FastQCFastQC Report
Wed 25 May 2016
SRR1780389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565597
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGG300.0057028829.37486817
CAGGTAC407.0311833E-427.497511
CGGTGGT1750.026.37660443
CGTGCGA959.586074E-1025.47814410
CTTCCGA450.001383675124.48556518
CCAACGT651.814928E-523.68597222
CATATTC752.078581E-623.4437983
CCGAACG1004.570029E-822.01944213
GTACAAG904.0774103E-721.998011
CTTATAA500.002580108821.9980091
CATCGTT602.8856925E-421.98244932
TCGACCT602.8891463E-421.9785615
TCGGTGG2900.021.22443242
TGCGTGT1900.020.82547833
AAAAGAC1502.1827873E-1120.5314751
TCGTTGT654.9189426E-420.31304634
GCGTGTA1750.020.11958734
CTATCGG550.004473879520.0052738
TACCGAA1101.2603232E-720.003511
TTAAGCA550.00450606519.9805094
Produced by FastQC (version 0.11.5)