FastQCFastQC Report
Wed 25 May 2016
SRR1780390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48927
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACAT150.0022130569.9928432
CGATGCC150.0022130569.9928416
AACCGTA200.006915292752.49463358
GGTCGTG200.006915292752.49463353
TCGTGGT200.006915292752.49463355
GTCGTGG200.006915292752.49463354
CGAATGT200.006915292752.49463329
CTTTACC200.006915292752.49463359
GACTAGG200.006915292752.4946332
GAAACCC404.3443473E-544.0608941
CGACGAA400.002398130534.9964222
AGCAATA500.00714767627.99713749
AAACCCA1355.8379774E-620.738622
AACCCAC1000.00956785617.498213
ATACACA5300.00117544347.2634084
Produced by FastQC (version 0.11.5)