FastQCFastQC Report
Wed 25 May 2016
SRR1780394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21725
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT306614.112773302646719RNA PCR Primer, Index 27 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC12915.942462600690448No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT11435.261219792865362No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC4422.034522439585731No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2411.1093210586881472No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1330.6121979286536249No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1220.5615650172612198No Hit
CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC1180.5431530494821634No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1150.5293440736478712No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC990.4556962025316456No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT920.42347525891829685No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC880.40506329113924056No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT640.2945914844649022No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT640.2945914844649022No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT630.2899884925201381No Hit
CTTATCACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC620.28538550057537404No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG600.2761795166858458No Hit
ATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGTCTT510.2347525891829689TruSeq Adapter, Index 4 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT450.20713463751438435RNA PCR Primer, Index 27 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT400.18411967779056385No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT400.18411967779056385No Hit
TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC390.17951668584579977No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT370.17031070195627157No Hit
ATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGTCTT370.17031070195627157Illumina PCR Primer Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGTCTT360.16570771001150747No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG360.16570771001150747No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGTCTT350.1611047180667434No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGT350.1611047180667434No Hit
CTTATACACATCTCCGAGCCCACGCGACTTGACCCTATCTCGTATGCCGT320.1472957422324511No Hit
ATACACATCTCCGAGCCCACGAGAATTGACCCTATCTCGTATGCCGTCTT310.14269275028768702No Hit
CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC300.1380897583429229No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCCT270.12428078250863062No Hit
TCTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT270.12428078250863062No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTC270.12428078250863062No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC260.11967779056386652No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTC250.11507479861910241No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTC240.11047180667433831No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCAGTCTT240.11047180667433831No Hit
ATACACATCTCCGAGCCCACGAGCCTTGACCCTATCTCGTATGCCGTCTT230.10586881472957421RNA PCR Primer, Index 27 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT220.10126582278481014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG150.002195524870.017
AACCTCC150.002195524870.040
TAACCTC150.002195524870.039
TCTTTAT150.002195524870.01
GCACACG150.002195524870.015
ATTTTGT150.002195524870.015
CTTTACA1700.067.941181
TTTACAC1750.066.02
CTATACA750.065.3333361
TTACACA1900.060.789473
AAATAAC252.3389646E-456.00000468
ACACCAA252.3389646E-456.00000470
TTTATAC252.3389646E-456.0000043
CTTTATA200.006860934752.52
AGCTCGT200.006860934752.536
GCTCGTA200.006860934752.537
ACATAAT200.006860934752.526
TATTCTG200.006860934752.547
CGAGCAC200.006860934752.512
AATAACA200.006860934752.569
Produced by FastQC (version 0.11.5)