-17 23.701858195785324 26.68639053254438 19.78615176995744 29.825599501712862 18-19 20.632201806290876 27.37257344544794 20.267829336655247 31.727395411605936 20-21 21.530156752828816 24.384926814076614 20.55330634278003 33.531610090314544 22-23 24.997404754489775 29.862971037060106 20.828402366863905 24.311221841586214 24-25 23.244056887781582 32.94716080141181 20.61247794041316 23.196304370393438 26-27 25.1209384407765 28.14803280390325 20.67476383265857 26.056264922661683 28-29 24.800166095712655 22.32637807536593 21.56752828817606 31.305927540745355 30-31 28.775044119173675 25.74691165784283 20.842935741721167 24.63510848126233 32-33 25.575625454167966 29.589951209384406 20.85331672376207 23.98110661268556 34-35 18.566386380151563 32.93989411398318 26.024083878334892 22.469635627530366 36-37 16.854562441606976 30.60417315478044 28.533167237620678 24.008097165991906 38-39 21.45437558393024 23.337485726149694 27.410983078999273 27.797155610920793 40-41 19.51209384407765 25.44793937506488 31.125298453233675 23.914668327623794 42-43 25.14377660126648 23.862763417419288 32.40112114606042 18.592338835253816 44-45 19.550503477628983 24.412955465587043 28.020346724800167 28.016194331983808 46-47 25.685663863801516 20.648811377556317 27.87916536904391 25.786359389598257 48-49 21.96096750752621 20.365410567839717 29.793418457386068 27.880203467248 50-51 17.024810547077752 20.4827156649019 33.559638741824976 28.932835046195372 52-53 20.821135679435272 20.48790615592235 36.77670507630022 21.914253088342157 54-55 18.469843247171184 20.516972905636873 34.04650679954324 26.96667704764871 56-57 23.900134952766532 20.61247794041316 31.449185092909786 24.038202013910514 58-59 18.90169210007267 31.275822692826743 30.418353576248315 19.404131630852277 60-61 19.273331257136924 39.76123741305928 22.755112633655145 18.210318696148654 62-63 15.210214886328247 44.95691892453026 21.26232741617357 18.57053877296792 64-65 15.482196615799854 45.65036852486245 20.410048790615594 18.4573860687221 66-67 15.681511470985154 44.573860687221014 20.56991591404547 19.174711927748366 68-69 15.23201494861414 41.69002387625869 21.256098826949028 21.821862348178136 70-71 16.13619848437662 38.83421571680681 21.403508771929825 23.626077026886744 72-73 16.20367486764248 35.77494030935326 22.061663033322954 25.959721789681307 74-75 16.62514273850306 35.501920481677566 21.696252465483234 26.176684314336136 76 16.71960967507526 35.608844596698844 21.397280182705284 26.27426554552061 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 4.0 19 4.0 20 3.0 21 4.0 22 6.5 23 25.0 24 41.5 25 42.0 26 72.5 27 126.0 28 174.0 29 199.0 30 253.5 31 348.5 32 507.5 33 626.0 34 708.5 35 910.0 36 1092.5 37 1156.0 38 1347.5 39 1716.0 40 2100.5 41 2665.0 42 3022.0 43 3614.0 44 4940.0 45 5662.5 46 5651.0 47 5193.0 48 4191.0 49 3233.0 50 2819.0 51 2407.0 52 1728.0 53 1363.5 54 1266.0 55 1153.5 56 887.5 57 631.0 58 528.0 59 427.0 60 264.5 61 164.5 62 126.0 63 92.0 64 42.0 65 27.0 66 20.5 67 13.0 68 8.5 69 3.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72480016609571 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6198622528327 32.996989515208135 2 13.712508331481892 12.814284231288282 3 6.269717840479894 8.788539395826845 4 3.1681848478115975 5.92131215612997 5 1.901799600088869 4.443060313505658 6 1.3063763608087091 3.662410464029897 7 0.9197956009775606 3.0084085954531297 8 0.4354587869362364 1.6277379840132877 9 0.4132415018884692 1.737776393646839 >10 1.1641857365029993 7.831412851655767 >50 0.03999111308598089 1.2311844700508667 >100 0.031104199066874026 2.1717014429565036 >500 0.004443457009553432 1.0547077753555485 >1k 0.013330371028660299 12.710474410879268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 3411 7.081905948302709 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 1549 3.216028236271151 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 1162 2.4125402263054085 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 508 1.0547077753555485 No Hit TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 277 0.5751064050659193 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 163 0.33842001453337484 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 158 0.3280390324924738 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT 123 0.25537215820616627 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT 110 0.22838160489982354 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT 109 0.22630540849164332 No Hit CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 106 0.22007681926710268 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 82 0.1702481054707775 No Hit CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 80 0.1660957126544171 No Hit TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 68 0.14118135575625454 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 66 0.1370289629398941 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT 65 0.1349527665317139 No Hit CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 60 0.12457178449081284 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT 60 0.12457178449081284 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 60 0.12457178449081284 No Hit CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 52 0.10796221322537111 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006228589224540642 0.0 2 0.0 0.0 0.0 0.006228589224540642 0.0 3 0.0 0.0 0.0 0.006228589224540642 0.0 4 0.0 0.0 0.0 0.006228589224540642 0.0 5 0.0 0.0 0.0 0.006228589224540642 0.0 6 0.0 0.0 0.0 0.006228589224540642 0.0 7 0.0 0.0 0.0 0.006228589224540642 0.0 8 0.0 0.0 0.0 0.006228589224540642 0.0 9 0.0 0.0 0.0 0.008304785632720855 0.0 10 0.0 0.0 0.0 0.01038098204090107 0.0 11 0.0 0.0 0.0 0.01038098204090107 0.0 12 0.0 0.0 0.0 0.01038098204090107 0.0 13 0.0 0.0 0.0 0.01038098204090107 0.0 14 0.0 0.0 0.0 0.01038098204090107 0.0 15 0.0 0.0 0.0 0.012457178449081284 0.0 16 0.0 0.0 0.0 0.014533374857261496 0.0 17 0.0 0.0 0.0 0.022838160489982353 0.0 18 0.0 0.0 0.0 0.022838160489982353 0.0 19 0.0 0.0 0.0 0.029066749714522992 0.0 20 0.0 0.0 0.0 0.031142946122703206 0.0 21 0.0 0.0 0.0 0.035295338939063635 0.0 22 0.0 0.0 0.0 0.03737153534724385 0.0 23 0.0 0.0 0.0 0.03944773175542406 0.0 24 0.0 0.0 0.0 0.03944773175542406 0.0 25 0.0 0.0 0.0 0.03944773175542406 0.0 26 0.0 0.0 0.0 0.03944773175542406 0.0 27 0.0 0.0 0.0 0.045676320979964706 0.0 28 0.0 0.0 0.0 0.05398110661268556 0.0 29 0.0 0.0 0.0 0.06228589224540641 0.0 30 0.0 0.0 0.0 0.07266687428630748 0.0 31 0.0 0.0 0.0 0.09135264195992941 0.0 32 0.0 0.0 0.0 0.12664798089899304 0.0 33 0.0 0.0 0.0 0.14118135575625454 0.0 34 0.0 0.0 0.0 0.1806290875116786 0.0 35 0.0 0.0 0.0 0.23045780130800372 0.0 36 0.0 0.0 0.0 0.28859130073704975 0.0 37 0.0 0.0 0.0 0.38202013910515936 0.0 38 0.0 0.0 0.0 0.5668016194331984 0.0 39 0.0 0.0 0.0 0.7328973320876155 0.0 40 0.0 0.0 0.0 0.9218312052320149 0.0 41 0.0 0.0 0.0 1.1958891311118032 0.0 42 0.0 0.0 0.0 1.4242707360116267 0.0 43 0.0 0.0 0.0 1.7128620367486764 0.0 44 0.0 0.0 0.0 1.9931485518530052 0.0 45 0.0 0.0 0.0 2.298349423855497 0.0 46 0.0 0.0 0.0 2.5661787605107444 0.0 47 0.0 0.0 0.0 2.8568462576559743 0.0 48 0.0 0.0 0.0 3.2305616111284126 0.0 49 0.0 0.0 0.0 3.5502958579881656 0.0 50 0.0 0.0 0.0 3.8202013910515933 0.0 51 0.0 0.0 0.0 4.166926191217689 0.0 52 0.0 0.0 0.0 4.492889027301983 0.0 53 0.0 0.0 0.0 4.868680577182602 0.0 54 0.0 0.0 0.0 5.161424270736012 0.0 55 0.0 0.0 0.0 5.514377660126648 0.0 56 0.0 0.0 0.0 5.7448354614346515 0.0 57 0.0 0.0 0.0 6.029274369355341 0.0 58 0.0 0.0 0.0 6.280494134745147 0.0 59 0.0 0.0 0.0 6.569085435482196 0.0 60 0.0 0.0 0.0 6.832762379321084 0.0 61 0.0 0.0 0.0 7.171182393854458 0.0 62 0.0 0.0 0.0 7.4867642478978516 0.0 63 0.0 0.0 0.0 7.821031869614866 0.0 64 0.0 0.0 0.0 8.124156545209177 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGTC 15 0.0022119458 70.0 57 CTATGCG 15 0.0022119458 70.0 41 ACCGGCG 15 0.0022119458 70.0 55 TAAATGG 15 0.0022119458 70.0 17 CACCGGC 15 0.0022119458 70.0 54 GCGTCAA 15 0.0022119458 70.0 59 AAAATGT 15 0.0022119458 70.0 67 CGTCAAA 15 0.0022119458 70.0 60 CCACCGG 15 0.0022119458 70.0 53 TTTACAC 215 0.0 63.488373 2 CTATACA 115 0.0 60.869564 1 CTTTACA 225 0.0 60.66666 1 TTACACA 235 0.0 58.085106 3 CTTTATA 25 2.3680243E-4 55.999996 2 GAACAAG 25 2.3680243E-4 55.999996 25 CGGACAC 20 0.0069118557 52.5 28 CATGGTT 20 0.0069118557 52.5 14 TCACATC 20 0.0069118557 52.5 5 TAGACAC 20 0.0069118557 52.5 5 GCTATGC 20 0.0069118557 52.5 40 >>END_MODULE