FastQCFastQC Report
Wed 25 May 2016
SRR1780392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21109
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCG150.002195855469.9905245
TGGTCGC200.006861978752.49289346
TATACCC200.006861978752.4928933
CTAATCT200.006861978752.4928936
CCCCCGA200.006861978752.49289320
TGTGTAG200.006861978752.49289332
GACGAAC404.4461187E-543.74407620
CCCTCCT850.003673508320.5854477
CCGACGA1501.38795385E-518.66413918
TGCCGAC1602.4195624E-517.49763116
GCCGACG1502.9822887E-416.33112117
CTGCCGA1755.2198557E-515.99783315
GCTGCCG1850.001463803213.2414514
CGCTGCC1950.002175793512.56240113
ACGCTGC2100.003792704511.66508712
TATACAC2550.0012650710.9789053
TACACAT2600.001487909810.7677732
CTTATAC2350.00816421110.5238721
CACATCT2700.002038071910.3689664
ATACACA3206.751766E-49.9366031
Produced by FastQC (version 0.11.5)