FastQCFastQC Report
Wed 25 May 2016
SRR1780392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21109
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT318615.09308825619404Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT16437.783409919939363No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC13226.262731536311526No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC4752.25022502250225No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT2871.3596096451750437Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1890.8953526931640532No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC1380.6537495854848643No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT1240.5874271637690085No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1170.5542659529110806No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT1130.5353166895636932No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1070.5068927945426122No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1000.47373158368468427No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG860.4074091619688284No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT840.39793453029513476No Hit
CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT690.32687479274243214Illumina PCR Primer Index 1 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC660.3126628452318916No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT580.27476431853711686No Hit
TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC570.27002700270027No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC560.2652896868634232No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG540.2558150551897295No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT480.22739116016864844No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT430.2037045809844142No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG400.1894926334738737No Hit
ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT350.1658060542896395Illumina PCR Primer Index 1 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC340.16106873845279263No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT320.15159410677909896No Hit
CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT310.14685679094225212No Hit
ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT290.13738215926855843TruSeq Adapter, Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT270.12790752759486476Illumina PCR Primer Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCCT270.12790752759486476No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG250.11843289592117107No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT250.11843289592117107No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT250.11843289592117107No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT250.11843289592117107No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC250.11843289592117107No Hit
ATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGTCTT240.11369558008432422Illumina PCR Primer Index 1 (95% over 22bp)
TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT240.11369558008432422No Hit
ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT230.10895826424747738TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT220.10422094841063052No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC150.002194676570.027
ATTACAC150.002194676570.04
TCGTCAC150.002194676570.029
AAAACCT150.002194676570.067
AGACTCG150.002194676570.025
TTAACAC353.4833647E-970.02
CTCGTCA150.002194676570.028
GAGACTC150.002194676570.024
TCTTAAT207.7499215E-570.01
TTTACAC1650.067.8787842
CTTTACA1800.062.2222181
CTTAACA401.0013537E-861.251
TTACACA1900.058.9473723
TCTTTAT308.099712E-658.3333321
TAACACA452.5374902E-854.4444433
CGTCACA200.006858305552.530
TTGACAA200.006858305552.558
CTTGACA200.006858305552.557
CTTGAAC200.006858305552.557
AAAAATT200.006858305552.563
Produced by FastQC (version 0.11.5)