##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780388_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39969 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.947134028872377 33.0 23.0 34.0 12.0 34.0 2 29.068052740874176 34.0 24.0 34.0 12.0 34.0 3 29.212014311091096 34.0 27.0 34.0 11.0 34.0 4 29.461207435762716 34.0 30.0 34.0 11.0 34.0 5 29.485050914458707 34.0 31.0 34.0 11.0 34.0 6 31.53248767795041 37.0 27.0 38.0 11.0 38.0 7 31.41319522630038 37.0 27.0 38.0 11.0 38.0 8 30.1308263904526 35.0 26.0 38.0 11.0 38.0 9 31.073131677049712 36.0 27.0 38.0 12.0 38.0 10-11 31.2872476169031 37.0 27.0 38.0 11.0 38.0 12-13 31.53097400485376 37.0 27.0 38.0 11.0 38.0 14-15 31.24873026595612 37.0 27.0 38.0 11.0 38.0 16-17 31.07134279066276 37.0 26.0 38.0 11.0 38.0 18-19 31.09923941054317 37.0 26.5 38.0 11.0 38.0 20-21 31.201643773924793 37.0 26.5 38.0 11.0 38.0 22-23 31.007668443043357 37.0 26.0 38.0 11.0 38.0 24-25 31.446846305887064 37.0 27.0 38.0 11.0 38.0 26-27 31.42043833971328 37.0 27.0 38.0 11.0 38.0 28-29 31.421601741349548 37.0 27.0 38.0 11.0 38.0 30-31 31.309414796467262 37.0 27.0 38.0 11.0 38.0 32-33 31.096099477094747 37.0 26.5 38.0 11.0 38.0 34-35 31.252983562260752 37.0 26.5 38.0 11.0 38.0 36-37 31.14877780279717 37.0 26.5 38.0 11.0 38.0 38-39 31.445132477670192 37.0 27.0 38.0 11.0 38.0 40-41 31.08399009232155 37.0 26.0 38.0 11.0 38.0 42-43 31.339950961995548 37.0 27.0 38.0 11.0 38.0 44-45 30.534739423052866 36.5 25.5 38.0 11.0 38.0 46-47 31.19802847206585 37.0 27.0 38.0 11.0 38.0 48-49 31.154544772198456 37.0 26.5 38.0 11.0 38.0 50-51 30.990267457279394 37.0 26.5 38.0 11.0 38.0 52-53 30.940691535940353 37.0 26.0 38.0 11.0 38.0 54-55 30.769158597913382 37.0 26.0 38.0 11.0 38.0 56-57 30.67225850033776 36.5 25.5 38.0 11.0 38.0 58-59 30.62101878956191 36.5 25.5 38.0 11.0 38.0 60-61 30.72289774575296 36.5 25.5 38.0 11.0 38.0 62-63 30.66868072756386 37.0 25.5 38.0 11.0 38.0 64-65 30.736032925517275 37.0 25.0 38.0 11.0 38.0 66-67 30.754634842002552 37.0 25.0 38.0 11.0 38.0 68-69 30.11093597538092 36.0 24.0 38.0 11.0 38.0 70-71 30.276151517426005 36.0 24.0 38.0 11.0 38.0 72-73 30.520691035552552 36.0 25.0 38.0 11.0 38.0 74-75 30.07649678500838 36.0 24.0 38.0 11.0 38.0 76 29.3296304636093 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.0453233603242715 1101 2 -0.05290739814188328 1101 3 0.4472665388670407 1101 4 -1.9825597372046673 1101 5 0.5046401722295997 1101 6 -1.6345243700208805 1101 7 -0.1429005868349833 1101 8 -0.9936175763927118 1101 9 0.41291185005031394 1101 10-11 -0.5425866823826944 1101 12-13 -0.6368659308358495 1101 14-15 -0.19854892395678192 1101 16-17 -1.9254408163183214 1101 18-19 0.45090313720914565 1101 20-21 -1.0245641158174728 1101 22-23 0.20828000264085134 1101 24-25 -0.9852954344700748 1101 26-27 -1.1281380643916386 1101 28-29 -0.8350165102194147 1101 30-31 -0.38017719760293645 1101 32-33 0.6759640027448626 1101 34-35 -0.20938503261817232 1101 36-37 0.6595143682671853 1101 38-39 0.3071463542954369 1101 40-41 0.2576169222929323 1101 42-43 0.4281570931314249 1101 44-45 0.36101675939245936 1101 46-47 0.5505389986878271 1101 48-49 -0.1730182232135249 1101 50-51 0.3759775287257092 1101 52-53 1.0536887548567648 1101 54-55 -0.6851994193924718 1101 56-57 0.28837311657810005 1101 58-59 1.4517217425290525 1101 60-61 1.194609724324316 1101 62-63 0.16515213430932718 1101 64-65 -0.32962406262026533 1101 66-67 0.47840122088184245 1101 68-69 1.428428269063481 1101 70-71 0.7534765954006808 1101 72-73 0.1567262088197765 1101 74-75 -0.05222060085192126 1101 76 -1.5289161130218218 1102 1 -0.0653123319261475 1102 2 -0.6522319087567148 1102 3 -0.2180905055222624 1102 4 -0.8148591582137676 1102 5 0.3982161303216891 1102 6 0.5277309373957202 1102 7 1.1269505297903244 1102 8 0.7383234216773182 1102 9 -0.018781009061900278 1102 10-11 -1.1143057339404514 1102 12-13 -3.6998311913817545 1102 14-15 -2.09974136950407 1102 16-17 -2.9223390793456154 1102 18-19 -1.489784759123907 1102 20-21 -1.3733716702701848 1102 22-23 -0.8585107335047262 1102 24-25 -0.3050500526117901 1102 26-27 -4.956853256010056 1102 28-29 -0.5838447429186147 1102 30-31 -1.2515585044681181 1102 32-33 -6.226193427638375 1102 34-35 -1.0104603014353764 1102 36-37 -0.2365429794030689 1102 38-39 -1.122306361447599 1102 40-41 -1.004548779388898 1102 42-43 -1.1367036733422466 1102 44-45 -4.7833174010707324 1102 46-47 -0.9909208855139937 1102 48-49 -1.206689301239443 1102 50-51 -6.1845063422420345 1102 52-53 -2.1601587775391593 1102 54-55 -0.9213794579918648 1102 56-57 -2.2988546198431834 1102 58-59 -1.15880348493166 1102 60-61 -0.8275504631584525 1102 62-63 -3.350459941786621 1102 64-65 -1.49039742903879 1102 66-67 -1.4512238135818976 1102 68-69 -5.918449315717329 1102 70-71 -1.5954343502844566 1102 72-73 -0.855887521535891 1102 74-75 -5.797257821695592 1102 76 -2.0105814011387224 1103 1 -0.49564387314478253 1103 2 -0.6159415861760706 1103 3 -1.1654471363466357 1103 4 -0.3303161474610796 1103 5 -0.4649111098216814 1103 6 -1.5340970195935313 1103 7 0.5787874473530508 1103 8 2.1527499449748113 1103 9 0.33919390133236504 1103 10-11 -0.5303003575963672 1103 12-13 -2.300861657331577 1103 14-15 -1.0519143085721687 1103 16-17 -1.4131544915319942 1103 18-19 -1.2806673756113653 1103 20-21 -0.9628653978687538 1103 22-23 0.09556632742717497 1103 24-25 -0.19069073361537647 1103 26-27 -0.9828389190924938 1103 28-29 -0.23191821962112158 1103 30-31 -1.3785746335003743 1103 32-33 -4.506995398964541 1103 34-35 -1.1052183659515045 1103 36-37 -0.6495134095105968 1103 38-39 -0.11726603886695486 1103 40-41 -0.6553104281344204 1103 42-43 -0.7753685478942174 1103 44-45 -4.711632813257111 1103 46-47 -0.8113733944745647 1103 48-49 -1.190112240307542 1103 50-51 -0.9573558046076229 1103 52-53 0.04727849844650578 1103 54-55 0.19353989684684691 1103 56-57 0.4163111507661341 1103 58-59 -0.6147846677273563 1103 60-61 0.042633228597821926 1103 62-63 -0.5781705152633201 1103 64-65 -0.3125300455262483 1103 66-67 -0.5512462150155919 1103 68-69 -3.0242960899108766 1103 70-71 -1.141021267847183 1103 72-73 -0.07911780827424053 1103 74-75 -2.929090258971577 1103 76 -1.3638520104577196 1104 1 -1.480605903520484 1104 2 -1.3488552552408173 1104 3 -1.3966122042922784 1104 4 -0.4815451642476489 1104 5 -1.1123431641781956 1104 6 -1.8620346694736263 1104 7 0.16866554487503294 1104 8 0.31751813042644983 1104 9 -1.3791314383958557 1104 10-11 -1.6650425638313813 1104 12-13 -1.6623224886665113 1104 14-15 -1.3161778977008645 1104 16-17 -0.8972172413321537 1104 18-19 -0.46759283524365713 1104 20-21 -0.4536977320014479 1104 22-23 -0.5524652473130338 1104 24-25 -0.9563032436073797 1104 26-27 -1.000424850347489 1104 28-29 -1.207887444241429 1104 30-31 -1.4563624032845475 1104 32-33 -2.09904675787741 1104 34-35 -1.0319269990450302 1104 36-37 -1.5115487812132322 1104 38-39 -1.8486059669245094 1104 40-41 -0.26827006042858415 1104 42-43 -0.9135080363034902 1104 44-45 -3.947044483920582 1104 46-47 -1.6032149212531408 1104 48-49 -1.146147412170052 1104 50-51 -0.40819513314479394 1104 52-53 -0.9872938436797938 1104 54-55 -2.073996074376172 1104 56-57 -0.3564956038301865 1104 58-59 -0.49330684998155405 1104 60-61 -1.261148745822645 1104 62-63 -0.6011271499555697 1104 64-65 0.1163268689421777 1104 66-67 -0.3895505715303784 1104 68-69 -3.031265514371306 1104 70-71 -1.602976703498662 1104 72-73 -1.353972924181516 1104 74-75 -2.2603002909459953 1104 76 -1.4055686371563603 1105 1 -0.44255848910302475 1105 2 -0.8785254787935202 1105 3 -0.6679173078604315 1105 4 -0.6925924338145464 1105 5 -0.673011408106543 1105 6 0.469817969220788 1105 7 1.2499749939600626 1105 8 0.5560870068689177 1105 9 1.5260880102063368 1105 10-11 0.9276135126497174 1105 12-13 -0.9837941703815396 1105 14-15 1.013405415514331 1105 16-17 0.7800315636507058 1105 18-19 0.5749208048509757 1105 20-21 0.1426761755838939 1105 22-23 0.5289649105740821 1105 24-25 0.46313570188052466 1105 26-27 0.19799627852122725 1105 28-29 0.5611000503266794 1105 30-31 0.3684891248888853 1105 32-33 0.5587437882092132 1105 34-35 0.5210400233102384 1105 36-37 -0.09029920369403754 1105 38-39 0.20257456515989247 1105 40-41 0.52389352414 1105 42-43 0.7561843976687967 1105 44-45 0.7026102889054542 1105 46-47 1.0414183654061233 1105 48-49 0.22860513478567412 1105 50-51 0.6813466562424892 1105 52-53 -0.450670793126946 1105 54-55 -0.6776849353679175 1105 56-57 0.04329194867813868 1105 58-59 0.6846420660534029 1105 60-61 0.8629399027216067 1105 62-63 0.171433325154279 1105 64-65 -0.06830931472833512 1105 66-67 -0.4437005401460894 1105 68-69 0.814420353042145 1105 70-71 0.9644270915860744 1105 72-73 0.8215859575721396 1105 74-75 -0.6301762022974522 1105 76 -0.828290862061003 1106 1 -0.12169942870033879 1106 2 -0.33694158617607073 1106 3 -0.08305824745774615 1106 4 0.137850519205589 1106 5 -0.2822999987105703 1106 6 -0.4734303529268651 1106 7 0.3961763362419397 1106 8 0.028194389419255117 1106 9 0.24463834577680643 1106 10-11 0.10753297573696585 1106 12-13 0.2597494537795342 1106 14-15 0.8026134692056139 1106 16-17 0.5917899529124462 1106 18-19 0.011860402166412598 1106 20-21 -0.6137265089798625 1106 22-23 -0.3091558947950439 1106 24-25 0.7053370441624054 1106 26-27 0.01004996979639472 1106 28-29 -0.2719737751766793 1106 30-31 -0.3560746335003735 1106 32-33 0.644032378813236 1106 34-35 0.7652816340484954 1106 36-37 0.603069923822737 1106 38-39 -0.09001603886695264 1106 40-41 -0.0670326503566443 1106 42-43 0.42090922988356283 1106 44-45 0.616811631187332 1106 46-47 -0.05817895003012197 1106 48-49 -0.3123344625297655 1106 50-51 1.1479775287257148 1106 52-53 -0.1436103904423831 1106 54-55 1.1750398968468438 1106 56-57 0.6803389285439145 1106 58-59 0.44413199893930866 1106 60-61 1.0117721174867071 1106 62-63 0.43602392918112187 1106 64-65 1.08124773225153 1106 66-67 1.3816148960955132 1106 68-69 1.2774539100891218 1106 70-71 -0.014660156736070462 1106 72-73 0.04185441394798062 1106 74-75 -1.165451370082689 1106 76 -0.6841297882354986 1107 1 -1.2554904734764598 1107 2 -0.3603445712506961 1107 3 -0.5927895907413294 1107 4 0.5126564893548426 1107 5 0.2441477624834576 1107 6 1.1139129306552285 1107 7 0.9368927541523853 1107 8 1.3453287177774627 1107 9 -0.41225717661125216 1107 10-11 -0.19416851680034952 1107 12-13 0.3074509463168482 1107 14-15 -0.3277745904958813 1107 16-17 0.4781183111214027 1107 18-19 1.2457111484350634 1107 20-21 0.11585558057237577 1107 22-23 -0.7861708201681807 1107 24-25 -0.0924838513599866 1107 26-27 0.5329156414381906 1107 28-29 -0.9917648199527989 1107 30-31 -0.24517911111231427 1107 32-33 0.6565099907535341 1107 34-35 1.005789096735061 1107 36-37 0.4576072372555764 1107 38-39 0.2983123193419992 1107 40-41 0.23347481232992706 1107 42-43 0.08729728958505234 1107 44-45 0.40818476551569205 1107 46-47 0.5160001544474895 1107 48-49 0.30408344791799635 1107 50-51 0.7453805138003382 1107 52-53 1.1658522961247826 1107 54-55 0.6683533296826667 1107 56-57 1.283174749439432 1107 58-59 1.465594685506474 1107 60-61 -0.027899524304338286 1107 62-63 0.609277660524409 1107 64-65 0.12569549344555853 1107 66-67 0.8700328065432785 1107 68-69 1.0433345071040492 1107 70-71 0.37074282833855676 1107 72-73 0.5782723243957406 1107 74-75 -0.918466295455822 1107 76 0.20631797295853005 1108 1 -0.739598709275878 1108 2 -0.7877041761041284 1108 3 -0.6915762330692559 1108 4 -1.3448545167656363 1108 5 -0.4216956821638078 1108 6 1.116382596713425 1108 7 0.0895288542275523 1108 8 -0.725647337199451 1108 9 -0.9690594959498107 1108 10-11 -0.14345983001843265 1108 12-13 0.0894760724845689 1108 14-15 0.26655591524877664 1108 16-17 -0.6310301909724423 1108 18-19 -0.18342017337315397 1108 20-21 -0.3637696744474894 1108 22-23 -1.3834004991115911 1108 24-25 -1.3951521644706943 1108 26-27 -0.9932306057431752 1108 28-29 -1.2402615449608518 1108 30-31 -0.4599595255867044 1108 32-33 0.19232014859741042 1108 34-35 -0.2225744810594179 1108 36-37 -0.025937270421866998 1108 38-39 -0.14716711800364735 1108 40-41 -0.4984858877667122 1108 42-43 0.6692257766461509 1108 44-45 0.29755983262617747 1108 46-47 0.1989433521281505 1108 48-49 -0.19650712440026652 1108 50-51 -0.7643102410584639 1108 52-53 -0.4009629084279922 1108 54-55 -0.17471549883660842 1108 56-57 -0.13347402109637585 1108 58-59 -1.194745698902416 1108 60-61 -0.6532350767578947 1108 62-63 -1.259400531250531 1108 64-65 -0.11388895839595037 1108 66-67 -0.25285992404836577 1108 68-69 0.11621650001718109 1108 70-71 -0.09937958119650148 1108 72-73 0.14242995351632715 1108 74-75 0.26128244286695335 1108 76 -0.31923770190456224 1109 1 -1.0557284142075858 1109 2 -0.7409415861760706 1109 3 -0.4912321605012231 1109 4 0.00573457717660375 1109 5 -0.7677492740728908 1109 6 0.41155515431951173 1109 7 -0.3937367072363216 1109 8 0.4142233749265003 1109 9 0.6767542878057924 1109 10-11 0.6408952945775432 1109 12-13 -0.11819257520597404 1109 14-15 0.5424395561621331 1109 16-17 0.3582827065356362 1109 18-19 0.5673096775287334 1109 20-21 0.3866213171070889 1109 22-23 1.4666412066542343 1109 24-25 0.4063805224232695 1109 26-27 0.8009775060282784 1109 28-29 0.019591442214625943 1109 30-31 0.2563891346155671 1109 32-33 0.312901944030628 1109 34-35 -0.7456169166761413 1109 36-37 -0.12913297472798746 1109 38-39 0.4502158451910141 1109 40-41 0.34438763949842865 1109 42-43 0.9199816936516783 1109 44-45 0.022666703651097464 1109 46-47 -1.0286137326388172 1109 48-49 -0.23117504223990792 1109 50-51 0.7274268040880294 1109 52-53 0.6620127979634134 1109 54-55 0.2216196069917764 1109 56-57 -0.7185016511662354 1109 58-59 0.3095812743016282 1109 60-61 0.27255472618236 1109 62-63 -0.8443238969058342 1109 64-65 0.20437816703413958 1109 66-67 0.5304264902984173 1109 68-69 1.3593089825528928 1109 70-71 0.9361224519595766 1109 72-73 -0.20336297735636677 1109 74-75 0.4973892096274568 1109 76 -0.5374631215688304 1110 1 0.22528362214711706 1110 2 0.16583807484087743 1110 3 0.28806039661004945 1110 4 0.028087807341179882 1110 5 0.6726830521368861 1110 6 0.33172218944601894 1110 7 1.3225492175978708 1110 8 1.6416859148429843 1110 9 -0.9727175864265831 1110 10-11 -0.8759924479918482 1110 12-13 0.5231731825930908 1110 14-15 0.9446134692056098 1110 16-17 0.25904419020058356 1110 18-19 1.2836570123359081 1110 20-21 0.006002304579457274 1110 22-23 0.4972847831710574 1110 24-25 0.1670658577217239 1110 26-27 -0.9371703691866564 1110 28-29 0.014433004484338596 1110 30-31 0.022908417347082377 1110 32-33 0.4969476330505245 1110 34-35 0.4021968882857827 1110 36-37 -0.10696397448234407 1110 38-39 0.4055093848618583 1110 40-41 0.10022158693149308 1110 42-43 0.9377905858157618 1110 44-45 0.6311167159330964 1110 46-47 0.029787151664791622 1110 48-49 0.24020791035159306 1110 50-51 -0.15368348822344302 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0.7706904190661206 2116 46-47 0.7987907469395736 2116 48-49 0.14827159807629897 2116 50-51 1.0350684378166157 2116 52-53 0.6205108216788275 2116 54-55 -1.459869194062243 2116 56-57 -0.036024707819724 2116 58-59 -1.700958910151602 2116 60-61 -1.3591975794829878 2116 62-63 1.0812360503932403 2116 64-65 -0.8377825707787743 2116 66-67 -0.24032449784387566 2116 68-69 0.5694539100891234 2116 70-71 -2.6122965203724355 2116 72-73 -0.28208497999141 2116 74-75 -1.2684210670523832 2116 76 -0.4768570609627716 2117 1 0.36322364822273556 2117 2 0.34367379843931545 2117 3 1.399403291003793 2117 4 0.2738505192055882 2117 5 0.9798538474432767 2117 6 0.10564657015006063 2117 7 -0.43520827914267457 2117 8 0.6952713124961782 2117 9 1.8624844996229584 2117 10-11 0.8309175911215796 2117 12-13 1.6477494537795394 2117 14-15 0.24845962305176528 2117 16-17 1.6976361067586048 2117 18-19 0.6620142483202613 2117 20-21 0.3899657987124421 2117 22-23 1.2493056436664922 2117 24-25 1.770260121085478 2117 26-27 1.9291268928733238 2117 28-29 1.2923339171310175 2117 30-31 2.260848443422706 2117 32-33 1.821263148044011 2117 34-35 1.4418970186638767 2117 36-37 0.23814684689966015 2117 38-39 1.6370608842099692 2117 40-41 1.1311211957972063 2117 42-43 0.5726015375758706 2117 44-45 1.740503938879641 2117 46-47 1.9479748961237249 2117 48-49 1.610742460547165 2117 50-51 1.760592913341096 2117 52-53 1.546851148019158 2117 54-55 1.3156552814622309 2117 56-57 2.2849543131592966 2117 58-59 2.1004396912470042 2117 60-61 2.0723875021020888 2117 62-63 1.6600239291811256 2117 64-65 0.9114015784053784 2117 66-67 0.1997687422493648 2117 68-69 1.6463769870122036 2117 70-71 2.447493689417776 2117 72-73 2.661854413947978 2117 74-75 1.617010168378851 2117 76 1.1497163656106544 2118 1 1.8354531136725427 2118 2 2.4539736680612165 2118 3 0.8304332779659838 2118 4 0.8840200107310103 2118 5 0.8760728826453601 2118 6 2.0944340538527975 2118 7 1.4750915904792272 2118 8 -3.892212390241763 2118 9 0.5527061423869775 2118 10-11 0.5392617892962868 2118 12-13 1.586732504626994 2118 14-15 1.126816859036115 2118 16-17 1.140400122403971 2118 18-19 1.8090807411494652 2118 20-21 0.056849762206574894 2118 22-23 0.37864071537444843 2118 24-25 1.1967268746708726 2118 26-27 1.9992703087794403 2118 28-29 1.2220601231284007 2118 30-31 0.7601965529403039 2118 32-33 1.3147442432200194 2118 34-35 3.236942650997644 2118 36-37 0.9354089068735902 2118 38-39 1.6766958255398272 2118 40-41 0.24428938354166263 2118 42-43 1.6411804163242394 2118 44-45 0.7243370549161483 2118 46-47 1.169617660139373 2118 48-49 0.3080045205210844 2118 50-51 0.6047910880477438 2118 52-53 0.5185591010830422 2118 54-55 0.7666331171858296 2118 56-57 1.6257626573574697 2118 58-59 1.30135233792236 2118 60-61 -0.27663466217430965 2118 62-63 1.6888374885031574 2118 64-65 1.5584680712345822 2118 66-67 -2.075198663226516 2118 68-69 0.5101318761908189 2118 70-71 -0.41391439402420716 2118 72-73 -0.4771286368994758 2118 74-75 0.23787066381561672 2118 76 -0.7126043645066851 2119 1 1.5291577141568027 2119 2 1.020963175728692 2119 3 -0.7438201522196515 2119 4 -0.011863766508696472 2119 5 0.09560476319419209 2119 6 1.9778077423112315 2119 7 -1.7674427113771074 2119 8 -0.28971037248550857 2119 9 -0.25802832088985994 2119 10-11 1.817913928117914 2119 12-13 0.4977494537795337 2119 14-15 -0.07919605460391566 2119 16-17 0.21283757196006903 2119 18-19 0.5730032593092744 2119 20-21 1.3917973005439457 2119 22-23 1.9656060099668586 2119 24-25 0.8028608536862123 2119 26-27 1.0489071126535343 2119 28-29 1.340502415299511 2119 30-31 1.3386872712615308 2119 32-33 1.730603807384668 2119 34-35 2.1001387769056343 2119 36-37 0.13173659048940323 2119 38-39 1.0925553897044757 2119 40-41 1.673062587738599 2119 42-43 1.457766372740707 2119 44-45 1.3875735359492367 2119 46-47 0.042297240446067974 2119 48-49 0.5281417279464264 2119 50-51 1.6259775287257092 2119 52-53 0.2536277047957114 2119 54-55 0.285801801608752 2119 56-57 1.5289103571153433 2119 58-59 2.4938462846536 2119 60-61 2.1830102127248097 2119 62-63 0.4005001196573126 2119 64-65 2.09620011320391 2119 66-67 1.1298053722859933 2119 68-69 0.15278724342245908 2119 70-71 1.0132446051686905 2119 72-73 0.8142353663289335 2119 74-75 1.3809295822982683 2119 76 0.22444164033592884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 5.0 13 53.0 14 244.0 15 658.0 16 1097.0 17 1441.0 18 1578.0 19 1529.0 20 1255.0 21 869.0 22 666.0 23 504.0 24 421.0 25 421.0 26 415.0 27 488.0 28 586.0 29 692.0 30 889.0 31 1061.0 32 1487.0 33 1920.0 34 2884.0 35 4217.0 36 7242.0 37 7346.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.548891293951826 21.995419194080192 10.065188391935768 35.39050112003221 2 17.20833646075709 29.632965548299932 29.36776001401086 23.79093797693212 3 15.269333733643572 25.096950136355677 26.89584427931647 32.73787185068428 4 11.388826340413821 19.782831694563285 31.832169931697063 36.99617203332583 5 10.675773724636594 39.242913257774774 31.044059145838027 19.037253871750607 6 21.26397958417774 34.57679701768871 16.820535915334382 27.338687482799166 7 21.17641171908229 35.90032275013135 19.377517576121495 23.545747954664865 8 19.0272461157397 33.60104080662513 17.93389877154795 29.43781430608722 9 19.637719232405114 22.374840501388576 18.93217243363607 39.05526783257024 10-11 20.251945257574622 27.068478070504643 25.60109084540519 27.078485826515546 12-13 19.932948034726913 24.740423828467062 25.73744652105382 29.589181615752207 14-15 19.5626610623233 26.670669769071033 23.01658785558808 30.750081313017592 16-17 20.98626435487503 28.315694663364106 24.106182291275736 26.591858690485125 18-19 19.501363556756488 27.12977557607146 24.60907203082389 28.75978883634817 20-21 18.792063849483352 27.620155620605967 25.13197728239385 28.455803247516826 22-23 21.654282068603166 27.71397833320824 24.382646551077084 26.24909304711151 24-25 19.75280842653056 29.844379394030373 24.19124821736846 26.211563962070606 26-27 18.516850559183368 29.84938327203583 25.717431009031998 25.916335159748805 28-29 20.043033350846905 27.966674172483675 27.77527583877505 24.21501663789437 30-31 20.148114788961447 27.58888138307188 27.338687482799166 24.924316345167504 32-33 17.897620656008407 30.53616552828442 26.36168030223423 25.204533513472942 34-35 19.271185168505593 28.833596036928622 26.36543321073832 25.52978558382747 36-37 19.8716505291601 27.826565588330954 26.7094498236133 25.592334058895645 38-39 19.312467162050588 26.570592208961948 28.27065976131502 25.846280867672444 40-41 18.765793489954717 26.842052590757838 27.518827090995522 26.873326828291926 42-43 17.68370487127524 26.391703570266955 28.61592734369136 27.30866421476644 44-45 17.46853811704071 27.131026545572816 29.261427606394957 26.13900773099152 46-47 17.59613700617979 27.283644824739174 28.554629838124544 26.56558833095649 48-49 17.214591308263905 28.752283019339984 27.384973354349622 26.648152318046485 50-51 17.165803497710726 28.135555055167753 26.78450799369511 27.914133453426405 52-53 16.729215141734844 27.58387750506643 27.611398834096423 28.075508519102303 54-55 16.757987440266206 28.176837048712756 26.641897470539668 28.423278040481375 56-57 17.742500437839325 27.697715729690508 26.401711326277866 28.1580725061923 58-59 17.46353423903525 26.927118516850555 26.869573919787836 28.73977332432635 60-61 16.88933923790938 26.664414921564212 26.644399409542395 29.801846430984014 62-63 17.69121068828342 27.357452025319624 26.088968950936973 28.86236833545998 64-65 18.022717606144763 27.626410468112788 24.846756236082964 29.50411568965949 66-67 17.657434511746604 28.80232179939453 23.788436037929394 29.751807650929468 68-69 18.156571342790663 28.296930120843655 24.178738522354827 29.36776001401086 70-71 18.53936801020791 28.963696865070432 23.739648227376218 28.75728689734544 72-73 18.132802922264755 28.56713953313818 22.793915284345367 30.506142260251696 74-75 18.4230278465811 28.42202707098001 23.23050364032125 29.92444144211764 76 18.226625634867023 26.62563486702194 24.151217193324825 30.99652230478621 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 1.0 14 1.0 15 3.0 16 5.0 17 4.0 18 6.5 19 12.5 20 17.5 21 19.0 22 26.5 23 39.0 24 49.5 25 55.0 26 86.5 27 147.5 28 221.5 29 266.0 30 288.0 31 361.0 32 498.5 33 585.0 34 651.0 35 843.5 36 1102.5 37 1235.0 38 1392.0 39 1683.0 40 1971.5 41 2240.0 42 2354.0 43 2341.5 44 2504.0 45 2730.5 46 2782.0 47 2814.0 48 2671.0 49 2441.0 50 2386.0 51 2257.5 52 1956.5 53 1733.0 54 1682.0 55 1543.5 56 1235.0 57 980.5 58 896.0 59 771.5 60 592.0 61 464.0 62 391.0 63 336.0 64 240.0 65 182.0 66 133.0 67 101.0 68 93.5 69 71.5 70 48.5 71 40.0 72 38.5 73 29.5 74 17.5 75 13.0 76 12.0 77 11.5 78 13.0 79 14.0 80 10.0 81 4.5 82 4.0 83 5.0 84 6.5 85 5.0 86 3.0 87 4.0 88 3.5 89 3.0 90 1.5 91 1.0 92 2.0 93 1.5 94 1.0 95 2.0 96 3.0 97 2.0 98 0.5 99 4.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.595461482649053 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 39969.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.49410793364858 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6859597771693 68.11528934924567 2 8.787335158782614 13.970827391228202 3 2.842035690680767 6.77775275838775 4 1.3218770654329148 4.203257524581551 5 0.5854027004060052 2.3268032725362158 6 0.3115853082806156 1.4861517676199054 7 0.17939760173732414 0.9982736620881182 8 0.10386176942687188 0.660511896719958 9 0.09441979038806533 0.6755235307363207 >10 0.08812513769552764 0.7856088468563137 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005003878005454227 0.0 2 0.0 0.0 0.0 0.005003878005454227 0.0 3 0.0 0.0 0.0 0.005003878005454227 0.0 4 0.0 0.0 0.0 0.007505817008181341 0.0 5 0.0 0.0 0.0 0.007505817008181341 0.0 6 0.0 0.0 0.0 0.007505817008181341 0.0 7 0.0 0.0 0.0 0.007505817008181341 0.0 8 0.0 0.0 0.0 0.007505817008181341 0.0 9 0.0 0.0 0.0 0.010007756010908454 0.0 10 0.0 0.0 0.0 0.010007756010908454 0.0 11 0.0 0.0 0.0 0.010007756010908454 0.0 12 0.0 0.0 0.0 0.010007756010908454 0.0 13 0.0 0.0 0.0 0.010007756010908454 0.0 14 0.0 0.0 0.0 0.010007756010908454 0.0 15 0.0 0.0 0.0 0.010007756010908454 0.0 16 0.0 0.0 0.0 0.010007756010908454 0.0 17 0.0 0.0 0.0 0.010007756010908454 0.0 18 0.0 0.0 0.0 0.012509695013635568 0.0 19 0.0 0.0 0.0 0.015011634016362681 0.0 20 0.0 0.0 0.0 0.015011634016362681 0.0 21 0.0 0.0 0.0 0.02001551202181691 0.0 22 0.0 0.0 0.0 0.02251745102454402 0.0 23 0.0 0.0 0.0 0.02251745102454402 0.0 24 0.0 0.0 0.0 0.025019390027271136 0.0 25 0.0 0.0 0.0 0.027521329029998248 0.0 26 0.0 0.0 0.0 0.04253296304636093 0.0 27 0.0 0.0 0.0 0.057544597062723614 0.0 28 0.0 0.0 0.0 0.06254847506817784 0.0 29 0.0 0.0 0.0 0.08756786509544898 0.0 30 0.0 0.0 0.0 0.11508919412544723 0.0 31 0.0 0.0 0.0 0.13510470614726414 0.0 32 0.0 0.0 0.0 0.16012409617453527 0.0 33 0.0 0.0 0.0 0.19264930320998774 0.0 34 0.0 0.0 0.0 0.2401861442618029 0.0 35 0.0 0.0 0.0 0.33025594835997896 0.0 36 0.0 0.0 0.0 0.42783156946633644 0.0 37 0.0 0.0 0.0 0.6279866896845054 0.0 38 0.0 0.0 0.0 0.8356476269108559 0.0 39 0.0 0.0 0.0 1.098351222197203 0.0 40 0.0 0.0 0.0 1.4911556456253596 0.0 41 0.0 0.0 0.0 2.0090570191898722 0.0 42 0.0 0.0 0.0 2.689584427931647 0.0 43 0.0 0.0 0.0 3.3475943856488777 0.0 44 0.0 0.0 0.0 3.9755810753333836 0.0 45 0.0 0.0 0.0 4.528509594936075 0.0 46 0.0 0.0 0.0 5.11396332157422 0.0 47 0.0 0.0 0.0 5.654382146163276 0.0 48 0.0 0.0 0.0 6.097225349645976 0.0 49 0.0 0.0 0.0 6.530060797117766 0.0 50 0.0 0.0 0.0 6.990417573619555 0.0 51 0.0 0.0 0.0 7.520828642197703 0.0 52 0.0 0.0 0.0 8.201356050939479 0.0 53 0.0 0.0 0.0 9.021992043833972 0.0 54 0.0 0.0 0.0 9.7075233305812 0.0 55 0.0 0.0 0.0 10.348019715279342 0.0 56 0.0 0.0 0.0 10.880932722860218 0.0 57 0.0 0.0 0.0 11.361305011383822 0.0 58 0.0 0.0 0.0 11.94425679901924 0.0 59 0.0 0.0 0.0 12.344567039455578 0.0 60 0.0 0.0 0.0 12.812429632965548 0.0 61 0.0 0.0 0.0 13.292801921489154 0.0 62 0.0 0.0 0.0 13.763166454001851 0.0 63 0.0 0.0 0.0 14.358627936650905 0.0 64 0.0 0.0 0.0 14.966599114313594 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCTGG 15 0.0022102722 69.991234 20 CCTGGTT 15 0.0022102722 69.991234 22 GTTAGGC 20 0.0069066826 52.493427 55 CTGGTTC 20 0.0069066826 52.493427 23 TGGTTCA 20 0.0069066826 52.493427 24 GAAAGAT 20 0.0069066826 52.493427 39 ATAAGCG 20 0.0069066826 52.493427 70 CATACTT 20 0.0069066826 52.493427 22 GCAGCCT 20 0.0069066826 52.493427 8 GATTCTC 20 0.0069066826 52.493427 34 TGCAGCA 20 0.0069066826 52.493427 67 TATAAGC 20 0.0069066826 52.493427 69 TAGCTGA 20 0.0069066826 52.493427 59 ACCACAC 150 8.203642E-10 25.663452 2 CACACCT 95 2.7191773E-4 22.102495 4 ACACCTC 80 0.0026246898 21.872263 5 AACACAC 120 5.5481883E-5 20.41411 2 GACGAGG 185 4.528285E-6 17.024897 70 CGACGAG 195 7.4724358E-6 16.151825 69 AACCACA 135 0.0027180866 15.691078 1 >>END_MODULE