FastQCFastQC Report
Wed 25 May 2016
SRR1780395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479380
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT328426.850932454420293RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT307506.414535441612082No Hit
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC99542.0764320580750133No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC56361.1756852601276648No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG36000.7509700029204389No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT30060.6270599524385665No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC16900.35253869581542824No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG15960.33293003462806126No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC14450.30143101506112063No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC9470.19754683132379322No Hit
CTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC9320.19441778964495807No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG7230.1508198089198548No Hit
CTTATTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG7010.1462305477908966No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC6830.14247569777629437No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG5580.11640035045266803No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC5350.11160248654512078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAA207.855001E-443.99645218
GTAAGCG207.855001E-443.9964523
TAAGCGC207.855001E-443.9964524
CCGTCTT33850.042.0512244
TTTACAC11600.040.3932952
CTTTACA12400.037.8227841
GCCGTCT38200.037.2626643
CTATACA7250.037.052491
AAGTACC250.002348701535.20818311
TAAGTCA250.002348701535.20818310
CAAATGT250.002348701535.20818315
GTATCTC250.002352310835.19716332
GGACAGT250.002352310835.19716317
CCACGTA250.002352310835.19716317
TCTTTAT2550.032.8124771
TTACACA14950.031.4891033
CAATCCA353.2165082E-431.42603720
CCGTGCG502.357092E-630.8007329
CTTATAC43450.030.7099021
TTATACA44200.030.0111542
Produced by FastQC (version 0.11.5)