##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780390_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48927 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.106975698489588 34.0 23.0 34.0 12.0 34.0 2 29.138941688638177 34.0 24.0 34.0 12.0 34.0 3 29.288572771680258 34.0 27.0 34.0 11.0 34.0 4 29.55122938254951 34.0 31.0 34.0 11.0 34.0 5 29.454350358697653 34.0 27.0 34.0 11.0 34.0 6 31.945428904285976 37.0 30.0 38.0 11.0 38.0 7 31.312158930651787 37.0 27.0 38.0 11.0 38.0 8 30.9782533161649 36.0 27.0 38.0 11.0 38.0 9 31.482984854988043 37.0 27.0 38.0 12.0 38.0 10-11 31.536983669548512 37.0 27.0 38.0 11.0 38.0 12-13 31.69644572526417 37.0 27.5 38.0 11.0 38.0 14-15 31.43192920064586 37.0 27.0 38.0 11.0 38.0 16-17 31.266407096286304 37.0 27.0 38.0 11.0 38.0 18-19 31.288327508328734 37.0 26.5 38.0 11.0 38.0 20-21 31.385594865820508 37.0 27.0 38.0 11.0 38.0 22-23 31.157499948903467 37.0 26.5 38.0 11.0 38.0 24-25 31.56303268134159 37.0 27.0 38.0 11.0 38.0 26-27 31.491528195066117 37.0 27.0 38.0 11.0 38.0 28-29 31.499039385206533 37.0 27.0 38.0 11.0 38.0 30-31 31.41490383632759 37.0 27.0 38.0 11.0 38.0 32-33 31.200012263167576 37.0 26.5 38.0 11.0 38.0 34-35 31.338340793426944 37.0 26.5 38.0 11.0 38.0 36-37 31.293447380791793 37.0 26.5 38.0 11.0 38.0 38-39 31.470445766141395 37.0 27.0 38.0 11.0 38.0 40-41 31.17488298894271 37.0 26.0 38.0 11.0 38.0 42-43 31.42724875835428 37.0 27.0 38.0 11.0 38.0 44-45 30.701821080385063 37.0 25.5 38.0 11.0 38.0 46-47 31.278803114844564 37.0 27.0 38.0 11.0 38.0 48-49 31.266499070043125 37.0 26.5 38.0 11.0 38.0 50-51 31.089071473828355 37.0 26.5 38.0 11.0 38.0 52-53 31.09512130316594 37.0 26.0 38.0 11.0 38.0 54-55 30.884828417846997 37.0 26.0 38.0 11.0 38.0 56-57 30.79502115396407 37.0 25.5 38.0 11.0 38.0 58-59 30.749586118094307 37.0 25.5 38.0 11.0 38.0 60-61 30.837850266723894 37.0 25.5 38.0 11.0 38.0 62-63 30.80548572362908 37.0 25.5 38.0 11.0 38.0 64-65 30.938183416109716 37.0 25.5 38.0 11.0 38.0 66-67 30.898011322991394 37.0 25.0 38.0 11.0 38.0 68-69 30.335745089623316 36.5 24.5 38.0 11.0 38.0 70-71 30.48435424203405 36.5 25.0 38.0 11.0 38.0 72-73 30.657346659308764 37.0 25.0 38.0 11.0 38.0 74-75 30.26043902139923 36.0 24.0 38.0 11.0 38.0 76 29.572526416906822 35.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.2269855028865067 1101 2 -0.24236456988548483 1101 3 0.11724852328833535 1101 4 -1.4427648196213276 1101 5 0.03679355245299476 1101 6 -2.5747902050297817 1101 7 -1.2248126286090262 1101 8 -1.4473772810123506 1101 9 -0.9546315825451934 1101 10-11 -1.5600322694598319 1101 12-13 -0.6159793613140501 1101 14-15 -0.8986970885459762 1101 16-17 -2.011123217746686 1101 18-19 -0.8648148320672284 1101 20-21 -0.6027480819176958 1101 22-23 -0.14208211185602337 1101 24-25 -1.2898428985317985 1101 26-27 -0.3010916581676817 1101 28-29 -0.1551814732107637 1101 30-31 -0.6756125063401228 1101 32-33 -0.44627008052274775 1101 34-35 -1.2317655605742601 1101 36-37 -0.6667338292292264 1101 38-39 -1.1486097983908259 1101 40-41 0.43652990053396223 1101 42-43 -0.2663509631130836 1101 44-45 -0.2742166485594524 1101 46-47 -0.11073282466759693 1101 48-49 -0.6303215772440254 1101 50-51 0.10794071788655657 1101 52-53 0.027148787631674054 1101 54-55 -1.441619170117793 1101 56-57 -0.6752042110573946 1101 58-59 -0.42304248470712835 1101 60-61 -0.9442787747106287 1101 62-63 -1.1669270649018095 1101 64-65 -0.14449303237732636 1101 66-67 -0.594951338150949 1101 68-69 -0.20999688821551388 1101 70-71 0.03245920058926899 1101 72-73 -1.1739084685568635 1101 74-75 -1.0517283460069429 1101 76 -2.430401428740229 1102 1 0.24368176088420412 1102 2 -0.022391439567527982 1102 3 -0.16000181258268853 1102 4 -0.7827110802572435 1102 5 -0.6100044771036544 1102 6 0.8023705652344333 1102 7 0.5310315272351325 1102 8 0.9427257194354794 1102 9 1.0224396221122163 1102 10-11 0.8383331582159386 1102 12-13 -2.7360868400422156 1102 14-15 -0.8428753241035238 1102 16-17 -1.4943968406754777 1102 18-19 -1.1819890371724675 1102 20-21 -1.1687579341344474 1102 22-23 -0.7450825596840538 1102 24-25 -0.20130729620309396 1102 26-27 -4.18145439887525 1102 28-29 -0.5629960724046725 1102 30-31 -0.6691861740517204 1102 32-33 -4.571953018274652 1102 34-35 -0.6054108807713021 1102 36-37 0.4569786651729224 1102 38-39 -0.33333886243023514 1102 40-41 -0.29125782897790486 1102 42-43 -0.11037245885871627 1102 44-45 -4.764901825451528 1102 46-47 -0.49700689542442333 1102 48-49 -0.37353698252839607 1102 50-51 -5.123810289726521 1102 52-53 -2.0384132365510403 1102 54-55 -0.9490978982861797 1102 56-57 -1.3750698626471838 1102 58-59 -0.250091297962836 1102 60-61 -0.02759718044282522 1102 62-63 -3.4323099578709098 1102 64-65 -1.289253444379117 1102 66-67 -1.253057025566985 1102 68-69 -6.651824026594376 1102 70-71 -0.7159957926933096 1102 72-73 -0.10698056887034468 1102 74-75 -5.408244243902153 1102 76 -2.1404327767026103 1103 1 -1.2620653655525764 1103 2 -1.8706673016364945 1103 3 -1.0404615826976311 1103 4 -1.4562742986480472 1103 5 -0.9927630977933113 1103 6 -2.2700432278690137 1103 7 -0.5977041049487752 1103 8 -0.4929064644725685 1103 9 -1.2442270445544494 1103 10-11 -1.4743105199449786 1103 12-13 -2.4194201733755527 1103 14-15 -2.1457488873219184 1103 16-17 -1.5765807487214545 1103 18-19 -1.6791154739540772 1103 20-21 -1.7020912674677788 1103 22-23 -1.8973814102587703 1103 24-25 -0.37142223873183156 1103 26-27 -2.4670865827832955 1103 28-29 -1.3118466471173171 1103 30-31 -2.2657378981896485 1103 32-33 -5.177125432067754 1103 34-35 -1.5002384669781996 1103 36-37 -1.2412971968960385 1103 38-39 -1.459775644039432 1103 40-41 -0.33321185196641423 1103 42-43 -1.0322115393184852 1103 44-45 -5.212602974876816 1103 46-47 -1.7562022977232736 1103 48-49 -1.3631921549421868 1103 50-51 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1104 32-33 -1.0582830675357364 1104 34-35 0.05692491561621793 1104 36-37 -0.34043512793052244 1104 38-39 -0.47896694124796824 1104 40-41 0.49334808235214567 1104 42-43 0.24447154770943058 1104 44-45 -2.200082449425256 1104 46-47 -0.16619819263296165 1104 48-49 0.5133472539248025 1104 50-51 0.6571831421289787 1104 52-53 -0.11597891799603133 1104 54-55 -0.3409698194684445 1104 56-57 0.14405985820667766 1104 58-59 0.49984712568248213 1104 60-61 0.4318034763716234 1104 62-63 0.33090843293368977 1104 64-65 0.26513900225470977 1104 66-67 -0.42070891390852694 1104 68-69 -3.060916801635429 1104 70-71 -0.7305278123977423 1104 72-73 -0.35735002699842155 1104 74-75 -0.39317856245716243 1104 76 -0.5862455845843826 1105 1 -0.7152404173709286 1105 2 -0.019527297867885096 1105 3 -0.9004578140901316 1105 4 -0.8474557570991514 1105 5 -0.1673438402089893 1105 6 -0.8570980611081289 1105 7 0.012998740349885907 1105 8 -0.40632270717089014 1105 9 -0.9331284954683383 1105 10-11 -0.7340617918500101 1105 12-13 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-1.3735211505484735 1106 1 -0.22172279174144194 1106 2 0.22015983381538717 1106 3 -0.808097372645797 1106 4 0.419768072323329 1106 5 0.22867030315327597 1106 6 -0.7819882357572254 1106 7 -0.06896847276486895 1106 8 -0.20949424901166935 1106 9 0.23830619862788183 1106 10-11 -0.543501414693683 1106 12-13 -0.40640462232754615 1106 14-15 -0.38955100587048364 1106 16-17 -0.06164948886119248 1106 18-19 -1.2907336844566686 1106 20-21 -0.13075928639948842 1106 22-23 -0.2636762040383509 1106 24-25 0.04845605741421721 1106 26-27 -0.7677965219313663 1106 28-29 -0.8658809044984288 1106 30-31 0.05745344731406732 1106 32-33 0.28123607345399293 1106 34-35 -0.3916628528244921 1106 36-37 -0.04876846126385814 1106 38-39 -0.2541186686055781 1106 40-41 0.2110184838460718 1106 42-43 0.27948555331167313 1106 44-45 0.4417242732638229 1106 46-47 0.08969583164341444 1106 48-49 -0.38505377315269484 1106 50-51 0.2305958405416746 1106 52-53 -0.18781598615662887 1106 54-55 0.0906361562551794 1106 56-57 0.6396247508303894 1106 58-59 -0.23235408813637903 1106 60-61 0.8359818143734934 1106 62-63 0.36685614535199207 1106 64-65 0.8086730825534936 1106 66-67 0.7660324493034238 1106 68-69 0.08479981834589623 1106 70-71 0.06802494203736487 1106 72-73 -0.020165153048843365 1106 74-75 -1.6117826707671519 1106 76 -0.997543437058706 1107 1 -0.3576240950635601 1107 2 0.24583953370323286 1107 3 0.34014101541159647 1107 4 0.2524246063373283 1107 5 0.3127867899846919 1107 6 0.5643783187541231 1107 7 1.0257060834481457 1107 8 0.6062229648955899 1107 9 0.6915683713470671 1107 10-11 0.4492084897809292 1107 12-13 0.5912749228633096 1107 14-15 -0.24183472014514962 1107 16-17 0.8998696355538627 1107 18-19 1.0315388142864137 1107 20-21 1.5409543694770633 1107 22-23 0.8079678381939281 1107 24-25 0.6190558949823775 1107 26-27 0.08700101838653396 1107 28-29 -0.4950915631208517 1107 30-31 0.6445865253973722 1107 32-33 0.9464717929883584 1107 34-35 0.8360704495694407 1107 36-37 0.6070500791700688 1107 38-39 0.7753124332237107 1107 40-41 0.6882973638398866 1107 42-43 0.8123780002312628 1107 44-45 0.2814968686925212 1107 46-47 0.32723305560597993 1107 48-49 0.10110084648609075 1107 50-51 0.5991034013569312 1107 52-53 1.0765224908515307 1107 54-55 0.7594105693734825 1107 56-57 0.9962013065019697 1107 58-59 0.5229346151161245 1107 60-61 -0.11113115310146426 1107 62-63 -0.33279965385132115 1107 64-65 0.015174223443775503 1107 66-67 0.18932208073785262 1107 68-69 1.0943910315570236 1107 70-71 0.3246285696817175 1107 72-73 0.5489263888933777 1107 74-75 0.4679442890503047 1107 76 -1.3914019666638424 1108 1 -1.4296958360316232 1108 2 -1.0148095162434778 1108 3 -1.8017238087274734 1108 4 -1.4040861070295705 1108 5 -0.5998524102799259 1108 6 -1.134361068948472 1108 7 0.19190109245252174 1108 8 0.1254629222626349 1108 9 -1.9461617914667073 1108 10-11 -1.064408328183717 1108 12-13 -0.795821258547253 1108 14-15 -0.8197868683314091 1108 16-17 -2.1191130539973884 1108 18-19 -1.48805029225921 1108 20-21 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0.40868295337465455 1109 6 0.9071536575592418 1109 7 -1.7721942792164818 1109 8 0.4597446293353684 1109 9 -0.2095959729934549 1109 10-11 1.123650177125839 1109 12-13 0.4900533156863709 1109 14-15 0.1051780685549879 1109 16-17 0.6774607789018283 1109 18-19 0.14593087383976666 1109 20-21 0.42167588121594335 1109 22-23 0.3069752823626608 1109 24-25 0.572997486889232 1109 26-27 -0.17266685716223407 1109 28-29 0.42699280412480434 1109 30-31 0.23626432650445395 1109 32-33 1.0227077158744073 1109 34-35 -0.04167339467564091 1109 36-37 0.22972616239205834 1109 38-39 0.33618282833728586 1109 40-41 0.31074439571619905 1109 42-43 0.6400168626095848 1109 44-45 -0.15188736493984578 1109 46-47 -0.13760756283265252 1109 48-49 0.16951084839485375 1109 50-51 1.0159005000398835 1109 52-53 1.2103075643388408 1109 54-55 1.1039610561075968 1109 56-57 0.7656420394662753 1109 58-59 -0.459657482612446 1109 60-61 0.3016575470321463 1109 62-63 -0.571242104700719 1109 64-65 0.008188157400638829 1109 66-67 0.7454969232650512 1109 68-69 1.0344941046625742 1109 70-71 -0.044583111936912445 1109 72-73 -0.34502283805833045 1109 74-75 1.3849704390789377 1109 76 -0.4834806076258573 1110 1 0.7853872594862565 1110 2 0.7143746368537194 1110 3 0.11408952012933327 1110 4 0.7248378479813411 1110 5 0.6931642088236494 1110 6 0.6805625503229749 1110 7 1.2553558515594538 1110 8 2.0146269309918594 1110 9 -0.2646526425662543 1110 10-11 -0.37303683246439334 1110 12-13 1.1332747629400792 1110 14-15 1.1174928026439126 1110 16-17 0.8173692357457654 1110 18-19 0.8379317456793487 1110 20-21 0.368777014607403 1110 22-23 1.2760031812292745 1110 24-25 0.27811954443064124 1110 26-27 0.27367141659539485 1110 28-29 0.34196664893736184 1110 30-31 0.3882695575419426 1110 32-33 1.437855928603259 1110 34-35 1.0399525861438903 1110 36-37 0.7440243315289372 1110 38-39 1.017895993114962 1110 40-41 0.9127496267536905 1110 42-43 1.3389052671431472 1110 44-45 1.852362853020054 1110 46-47 1.0074665435317733 1110 48-49 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40-41 -1.324929228572227 2116 42-43 -1.3322791525706847 2116 44-45 -0.8014129816381406 2116 46-47 -0.8174937108402425 2116 48-49 -1.1562956032180516 2116 50-51 -0.41123422481780025 2116 52-53 -0.23029964628734945 2116 54-55 0.3880217771702128 2116 56-57 -0.04403537988856243 2116 58-59 -0.5866024541494497 2116 60-61 -0.8359136104631091 2116 62-63 0.04659470744349292 2116 64-65 -1.1142027344399672 2116 66-67 0.6686468283883933 2116 68-69 0.3566952431825001 2116 70-71 -2.5090992409691744 2116 72-73 -0.47898868246060644 2116 74-75 0.06599510701062528 2116 76 -0.9164976854247193 2117 1 2.7011530252520224 2117 2 1.6980683305474145 2117 3 1.6151706012104157 2117 4 1.683036046179538 2117 5 1.212984028643472 2117 6 0.619841829602251 2117 7 0.2776981939017986 2117 8 1.529852156217089 2117 9 2.5792212313076206 2117 10-11 1.784080284652724 2117 12-13 1.5457522404175528 2117 14-15 1.055285595436711 2117 16-17 1.9593962627727919 2117 18-19 1.6474362501838513 2117 20-21 1.9096328704632555 2117 22-23 1.4618139920400814 2117 24-25 1.677083508394606 2117 26-27 2.071419164343144 2117 28-29 1.6551648471355627 2117 30-31 1.0852965845689724 2117 32-33 1.7986216943690216 2117 34-35 1.3791868203781164 2117 36-37 1.5778982054028141 2117 38-39 2.174247344466316 2117 40-41 2.073109987114048 2117 42-43 0.9073286905665725 2117 44-45 2.2801556458128402 2117 46-47 1.5836827597479903 2117 48-49 1.2637043967819501 2117 50-51 2.0683736183194554 2117 52-53 1.6434258439087301 2117 54-55 1.4566492281506065 2117 56-57 0.8492979534447684 2117 58-59 1.3082995066348637 2117 60-61 2.4491844287525772 2117 62-63 1.8975750996003562 2117 64-65 1.2077580498737568 2117 66-67 1.3145291813295685 2117 68-69 1.866107007888381 2117 70-71 2.4909007590308256 2117 72-73 2.1366975920491917 2117 74-75 2.25658334230474 2117 76 1.2223258439870435 2118 1 0.019101743200739207 2118 2 0.5555042279833131 2118 3 1.1182475242873373 2118 4 -0.7564511333076425 2118 5 0.4232404388998816 2118 6 0.4983033680637874 2118 7 0.8541084503120544 2118 8 -0.933224766859837 2118 9 0.8720417441281327 2118 10-11 1.2504905410629803 2118 12-13 1.0947265993919082 2118 14-15 1.2019522621033722 2118 16-17 0.4758065191830525 2118 18-19 0.4161541989018005 2118 20-21 -0.1888286679982798 2118 22-23 0.41694219716828584 2118 24-25 0.4824681237792241 2118 26-27 2.34834224126622 2118 28-29 1.2549084368791448 2118 30-31 0.9858094050817883 2118 32-33 2.155544771292103 2118 34-35 1.7132893844806887 2118 36-37 1.0089238464284556 2118 38-39 0.6416832419022143 2118 40-41 -0.32945411545005143 2118 42-43 0.5668158700537518 2118 44-45 0.5457966714538642 2118 46-47 1.0185545546197865 2118 48-49 0.4911402942178462 2118 50-51 0.36093772088355536 2118 52-53 -0.12170236121947653 2118 54-55 1.264341535842913 2118 56-57 1.6303235944704149 2118 58-59 1.6388123271476864 2118 60-61 0.515594685162835 2118 62-63 1.2603956124208686 2118 64-65 1.2454503575660674 2118 66-67 -1.2549579981576109 2118 68-69 1.525850597631969 2118 70-71 0.7703879385180059 2118 72-73 0.29695400230560764 2118 74-75 1.5724807782021806 2118 76 2.3551463568075555 2119 1 1.6627349461559753 2119 2 1.1947914943892215 2119 3 0.3392383978205835 2119 4 0.928685763693661 2119 5 1.8321930681914935 2119 6 0.8383729030485796 2119 7 -1.8316803371716475 2119 8 1.1565188228837542 2119 9 0.9157184064488604 2119 10-11 0.8260011886075276 2119 12-13 0.09027201442885158 2119 14-15 0.76974887227286 2119 16-17 0.6854979576880496 2119 18-19 0.555006871652779 2119 20-21 0.3270340004067549 2119 22-23 1.0126614496672026 2119 24-25 -0.9478882430178253 2119 26-27 1.0623796163205448 2119 28-29 0.2513230392259551 2119 30-31 0.004279635416430949 2119 32-33 0.33003412374755925 2119 34-35 -0.2674798462885448 2119 36-37 -0.16458767030340482 2119 38-39 0.07051853090699467 2119 40-41 1.388929196153601 2119 42-43 -0.48272780660856895 2119 44-45 0.2546189226489979 2119 46-47 0.4524963190700255 2119 48-49 -0.08849899304856024 2119 50-51 1.4773001719917715 2119 52-53 0.7048947704624027 2119 54-55 -0.21566715603018594 2119 56-57 0.4610493658741497 2119 58-59 0.2575650433580279 2119 60-61 1.0148341462667005 2119 62-63 0.36763159677549595 2119 64-65 0.2583795188003073 2119 66-67 0.23385121522787244 2119 68-69 0.5420392112782118 2119 70-71 -0.2565568680878165 2119 72-73 -0.12036455484345865 2119 74-75 0.4586172406098292 2119 76 0.78300381008874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 13.0 13 117.0 14 410.0 15 887.0 16 1402.0 17 1850.0 18 1946.0 19 1789.0 20 1438.0 21 1096.0 22 761.0 23 569.0 24 496.0 25 462.0 26 485.0 27 514.0 28 575.0 29 672.0 30 865.0 31 1137.0 32 1532.0 33 2010.0 34 3026.0 35 4929.0 36 8810.0 37 11132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.671875 22.008634868421055 10.236430921052632 29.08305921052631 2 16.64111840088295 29.68299711815562 30.21031332393157 23.46557115702986 3 14.836388905921066 31.8147444151491 27.506284873382796 25.842581805547038 4 10.589245202035686 28.579312036298976 33.01244711508983 27.818995646575512 5 9.830972673574918 35.54887894209741 31.371226521143747 23.248921863183927 6 20.683875978498577 37.52733664438858 16.714697406340058 25.074089970772782 7 20.252621252069407 32.021174402681545 19.694647127352997 28.031557217896047 8 17.824514071984794 39.178776544648144 18.198540683058432 24.798168700308622 9 19.68238395977681 22.478386167146976 18.977251824146176 38.86197804893004 10-11 19.800927913013265 27.63402620230139 26.0806916426513 26.48435424203405 12-13 19.369673186584095 25.10270402845055 25.5901649396039 29.937457845361457 14-15 18.99769043677315 27.036196782962374 22.92088213052098 31.045230649743495 16-17 20.705336521756905 28.397408383918897 23.948944345657814 26.948310748666383 18-19 18.9987123674045 27.878267623193736 24.578453614568645 28.544566394833122 20-21 18.826006090706564 28.03768880168414 24.44560263249331 28.690702475115987 22-23 21.133525456292027 28.074478304412697 24.02763300427167 26.76436323502361 24-25 19.963414883397714 29.946655221043596 23.544259815643713 26.545670079914974 26-27 18.47854967604799 30.30126515012161 25.232489218631837 25.987695955198557 28-29 20.273059864696386 28.15316696302655 26.870644020683876 24.70312915159319 30-31 19.904142906779487 27.91710098718499 26.635599975473667 25.543156130561854 32-33 17.9420360945899 30.340098514112864 26.005068775931488 25.71279661536575 34-35 19.003822020561245 28.84501400044965 26.19208208146831 25.959081897520797 36-37 19.54442332454473 27.866004455617556 26.66932368630817 25.920248533529545 38-39 19.159155476526255 27.16291618124962 27.860894802460813 25.817033539763322 40-41 18.3886197804893 26.824657142273185 27.505262942751447 27.281460134486068 42-43 17.099965254358533 27.163938111880963 28.575224313773578 27.160872319986918 44-45 17.646698142130113 27.51139452653954 28.481206695689497 26.360700635640853 46-47 17.75604471968443 28.372882048766527 27.380587405726896 26.49048582582214 48-49 17.51895681321152 29.003413248308707 26.222740000408773 27.254889938071003 50-51 17.571075275410305 28.28295215320784 26.323911132912297 27.822061438469557 52-53 17.188873219285874 27.777096490690212 26.928894066670754 28.10513622335316 54-55 16.880250168618556 28.521061990312095 26.218652277883375 28.380035563185974 56-57 17.897071146810557 28.019294050319864 25.892656406483127 28.190978396386452 58-59 18.094303758660864 27.465407648128842 26.098064463384222 28.342224129826064 60-61 16.873096654199113 27.367302307519363 26.089889018333434 29.669712019948086 62-63 17.487276963639708 27.80979827089337 25.83440636049625 28.868518404970672 64-65 17.75400085842173 28.54252253357042 24.495677233429394 29.207799374578453 66-67 17.75502278905308 29.80562879391747 23.753755595070206 28.685592821959244 68-69 18.010505446890264 29.485764506305312 23.628058127414313 28.87567191939011 70-71 18.441760173319434 29.814826169599606 23.57083001205878 28.172583645022176 72-73 17.806119320620517 29.16283442679911 23.152860383837144 29.87818586874323 74-75 18.078974799190632 29.05553171050749 23.335785966848572 29.529707523453308 76 18.11883009381323 27.081161730741716 24.064422507000224 30.735585668444827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 1.5 14 4.0 15 5.5 16 6.0 17 7.0 18 9.5 19 12.5 20 19.5 21 26.0 22 32.5 23 51.0 24 85.5 25 108.0 26 136.5 27 181.0 28 252.0 29 307.0 30 367.0 31 461.0 32 618.0 33 741.0 34 831.5 35 1067.0 36 1350.5 37 1489.0 38 1645.0 39 1996.0 40 2463.5 41 2826.5 42 2917.0 43 3059.0 44 3311.5 45 3422.5 46 3423.0 47 3460.0 48 3381.5 49 3123.5 50 2981.0 51 2799.0 52 2382.0 53 1991.0 54 1835.0 55 1739.0 56 1450.0 57 1142.5 58 1028.0 59 907.5 60 665.0 61 457.0 62 371.0 63 338.0 64 252.0 65 174.5 66 121.0 67 92.0 68 77.0 69 55.0 70 38.5 71 29.0 72 23.5 73 18.5 74 16.5 75 14.0 76 12.0 77 9.0 78 8.5 79 9.0 80 10.0 81 8.5 82 4.5 83 3.0 84 4.0 85 4.0 86 3.0 87 3.0 88 4.0 89 3.5 90 3.5 91 4.5 92 4.0 93 2.5 94 2.5 95 4.0 96 4.0 97 2.5 98 1.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5865881823941791 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48927.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.92701371430907 #Duplication Level Percentage of deduplicated Percentage of total 1 82.7583307642723 60.35317922619413 2 9.369132031052942 13.665256402395407 3 3.6490008688097304 7.9833220920963885 4 1.661948936408733 4.848038915118442 5 0.8519940584624871 3.106669119300182 6 0.6922451725007707 3.029002391317677 7 0.3223003839578487 1.6453083164714781 8 0.20739329054678962 1.209965867516913 9 0.16815672206496454 1.1036850818566435 >10 0.31949777192343265 3.0555725877327444 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004087722525394976 0.0 2 0.0 0.0 0.0 0.004087722525394976 0.0 3 0.0 0.0 0.0 0.004087722525394976 0.0 4 0.0 0.0 0.0 0.004087722525394976 0.0 5 0.0 0.0 0.0 0.004087722525394976 0.0 6 0.0 0.0 0.0 0.006131583788092464 0.0 7 0.0 0.0 0.0 0.006131583788092464 0.0 8 0.0 0.0 0.0 0.008175445050789952 0.0 9 0.0 0.0 0.0 0.008175445050789952 0.0 10 0.0 0.0 0.0 0.008175445050789952 0.0 11 0.0 0.0 0.0 0.01021930631348744 0.0 12 0.0 0.0 0.0 0.01021930631348744 0.0 13 0.0 0.0 0.0 0.01021930631348744 0.0 14 0.0 0.0 0.0 0.01021930631348744 0.0 15 0.0 0.0 0.0 0.012263167576184928 0.0 16 0.0 0.0 0.0 0.014307028838882416 0.0 17 0.0 0.0 0.0 0.014307028838882416 0.0 18 0.0 0.0 0.0 0.016350890101579904 0.0 19 0.0 0.0 0.0 0.024526335152369856 0.0 20 0.0 0.0 0.0 0.030657918940462322 0.0 21 0.0 0.0 0.0 0.030657918940462322 0.0 22 0.0 0.0 0.0 0.0347456414658573 0.0 23 0.0 0.0 0.0 0.03678950272855479 0.0 24 0.0 0.0 0.0 0.03678950272855479 0.0 25 0.0 0.0 0.0 0.03883336399125227 0.0 26 0.0 0.0 0.0 0.04087722525394976 0.0 27 0.0 0.0 0.0 0.04496494777934474 0.0 28 0.0 0.0 0.0 0.059271976618227154 0.0 29 0.0 0.0 0.0 0.06540356040631962 0.0 30 0.0 0.0 0.0 0.0858421730332945 0.0 31 0.0 0.0 0.0 0.12671939828724427 0.0 32 0.0 0.0 0.0 0.14511414965152167 0.0 33 0.0 0.0 0.0 0.16759662354119403 0.0 34 0.0 0.0 0.0 0.20029840374435384 0.0 35 0.0 0.0 0.0 0.26161424162527847 0.0 36 0.0 0.0 0.0 0.3638073047601529 0.0 37 0.0 0.0 0.0 0.5048337318862796 0.0 38 0.0 0.0 0.0 0.7112637194187259 0.0 39 0.0 0.0 0.0 0.9728779610440044 0.0 40 0.0 0.0 0.0 1.2385799251946779 0.0 41 0.0 0.0 0.0 1.5860363398532507 0.0 42 0.0 0.0 0.0 1.9948085923927483 0.0 43 0.0 0.0 0.0 2.3361334232632287 0.0 44 0.0 0.0 0.0 2.710160034336869 0.0 45 0.0 0.0 0.0 3.0310462525803747 0.0 46 0.0 0.0 0.0 3.44186236638257 0.0 47 0.0 0.0 0.0 3.86494164776095 0.0 48 0.0 0.0 0.0 4.2307928137838005 0.0 49 0.0 0.0 0.0 4.631389621272508 0.0 50 0.0 0.0 0.0 4.9992846485580555 0.0 51 0.0 0.0 0.0 5.3835305659451835 0.0 52 0.0 0.0 0.0 5.859750240153699 0.0 53 0.0 0.0 0.0 6.2542154638543135 0.0 54 0.0 0.0 0.0 6.656856132605719 0.0 55 0.0 0.0 0.0 6.9920493796881065 0.0 56 0.0 0.0 0.0 7.38038301960063 0.0 57 0.0 0.0 0.0 7.825944774868682 0.0 58 0.0 0.0 0.0 8.17748891205265 0.0 59 0.0 0.0 0.0 8.56786641322787 0.0 60 0.0 0.0 0.0 8.950068469352301 0.0 61 0.0 0.0 0.0 9.326138941688638 0.0 62 0.0 0.0 0.0 9.818709505998733 0.0 63 0.0 0.0 0.0 10.254051954953297 0.0 64 0.0 0.0 0.0 10.652604901179307 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACAT 15 0.00221305 69.99284 32 CGATGCC 15 0.00221305 69.99284 16 AACCGTA 20 0.0069152927 52.494633 58 GGTCGTG 20 0.0069152927 52.494633 53 TCGTGGT 20 0.0069152927 52.494633 55 GTCGTGG 20 0.0069152927 52.494633 54 CGAATGT 20 0.0069152927 52.494633 29 CTTTACC 20 0.0069152927 52.494633 59 GACTAGG 20 0.0069152927 52.494633 2 GAAACCC 40 4.3443473E-5 44.060894 1 CGACGAA 40 0.0023981305 34.99642 22 AGCAATA 50 0.007147676 27.997137 49 AAACCCA 135 5.8379774E-6 20.73862 2 AACCCAC 100 0.009567856 17.49821 3 ATACACA 530 0.0011754434 7.263408 4 >>END_MODULE