FastQCFastQC Report
Wed 25 May 2016
SRR1780390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48927
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT33516.848979091299283RNA PCR Primer, Index 35 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT14833.0310462525803747No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC10162.076563042900648No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC4420.9033866781122897No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT2510.5130091769370695No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1160.23708790647290862No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1150.23504404521021116No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1110.2268686001594212No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1080.2207370163713287No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT1020.2084738487951438No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT1010.2064299875324463No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT930.1900790974308664No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC750.1532895947023116No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC750.1532895947023116No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT720.14715801091421915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC580.11854395323645431No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT550.11241236944836185No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC550.11241236944836185No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC550.11241236944836185No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT510.1042369243975719No Hit
ATACACATCTCCGAGCCCACGAGACTGGGCTTCATCTCGTATGCCGTCTT500.1021930631348744TruSeq Adapter, Index 11 (95% over 23bp)
CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG500.1021930631348744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAT150.002212152470.0000115
TTTATAC301.00391844E-770.000013
CTTTATA301.00391844E-770.000012
CAACTAG150.002212152470.0000143
CTCTCGT150.002212152470.0000134
CCTGCTG150.002212152470.0000134
CCTCTCG150.002212152470.0000133
TTTACAC1300.064.615392
CTATACA800.056.8751
CTTATAA452.6326234E-854.4444431
CTTTACA1550.054.1935461
CACCTCG407.3366937E-752.536
ATCCCGT200.00691249852.537
TTGAAAC200.00691249852.555
TCCCGTA200.00691249852.538
TTTGCCG200.00691249852.555
CGAGAAG200.00691249852.54
TTACACA1700.051.4705853
TATAACC305.82405E-446.6666683
CCTCGTA451.6526719E-646.66666438
Produced by FastQC (version 0.11.5)