FastQCFastQC Report
Wed 25 May 2016
SRR1780391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129499
Sequences flagged as poor quality0
Sequence length50
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT1580.1220086641595688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTAT207.866232E-443.9594421
GCACTCT250.00234232735.20835510
CTTAATA250.002355697835.1675572
CTGACGT250.002355697835.1675579
CTATACA551.368735E-731.9705051
ATGCACA353.2195004E-431.39964
TTCGTCC502.3569082E-630.7716122
CTTTAGT502.3569082E-630.7716128
CGTCCTT502.3569082E-630.7716124
GCGACAA300.00570885729.35164316
GACAATG300.00570885729.35164318
ATTTCGG300.00574133229.31762142
CTTATAG300.005752189629.3062971
ATAACAC300.005752189629.3062974
CTTTACA602.9347757E-729.3062971
CCTTTAG554.9516766E-627.9741927
TCCTTTA554.9516766E-627.9741926
TTTATAC407.035861E-427.4746513
TTATACA3950.026.7095362
CGGTGGT858.138159E-925.86848843
Produced by FastQC (version 0.11.5)