FastQCFastQC Report
Wed 25 May 2016
SRR1780388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39969
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTGG150.002210272269.99123420
CCTGGTT150.002210272269.99123422
GTTAGGC200.006906682652.49342755
CTGGTTC200.006906682652.49342723
TGGTTCA200.006906682652.49342724
GAAAGAT200.006906682652.49342739
ATAAGCG200.006906682652.49342770
CATACTT200.006906682652.49342722
GCAGCCT200.006906682652.4934278
GATTCTC200.006906682652.49342734
TGCAGCA200.006906682652.49342767
TATAAGC200.006906682652.49342769
TAGCTGA200.006906682652.49342759
ACCACAC1508.203642E-1025.6634522
CACACCT952.7191773E-422.1024954
ACACCTC800.002624689821.8722635
AACACAC1205.5481883E-520.414112
GACGAGG1854.528285E-617.02489770
CGACGAG1957.4724358E-616.15182569
AACCACA1350.002718086615.6910781
Produced by FastQC (version 0.11.5)