FastQCFastQC Report
Wed 25 May 2016
SRR1780393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214528
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCT250.002350691535.19328326
CTTTACA1800.031.793961
CTTATGC353.19953E-431.444581
TTAATGC353.2170987E-431.41524541
GTTACTC300.00570730529.36198218
CGCTACG300.0057203529.34827416
CTTCTCG300.0057203529.34827438
AACACTG300.0057203529.34827423
ATTCCGC300.005733418329.3345812
GTCTATC300.005739961829.32773827
GATTCCG300.005739961829.32773811
CTAGGTC300.005739961829.32773833
ACAGACG300.00575962629.3072268
TTTACAC2050.028.9498212
CACTAAG508.2812854E-526.4134451
TAAGCCA508.360554E-526.3765034
CGGTGGT1450.025.77615543
CTATACA1051.10958354E-1025.1556631
ATCGTTG450.001392228524.45118740
TGGGACA450.001401724324.422694
Produced by FastQC (version 0.11.5)