FastQCFastQC Report
Wed 25 May 2016
SRR1780393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214528
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT2878713.418761187350835No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT2781912.96753803699284Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC91504.265177505966587No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC48752.272430638424821No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG34191.5937313544152745No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT24451.1397113663484486No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC16900.7877759546539379No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC14240.663782816229117No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG12880.6003878281622912No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9070.42278863365155134No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG9020.4204579355608592No Hit
CTTAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC8650.4032107696897375No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC6190.28854042362768495No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG4880.2274761336515513No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG4780.22281473747016706No Hit
CTTATCACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC4240.1976431980906921No Hit
CTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT3590.1673441229116945No Hit
ACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTTCT3070.14310486276849643Illumina PCR Primer Index 10 (95% over 23bp)
TCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT2940.1370450477326969No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC2240.10441527446300716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT27500.042.63638744
TCTTTAT2450.041.3411671
CTTTACA9450.040.542311
TTTACAC9450.040.2811132
AGCGGAT452.3374014E-839.10779635
GCCGTCT30450.038.50576443
CTATACA6600.038.3658561
TTACACA10350.036.7784043
TGCTAGC301.2982165E-436.66355544
CTTATAA1000.035.2298661
TCGGCTT250.00234678535.20522314
GTTCCAC250.00234678535.20522313
ACGACCG250.00234678535.20522315
ATGATTC505.9115337E-835.19701440
ATTGGCG505.9115337E-835.19701421
TGTGTAC250.002349471735.19701427
CGCCATT505.9115337E-835.19701417
CTTTATA2950.035.056052
CTTATAC36750.034.9902041
TATATAC451.0307431E-634.22733
Produced by FastQC (version 0.11.5)