FastQCFastQC Report
Wed 25 May 2016
SRR1780394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21725
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCG150.00219581169.99769619
TAGGAAA150.00219581169.99769629
AGGAAAT150.00219581169.99769630
CCATAGT150.00219581169.99769620
TTTACAC200.006861828352.4982722
GCAACCT305.753054E-446.6651328
CGAGGCA305.753054E-446.6651324
ACGAGGC305.753054E-446.6651323
TAACCAA450.004217531531.11008510
GACGAGG450.004217531531.11008522
GGCAACC500.007059113627.99907927
CACCCTA500.007059113627.9990799
CGACGAG500.007059113627.99907921
AGGCAAC500.007059113627.99907926
GACGCTG905.8196856E-627.22132513
ACGCTGC1056.279188E-726.66578914
TGCCGAC700.001188424324.99917618
TCTGACG1001.3187404E-524.49919310
GCTGCCG750.001776329823.33256516
CTGCCGA750.001776329823.33256517
Produced by FastQC (version 0.11.5)