FastQCFastQC Report
Wed 25 May 2016
SRR1780395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479380
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT301.2993076E-436.66981
GATACAC301.3089199E-436.6238983
CATCGTT353.2271765E-431.40829532
CTATACA1350.030.9656071
ACTGACG300.005730411529.34503411
GGAATCC300.005736266329.33890539
TATCCAC554.998021E-627.9673443
CACGTCG757.016388E-826.39123330
CGGTGGT1600.026.09997443
CTTATAA1051.10958354E-1025.1450061
TCGTTGT450.00139458824.45164134
GTAACCA450.00140224524.42867533
CAGTCAG551.5750233E-424.03719715
TTTAACG803.5987923E-622.00647734
GCCACGT803.618412E-621.99269528
ACGTCGT602.8797894E-421.988131
TGATACA500.00259257321.9789262
ATTAGAG1057.817471E-820.9279443
TAACGGC856.090715E-620.70981636
ACATGTT856.1183073E-620.69900529
Produced by FastQC (version 0.11.5)