FastQCFastQC Report
Wed 25 May 2016
SRR1780389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565597
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT351426.213257849670349No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT333085.888998704024243RNA PCR Primer, Index 45 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC92331.6324344011725662RNA PCR Primer, Index 45 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC49950.8831376404047405No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG41920.7411637614768112No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT30690.5426124961766063RNA PCR Primer, Index 45 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC19690.3481277305219087No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC16270.2876606488365391No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG13500.23868584875803797No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG12160.21499406821464756No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC11210.1981976566353782No Hit
CTTAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC7510.13278005364243445RNA PCR Primer, Index 45 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC7280.12871355399692713No Hit
CTTATTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG5710.10095527380802939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT33650.042.49722344
GCCGTCT37900.037.67365643
CTATACA7450.037.2352141
TTTACAC11100.036.47252
GATCTTT250.002343332935.2256971
CTTTACA11500.035.2256971
CTTATAA2100.032.4998971
TTACACA12500.032.3875773
CTTATAC53750.030.064721
TTATACA53600.029.924952
CGTACAC300.0057393629.3365763
TCGTTCC300.005744322729.3313924
CGTTCCA300.005744322729.3313925
AGAGTCT300.005744322729.331396
CGTGCGA554.9506652E-628.00309410
CTTTATA1900.027.7925452
TCAAGAG407.022934E-427.503043
TTATACG407.030198E-427.49817727
TACACAG407.030198E-427.4981775
TTTTACG407.030198E-427.49817729
Produced by FastQC (version 0.11.5)