##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780394_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21725 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22909090909091 34.0 34.0 34.0 27.0 34.0 2 32.95116225546605 34.0 34.0 34.0 31.0 34.0 3 33.25348676639816 34.0 34.0 34.0 33.0 34.0 4 33.36883774453395 34.0 34.0 34.0 33.0 34.0 5 33.419378596087455 34.0 34.0 34.0 34.0 34.0 6 37.04405063291139 38.0 37.0 38.0 36.0 38.0 7 36.699194476409666 38.0 37.0 38.0 34.0 38.0 8 36.58421173762946 38.0 37.0 38.0 34.0 38.0 9 36.371093210586885 38.0 37.0 38.0 34.0 38.0 10-11 35.972888377445344 38.0 36.5 38.0 32.5 38.0 12-13 35.79742232451093 38.0 36.0 38.0 31.5 38.0 14-15 35.82052934407365 38.0 36.0 38.0 31.0 38.0 16-17 35.982278481012656 38.0 36.5 38.0 32.5 38.0 18-19 36.10902186421174 38.0 36.5 38.0 33.0 38.0 20-21 35.84674338319908 38.0 36.0 38.0 31.0 38.0 22-23 35.63889528193326 38.0 35.5 38.0 31.5 38.0 24-25 34.617537399309555 37.0 34.0 38.0 27.0 38.0 26-27 35.01739930955121 37.0 35.0 38.0 29.0 38.0 28-29 34.753049482163405 37.0 34.5 38.0 27.0 38.0 30-31 34.2273417721519 37.0 34.0 38.0 25.0 38.0 32-33 34.80752589182969 37.0 35.0 38.0 27.0 38.0 34-35 34.07449942462601 37.0 34.0 38.0 25.0 38.0 36-37 33.96161104718067 37.0 34.0 38.0 24.5 38.0 38-39 33.53189873417722 37.0 34.0 38.0 22.5 38.0 40-41 33.769919447640966 37.0 34.0 38.0 24.5 38.0 42-43 34.65921749136939 37.0 34.5 38.0 26.5 38.0 44-45 34.98761795166858 37.0 35.5 38.0 29.0 38.0 46-47 35.003153049482165 37.0 35.0 38.0 27.0 38.0 48-49 34.52384349827388 37.0 34.5 38.0 25.5 38.0 50-51 34.72536248561565 37.0 34.5 38.0 27.0 38.0 52-53 34.470149597238205 37.0 34.0 38.0 26.5 38.0 54-55 34.783406214039125 37.0 34.5 38.0 27.0 38.0 56-57 34.635765247410816 37.0 34.5 38.0 26.5 38.0 58-59 34.72278481012658 37.0 34.5 38.0 27.0 38.0 60-61 34.46055235903337 37.0 34.5 38.0 25.5 38.0 62-63 35.08959723820483 37.0 35.5 38.0 29.0 38.0 64-65 35.051254315304945 37.0 35.5 38.0 29.0 38.0 66-67 33.48678941311853 37.0 33.5 38.0 22.5 38.0 68-69 30.35560414269275 35.0 24.0 38.0 11.0 38.0 70-71 28.077882623705406 32.5 21.5 37.0 11.0 38.0 72-73 26.659240506329112 29.5 15.0 37.0 11.0 38.0 74-75 26.758826237054087 31.0 11.0 37.0 11.0 38.0 76 26.73463751438435 31.0 11.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29298755860753545 1101 2 0.8663545914749804 1101 3 -0.03877617790656984 1101 4 0.5886882572569689 1101 5 0.32661219693319055 1101 6 -2.2482501845805913 1101 7 0.43631494640617063 1101 8 0.7065547041573979 1101 9 0.1514481295839758 1101 10-11 0.4831056700961369 1101 12-13 1.091788559421559 1101 14-15 1.379922531992129 1101 16-17 -0.0249825070591001 1101 18-19 0.0434294483253268 1101 20-21 -0.06113889841529385 1101 22-23 1.266491985042613 1101 24-25 -1.697352404941313 1101 26-27 0.2794329692824107 1101 28-29 1.5778430173739864 1101 30-31 2.1362315195228945 1101 32-33 0.7319833862799427 1101 34-35 1.691113212021662 1101 36-37 1.4995373248728256 1101 38-39 -0.29676999457416287 1101 40-41 1.5763683638675943 1101 42-43 0.9947428732050341 1101 44-45 1.486561952917988 1101 46-47 1.6424100346164536 1101 48-49 -1.6983906581659483 1101 50-51 -2.4564485554291053 1101 52-53 -2.1688227722079603 1101 54-55 -1.1923631122780662 1101 56-57 -1.5566447715028744 1101 58-59 -1.562473224307496 1101 60-61 -2.0027345904816656 1101 62-63 -1.9512527152616386 1101 64-65 -1.535062368523839 1101 66-67 -1.737952451943194 1101 68-69 -0.4318800306433488 1101 70-71 -0.2868045834433808 1101 72-73 -1.8758131540943879 1101 74-75 0.5465978766732462 1101 76 -0.5209708975284109 1102 1 0.22717316466814452 1102 2 0.8081159551113473 1102 3 0.6667162463358522 1102 4 0.2127412875600001 1102 5 0.33134704541804183 1102 6 0.602602088146682 1102 7 -0.29758656874534495 1102 8 0.8433918253695225 1102 9 1.3072246447354914 1102 10-11 1.253465518580981 1102 12-13 1.0877639382094344 1102 14-15 -0.03248277103816832 1102 16-17 0.03207241718332199 1102 18-19 -0.918455021371642 1102 20-21 1.1422228440089484 1102 22-23 0.8919654698910975 1102 24-25 -4.764350511001922 1102 26-27 -0.6642223337478939 1102 28-29 0.4310627143436818 1102 30-31 2.775436064977441 1102 32-33 1.3079777044617629 1102 34-35 1.6908764695974199 1102 36-37 -1.18535850846051 1102 38-39 1.8189970508803839 1102 40-41 1.732381621443352 1102 42-43 1.4601784792656431 1102 44-45 1.8684274832210193 1102 46-47 1.427447913404329 1102 48-49 -3.582150127862917 1102 50-51 0.182282505176957 1102 52-53 0.5397473035496176 1102 54-55 2.0102884028734493 1102 56-57 1.2788192436486412 1102 58-59 0.4226120029652307 1102 60-61 -5.72763989351197 1102 62-63 -2.0390841546555762 1102 64-65 1.1543315708700987 1102 66-67 2.140125199571955 1102 68-69 -0.8447588185221377 1102 70-71 0.9193980680717715 1102 72-73 -1.920557472276208 1102 74-75 -0.027502502114632676 1102 76 1.857816981259468 1103 1 1.7138629794829612 1103 2 1.1312255230125814 1103 3 0.5768242710272133 1103 4 0.6066456085476517 1103 5 0.4281834651711307 1103 6 0.6911437548133463 1103 7 1.2730152831065098 1103 8 1.105544603147301 1103 9 0.49376013856264933 1103 10-11 0.4396152099390065 1103 12-13 -0.23621368524735686 1103 14-15 0.2898551919247936 1103 16-17 0.8159265838499934 1103 18-19 0.7095349477641619 1103 20-21 0.04856929462622617 1103 22-23 1.143315778533072 1103 24-25 -2.343536467792049 1103 26-27 0.007266863782966482 1103 28-29 0.42604728224491595 1103 30-31 2.1353897686811436 1103 32-33 2.3279429822395414 1103 34-35 1.522763043671496 1103 36-37 -0.3523145269790291 1103 38-39 0.6962154150779156 1103 40-41 0.3401169609495227 1103 42-43 1.0575936027224344 1103 44-45 2.48291436593707 1103 46-47 2.3174942097006195 1103 48-49 -2.4242604673690913 1103 50-51 -0.6674474330946509 1103 52-53 1.5087866554014653 1103 54-55 0.8227884028734493 1103 56-57 1.0603585955004888 1103 58-59 0.390108144940541 1103 60-61 -4.3952710663514765 1103 62-63 -0.5048441855197794 1103 64-65 0.7345784844503456 1103 66-67 0.08331578599170797 1103 68-69 -0.17346252222584013 1103 70-71 0.6795253828865881 1103 72-73 -1.7402912685725056 1103 74-75 -1.3939183971763605 1103 76 -0.8890965989874466 1104 1 0.7471494304909285 1104 2 0.8375858918202113 1104 3 0.771542195702942 1104 4 -0.621515041553927 1104 5 -0.51477637230348 1104 6 0.3915656957416189 1104 7 0.00344191226731283 1104 8 0.5176402430910443 1104 9 1.0590047080266274 1104 10-11 -0.5956246712924411 1104 12-13 0.32184226099424507 1104 14-15 1.1306896973162637 1104 16-17 -0.23048691825971446 1104 18-19 0.5230995672359526 1104 20-21 -0.2522589597885201 1104 22-23 1.0519733812835028 1104 24-25 0.446586988998078 1104 26-27 1.2928583624546448 1104 28-29 2.1007620814322934 1104 30-31 2.058268343458451 1104 32-33 1.7147221664870784 1104 34-35 0.5309674506100777 1104 36-37 2.1788622193875966 1104 38-39 0.979697209108231 1104 40-41 2.3909061467598036 1104 42-43 0.4552338590124734 1104 44-45 0.9314219452463348 1104 46-47 0.9895523437840765 1104 48-49 0.48064654935227225 1104 50-51 1.192765100113661 1104 52-53 1.0619980946888603 1104 54-55 0.8038010611012965 1104 56-57 0.7781269968131994 1104 58-59 -1.8446941679208422 1104 60-61 1.0961338406652459 1104 62-63 0.18213815547100864 1104 64-65 0.9328125835283316 1104 66-67 -0.6212474270103243 1104 68-69 2.7264437131234303 1104 70-71 2.6495206946540506 1104 72-73 1.3534733821541707 1104 74-75 0.7374302510499255 1104 76 0.6742726774619996 1105 1 1.4844140183266816 1105 2 0.6934055892576865 1105 3 0.6049784414578028 1105 4 0.1555766534136609 1105 5 0.23607265517414078 1105 6 0.5610624540003428 1105 7 0.8967731873522169 1105 8 -0.7941843941426754 1105 9 0.5934289130281769 1105 10-11 0.7401271039468327 1105 12-13 -0.7733260008149543 1105 14-15 0.7842093021325596 1105 16-17 -0.015374229158148012 1105 18-19 0.573732478628358 1105 20-21 0.23006583181382467 1105 22-23 0.6537794942813449 1105 24-25 0.0197577207053925 1105 26-27 0.20201242234966799 1105 28-29 1.9211541777583108 1105 30-31 1.2365641137579289 1105 32-33 2.1050432532422505 1105 34-35 -0.40496956698794406 1105 36-37 0.9466917964175394 1105 38-39 0.44380650209989625 1105 40-41 1.2119929019311542 1105 42-43 0.6739742109729576 1105 44-45 1.7912552271234574 1105 46-47 1.4724174255994527 1105 48-49 0.13069285994195923 1105 50-51 1.1407428710306107 1105 52-53 1.8703494376959569 1105 54-55 1.6947396223856472 1105 56-57 0.5743604021852278 1105 58-59 0.9330921858920576 1105 60-61 0.9076497406343762 1105 62-63 0.11545090632003507 1105 64-65 1.6543315708700987 1105 66-67 1.2794163581085414 1105 68-69 1.6278021570876184 1105 70-71 1.5049544705107962 1105 72-73 1.3730781984554987 1105 74-75 0.6312246320317065 1105 76 1.0163535666253232 1106 1 -0.7185988941553845 1106 2 -0.5200090448886527 1106 3 0.13546624633585225 1106 4 0.01605011108941312 1106 5 0.2633323395356868 1106 6 0.627418264617269 1106 7 -0.9906012746277 1106 8 -0.2898802334540065 1106 9 -0.36281211997039264 1106 10-11 0.508061106816271 1106 12-13 1.3970470264447243 1106 14-15 -0.8936960063322843 1106 16-17 0.149259917183322 1106 18-19 0.6399089492165899 1106 20-21 0.5783441675383543 1106 22-23 1.2292816463616916 1106 24-25 0.005410518409846077 1106 26-27 1.123461489781512 1106 28-29 0.9145185966966238 1106 30-31 0.7258037120362673 1106 32-33 -0.030717148479411094 1106 34-35 0.9633948519503619 1106 36-37 2.423556932715961 1106 38-39 0.23397866852744187 1106 40-41 1.179532356737468 1106 42-43 0.2625681851479982 1106 44-45 0.9139238067504323 1106 46-47 1.080021442816097 1106 48-49 1.956912372137083 1106 50-51 0.05268691694166705 1106 52-53 0.7819348035496176 1106 54-55 -0.5816233618324347 1106 56-57 1.1717420377662862 1106 58-59 0.9000936206122887 1106 60-61 1.1648233417821459 1106 62-63 0.8152356982855977 1106 64-65 -0.7427272526593143 1106 66-67 0.2885626995719548 1106 68-69 1.8537705932425688 1106 70-71 -1.0102894319282285 1106 72-73 -1.1131126193350305 1106 74-75 -1.6704252962322776 1106 76 -0.7157124305052385 1107 1 1.4964507620707437 1107 2 -0.05572333060293744 1107 3 0.27507663594624177 1107 4 0.48912115768987263 1107 5 0.40236977269076846 1107 6 0.41408747775707155 1107 7 1.0445400546312769 1107 8 -0.4254799278772339 1107 9 -0.5671665890307409 1107 10-11 0.4776944146848763 1107 12-13 -0.026176808543809216 1107 14-15 0.8723467744163784 1107 16-17 1.129779397702798 1107 18-19 1.1646415695374515 1107 20-21 1.197519110242716 1107 22-23 0.37147034002097 1107 24-25 0.5374960799071715 1107 26-27 -0.23788304803360916 1107 28-29 1.2780594675904382 1107 30-31 0.09402372731510411 1107 32-33 0.8163989706955306 1107 34-35 -0.10325908235063253 1107 36-37 2.1597104850459843 1107 38-39 0.8493339015297323 1107 40-41 1.5677103552095843 1107 42-43 1.503400881863051 1107 44-45 1.1824493988054385 1107 46-47 1.5276914198978346 1107 48-49 1.5169773072020192 1107 50-51 1.9807808818003352 1107 52-53 1.9426490892639023 1107 54-55 0.8877234678085131 1107 56-57 2.734480769622664 1107 58-59 1.1940202821860098 1107 60-61 1.6260532883062098 1107 62-63 0.4762364622275399 1107 64-65 0.8166692332077616 1107 66-67 0.3973289333381871 1107 68-69 1.913358064594746 1107 70-71 0.8906845940457977 1107 72-73 -0.09983912812036522 1107 74-75 -0.5854367553613891 1107 76 -0.20062457718209004 1108 1 -1.167618501998522 1108 2 -0.02334237822198304 1108 3 -0.3278670869974789 1108 4 0.21899128756000152 1108 5 -0.284902954581959 1108 6 -1.082189578519987 1108 7 0.19449676458798848 1108 8 -0.28556650796381433 1108 9 0.31993297806882737 1108 10-11 0.5282571852476465 1108 12-13 1.2339097715427698 1108 14-15 0.08689222896183679 1108 16-17 -0.7774067494833403 1108 18-19 0.2803991452950214 1108 20-21 0.8112853440089438 1108 22-23 0.2680071365577632 1108 24-25 1.0465869889980794 1108 26-27 -0.496930667081223 1108 28-29 -0.328520618989657 1108 30-31 0.8116860649774438 1108 32-33 1.2034985377950989 1108 34-35 0.8220223029307547 1108 36-37 1.16620399153949 1108 38-39 0.5186845508803799 1108 40-41 0.6672774547766807 1108 42-43 -0.4191965207343529 1108 44-45 0.9801983165543504 1108 46-47 1.0478645800709927 1108 48-49 1.9552457054704178 1108 50-51 0.8974908385102935 1108 52-53 0.06360147021628393 1108 54-55 0.1161217362067859 1108 56-57 -0.34482658968468627 1108 58-59 -1.0852004970347693 1108 60-61 0.6709017731546965 1108 62-63 0.9260200120110866 1108 64-65 -0.07233509579656783 1108 66-67 0.13022936623862336 1108 68-69 1.6369078481445314 1108 70-71 -0.8686227652615628 1108 72-73 -1.8639949722762097 1108 74-75 -2.0721900021146347 1108 76 -0.45551635207386454 1109 1 -0.5502271976506954 1109 2 0.6212953029374333 1109 3 0.2477850869155631 1109 4 0.3871072295889846 1109 5 0.2837926975919558 1109 6 0.2128828852481348 1109 7 -0.02811193106418841 1109 8 0.5741436369637256 1109 9 1.4202228331412883 1109 10-11 0.9728948664070671 1109 12-13 0.49593875705001267 1109 14-15 1.2544284608458938 1109 16-17 1.649259917183322 1109 18-19 1.2766310293529983 1109 20-21 1.5472273729944561 1109 22-23 1.4940940930795037 1109 24-25 0.482818873056047 1109 26-27 0.3242287532086223 1109 28-29 -0.0476510537722632 1109 30-31 1.938932441789035 1109 32-33 1.796542016055966 1109 34-35 0.5646309985829276 1109 36-37 1.5285228321192008 1109 38-39 1.0349164349383528 1109 40-41 0.4064078895592971 1109 42-43 1.5428324647728928 1109 44-45 1.7958504904673944 1109 46-47 1.2965602322449072 1109 48-49 1.5166949808327388 1109 50-51 2.111403881988551 1109 52-53 0.1931666876075866 1109 54-55 0.03293333040968349 1109 56-57 0.8461879030689303 1109 58-59 -0.1576642651507072 1109 60-61 2.0674235122851314 1109 62-63 1.1129765337502207 1109 64-65 1.9441866433338646 1109 66-67 2.2360264676878927 1109 68-69 -0.962512441710544 1109 70-71 -0.17152131598619746 1109 72-73 0.17919343352089356 1109 74-75 -0.766972610810285 1109 76 -0.5552264970014029 1110 1 -0.6091485566433299 1110 2 -0.14705822521651868 1110 3 0.08218755781125964 1110 4 -0.1842874009645925 1110 5 0.39640852082787603 1110 6 0.2539852848679942 1110 7 0.55231097223826 1110 8 0.5736684647137835 1110 9 1.076435710309262 1110 10-11 -1.156114399451809 1110 12-13 1.015658405422549 1110 14-15 1.2531217371585583 1110 16-17 0.8049976221013537 1110 18-19 0.15569969174310927 1110 20-21 1.4394820653204263 1110 22-23 0.23904538792388763 1110 24-25 -0.9058720273953611 1110 26-27 1.9108288957602966 1110 28-29 1.5166159930322038 1110 30-31 0.1857844256331802 1110 32-33 1.1157389749535724 1110 34-35 1.4568856909088979 1110 36-37 2.0678433358017827 1110 38-39 2.1462255344869448 1110 40-41 -0.30540014085173084 1110 42-43 0.8057215120525285 1110 44-45 1.1193239996144584 1110 46-47 1.3244766019289145 1110 48-49 2.6474861426288854 1110 50-51 -0.6335474128558332 1110 52-53 1.099557754369286 1110 54-55 0.37196873074230297 1110 56-57 1.847851005943724 1110 58-59 0.013160158702937963 1110 60-61 0.47702199173392756 1110 62-63 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-2.480803564265031 2116 66-67 -0.5661670301577715 2116 68-69 -2.875164223927545 2116 70-71 -1.108262404901204 2116 72-73 -0.48453551281674834 2116 74-75 -0.8457035156281449 2116 76 -4.480020856578371 2117 1 0.02026028588026918 2117 2 0.07847580359619855 2117 3 0.0066783675479769045 2117 4 0.49777916634787545 2117 5 0.038733409054408696 2117 6 -0.8240077603381692 2117 7 0.2999513100425304 2117 8 0.630797128399827 2117 9 -0.818248840112993 2117 10-11 -0.2517428147523475 2117 12-13 -2.3551811375481435 2117 14-15 -0.8397744377048397 2117 16-17 -1.5780128100894046 2117 18-19 -2.0929341880383134 2117 20-21 -0.6747752620516607 2117 22-23 0.13770410625473062 2117 24-25 -1.2200796776685863 2117 26-27 -2.872082182232738 2117 28-29 -2.4297327402017714 2117 30-31 -1.9016472683558945 2117 32-33 -4.389228734932175 2117 34-35 0.9335374544459043 2117 36-37 -1.8640990387635412 2117 38-39 1.241108793304626 2117 40-41 -1.7721164846172535 2117 42-43 1.3432277216898854 2117 44-45 -1.0210138046577697 2117 46-47 -2.3575899653835464 2117 48-49 -2.9332391430144327 2117 50-51 -1.2746303736109184 2117 52-53 0.535722682337493 2117 54-55 -1.0408479607629104 2117 56-57 0.2691128042547035 2117 58-59 -1.2821701940044647 2117 60-61 -1.5709164086634857 2117 62-63 -1.3679193819283029 2117 64-65 -2.81536539882687 2117 66-67 -1.0334069973977407 2117 68-69 0.4999381511748311 2117 70-71 -0.2565015531403496 2117 72-73 0.38933836105712416 2117 74-75 1.682961513036883 2117 76 0.9941806176231047 2118 1 -1.306834188273033 2118 2 0.7594027198172313 2118 3 -0.49688669484061876 2118 4 0.44252069932470306 2118 5 -0.06019707222901616 2118 6 0.642124146970211 2118 7 0.8035163724311261 2118 8 0.23953153125187754 2118 9 -2.0098709434998057 2118 10-11 -0.10958595200725085 2118 12-13 -1.1617765029670366 2118 14-15 0.09159811131477369 2118 16-17 -2.909563612228446 2118 18-19 -0.17626752137164203 2118 20-21 -0.4951852442263416 2118 22-23 0.42781105812639453 2118 24-25 1.358351694880433 2118 26-27 -4.053009098453778 2118 28-29 -2.7987166974210247 2118 30-31 -2.730078640904914 2118 32-33 -1.3468936190676501 2118 34-35 -1.1395463245202322 2118 36-37 -4.819090126107568 2118 38-39 -1.2660213314725581 2118 40-41 -1.048408819733119 2118 42-43 -0.25213769720494383 2118 44-45 -2.9831350167789807 2118 46-47 -2.317037380713323 2118 48-49 0.023088842725314862 2118 50-51 -1.484077788940688 2118 52-53 -3.6224769611562664 2118 54-55 -2.971329244185373 2118 56-57 -2.467963844586656 2118 58-59 -2.1146122617406533 2118 60-61 -1.6587060699825642 2118 62-63 -1.8465290075967502 2118 64-65 0.2131551002818668 2118 66-67 -0.05702553572216118 2118 68-69 -3.2050529361691957 2118 70-71 -1.2676423731046995 2118 72-73 -1.6351714428644435 2118 74-75 -0.9939547079969877 2118 76 -0.31865360697582545 2119 1 0.8990481646681445 2119 2 -1.7329720078516146 2119 3 -0.44786708699748345 2119 4 -1.010638342069626 2119 5 -2.1026807323597367 2119 6 -0.8273747637051727 2119 7 -0.9121699020786806 2119 8 -0.3388998412971418 2119 9 -3.037104058968211 2119 10-11 -1.1961872591967975 2119 12-13 -0.16831245067945133 2119 14-15 -0.3212559191863207 2119 16-17 -0.3877771198537161 2119 18-19 -2.0558971510012753 2119 20-21 -2.698344285620678 2119 22-23 -1.3319928634422311 2119 24-25 0.6688092112202995 2119 26-27 0.5504767403261823 2119 28-29 -0.7900021004711277 2119 30-31 -0.14238800909663496 2119 32-33 -0.17205701776046567 2119 34-35 -0.8476073266988777 2119 36-37 -0.9078700825345862 2119 38-39 -3.42724137504554 2119 40-41 -0.7401299526307241 2119 42-43 0.19339607185823837 2119 44-45 -0.49239427603824026 2119 46-47 -0.015839123632709118 2119 48-49 -0.4180876278629171 2119 50-51 1.6782315792510332 2119 52-53 1.0458236924385034 2119 54-55 1.3227884028734493 2119 56-57 -2.0137154785735802 2119 58-59 -0.2703856822199526 2119 60-61 0.5368276990806251 2119 62-63 -0.7517577657666905 2119 64-65 -0.734557318018787 2119 66-67 0.9968960329052905 2119 68-69 -0.46975881852213774 2119 70-71 -1.4871412837800833 2119 72-73 -1.2773283056095401 2119 74-75 -0.40626407618870886 2119 76 -0.9014422779997915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 5.0 23 15.0 24 36.0 25 98.0 26 166.0 27 286.0 28 459.0 29 761.0 30 1068.0 31 1495.0 32 2087.0 33 2957.0 34 3611.0 35 3363.0 36 2803.0 37 2511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.668584579976987 32.552359033371694 10.039125431530495 37.739930955120826 2 9.353279631760644 12.846950517836595 63.04257767548906 14.757192174913694 3 8.602991944764096 36.41426927502877 41.66168009205984 13.321058688147296 4 7.102416570771002 25.003452243958574 34.029919447640964 33.86421173762946 5 6.398158803222094 53.66168009205984 30.490218642117377 9.44994246260069 6 12.299194476409667 34.692750287687 12.925201380897583 40.08285385500575 7 12.28078250863061 52.1150747986191 13.77675489067894 21.827387802071346 8 10.794016110471807 31.24510932105869 31.171461449942463 26.789413118527044 9 11.153049482163405 24.368239355581128 13.532796317606444 50.94591484464902 10-11 12.170310701956272 19.98158803222094 36.080552359033376 31.76754890678941 12-13 21.63866513233602 12.915995397008057 27.65247410817031 37.792865362485614 14-15 27.208285385500574 23.04718066743383 15.951668584579979 33.792865362485614 16-17 25.102416570771002 25.14614499424626 13.576524741081702 36.17491369390103 18-19 18.856156501726122 26.858457997698505 13.12082853855006 41.164556962025316 20-21 21.87802071346375 21.355581127733025 13.258918296892979 43.50747986191024 22-23 28.63060989643268 31.912543153049484 13.109321058688147 26.347525891829687 24-25 24.40506329113924 37.36018411967779 13.940161104718065 24.294591484464902 26-27 18.840046029919446 27.693901035673186 34.75028768699655 18.71576524741082 28-29 23.523590333716914 26.464902186421174 32.059838895281935 17.951668584579977 30-31 21.9102416570771 21.166858457997698 23.518987341772153 33.40391254315305 32-33 13.438434982738782 21.24741081703107 25.171461449942463 40.14269275028769 34-35 10.817031070195627 26.69965477560414 30.411967779056386 32.07134637514385 36-37 11.275028768699654 25.212888377445342 36.989643268124276 26.522439585730723 38-39 20.771001150747985 17.58573072497123 28.34752589182969 33.29574223245109 40-41 17.95857307249712 23.94476409666283 32.92520138089758 25.171461449942463 42-43 27.58573072497123 20.73187571921749 37.373993095512084 14.308400460299195 44-45 19.042577675489067 20.23705408515535 27.986191024165706 32.734177215189874 46-47 28.773302646720367 14.411967779056386 27.005753739930956 29.80897583429229 48-49 22.78481012658228 13.620253164556962 31.523590333716918 32.07134637514385 50-51 12.985040276179516 14.006904487917145 39.06098964326812 33.94706559263521 52-53 20.579976985040275 13.546605293440736 44.487917146144994 21.385500575373992 54-55 17.307249712313002 13.581127733026468 39.58342922899885 29.52819332566168 56-57 27.256616800920597 14.009205983889528 34.68584579976985 24.048331415420023 58-59 18.331415420023013 35.13924050632911 32.14499424626007 14.384349827387801 60-61 16.95281933256617 53.072497123130034 17.937859608745686 12.036823935558113 62-63 11.005753739930956 63.00345224395857 13.733026467203683 12.25776754890679 64-65 10.006904487917147 64.34982738780207 13.509781357882625 12.133486766398159 66-67 9.505178365937859 62.71346375143844 13.53739930955121 14.243958573072495 68-69 10.209436133486767 56.478711162255465 14.462600690448793 18.849252013808975 70-71 11.196777905638665 50.533947065592635 16.395857307249713 21.873417721518987 72-73 11.120828538550057 44.33141542002301 18.117376294591484 26.430379746835442 74-75 12.02301495972382 43.12313003452244 17.868814729574222 26.985040276179518 76 11.189873417721518 44.38204833141542 17.219792865362486 27.208285385500574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.5 15 1.5 16 1.0 17 0.0 18 1.0 19 3.5 20 6.0 21 7.0 22 5.0 23 12.0 24 21.5 25 22.0 26 25.0 27 40.5 28 56.5 29 60.0 30 73.0 31 112.5 32 160.5 33 182.0 34 227.0 35 283.0 36 317.5 37 341.0 38 434.5 39 773.5 40 1635.5 41 2805.5 42 3359.0 43 3345.5 44 2994.5 45 2264.0 46 1871.0 47 1591.0 48 1150.5 49 884.5 50 779.0 51 651.5 52 455.0 53 366.5 54 347.0 55 305.5 56 222.5 57 158.0 58 135.0 59 110.5 60 71.5 61 51.0 62 45.0 63 35.0 64 25.5 65 19.5 66 10.0 67 7.0 68 7.0 69 4.0 70 1.0 71 1.0 72 1.5 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 21725.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.45569620253165 #Duplication Level Percentage of deduplicated Percentage of total 1 77.44874715261959 34.43037974683544 2 13.346448540070407 11.866513233601841 3 4.483329881963139 5.979286536248562 4 2.019051563470698 3.5903337169159957 5 0.9008076206253882 2.002301495972382 6 0.4245185338579416 1.1323360184119677 7 0.2795609857113274 0.8699654775604143 8 0.18637399047421827 0.6628308400460299 9 0.08283288465520812 0.33141542002301494 >10 0.641954856077863 5.468354430379747 >50 0.09318699523710913 2.959723820483314 >100 0.062124663491406086 5.390103567318757 >500 0.0 0.0 >1k 0.031062331745703043 25.31645569620253 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT 3066 14.112773302646719 RNA PCR Primer, Index 27 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 1291 5.942462600690448 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 1143 5.261219792865362 No Hit CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 442 2.034522439585731 No Hit TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 241 1.1093210586881472 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 133 0.6121979286536249 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 122 0.5615650172612198 No Hit CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 118 0.5431530494821634 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 115 0.5293440736478712 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 99 0.4556962025316456 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT 92 0.42347525891829685 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 88 0.40506329113924056 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT 64 0.2945914844649022 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT 64 0.2945914844649022 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT 63 0.2899884925201381 No Hit CTTATCACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 62 0.28538550057537404 No Hit CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 60 0.2761795166858458 No Hit ATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGTCTT 51 0.2347525891829689 TruSeq Adapter, Index 4 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT 45 0.20713463751438435 RNA PCR Primer, Index 27 (95% over 23bp) CTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 40 0.18411967779056385 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT 40 0.18411967779056385 No Hit TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 39 0.17951668584579977 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT 37 0.17031070195627157 No Hit ATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGTCTT 37 0.17031070195627157 Illumina PCR Primer Index 6 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGTCTT 36 0.16570771001150747 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 36 0.16570771001150747 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGTCTT 35 0.1611047180667434 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGT 35 0.1611047180667434 No Hit CTTATACACATCTCCGAGCCCACGCGACTTGACCCTATCTCGTATGCCGT 32 0.1472957422324511 No Hit ATACACATCTCCGAGCCCACGAGAATTGACCCTATCTCGTATGCCGTCTT 31 0.14269275028768702 No Hit CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 30 0.1380897583429229 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCCT 27 0.12428078250863062 No Hit TCTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 27 0.12428078250863062 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTC 27 0.12428078250863062 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 26 0.11967779056386652 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTC 25 0.11507479861910241 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTC 24 0.11047180667433831 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCAGTCTT 24 0.11047180667433831 No Hit ATACACATCTCCGAGCCCACGAGCCTTGACCCTATCTCGTATGCCGTCTT 23 0.10586881472957421 RNA PCR Primer, Index 27 (95% over 21bp) TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 22 0.10126582278481014 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.004602991944764097 0.0 13 0.0 0.0 0.0 0.004602991944764097 0.0 14 0.0 0.0 0.0 0.004602991944764097 0.0 15 0.0 0.0 0.0 0.004602991944764097 0.0 16 0.0 0.0 0.0 0.004602991944764097 0.0 17 0.0 0.0 0.0 0.004602991944764097 0.0 18 0.0 0.0 0.0 0.009205983889528193 0.0 19 0.0 0.0 0.0 0.009205983889528193 0.0 20 0.0 0.0 0.0 0.009205983889528193 0.0 21 0.0 0.0 0.0 0.009205983889528193 0.0 22 0.0 0.0 0.0 0.009205983889528193 0.0 23 0.0 0.0 0.0 0.01380897583429229 0.0 24 0.0 0.0 0.0 0.018411967779056387 0.0 25 0.0 0.0 0.0 0.018411967779056387 0.0 26 0.0 0.0 0.0 0.023014959723820484 0.0 27 0.0 0.0 0.0 0.02761795166858458 0.0 28 0.0 0.0 0.0 0.03682393555811277 0.0 29 0.0 0.0 0.0 0.04142692750287687 0.0 30 0.0 0.0 0.0 0.04602991944764097 0.0 31 0.0 0.0 0.0 0.04602991944764097 0.0 32 0.0 0.0 0.0 0.05523590333716916 0.0 33 0.0 0.0 0.0 0.06444188722669736 0.0 34 0.0 0.0 0.0 0.10126582278481013 0.0 35 0.0 0.0 0.0 0.11047180667433831 0.0 36 0.0 0.0 0.0 0.1611047180667434 0.0 37 0.0 0.0 0.0 0.23014959723820483 0.0 38 0.0 0.0 0.0 0.3176064441887227 0.0 39 0.0 0.0 0.0 0.4142692750287687 0.0 40 0.0 0.0 0.0 0.5477560414269275 0.0 41 0.0 0.0 0.0 0.6812428078250863 0.0 42 0.0 0.0 0.0 0.8193325661680092 0.0 43 0.0 0.0 0.0 0.9574223245109321 0.0 44 0.0 0.0 0.0 1.1369390103567318 0.0 45 0.0 0.0 0.0 1.3164556962025316 0.0 46 0.0 0.0 0.0 1.4499424626006905 0.0 47 0.0 0.0 0.0 1.6432681242807825 0.0 48 0.0 0.0 0.0 1.7951668584579976 0.0 49 0.0 0.0 0.0 1.9332566168009206 0.0 50 0.0 0.0 0.0 2.1173762945914847 0.0 51 0.0 0.0 0.0 2.3659378596087457 0.0 52 0.0 0.0 0.0 2.4948216340621405 0.0 53 0.0 0.0 0.0 2.655926352128884 0.0 54 0.0 0.0 0.0 2.8262370540851554 0.0 55 0.0 0.0 0.0 3.028768699654776 0.0 56 0.0 0.0 0.0 3.2220943613348676 0.0 57 0.0 0.0 0.0 3.3739930955120827 0.0 58 0.0 0.0 0.0 3.5120828538550057 0.0 59 0.0 0.0 0.0 3.663981588032221 0.0 60 0.0 0.0 0.0 3.8757192174913695 0.0 61 0.0 0.0 0.0 4.018411967779056 0.0 62 0.0 0.0 0.0 4.151898734177215 0.0 63 0.0 0.0 0.0 4.3544303797468356 0.0 64 0.0 0.0 0.0 4.487917146144994 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGAG 15 0.0021955248 70.0 17 AACCTCC 15 0.0021955248 70.0 40 TAACCTC 15 0.0021955248 70.0 39 TCTTTAT 15 0.0021955248 70.0 1 GCACACG 15 0.0021955248 70.0 15 ATTTTGT 15 0.0021955248 70.0 15 CTTTACA 170 0.0 67.94118 1 TTTACAC 175 0.0 66.0 2 CTATACA 75 0.0 65.333336 1 TTACACA 190 0.0 60.78947 3 AAATAAC 25 2.3389646E-4 56.000004 68 ACACCAA 25 2.3389646E-4 56.000004 70 TTTATAC 25 2.3389646E-4 56.000004 3 CTTTATA 20 0.0068609347 52.5 2 AGCTCGT 20 0.0068609347 52.5 36 GCTCGTA 20 0.0068609347 52.5 37 ACATAAT 20 0.0068609347 52.5 26 TATTCTG 20 0.0068609347 52.5 47 CGAGCAC 20 0.0068609347 52.5 12 AATAACA 20 0.0068609347 52.5 69 >>END_MODULE