FastQCFastQC Report
Wed 25 May 2016
SRR1780388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39969
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT22905.72944031624509TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT12133.0348520103079886No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC9102.2767644924816732No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC2980.7455778228126798No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT1760.44034126447997196No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1150.28772298531361806No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT1050.26270359528634696No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT910.22767644924816732No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG850.21266481523180464No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC800.20015512021816909No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT780.19515124221271488No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC780.19515124221271488No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC760.19014736420726064No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT740.1851434862018064No Hit
CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC600.15011634016362682No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG580.14511246215817258No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC500.1250969501363557No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT490.12259501113362856No Hit
TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC490.12259501113362856No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT460.11508919412544721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCC150.002209175170.09
CCACTAG150.002209175170.02
CACTAGG150.002209175170.03
GGGCGCT150.002209175170.028
CTACCCA150.002209175170.022
AATTAGC150.002209175170.08
GTGGTGG150.002209175170.020
GAGGGCG150.002209175170.041
CTATACA352.9033617E-760.0000041
GAAAAAC352.9033617E-760.00000460
TCACATC252.3631127E-456.0000045
CTTTACA1650.053.0303041
TTACACA1650.053.0303043
GATACCA200.00690326652.59
TACCAAC200.00690326652.511
CAAAAAT200.00690326652.54
GGATGGA200.00690326652.56
GGCGTGG200.00690326652.517
AGCCAGG200.00690326652.512
CATCTGA200.00690326652.51
Produced by FastQC (version 0.11.5)