##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21725 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.074430379746836 27.0 12.0 34.0 12.0 34.0 2 24.347940161104717 27.0 12.0 34.0 11.0 34.0 3 24.330034522439586 31.0 11.0 34.0 11.0 34.0 4 24.51567318757192 31.0 11.0 34.0 11.0 34.0 5 24.96349827387802 31.0 11.0 34.0 11.0 34.0 6 27.44234752589183 34.0 11.0 38.0 11.0 38.0 7 27.640506329113926 34.0 21.0 38.0 11.0 38.0 8 27.56998849252014 33.0 21.0 37.0 11.0 38.0 9 27.688699654775604 34.0 12.0 38.0 12.0 38.0 10-11 27.154890678941314 33.0 11.0 38.0 11.0 38.0 12-13 27.230817031070195 33.0 11.0 38.0 11.0 38.0 14-15 26.909436133486768 31.5 11.0 38.0 11.0 38.0 16-17 26.56897583429229 31.0 11.0 38.0 11.0 38.0 18-19 26.735166858457998 31.0 11.0 38.0 11.0 38.0 20-21 26.88826237054085 31.0 11.0 38.0 11.0 38.0 22-23 26.472359033371692 31.0 11.0 38.0 11.0 38.0 24-25 26.672589182968927 31.0 11.0 38.0 11.0 38.0 26-27 26.508538550057537 31.0 11.0 38.0 11.0 38.0 28-29 26.304533947065593 31.0 11.0 38.0 11.0 38.0 30-31 26.259700805523593 31.0 11.0 38.0 11.0 38.0 32-33 26.31452243958573 31.0 11.0 38.0 11.0 38.0 34-35 26.368952819332566 31.0 11.0 38.0 11.0 38.0 36-37 26.295535097813577 31.0 11.0 38.0 11.0 38.0 38-39 26.1228998849252 29.0 11.0 38.0 11.0 38.0 40-41 26.158158803222094 27.0 11.0 38.0 11.0 38.0 42-43 26.43164556962025 31.0 11.0 38.0 11.0 38.0 44-45 25.93993095512083 28.5 11.0 37.0 11.0 38.0 46-47 26.362416570771003 31.0 11.0 37.5 11.0 38.0 48-49 26.43065592635213 31.0 11.0 38.0 11.0 38.0 50-51 26.359171461449943 31.0 11.0 37.0 11.0 38.0 52-53 26.317882623705408 31.0 11.0 37.0 11.0 38.0 54-55 26.185500575373993 30.0 11.0 37.0 11.0 38.0 56-57 25.97512082853855 27.0 11.0 37.0 11.0 38.0 58-59 25.96991944764097 27.5 11.0 37.0 11.0 38.0 60-61 26.053555811277327 27.0 11.0 37.0 11.0 38.0 62-63 25.903774453394707 27.0 11.0 37.0 11.0 38.0 64-65 26.031001150747986 29.0 11.0 37.0 11.0 38.0 66-67 26.1492750287687 30.0 11.0 37.0 11.0 38.0 68-69 25.637008055235903 27.5 11.0 37.0 11.0 38.0 70-71 25.835742232451096 27.5 11.0 37.0 11.0 38.0 72-73 26.045132336018412 29.0 11.0 37.0 11.0 38.0 74-75 25.779654775604143 27.5 11.0 37.0 11.0 38.0 76 25.149781357882624 25.0 11.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5455745701577186 1101 2 -1.0138475992743494 1101 3 -0.7935553038817673 1101 4 1.0898790062102073 1101 5 -1.632844464943247 1101 6 -1.3322099288472131 1101 7 -2.0758500899020227 1101 8 -0.19130696600563724 1101 9 0.46683126757317694 1101 10-11 -1.6476922917525556 1101 12-13 -1.967218339278027 1101 14-15 0.8419805096045998 1101 16-17 -0.7855647907692429 1101 18-19 -0.8423985756539345 1101 20-21 -1.9294725230084033 1101 22-23 -1.7561770577048108 1101 24-25 0.03771918617428227 1101 26-27 -0.9715765978761262 1101 28-29 -0.4662956812387016 1101 30-31 -0.8350501878002561 1101 32-33 -0.4410595603008254 1101 34-35 0.10447836108343367 1101 36-37 -0.8697354848532868 1101 38-39 -0.11124106948875578 1101 40-41 -0.6746317923406338 1101 42-43 -1.8817565068566218 1101 44-45 -0.4745134728975664 1101 46-47 -0.9304289438543627 1101 48-49 -1.869279472666932 1101 50-51 -0.5585566591935063 1101 52-53 -0.3937622937554437 1101 54-55 -1.3662394221113274 1101 56-57 -1.5429635468464724 1101 58-59 -0.705383420922189 1101 60-61 -1.7888065481596875 1101 62-63 -0.46054200916812604 1101 64-65 -0.9794533494867039 1101 66-67 -1.055444683876864 1101 68-69 -0.8383700795838465 1101 70-71 -1.234487890167287 1101 72-73 -1.0413391598759212 1101 74-75 -0.8147816055158756 1101 76 -0.05243623811920273 1102 1 0.8800883086301603 1102 2 0.21910694618019733 1102 3 0.5601378779364161 1102 4 0.3829661274223284 1102 5 0.7838222017234209 1102 6 0.2151385560012713 1102 7 0.11543778888585621 1102 8 1.6060415188428472 1102 9 1.925164600906509 1102 10-11 2.9683114961262334 1102 12-13 0.7527153728431841 1102 14-15 0.11494452475611538 1102 16-17 -0.7848545634965163 1102 18-19 0.7927813485884876 1102 20-21 0.36195740123401876 1102 22-23 1.5174971847194314 1102 24-25 0.5301434285985245 1102 26-27 -1.5840292493912749 1102 28-29 0.21362856118554063 1102 30-31 -0.07108238476995155 1102 32-33 -3.3451788784826455 1102 34-35 -0.1405500480074764 1102 36-37 0.809715272722471 1102 38-39 0.5038157486930643 1102 40-41 0.5883890409926984 1102 42-43 0.5983571295070149 1102 44-45 -2.227354381988473 1102 46-47 0.6675824197820006 1102 48-49 1.4223871939997323 1102 50-51 -3.063291507678354 1102 52-53 -0.396366460422108 1102 54-55 1.2606545172826102 1102 56-57 0.3893281198201919 1102 58-59 0.7368514275626588 1102 60-61 0.39395860335546473 1102 62-63 -0.7377673879560014 1102 64-65 -0.4188472888806416 1102 66-67 0.07594736157768267 1102 68-69 -3.841921215947483 1102 70-71 -0.5410693295612248 1102 72-73 1.1146740976998366 1102 74-75 -2.88769827218254 1102 76 -1.816167298725265 1103 1 -0.9075274321105802 1103 2 -0.6535782390049896 1103 3 -0.6981568751500049 1103 4 -1.6037236873924847 1103 5 -1.064943230375345 1103 6 -0.05106514770243109 1103 7 -0.6600251740771057 1103 8 -0.0891745305398679 1103 9 -0.3418107077354655 1103 10-11 -0.881418442145371 1103 12-13 -0.8939860160457052 1103 14-15 -0.5471889628982076 1103 16-17 -0.6570574955952821 1103 18-19 -1.347168497090525 1103 20-21 -1.7737486172845003 1103 22-23 -0.6989380004657519 1103 24-25 -1.0531899047348077 1103 26-27 -1.7774127370455979 1103 28-29 0.06625201797566405 1103 30-31 -0.8067112427946412 1103 32-33 -3.364179650087582 1103 34-35 -0.9527606961556252 1103 36-37 -0.794258492709627 1103 38-39 -0.4072567821711317 1103 40-41 -0.5542614219702635 1103 42-43 -1.2546521297522446 1103 44-45 -2.553357468408226 1103 46-47 -1.0822246789834296 1103 48-49 -1.0081683615558248 1103 50-51 -0.3144489150857588 1103 52-53 -1.23326846659495 1103 54-55 -1.8496849888902283 1103 56-57 -0.6941981147477065 1103 58-59 -2.069861535400303 1103 60-61 0.1713505786641072 1103 62-63 -1.7806879126473625 1103 64-65 -0.0669954370287904 1103 66-67 0.4529728245406446 1103 68-69 -2.422264580145015 1103 70-71 -1.1707954098081395 1103 72-73 -0.830059698596461 1103 74-75 -0.6295964203306852 1103 76 0.07214288645992184 1104 1 1.086377865592187 1104 2 0.7214803639017155 1104 3 1.3376299665440108 1104 4 1.4009645451438466 1104 5 2.2743285308373444 1104 6 1.8551702015708926 1104 7 1.3915453838225638 1104 8 0.8125288606150001 1104 9 1.2621899173622069 1104 10-11 -0.5338641367851586 1104 12-13 0.406295277906473 1104 14-15 0.5501699994396567 1104 16-17 1.1189231263768988 1104 18-19 1.0850084055505143 1104 20-21 1.0675547113606036 1104 22-23 1.1761206024409496 1104 24-25 0.94470039062384 1104 26-27 1.136401921494798 1104 28-29 2.244482991565288 1104 30-31 1.382537077255364 1104 32-33 0.0129105202515305 1104 34-35 1.7080456798406232 1104 36-37 2.0112579942414612 1104 38-39 1.8556749259082537 1104 40-41 0.8707268574483926 1104 42-43 0.31473371178549314 1104 44-45 -1.11461704021632 1104 46-47 1.518650457756685 1104 48-49 0.7182732699491012 1104 50-51 0.9284015302963304 1104 52-53 0.6484317990715631 1104 54-55 1.3737874286750156 1104 56-57 0.031930967921457665 1104 58-59 1.0513292756639245 1104 60-61 1.6032149324693883 1104 62-63 2.3473789728034937 1104 64-65 1.4798868883345477 1104 66-67 1.4710225198055298 1104 68-69 -0.547518526074068 1104 70-71 -0.21502107006755367 1104 72-73 0.9029454584593317 1104 74-75 1.0248808417415134 1104 76 1.6337931443127083 1105 1 -0.47166474015032733 1105 2 0.16575328764361075 1105 3 -0.8312493171855344 1105 4 -0.2401283847727953 1105 5 0.2899197626990322 1105 6 1.1526385560012713 1105 7 -0.9409646501385325 1105 8 0.4642732261599214 1105 9 -1.3705671064105651 1105 10-11 0.6036011302725761 1105 12-13 -0.37704834666901377 1105 14-15 0.765668610121967 1105 16-17 -0.18634084398431838 1105 18-19 0.2952584827348268 1105 20-21 0.6837942914779198 1105 22-23 0.602100843256018 1105 24-25 0.7435580627448672 1105 26-27 0.6391033725599442 1105 28-29 0.574909048990417 1105 30-31 -0.1600686652577572 1105 32-33 -0.8872901589704512 1105 34-35 0.7380703788217922 1105 36-37 0.3512549068688102 1105 38-39 1.347184651132089 1105 40-41 1.0794332483097726 1105 42-43 1.2058266417021386 1105 44-45 0.7078590326456755 1105 46-47 0.6405244929527321 1105 48-49 -0.09285670843929239 1105 50-51 -0.13265126377591585 1105 52-53 1.5554246981144786 1105 54-55 0.40242280996553603 1105 56-57 -0.7543456606676138 1105 58-59 0.44188191536753507 1105 60-61 1.5639281155505884 1105 62-63 0.1545725510683873 1105 64-65 0.8921283208754573 1105 66-67 0.19122937377280635 1105 68-69 0.2142906742964179 1105 70-71 0.16758158507292364 1105 72-73 -1.0093731583977252 1105 74-75 -0.5485214429142466 1105 76 -0.8447496157984347 1106 1 -0.21090433842866219 1106 2 -1.7919224655845092 1106 3 -1.0235018279459354 1106 4 0.19638524506938637 1106 5 0.5485280840763629 1106 6 -1.4355967381163772 1106 7 -1.2117680934670858 1106 8 0.3670709306075537 1106 9 -1.0527765755640779 1106 10-11 0.8460688490674109 1106 12-13 -0.10803830362740285 1106 14-15 -0.5105150340674136 1106 16-17 -0.12446853408475178 1106 18-19 -0.3078620337644544 1106 20-21 -1.0567006870012747 1106 22-23 -1.587282227045275 1106 24-25 0.08161401683382152 1106 26-27 -0.11573880821480387 1106 28-29 -1.8488714388144594 1106 30-31 -0.0026081200640675206 1106 32-33 -0.11953549612970349 1106 34-35 -0.5325537244780634 1106 36-37 0.248134390369529 1106 38-39 -0.35188278071870016 1106 40-41 0.2395838939338759 1106 42-43 -1.08362816461063 1106 44-45 -0.4056632055178859 1106 46-47 -0.5373808155121154 1106 48-49 -0.4820245707061517 1106 50-51 -0.3261591547371765 1106 52-53 0.2631004513425985 1106 54-55 -0.8018454827173898 1106 56-57 0.6972325315848984 1106 58-59 -1.3642515136138123 1106 60-61 -0.8137619848798323 1106 62-63 0.07243481792635365 1106 64-65 -0.8673767006453481 1106 66-67 -1.0614607266576108 1106 68-69 -1.180616068888657 1106 70-71 0.024647582203481733 1106 72-73 -0.8425869317119279 1106 74-75 -1.113801213359011 1106 76 -3.3042187693135006 1107 1 0.8462003216171716 1107 2 1.518619933193186 1107 3 0.16748365715719515 1107 4 0.3149872313184332 1107 5 0.30654947445069425 1107 6 0.47082037418308786 1107 7 0.34189882784689374 1107 8 -0.5116532863519581 1107 9 -0.3361990354571276 1107 10-11 0.3317449376846753 1107 12-13 0.8000976780379894 1107 14-15 0.08765150527559484 1107 16-17 -0.4284219336263888 1107 18-19 0.7214542381988771 1107 20-21 -0.6967885403244232 1107 22-23 0.27845497692722176 1107 24-25 -1.3821942337391384 1107 26-27 -0.4737411000406233 1107 28-29 -0.6004948154378376 1107 30-31 -1.2300718328218991 1107 32-33 0.29920017995891257 1107 34-35 0.48759524420031397 1107 36-37 -0.3296921948099971 1107 38-39 0.443953735706053 1107 40-41 1.0710392033303613 1107 42-43 0.5305811554810411 1107 44-45 0.21704496866087908 1107 46-47 0.8974931340677159 1107 48-49 1.4499845965971332 1107 50-51 1.7033481027112565 1107 52-53 0.9135970136038658 1107 54-55 0.47899867312676747 1107 56-57 0.43214467826174996 1107 58-59 0.5359585704198047 1107 60-61 -0.21521347456661388 1107 62-63 -0.12829092691704247 1107 64-65 -0.9318343018676529 1107 66-67 0.3406592122270311 1107 68-69 1.1778636866499177 1107 70-71 0.2968973912179962 1107 72-73 0.9207820522452899 1107 74-75 0.758811468077198 1107 76 -0.2818734675564336 1108 1 0.40654664196349444 1108 2 -0.8417263871531375 1108 3 0.3082628779364178 1108 4 -0.08224220591100462 1108 5 0.9404888683900872 1108 6 0.332638556001271 1108 7 0.26293778888585706 1108 8 0.8935415188428486 1108 9 0.8334979342398441 1108 10-11 0.7101864961262336 1108 12-13 -0.5517637938234827 1108 14-15 -0.1328679752438866 1108 16-17 0.4950412698368183 1108 18-19 -1.5281561514115118 1108 20-21 -0.4018967654326495 1108 22-23 -0.9537528152805663 1108 24-25 -0.786523238068142 1108 26-27 0.9969082506087226 1108 28-29 0.2427952278522092 1108 30-31 -0.6753532181032824 1108 32-33 -0.6659080451493118 1108 34-35 -0.944612548007477 1108 36-37 -1.0154930606108614 1108 38-39 0.1769407486930632 1108 40-41 0.548701540992699 1108 42-43 -0.19539287049298792 1108 44-45 -0.38027104865513905 1108 46-47 -0.5367925802179982 1108 48-49 -0.5392794726669337 1108 50-51 -0.04037484101168687 1108 52-53 -0.8337622937554414 1108 54-55 -0.010178816050725459 1108 56-57 -0.5062968801798107 1108 58-59 -1.4868985724373402 1108 60-61 -1.0906247299778684 1108 62-63 -0.6493298879560037 1108 64-65 -0.6988472888806427 1108 66-67 0.8433431949110144 1108 68-69 -0.098067049280818 1108 70-71 -0.03721516289455806 1108 72-73 0.6862365976998355 1108 74-75 -0.10811493884920509 1108 76 0.5002910346080682 1109 1 -1.2537432131089687 1109 2 -2.410422039327049 1109 3 -1.0465197307592362 1109 4 -2.9918074233023084 1109 5 -2.4444386678417978 1109 6 -1.8111295599407562 1109 7 -1.3782216314039992 1109 8 -2.315154133331067 1109 9 -2.33751655851378 1109 10-11 -2.0735816198157977 1109 12-13 -1.7369811851278314 1109 14-15 -1.6030129027801188 1109 16-17 -1.6090167011776728 1109 18-19 -1.6826489050347 1109 20-21 -1.5016069103601843 1109 22-23 -1.5934629602081039 1109 24-25 -1.53869715111162 1109 26-27 -2.7172946479420013 1109 28-29 -2.2456105692492407 1109 30-31 -2.455932928248213 1109 32-33 -2.592574711815981 1109 34-35 -2.1886705190219686 1109 36-37 -2.811435089596369 1109 38-39 -2.731465048408385 1109 40-41 -2.1999941111812156 1109 42-43 -2.749016058898782 1109 44-45 -1.2205609037276055 1109 46-47 -0.6936041744208978 1109 48-49 -1.2497142552756273 1109 50-51 -1.7258820873884986 1109 52-53 -1.6546318589728344 1109 54-55 -1.205830989963765 1109 56-57 -1.7132534019189407 1109 58-59 -1.940231905770677 1109 60-61 -1.289465309688012 1109 62-63 -1.9009240908545522 1109 64-65 -2.19420960772122 1109 66-67 -1.2636133268281142 1109 68-69 -1.9928496579764676 1109 70-71 -2.518084728111951 1109 72-73 -1.8888358660682805 1109 74-75 -2.691593199718774 1109 76 -2.228984327710773 1110 1 -1.2979342323534446 1110 2 -2.1077373161148856 1110 3 -2.2346879417357144 1110 4 -3.1167777250366875 1110 5 -1.0809318966372352 1110 6 -2.806377837441353 1110 7 -0.23804581767151944 1110 8 -1.4441633991899394 1110 9 -2.699835399093491 1110 10-11 -1.6344036678081935 1110 12-13 -2.480506963222389 1110 14-15 -1.7863105981947065 1110 16-17 -1.7304232110374969 1110 18-19 -1.9222545120672514 1110 20-21 -1.2060497708971276 1110 22-23 -1.9809659300346638 1110 24-25 -2.2772336205818036 1110 26-27 -1.0165343723420968 1110 28-29 -2.8058386519292142 1110 30-31 -0.9869379175568369 1110 32-33 -1.7428479358596967 1110 34-35 -2.099694515220591 1110 36-37 -2.1572416944906436 1110 38-39 -3.608633021798738 1110 40-41 -2.804413213105665 1110 42-43 -2.0445731983618387 1110 44-45 -1.7237683164147022 1110 46-47 -2.1446614326770153 1110 48-49 -2.1903177240330542 1110 50-51 -1.2267136388258955 1110 52-53 -1.4784617473073567 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-0.7532333816655594 2116 68-69 0.3428338516200853 2116 70-71 -1.6536115592909546 2116 72-73 -2.141871510408272 2116 74-75 -2.5219888127230803 2116 76 -1.8968260825090475 2117 1 2.6210920965089493 2117 2 1.5013039158771662 2117 3 0.5700810597545996 2117 4 0.12018203651323844 2117 5 0.6095797774809952 2117 6 0.8344567378194547 2117 7 2.6665741525222195 2117 8 -1.9185796932783639 2117 9 1.9062252069671146 2117 10-11 -0.38254077660104 2117 12-13 0.8888422667825786 2117 14-15 0.6677380853621777 2117 16-17 2.138677633473179 2117 18-19 0.9076014243460655 2117 20-21 2.169012325476441 2117 22-23 1.213519911992158 2117 24-25 1.583173731628829 2117 26-27 2.089029462729936 2117 28-29 1.7306740157309939 2117 30-31 1.3846467818967128 2117 32-33 1.34681922757796 2117 34-35 0.680235936841008 2117 36-37 1.3348099696921665 2117 38-39 1.9114862032385176 2117 40-41 1.348095480386636 2117 42-43 2.9440010689009526 2117 44-45 2.4573047089206206 2117 46-47 1.955934692509274 2117 48-49 1.8655690121815525 2117 50-51 0.4262918256549817 2117 52-53 1.4092680092748608 2117 54-55 1.9140636081917002 2117 56-57 1.0706728167898873 2117 58-59 2.211283245744479 2117 60-61 2.7263449669918316 2117 62-63 1.4409731423470298 2117 64-65 2.005395135361784 2117 66-67 2.2021310736988937 2117 68-69 4.775266284052517 2117 70-71 2.7276333219539275 2117 72-73 2.1480547795180165 2117 74-75 2.4124911217568545 2117 76 5.371806186123219 2118 1 -0.5785513972521912 2118 2 -2.409569524408038 2118 3 -1.9058547691224064 2118 4 -0.49479122551884913 2118 5 -1.6426483865118726 2118 6 -0.5532437969399062 2118 7 2.038231906532914 2118 8 -0.3829290693924463 2118 9 -0.5233648108581974 2118 10-11 -2.198048797991415 2118 12-13 -0.5198030095097579 2118 14-15 -0.4811032693615296 2118 16-17 -1.2347626517318098 2118 18-19 -2.6828620337644544 2118 20-21 -0.7699359811189233 2118 22-23 -2.8372822270452716 2118 24-25 -1.9404448066955915 2118 26-27 -2.5642682199795104 2118 28-29 -1.8562243799909304 2118 30-31 -3.0320198847699515 2118 32-33 -0.832770790247352 2118 34-35 -2.4222596068310054 2118 36-37 -1.568042080218703 2118 38-39 -2.984235721895171 2118 40-41 -2.8707102237131856 2118 42-43 -1.5542163999047496 2118 44-45 -2.015957323164944 2118 46-47 -1.007969050806235 2118 48-49 -1.9232010412943836 2118 50-51 -3.333512095913651 2118 52-53 -1.339840725127992 2118 54-55 -3.2944925415409188 2118 56-57 -1.6262968801798081 2118 58-59 -2.2539573959667543 2118 60-61 -2.4461149260562998 2118 62-63 -0.8834475350148239 2118 64-65 -2.0070825829982866 2118 66-67 -2.083519550187024 2118 68-69 -2.842380774771012 2118 70-71 -2.5268230060318118 2118 72-73 -1.9455281081825184 2118 74-75 -0.5990953310060689 2118 76 -1.4071599457840875 2119 1 -0.9816015061846528 2119 2 1.827903242476495 2119 3 1.3882628779364161 2119 4 -0.15927924294804185 2119 5 -0.26247409457287674 2119 6 0.4674533708160844 2119 7 -2.030395544447476 2119 8 0.10835633365766029 2119 9 2.6334979342398412 2119 10-11 -0.03573942979969047 2119 12-13 -0.11620823826792659 2119 14-15 2.199724617348707 2119 16-17 -2.0397735449779972 2119 18-19 -0.6311191143744743 2119 20-21 0.7262513827154997 2119 22-23 -0.3100491115768662 2119 24-25 2.2060693545244483 2119 26-27 0.6361675098679846 2119 28-29 -0.7609084758514975 2119 30-31 -0.37461247736254677 2119 32-33 0.37038825114698426 2119 34-35 -0.9342421776371062 2119 36-37 -2.1029004680182695 2119 38-39 -0.5986148068624928 2119 40-41 0.39018302247417935 2119 42-43 0.22682935172923635 2119 44-45 -0.2817525301366217 2119 46-47 -0.594570357995778 2119 48-49 0.5659057125182514 2119 50-51 -1.444078544715392 2119 52-53 -0.11598451597766513 2119 54-55 2.1935248876529805 2119 56-57 0.4662957124127871 2119 58-59 -0.75504672058549 2119 60-61 -0.026180285533424552 2119 62-63 -0.4041447027708145 2119 64-65 -1.5484769185102714 2119 66-67 -2.1951753236075007 2119 68-69 1.608599617385849 2119 70-71 -0.8498077554871486 2119 72-73 -0.7559856245223848 2119 74-75 -1.2962630869973566 2119 76 -1.3352645209474865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 39.0 13 241.0 14 644.0 15 1050.0 16 1373.0 17 1563.0 18 1663.0 19 1380.0 20 1210.0 21 963.0 22 677.0 23 447.0 24 355.0 25 265.0 26 229.0 27 208.0 28 208.0 29 229.0 30 233.0 31 320.0 32 361.0 33 511.0 34 726.0 35 1232.0 36 2295.0 37 3302.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.865833911635438 39.685403654869305 8.290538977561878 32.158223455933374 2 17.82278481012658 27.30494821634062 30.34752589182969 24.52474108170311 3 19.097813578826237 28.28998849252014 25.905638665132336 26.70655926352129 4 19.43843498273878 23.719217491369392 28.49252013808976 28.349827387802073 5 10.504027617951667 34.918296892980436 32.92980437284235 21.64787111622555 6 15.613348676639816 34.67433831990794 15.065592635212887 34.64672036823935 7 13.919447640966629 42.724971231300344 15.949367088607595 27.40621403912543 8 12.372842347525893 43.438434982738784 16.13808975834292 28.050632911392405 9 14.738780207134639 27.68239355581128 16.21173762945915 41.36708860759494 10-11 14.64211737629459 30.948216340621403 24.699654775604145 29.710011507479862 12-13 13.691599539700805 27.171461449942463 24.711162255466053 34.42577675489068 14-15 14.79631760644419 29.707710011507483 21.18066743383199 34.315304948216344 16-17 18.943613348676642 30.057537399309552 21.521288837744535 29.477560414269277 18-19 16.858457997698505 28.780207134637514 20.892980437284233 33.46835443037975 20-21 15.42922899884925 28.87686996547756 21.31415420023015 34.379746835443036 22-23 19.95397008055236 29.093210586881472 19.965477560414268 30.9873417721519 24-25 17.477560414269274 32.499424626006906 19.203682393555813 30.81933256616801 26-27 15.415420023014958 32.678941311852704 22.204833141542004 29.700805523590333 28-29 18.105868814729575 29.822784810126585 24.3590333716916 27.712313003452245 30-31 17.238204833141545 29.318757192174914 25.14614499424626 28.29689298043728 32-33 15.008055235903337 32.70886075949367 24.025316455696203 28.257767548906788 34-35 15.726121979286537 31.060989643268123 23.703107019562715 29.509781357882623 36-37 15.983889528193327 28.386651323360184 25.965477560414268 29.66398158803222 38-39 15.98849252013809 26.306098964326814 27.8204833141542 29.884925201380895 40-41 14.623705408515535 27.53049482163406 26.020713463751438 31.825086306098964 42-43 12.563866513233602 26.869965477560413 28.667433831990795 31.89873417721519 44-45 12.79861910241657 28.97353279631761 28.48331415420023 29.744533947065595 46-47 13.83199079401611 29.63176064441887 25.78596087456847 30.750287686996547 48-49 13.176064441887227 30.86306098964327 24.036823935558115 31.924050632911396 50-51 13.084004602991945 30.38204833141542 23.385500575373992 33.14844649021864 52-53 12.87686996547756 29.252013808975835 24.087456846950516 33.783659378596084 54-55 12.784810126582277 29.69850402761795 23.24510932105869 34.27157652474108 56-57 14.697353279631761 29.622554660529342 22.527042577675488 33.153049482163404 58-59 14.418872266973532 29.18757192174914 22.962025316455698 33.43153049482163 60-61 13.631760644418872 27.668584579976987 22.766398158803224 35.93325661680092 62-63 13.9102416570771 28.88607594936709 22.329113924050635 34.87456846950518 64-65 14.718066743383199 30.64902186421174 20.04602991944764 34.58688147295742 66-67 13.96547756041427 33.21288837744534 19.065592635212887 33.7560414269275 68-69 15.284234752589182 32.48101265822785 18.522439585730723 33.712313003452245 70-71 15.256616800920597 33.45914844649022 18.533947065592635 32.75028768699655 72-73 13.781357882623704 33.410817031070195 17.700805523590336 35.10701956271576 74-75 13.733026467203683 32.12888377445339 19.29344073647871 34.84464902186421 76 14.039125431530493 29.979286536248562 19.60874568469505 36.37284234752589 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 2.0 14 3.0 15 2.5 16 6.5 17 10.0 18 8.5 19 11.0 20 16.5 21 18.0 22 18.0 23 22.5 24 33.5 25 40.0 26 48.5 27 90.0 28 110.0 29 97.0 30 124.5 31 175.5 32 255.5 33 312.0 34 349.5 35 454.0 36 551.5 37 582.0 38 679.0 39 832.0 40 992.5 41 1123.0 42 1149.0 43 1226.0 44 1414.5 45 1483.0 46 1440.0 47 1462.5 48 1445.0 49 1387.0 50 1369.0 51 1282.5 52 1089.0 53 946.5 54 911.0 55 860.5 56 733.0 57 583.5 58 511.0 59 448.0 60 339.0 61 277.0 62 261.0 63 217.5 64 148.5 65 112.0 66 85.0 67 69.0 68 60.5 69 44.5 70 31.0 71 25.0 72 23.0 73 20.0 74 14.0 75 9.0 76 7.5 77 6.0 78 7.5 79 9.0 80 9.0 81 6.0 82 2.0 83 1.0 84 1.5 85 4.0 86 4.5 87 3.0 88 5.0 89 4.5 90 1.5 91 2.0 92 3.0 93 2.0 94 2.0 95 2.5 96 2.0 97 1.5 98 2.5 99 4.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5063291139240507 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 21725.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.81588032220944 #Duplication Level Percentage of deduplicated Percentage of total 1 91.32508648705316 80.19792865362486 2 5.608554355802495 9.850402761795166 3 1.9289233672292694 5.081703107019562 4 0.6604465876926303 2.3199079401611047 5 0.2463570604885208 1.0817031070195626 6 0.11007443128210503 0.5799769850402762 7 0.047174756263759304 0.2899884925201381 8 0.04193311667889716 0.2945914844649022 9 0.005241639584862145 0.04142692750287687 >10 0.026208197924310724 0.26237054085155354 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.004602991944764097 0.0 13 0.0 0.0 0.0 0.004602991944764097 0.0 14 0.0 0.0 0.0 0.004602991944764097 0.0 15 0.0 0.0 0.0 0.004602991944764097 0.0 16 0.0 0.0 0.0 0.004602991944764097 0.0 17 0.0 0.0 0.0 0.004602991944764097 0.0 18 0.0 0.0 0.0 0.009205983889528193 0.0 19 0.0 0.0 0.0 0.009205983889528193 0.0 20 0.0 0.0 0.0 0.009205983889528193 0.0 21 0.0 0.0 0.0 0.009205983889528193 0.0 22 0.0 0.0 0.0 0.009205983889528193 0.0 23 0.0 0.0 0.0 0.01380897583429229 0.0 24 0.0 0.0 0.0 0.02761795166858458 0.0 25 0.0 0.0 0.0 0.02761795166858458 0.0 26 0.0 0.0 0.0 0.02761795166858458 0.0 27 0.0 0.0 0.0 0.03222094361334868 0.0 28 0.0 0.0 0.0 0.04602991944764097 0.0 29 0.0 0.0 0.0 0.05063291139240506 0.0 30 0.0 0.0 0.0 0.05523590333716916 0.0 31 0.0 0.0 0.0 0.05523590333716916 0.0 32 0.0 0.0 0.0 0.06904487917146145 0.0 33 0.0 0.0 0.0 0.07825086306098965 0.0 34 0.0 0.0 0.0 0.11967779056386652 0.0 35 0.0 0.0 0.0 0.1334867663981588 0.0 36 0.0 0.0 0.0 0.17951668584579977 0.0 37 0.0 0.0 0.0 0.23475258918296893 0.0 38 0.0 0.0 0.0 0.34522439585730724 0.0 39 0.0 0.0 0.0 0.46029919447640966 0.0 40 0.0 0.0 0.0 0.5845799769850403 0.0 41 0.0 0.0 0.0 0.7410817031070196 0.0 42 0.0 0.0 0.0 0.8929804372842347 0.0 43 0.0 0.0 0.0 1.0540851553509782 0.0 44 0.0 0.0 0.0 1.2428078250863062 0.0 45 0.0 0.0 0.0 1.4361334867663982 0.0 46 0.0 0.0 0.0 1.5696202531645569 0.0 47 0.0 0.0 0.0 1.7721518987341771 0.0 48 0.0 0.0 0.0 1.947065592635213 0.0 49 0.0 0.0 0.0 2.103567318757192 0.0 50 0.0 0.0 0.0 2.3014959723820483 0.0 51 0.0 0.0 0.0 2.5454545454545454 0.0 52 0.0 0.0 0.0 2.6881472957422323 0.0 53 0.0 0.0 0.0 2.8722669735327964 0.0 54 0.0 0.0 0.0 3.047180667433832 0.0 55 0.0 0.0 0.0 3.2589182968929804 0.0 56 0.0 0.0 0.0 3.470655926352129 0.0 57 0.0 0.0 0.0 3.6409666283084006 0.0 58 0.0 0.0 0.0 3.7836593785960875 0.0 59 0.0 0.0 0.0 3.9263521288837744 0.0 60 0.0 0.0 0.0 4.142692750287687 0.0 61 0.0 0.0 0.0 4.2945914844649025 0.0 62 0.0 0.0 0.0 4.446490218642118 0.0 63 0.0 0.0 0.0 4.653624856156502 0.0 64 0.0 0.0 0.0 4.805523590333717 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGTCG 15 0.002195811 69.997696 19 TAGGAAA 15 0.002195811 69.997696 29 AGGAAAT 15 0.002195811 69.997696 30 CCATAGT 15 0.002195811 69.997696 20 TTTACAC 20 0.0068618283 52.498272 2 GCAACCT 30 5.753054E-4 46.66513 28 CGAGGCA 30 5.753054E-4 46.66513 24 ACGAGGC 30 5.753054E-4 46.66513 23 TAACCAA 45 0.0042175315 31.110085 10 GACGAGG 45 0.0042175315 31.110085 22 GGCAACC 50 0.0070591136 27.999079 27 CACCCTA 50 0.0070591136 27.999079 9 CGACGAG 50 0.0070591136 27.999079 21 AGGCAAC 50 0.0070591136 27.999079 26 GACGCTG 90 5.8196856E-6 27.221325 13 ACGCTGC 105 6.279188E-7 26.665789 14 TGCCGAC 70 0.0011884243 24.999176 18 TCTGACG 100 1.3187404E-5 24.499193 10 GCTGCCG 75 0.0017763298 23.332565 16 CTGCCGA 75 0.0017763298 23.332565 17 >>END_MODULE