FastQCFastQC Report
Wed 25 May 2016
SRR1780391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129499
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT2898022.378551185723442RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC112808.710491972910987No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT100367.74986679433818No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC44523.4378643850531665No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT25881.9984710306643294No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG13081.0100464096247848No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC12290.9490420775450004No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC10550.8146781056224373No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG8590.6633255855257569No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC8530.658692345114634No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG7380.5698885705681125No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5410.4177638437362451No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5350.41313060332512214No Hit
CTTATCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5320.41081398311956074No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5040.38919219453432075No Hit
CTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT4910.3791535069768879No Hit
ACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCT3460.26718353037475195RNA PCR Primer, Index 32 (95% over 23bp)
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3090.23861188117282758No Hit
CTTATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC2950.22780098688020756No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTCTTC2420.18687402991528893TruSeq Adapter, Index 10 (95% over 23bp)
TCTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT2170.16756886153561032No Hit
ATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCTGCTT1510.11660321701325879TruSeq Adapter, Index 8 (96% over 26bp)
CTTATACCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG1330.10270349577989019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC351.4298348E-744.0115934
AGCGGAT207.835329E-443.99459535
TTTACAC12300.042.759232
CCGTCTT30700.042.56154344
CTTTACA12400.042.463621
CTTATAA1000.041.859541
CTATACA4600.039.273251
GCCGTCT33850.038.53588543
TTACACA13800.038.270953
CTTATAC12300.038.1518251
TCTTTAT1350.037.5348661
TTATCAC1000.037.4098552
GCTCTGA250.002342033235.20927411
AAAGAAT250.002342033235.2092743
CGTGAAG250.002346479335.19567526
CGCATAA250.002346479335.19567541
TTACTCG250.002346479335.19567519
CTTATCA1100.034.0484241
TCACATC1100.033.9958235
TTATAAC1350.032.601182
Produced by FastQC (version 0.11.5)