##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780392_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21109 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48818039698707 34.0 34.0 34.0 31.0 34.0 2 33.06888057226775 34.0 34.0 34.0 32.0 34.0 3 33.305083139892936 34.0 34.0 34.0 33.0 34.0 4 33.40859349092804 34.0 34.0 34.0 33.0 34.0 5 33.42512672319864 34.0 34.0 34.0 34.0 34.0 6 37.14349329669809 38.0 37.0 38.0 36.0 38.0 7 36.77147188403051 38.0 37.0 38.0 34.0 38.0 8 36.746932587995644 38.0 37.0 38.0 34.0 38.0 9 36.499739447628976 38.0 37.0 38.0 34.0 38.0 10-11 36.13780851769387 38.0 36.5 38.0 32.5 38.0 12-13 35.84070775498603 38.0 36.5 38.0 31.5 38.0 14-15 35.90556160879245 38.0 36.0 38.0 31.5 38.0 16-17 35.98846463593728 38.0 36.5 38.0 32.5 38.0 18-19 36.12894973707897 38.0 36.5 38.0 33.0 38.0 20-21 35.91709697285518 38.0 36.0 38.0 31.5 38.0 22-23 35.80008527168506 38.0 36.5 38.0 32.5 38.0 24-25 34.63712160689754 37.0 34.5 38.0 28.0 38.0 26-27 35.019541427826994 37.0 35.0 38.0 29.0 38.0 28-29 34.94296271732436 37.0 35.5 38.0 27.0 38.0 30-31 34.65268368942158 37.0 34.5 38.0 26.5 38.0 32-33 35.29764555402909 37.0 36.0 38.0 31.0 38.0 34-35 34.43931498412999 37.0 35.0 38.0 25.5 38.0 36-37 34.08186081766071 37.0 34.0 38.0 25.0 38.0 38-39 33.324364015348905 37.0 34.0 38.0 22.0 38.0 40-41 33.805462125159885 37.0 34.0 38.0 24.5 38.0 42-43 34.84741105689516 37.0 34.5 38.0 29.0 38.0 44-45 35.20135960964517 37.0 35.5 38.0 29.0 38.0 46-47 35.21391349661282 37.0 36.0 38.0 31.0 38.0 48-49 34.686697617130136 37.0 34.5 38.0 28.0 38.0 50-51 34.88670709176181 37.0 34.5 38.0 27.0 38.0 52-53 34.604055142356344 37.0 34.0 38.0 27.0 38.0 54-55 34.85160358141077 37.0 35.0 38.0 27.0 38.0 56-57 34.79636647875314 37.0 35.0 38.0 27.0 38.0 58-59 34.78722345918803 37.0 35.0 38.0 27.0 38.0 60-61 34.62414136150457 37.0 34.5 38.0 26.0 38.0 62-63 35.24442181060211 37.0 36.0 38.0 31.0 38.0 64-65 35.19730920460467 37.5 36.0 38.0 29.0 38.0 66-67 33.50177649343882 37.0 33.5 38.0 22.5 38.0 68-69 30.175896537022123 35.0 24.0 38.0 11.0 38.0 70-71 27.606376427116395 32.5 20.0 37.0 11.0 38.0 72-73 25.826732673267326 26.0 11.0 37.0 11.0 38.0 74-75 25.821355819792508 27.0 11.0 37.0 11.0 38.0 76 25.886920270974464 27.0 11.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1974573398831012 1101 2 0.8235567279275884 1101 3 0.4803964553587221 1101 4 0.13728503497400624 1101 5 0.24873698349374251 1101 6 -1.3377363846893857 1101 7 0.36922488273373943 1101 8 0.5192210025723156 1101 9 0.33220139689559147 1101 10-11 0.22648767406295178 1101 12-13 1.4917823143352393 1101 14-15 1.2763750349401235 1101 16-17 0.6897490328066311 1101 18-19 0.1274353422930048 1101 20-21 0.24309598758075168 1101 22-23 0.7910964049188323 1101 24-25 -1.7327690859912295 1101 26-27 -1.478535554237908 1101 28-29 1.3613489251080608 1101 30-31 0.8615626313308837 1101 32-33 0.5162252479191807 1101 34-35 0.77217560127594 1101 36-37 0.8818773936267803 1101 38-39 -0.006586333419896562 1101 40-41 1.5208575899640664 1101 42-43 1.0512738187458766 1101 44-45 0.5206717283682707 1101 46-47 1.7246972433551377 1101 48-49 -1.9779335321575005 1101 50-51 -1.6380770532261266 1101 52-53 -2.0083678547274744 1101 54-55 -1.2601627333268155 1101 56-57 -1.8982600558368716 1101 58-59 -2.151361944003 1101 60-61 -1.6476234765923223 1101 62-63 -0.2586477870522117 1101 64-65 -0.8710023303218009 1101 66-67 -2.075951157574899 1101 68-69 -2.153418320964157 1101 70-71 -1.7458897669397224 1101 72-73 -1.9782725147227005 1101 74-75 -0.8163717035764044 1101 76 0.41866202320207435 1102 1 -2.0594871045613417 1102 2 -0.05020319270732898 1102 3 -0.28547656051429726 1102 4 -0.39247686978789886 1102 5 0.2911476977794578 1102 6 -0.11849035294335408 1102 7 0.1321217081305619 1102 8 -0.17522344187212724 1102 9 0.2962390953082874 1102 10-11 0.6142604915232681 1102 12-13 0.4883100921130179 1102 14-15 -0.2437142507741612 1102 16-17 -0.5686835068759137 1102 18-19 -0.7576093005641411 1102 20-21 0.6824561066283721 1102 22-23 0.8028771588870853 1102 24-25 -5.566970474880122 1102 26-27 -0.7967399193172753 1102 28-29 -0.10120067806654731 1102 30-31 2.019672750378504 1102 32-33 0.19405262887156027 1102 34-35 1.1185297679426114 1102 36-37 -2.572239669865283 1102 38-39 -0.5434166905627578 1102 40-41 1.2497762407577113 1102 42-43 0.5739672711268256 1102 44-45 1.0955725220190686 1102 46-47 1.4609323624027581 1102 48-49 -4.235498016284481 1102 50-51 -1.7620849897340634 1102 52-53 -1.2359224182195376 1102 54-55 0.6805614730223937 1102 56-57 1.0446962933694763 1102 58-59 0.19412416710811442 1102 60-61 -5.668332801989145 1102 62-63 -2.765840247369674 1102 64-65 0.37192921729725015 1102 66-67 1.739525032901291 1102 68-69 -1.4037903447736824 1102 70-71 0.39609932036186635 1102 72-73 -3.9429302528179413 1102 74-75 -1.1921157511954519 1102 76 0.17287829304334323 1103 1 0.6612731293567862 1103 2 0.6467475174012733 1103 3 0.05934382377977698 1103 4 0.4596534560266363 1103 5 0.24791461507268764 1103 6 0.36292151004745676 1103 7 1.069882777470582 1103 8 0.7034315288881032 1103 9 0.29519481794822156 1103 10-11 -0.10451561541073318 1103 12-13 -0.9342045277700208 1103 14-15 -0.444842070323034 1103 16-17 0.2407358749118913 1103 18-19 1.0431425791351145 1103 20-21 -0.647117828208728 1103 22-23 0.6344352207083048 1103 24-25 -3.092555270201757 1103 26-27 -1.0369237121326478 1103 28-29 -0.8335523906814117 1103 30-31 1.1572040786993085 1103 32-33 1.431521300550763 1103 34-35 0.5637052065390975 1103 36-37 -1.2126949747942746 1103 38-39 -1.2915369913146293 1103 40-41 -0.7825963574043584 1103 42-43 -1.1810452602014934 1103 44-45 1.0979743599472158 1103 46-47 1.111621585460398 1103 48-49 -2.264528926894343 1103 50-51 -0.7203138953313868 1103 52-53 0.20914859264095043 1103 54-55 -0.49289299648470575 1103 56-57 -0.11372058215265923 1103 58-59 -0.3709343124240547 1103 60-61 -5.171883345013374 1103 62-63 -1.1805227870522117 1103 64-65 0.13352069599399385 1103 66-67 -1.0101616838906935 1103 68-69 -0.1628755578062595 1103 70-71 1.0522187856918563 1103 72-73 -2.7772034357753306 1103 74-75 -0.04581249305008939 1103 76 -1.3601208715347681 1104 1 0.10326719903802939 1104 2 0.39155848849097197 1104 3 0.2515232159221057 1104 4 0.11791883779090995 1104 5 0.11207325109937472 1104 6 0.28638333362047064 1104 7 0.9968657278041633 1104 8 0.8466857913047079 1104 9 0.8372630166139032 1104 10-11 -0.7877068681905683 1104 12-13 0.5626449903915756 1104 14-15 0.06312679550349998 1104 16-17 -0.36702913620744937 1104 18-19 0.6246844620113094 1104 20-21 -0.11969010396854429 1104 22-23 0.5559511584399601 1104 24-25 0.0754395407693309 1104 26-27 1.0894662063254685 1104 28-29 1.1005654744038367 1104 30-31 0.6752730186548277 1104 32-33 0.4821143324262209 1104 34-35 0.7124264815576353 1104 36-37 1.6180129570070605 1104 38-39 1.5531627862984152 1104 40-41 1.1060248434851943 1104 42-43 0.825811670858549 1104 44-45 1.154034404424607 1104 46-47 1.3207579827917613 1104 48-49 0.41060079882841904 1104 50-51 1.604000411562609 1104 52-53 0.9965837297795659 1104 54-55 0.5164657877999446 1104 56-57 0.019433606134960257 1104 58-59 -1.2409306059748246 1104 60-61 0.34016261495697364 1104 62-63 -0.22101574479869157 1104 64-65 0.6200848175655267 1104 66-67 -0.38757087588475514 1104 68-69 1.7960755170640113 1104 70-71 0.7735864654546454 1104 72-73 -1.5146941696522767 1104 74-75 -1.4402713514637284 1104 76 -1.8293573429951095 1105 1 1.1182906732164355 1105 2 0.35043172792758526 1105 3 0.690396455358723 1105 4 0.2322850349740051 1105 5 -0.1031380165062572 1105 6 0.08976361531061627 1105 7 0.94672488273374 1105 8 0.0825543359056482 1105 9 1.2644930635622558 1105 10-11 0.4634668407296161 1105 12-13 -0.16946768566475612 1105 14-15 0.25533336827345465 1105 16-17 -0.062334300526700304 1105 18-19 -0.2880854910403272 1105 20-21 0.36340848758074884 1105 22-23 0.06224223825216768 1105 24-25 0.6552517473421062 1105 26-27 0.7883394457620909 1105 28-29 1.4419739251080586 1105 30-31 1.7272917979975517 1105 32-33 0.8464335812525121 1105 34-35 -0.741470232057388 1105 36-37 0.7512523936267783 1105 38-39 -0.23960716675323113 1105 40-41 0.46564925663073353 1105 42-43 0.39491965207921 1105 44-45 1.535255061701605 1105 46-47 1.5285514100218052 1105 48-49 -1.2980376988241673 1105 50-51 0.6677562801072057 1105 52-53 0.20090297860586048 1105 54-55 0.36087893333985477 1105 56-57 0.12215661082979778 1105 58-59 0.5363463893303333 1105 60-61 1.103730690074343 1105 62-63 -1.4988561203855397 1105 64-65 0.6043101696782003 1105 66-67 1.6023821757584287 1105 68-69 0.5200191790358417 1105 70-71 0.288797733060278 1105 72-73 0.09516498527730022 1105 74-75 -0.2978300369097404 1105 76 1.1563703565354082 1106 1 -1.2817093267835666 1106 2 -0.12956827207241162 1106 3 -0.2029368779746079 1106 4 0.34561836830734194 1106 5 -0.08980468317292178 1106 6 -0.63356971802272 1106 7 -1.476608450599592 1106 8 0.10255433590565133 1106 9 -0.8688402697710771 1106 10-11 0.4218001740629518 1106 12-13 1.4355323143352408 1106 14-15 -0.6246666317265479 1106 16-17 0.5043323661399626 1106 18-19 0.7602478422930048 1106 20-21 0.07174182091408454 1106 22-23 0.5155755715855008 1106 24-25 -1.7530815859912323 1106 26-27 0.4350061124287592 1106 28-29 -0.20635940822526777 1106 30-31 0.04229179799754945 1106 32-33 -0.003566418747482203 1106 34-35 0.9018631012759428 1106 36-37 1.0245857269601117 1106 38-39 -1.7112738334198951 1106 40-41 1.1489825899640635 1106 42-43 0.7382529854125437 1106 44-45 1.4185883950349378 1106 46-47 1.075218076688472 1106 48-49 1.0652956345091624 1106 50-51 -1.117243719892798 1106 52-53 -1.514097021394143 1106 54-55 -1.1341210666601498 1106 56-57 0.37048994416312553 1106 58-59 0.31634638933033443 1106 60-61 0.1903973567410091 1106 62-63 0.3194772129477883 1106 64-65 -1.7590231636551366 1106 66-67 -0.2592844909082359 1106 68-69 0.7950191790358403 1106 70-71 0.9504643997269433 1106 72-73 -1.238168348056032 1106 74-75 -3.836163370243071 1106 76 -1.7302963101312585 1107 1 1.2300277624183096 1107 2 0.30705144623744474 1107 3 0.7163119483164664 1107 4 0.4700315138472462 1107 5 0.2810873356064221 1107 6 0.49765093925427806 1107 7 0.9123586855506431 1107 8 0.33964353778358003 1107 9 -0.8106243073297605 1107 10-11 0.7827156670206961 1107 12-13 0.03447597630707122 1107 14-15 0.7673521476161866 1107 16-17 0.7738159342150794 1107 18-19 0.244402771870476 1107 20-21 0.8662253889892 1107 22-23 0.6122891866089759 1107 24-25 1.3571296816144027 1107 26-27 0.6035507133677243 1107 28-29 1.6146499814460924 1107 30-31 1.020343441190036 1107 32-33 1.482114332426221 1107 34-35 1.0082011294449487 1107 36-37 1.920829858415516 1107 38-39 0.4475289834815115 1107 40-41 0.014475547710546266 1107 42-43 1.2906004032529168 1107 44-45 1.3019217283682707 1107 46-47 1.7925889687072498 1107 48-49 2.044403615729827 1107 50-51 2.413859566492178 1107 52-53 1.7782738706246377 1107 54-55 0.22773339343374488 1107 56-57 1.7307012117687606 1107 58-59 1.3435764362786955 1107 60-61 1.4950921924217582 1107 62-63 1.6874349594266604 1107 64-65 1.6764228457345354 1107 66-67 -0.38757087588476224 1107 68-69 2.591850164951339 1107 70-71 0.3228822401025333 1107 72-73 0.7177001965449072 1107 74-75 -0.848722055689084 1107 76 0.7481074457372863 1108 1 -1.861419471711102 1108 2 0.11680853952179149 1108 3 0.005758774199300376 1108 4 0.4398212668580612 1108 5 0.37396343276910216 1108 6 0.3280244848758329 1108 7 0.4371596653424348 1108 8 -1.0351268235146378 1108 9 -0.28623157411890077 1108 10-11 -0.022040405647189232 1108 12-13 1.6380685462193014 1108 14-15 1.1144638030560614 1108 16-17 0.8985352646906932 1108 18-19 1.2185811756263334 1108 20-21 1.1141331252619082 1108 22-23 -0.617757761747832 1108 24-25 1.2422082690812388 1108 26-27 0.12631046025484238 1108 28-29 -0.6357072343122283 1108 30-31 0.5600454211859613 1108 32-33 1.160926334875704 1108 34-35 0.6112833911310105 1108 36-37 1.679658190728233 1108 38-39 2.7050305144061895 1108 40-41 2.0337651986597223 1108 42-43 0.29549936222414175 1108 44-45 1.6714869457595753 1108 46-47 1.370870250601513 1108 48-49 1.2196434605961244 1108 50-51 1.7468867148898113 1108 52-53 1.2840913844029629 1108 54-55 0.3147919768181069 1108 56-57 0.8404174803950113 1108 58-59 1.4380855197651172 1108 60-61 1.7222814147120218 1108 62-63 1.4988250390347417 1108 64-65 1.2880782856202302 1108 66-67 2.02730971199032 1108 68-69 2.525816280485124 1108 70-71 1.2363339649443361 1108 72-73 2.0198026664367212 1108 74-75 1.1670974993221463 1108 76 3.7870949942165666 1109 1 -1.033961579035818 1109 2 0.43799929549515326 1109 3 0.5412072661695362 1109 4 0.1474201701091431 1109 5 0.11740252403428286 1109 6 0.011925777472775678 1109 7 -0.3013832253743658 1109 8 0.431383164734477 1109 9 0.7599885590577529 1109 10-11 0.3305839578467413 1109 12-13 0.46053231433523933 1109 14-15 1.0825405754806638 1109 16-17 1.1399179517255504 1109 18-19 0.9798424368876013 1109 20-21 1.1242192983915587 1109 22-23 0.8597197157296392 1109 24-25 -0.6240275319371733 1109 26-27 -0.26274163531898864 1109 28-29 -2.437215264081125 1109 30-31 0.08981432052006966 1109 32-33 0.6847218695408017 1109 34-35 0.4856468850597295 1109 36-37 2.079090231464619 1109 38-39 1.0772396800936193 1109 40-41 0.31519880618028395 1109 42-43 0.7499646971242555 1109 44-45 0.849219025665569 1109 46-47 1.2202631217335167 1109 48-49 1.2013316705452084 1109 50-51 1.2284770008279224 1109 52-53 -0.21297089526801471 1109 54-55 -1.1001120576511383 1109 56-57 0.3981926468658301 1109 58-59 -1.28185180886787 1109 60-61 1.2381451044887584 1109 62-63 0.5525853210559006 1109 64-65 1.5472831426511746 1109 66-67 0.9274272208034802 1109 68-69 -0.15025109123442704 1109 70-71 1.8560950303575758 1109 72-73 0.950570390682703 1109 74-75 0.6190168099371114 1109 76 0.024658644823695397 1110 1 -0.49837599345023165 1110 2 -0.2795682720724102 1110 3 0.4970631220253878 1110 4 0.26228503497400624 1110 5 0.3101953168270768 1110 6 0.24976361531061286 1110 7 0.7567248827337423 1110 8 0.6858876692389799 1110 9 1.06449306356226 1110 10-11 0.4384668407296175 1110 12-13 1.352198981001905 1110 14-15 0.9086667016067906 1110 16-17 1.2043323661399654 1110 18-19 -0.023085491040326644 1110 20-21 0.8134084875807517 1110 22-23 -0.13442442841450486 1110 24-25 -0.7614149193245581 1110 26-27 1.5266727790954207 1110 28-29 0.8769739251080608 1110 30-31 0.35895846466421233 1110 32-33 1.5797669145858464 1110 34-35 0.8101964346092743 1110 36-37 1.8912523936267789 1110 38-39 1.7970594999134377 1110 40-41 -0.9676840767025965 1110 42-43 0.8132529854125394 1110 44-45 1.5769217283682764 1110 46-47 1.0585514100218063 1110 48-49 1.973628967842501 1110 50-51 -0.5089103865594637 1110 52-53 0.4775696452725242 1110 54-55 0.6075456000065174 1110 56-57 1.2454899441631255 1110 58-59 -0.33365361066967125 1110 60-61 0.6820640234076834 1110 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58-59 -1.9535723098566535 2116 60-61 -3.525862805860612 2116 62-63 -6.447382717240657E-4 2116 64-65 -4.383006903492532 2116 66-67 -2.3411950600139235 2116 68-69 -2.953151552671475 2116 70-71 -1.764372998647037 2116 72-73 -1.8999569659422129 2116 74-75 -3.351813776747136 2116 76 -2.9994020011881695 2117 1 1.231111186036948 2117 2 0.8704317279275884 2117 3 0.3457810707433353 2117 4 -0.15438163169265806 2117 5 0.1409645475963046 2117 6 0.003609769156767584 2117 7 0.9221094981183526 2117 8 0.39742613077744693 2117 9 -1.581660782591591 2117 10-11 -1.7544818772190993 2117 12-13 -2.6997240959211695 2117 14-15 -0.7400512471111611 2117 16-17 -1.2213086595010623 2117 18-19 -0.8711624141172507 2117 20-21 0.5377674619397297 2117 22-23 -0.6946808386709051 2117 24-25 -0.934491842401485 2117 26-27 -0.9444810670584189 2117 28-29 -0.908282485148348 2117 30-31 -1.0865543558486053 2117 32-33 -3.263822829003896 2117 34-35 -1.073777924365082 2117 36-37 -2.3164399140655263 2117 38-39 -1.1606328077788746 2117 40-41 -0.593325102343627 2117 42-43 -1.673285476125919 2117 44-45 -0.7237192972727513 2117 46-47 -2.029910128439731 2117 48-49 -1.7513710321575005 2117 50-51 -1.030705258354331 2117 52-53 0.7634670811699635 2117 54-55 -0.8206595281986111 2117 56-57 -0.13784338917020023 2117 58-59 -2.558012585028635 2117 60-61 -0.5352436689000157 2117 62-63 -0.8952663767958029 2117 64-65 -1.778253932885903 2117 66-67 -1.7458229524466944 2117 68-69 -1.2261346671180036 2117 70-71 -0.31940739514485017 2117 72-73 0.05157524168755501 2117 74-75 1.8042212451415445 2117 76 0.5184216385866911 2118 1 0.5200924750182381 2118 2 0.4650263225221849 2118 3 -1.269603544641278 2118 4 0.18796071064968345 2118 5 0.3065917132234688 2118 6 0.22814199368899324 2118 7 0.9283465043553605 2118 8 0.0800318133831297 2118 9 -1.1589303598611664 2118 10-11 -2.3248214475586693 2118 12-13 -1.7759541721512448 2118 14-15 -1.6336756407355537 2118 16-17 -4.556027994220397 2118 18-19 -0.7769143198691566 2118 20-21 -1.3555104313381676 2118 22-23 0.4272872832972112 2118 24-25 0.2948913869817389 2118 26-27 -3.066795689373045 2118 28-29 -1.0791071559730199 2118 30-31 -0.8155910848853338 2118 32-33 -0.09230515748622281 2118 34-35 1.262673912086747 2118 36-37 -5.306044903670518 2118 38-39 1.5434558963098297 2118 40-41 0.7206042115856874 2118 42-43 -0.8378731407135831 2118 44-45 -4.73861881217227 2118 46-47 -0.6986557971854026 2118 48-49 -0.5756953564818232 2118 50-51 -2.4471986748477477 2118 52-53 -2.32107900337612 2118 54-55 -1.2149769225160085 2118 56-57 -0.33153708286390327 2118 58-59 -2.0521220791381367 2118 60-61 -1.4105035441598872 2118 62-63 -1.7514687329981626 2118 64-65 -0.3783925330245026 2118 66-67 0.5895893829656416 2118 68-69 -1.1907916317749674 2118 70-71 0.6060950303575758 2118 72-73 2.8492190393313557 2118 74-75 2.328476269396571 2118 76 2.2543883745534252 2119 1 1.2881831463347169 2119 2 -1.6456973043304757 2119 3 0.14975129406840182 2119 4 -2.294166577929218 2119 5 -0.35862188747400126 2119 6 -0.24701057823777006 2119 7 -0.43521060113722854 2119 8 0.2369629380561875 2119 9 0.08169736463752741 2119 10-11 0.006477593417784533 2119 12-13 0.38795166917394397 2119 14-15 0.3484516478433477 2119 16-17 -1.061259031709497 2119 18-19 -0.08249409319086709 2119 20-21 -0.4527205446773124 2119 22-23 -0.25754270798439904 2119 24-25 0.9993377688474823 2119 26-27 0.9070491231814515 2119 28-29 -0.07867123618225946 2119 30-31 0.31997997004056344 2119 32-33 -0.09012555853243498 2119 34-35 -0.08039496324018813 2119 36-37 -2.2668121225022517 2119 38-39 -3.1540157689037684 2119 40-41 -1.9171464422939977 2119 42-43 0.47642502842330003 2119 44-45 -0.5529169813091528 2119 46-47 0.22898151754868934 2119 48-49 -1.2191129676413723 2119 50-51 -0.6481576983874149 2119 52-53 -0.8659787418242502 2119 54-55 -1.2515941849397194 2119 56-57 -3.143219733256224 2119 58-59 0.1797872495453845 2119 60-61 -0.8751940411084504 2119 62-63 -0.8216518193102758 2119 64-65 -0.33698015290244143 2119 66-67 -0.5477253511232831 2119 68-69 0.007115953229391181 2119 70-71 -1.3363635572623025 2119 72-73 -1.6241898534323767 2119 74-75 -1.0477225100280165 2119 76 -0.032446847765665865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 4.0 23 18.0 24 27.0 25 71.0 26 141.0 27 255.0 28 420.0 29 657.0 30 1042.0 31 1445.0 32 2134.0 33 2861.0 34 3682.0 35 3356.0 36 2529.0 37 2466.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.703775640721965 31.773177317731772 10.564214316168458 40.9588327253778 2 8.541380453834858 11.307972902553413 65.85816476384481 14.292481879766925 3 8.214505661092424 35.3356388270406 44.81974513240798 11.630110379458998 4 5.831635795158463 27.45274527452745 32.73485243261168 33.980766497702405 5 5.391065422331707 53.16689563693211 32.42218958737979 9.019849353356388 6 11.01425932066891 35.75725993651997 12.27438533327017 40.954095409540955 7 10.535790421147379 52.328390733810224 12.18437633237008 24.95144251267232 8 9.318300251077739 33.71547681083898 30.366194514188262 26.600028423895022 9 10.459993367757829 24.420863138945474 12.397555545028187 52.721587948268514 10-11 10.490785920697334 20.96972855180255 37.25662039888199 31.282865128618127 12-13 20.851295655881376 11.426405798474585 28.3575725993652 39.36472594627884 14-15 27.230091430195653 22.343550144488134 13.899284665308636 36.527073760007575 16-17 26.043393813065517 25.512814439338673 11.322184850063955 37.121606897531855 18-19 19.636174143730162 26.206831209436732 11.158747453692738 42.998247193140365 20-21 21.24923018617651 20.315978966317687 11.56141929982472 46.87337154768109 22-23 27.933582831967406 32.76090766971434 11.594580510682647 27.710928987635608 24-25 25.882325074612723 38.381732910133124 11.81012838125918 23.925813633994977 26-27 30.018001800180016 28.37889052063101 23.613150788763086 17.989956890425884 28-29 30.063006300630065 15.130986782888813 35.79278980529632 19.0132171111848 30-31 31.59315931593159 22.319863565303898 22.954663887441377 23.13231323132313 32-33 13.470557582073997 30.61490359562272 20.75654933914444 35.15798948315884 34-35 9.794400492680847 40.01136955800843 23.281538680183807 26.912691269126913 36-37 9.673598938841252 35.62461509308826 29.631910559477 25.06987540859349 38-39 19.98673551565683 17.400161068738456 27.748827514330383 34.86427590127434 40-41 17.196456487754038 22.374342697427636 33.909706760149696 26.519494054668623 42-43 28.303093467241464 19.34956653560093 38.74176891373347 13.605571083424131 44-45 17.871523994504717 20.289923729215026 29.205552134160783 32.63300014211947 46-47 29.643753849069114 12.786015443649628 26.199725235681466 31.37050547159979 48-49 23.279170022265387 11.56852527357999 31.117059074328484 34.035245629826136 50-51 11.876450802975034 11.641953669051116 40.86645506655929 35.61514046141456 52-53 19.427732246908903 11.893031408403997 47.46790468520536 21.21133165948174 54-55 16.0192335022976 12.200956937799043 40.89014164574352 30.889667914159837 56-57 28.02122317494907 12.380974939599223 34.43791747595812 25.159884409493582 58-59 17.404898384575297 34.016296366478755 34.31000994836326 14.26879530058269 60-61 17.29594012032782 55.06182197167085 16.481121796390166 11.161116111611161 62-63 9.477000331612109 67.16329527689611 11.900137382159269 11.459567009332511 64-65 8.126865318110758 68.47316310578427 11.606423800274765 11.793547775830215 66-67 8.458477426690038 66.8174712208063 11.587474536927376 13.136576815576294 68-69 9.105121038419632 59.88914680941778 12.89260504997868 18.113127102183903 70-71 9.898621441091478 53.65720782604576 14.659623857122556 21.784546875740205 72-73 10.573688947842152 47.093656734094466 16.15187834572931 26.180775972334075 74-75 11.151641479937467 45.686673930550945 16.343739637121608 26.817944952389976 76 10.393670946041974 47.690558529537164 15.339428679710077 26.57634184471079 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 2.0 15 1.0 16 0.5 17 0.0 18 3.0 19 5.0 20 3.5 21 3.0 22 6.0 23 11.5 24 19.5 25 25.0 26 37.0 27 47.0 28 61.0 29 77.0 30 82.5 31 96.5 32 133.0 33 161.0 34 183.5 35 225.0 36 282.5 37 321.0 38 351.0 39 660.0 40 1606.5 41 2830.5 42 3387.0 43 3426.0 44 3089.5 45 2247.5 46 1781.0 47 1506.0 48 1020.5 49 714.5 50 619.0 51 542.5 52 414.0 53 334.0 54 306.0 55 287.0 56 231.5 57 169.0 58 143.0 59 138.5 60 113.0 61 84.5 62 77.0 63 64.5 64 40.5 65 17.0 66 5.5 67 6.0 68 5.0 69 4.0 70 3.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 21109.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.85934909280402 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83188405797101 31.801601212752857 2 13.692753623188405 11.189540006632242 3 4.3826086956521735 5.372116158984319 4 1.5420289855072464 2.5202520252025202 5 0.7072463768115942 1.444881330238287 6 0.35942028985507246 0.8811407456535127 7 0.2202898550724638 0.6300630063006301 8 0.12753623188405797 0.4168837936425221 9 0.11594202898550725 0.42635842531621587 >10 0.7884057971014493 5.8505850585058505 >50 0.10434782608695652 2.984508977213511 >100 0.0927536231884058 7.3428395471126064 >500 0.0 0.0 >1k 0.034782608695652174 29.13922971244493 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT 3186 15.09308825619404 Illumina PCR Primer Index 1 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 1643 7.783409919939363 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 1322 6.262731536311526 No Hit CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 475 2.25022502250225 No Hit TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 287 1.3596096451750437 Illumina PCR Primer Index 1 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 189 0.8953526931640532 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 138 0.6537495854848643 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT 124 0.5874271637690085 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 117 0.5542659529110806 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT 113 0.5353166895636932 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 107 0.5068927945426122 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 100 0.47373158368468427 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 86 0.4074091619688284 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT 84 0.39793453029513476 No Hit CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 69 0.32687479274243214 Illumina PCR Primer Index 1 (95% over 21bp) CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 66 0.3126628452318916 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT 58 0.27476431853711686 No Hit TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 57 0.27002700270027 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 56 0.2652896868634232 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 54 0.2558150551897295 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT 48 0.22739116016864844 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT 43 0.2037045809844142 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 40 0.1894926334738737 No Hit ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT 35 0.1658060542896395 Illumina PCR Primer Index 1 (95% over 24bp) CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC 34 0.16106873845279263 No Hit CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT 32 0.15159410677909896 No Hit CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT 31 0.14685679094225212 No Hit ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT 29 0.13738215926855843 TruSeq Adapter, Index 1 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT 27 0.12790752759486476 Illumina PCR Primer Index 1 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCCT 27 0.12790752759486476 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG 25 0.11843289592117107 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT 25 0.11843289592117107 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT 25 0.11843289592117107 No Hit TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 25 0.11843289592117107 No Hit CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 25 0.11843289592117107 No Hit ATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGTCTT 24 0.11369558008432422 Illumina PCR Primer Index 1 (95% over 22bp) TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 24 0.11369558008432422 No Hit ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT 23 0.10895826424747738 TruSeq Adapter, Index 4 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT 22 0.10422094841063052 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0047373158368468424 0.0 2 0.0 0.0 0.0 0.0047373158368468424 0.0 3 0.0 0.0 0.0 0.0047373158368468424 0.0 4 0.0 0.0 0.0 0.0047373158368468424 0.0 5 0.0 0.0 0.0 0.009474631673693685 0.0 6 0.0 0.0 0.0 0.009474631673693685 0.0 7 0.0 0.0 0.0 0.009474631673693685 0.0 8 0.0 0.0 0.0 0.009474631673693685 0.0 9 0.0 0.0 0.0 0.009474631673693685 0.0 10 0.0 0.0 0.0 0.009474631673693685 0.0 11 0.0 0.0 0.0 0.009474631673693685 0.0 12 0.0 0.0 0.0 0.009474631673693685 0.0 13 0.0 0.0 0.0 0.009474631673693685 0.0 14 0.0 0.0 0.0 0.009474631673693685 0.0 15 0.0 0.0 0.0 0.009474631673693685 0.0 16 0.0 0.0 0.0 0.023686579184234214 0.0 17 0.0 0.0 0.0 0.023686579184234214 0.0 18 0.0 0.0 0.0 0.03789852669477474 0.0 19 0.0 0.0 0.0 0.03789852669477474 0.0 20 0.0 0.0 0.0 0.04263584253162158 0.0 21 0.0 0.0 0.0 0.04263584253162158 0.0 22 0.0 0.0 0.0 0.04263584253162158 0.0 23 0.0 0.0 0.0 0.04263584253162158 0.0 24 0.0 0.0 0.0 0.04737315836846843 0.0 25 0.0 0.0 0.0 0.04737315836846843 0.0 26 0.0 0.0 0.0 0.04737315836846843 0.0 27 0.0 0.0 0.0 0.05684779004216211 0.0 28 0.0 0.0 0.0 0.0663224217158558 0.0 29 0.0 0.0 0.0 0.07105973755270265 0.0 30 0.0 0.0 0.0 0.08053436922639633 0.0 31 0.0 0.0 0.0 0.09474631673693686 0.0 32 0.0 0.0 0.0 0.1326448434317116 0.0 33 0.0 0.0 0.0 0.16106873845279265 0.0 34 0.0 0.0 0.0 0.1894926334738737 0.0 35 0.0 0.0 0.0 0.2226538443318016 0.0 36 0.0 0.0 0.0 0.3031882135581979 0.0 37 0.0 0.0 0.0 0.421621109479369 0.0 38 0.0 0.0 0.0 0.5590032687479274 0.0 39 0.0 0.0 0.0 0.724809323037567 0.0 40 0.0 0.0 0.0 0.8337675872850443 0.0 41 0.0 0.0 0.0 0.994836325737837 0.0 42 0.0 0.0 0.0 1.269600644274954 0.0 43 0.0 0.0 0.0 1.4733052252593681 0.0 44 0.0 0.0 0.0 1.7291202804490975 0.0 45 0.0 0.0 0.0 2.0370458098441424 0.0 46 0.0 0.0 0.0 2.2075891799706286 0.0 47 0.0 0.0 0.0 2.449192287649818 0.0 48 0.0 0.0 0.0 2.709744658676394 0.0 49 0.0 0.0 0.0 2.918186555497655 0.0 50 0.0 0.0 0.0 3.098204557297835 0.0 51 0.0 0.0 0.0 3.2924345066085556 0.0 52 0.0 0.0 0.0 3.519825666777204 0.0 53 0.0 0.0 0.0 3.714055616087925 0.0 54 0.0 0.0 0.0 3.8561750911933297 0.0 55 0.0 0.0 0.0 4.078828935525132 0.0 56 0.0 0.0 0.0 4.3156947273674735 0.0 57 0.0 0.0 0.0 4.467288834146572 0.0 58 0.0 0.0 0.0 4.656781467620446 0.0 59 0.0 0.0 0.0 4.832062153583779 0.0 60 0.0 0.0 0.0 4.9504950495049505 0.0 61 0.0 0.0 0.0 5.135250367141977 0.0 62 0.0 0.0 0.0 5.3105310531053105 0.0 63 0.0 0.0 0.0 5.466862475721256 0.0 64 0.0 0.0 0.0 5.698990951726752 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCGTC 15 0.0021946765 70.0 27 ATTACAC 15 0.0021946765 70.0 4 TCGTCAC 15 0.0021946765 70.0 29 AAAACCT 15 0.0021946765 70.0 67 AGACTCG 15 0.0021946765 70.0 25 TTAACAC 35 3.4833647E-9 70.0 2 CTCGTCA 15 0.0021946765 70.0 28 GAGACTC 15 0.0021946765 70.0 24 TCTTAAT 20 7.7499215E-5 70.0 1 TTTACAC 165 0.0 67.878784 2 CTTTACA 180 0.0 62.222218 1 CTTAACA 40 1.0013537E-8 61.25 1 TTACACA 190 0.0 58.947372 3 TCTTTAT 30 8.099712E-6 58.333332 1 TAACACA 45 2.5374902E-8 54.444443 3 CGTCACA 20 0.0068583055 52.5 30 TTGACAA 20 0.0068583055 52.5 58 CTTGACA 20 0.0068583055 52.5 57 CTTGAAC 20 0.0068583055 52.5 57 AAAAATT 20 0.0068583055 52.5 63 >>END_MODULE