##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39969 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32004803722885 34.0 34.0 34.0 27.0 34.0 2 33.09074532762891 34.0 34.0 34.0 32.0 34.0 3 33.32968050238935 34.0 34.0 34.0 33.0 34.0 4 33.3996347169056 34.0 34.0 34.0 33.0 34.0 5 33.4205509269684 34.0 34.0 34.0 34.0 34.0 6 37.09077034701894 38.0 37.0 38.0 36.0 38.0 7 36.685731441867446 38.0 37.0 38.0 34.0 38.0 8 36.651179664239784 38.0 37.0 38.0 34.0 38.0 9 36.43328579649228 38.0 37.0 38.0 34.0 38.0 10-11 36.0797242863219 38.0 36.5 38.0 32.5 38.0 12-13 35.92132652805924 38.0 36.5 38.0 32.0 38.0 14-15 36.12530961495159 38.0 36.5 38.0 33.0 38.0 16-17 36.28055493007081 38.0 37.0 38.0 33.5 38.0 18-19 36.33325827516325 38.0 37.0 38.0 34.0 38.0 20-21 36.0554179489104 38.0 37.0 38.0 32.5 38.0 22-23 35.98617678700994 38.0 36.5 38.0 32.5 38.0 24-25 35.04951337286397 37.5 35.0 38.0 28.5 38.0 26-27 35.20806124746679 37.0 35.5 38.0 29.0 38.0 28-29 35.006417473542 37.5 35.5 38.0 29.0 38.0 30-31 34.574970602216716 37.0 34.0 38.0 26.5 38.0 32-33 35.25334634341615 37.0 35.5 38.0 30.5 38.0 34-35 34.98468813330331 37.0 35.5 38.0 27.0 38.0 36-37 35.06033425905076 37.5 35.5 38.0 27.0 38.0 38-39 34.65081688308439 37.0 35.0 38.0 26.5 38.0 40-41 34.47281643273537 37.0 34.0 38.0 26.0 38.0 42-43 35.15745953113663 37.0 35.5 38.0 29.0 38.0 44-45 35.45731692061348 37.5 36.0 38.0 31.0 38.0 46-47 35.65951112111887 38.0 36.0 38.0 31.0 38.0 48-49 35.349020490880434 37.5 36.0 38.0 29.0 38.0 50-51 35.52819685256074 38.0 36.0 38.0 31.0 38.0 52-53 35.378605919587685 38.0 36.0 38.0 31.0 38.0 54-55 35.60760839650729 38.0 36.0 38.0 31.0 38.0 56-57 35.64177237358953 38.0 36.0 38.0 31.5 38.0 58-59 35.65813505466737 38.0 36.0 38.0 31.0 38.0 60-61 35.30957742250244 38.0 36.0 38.0 29.0 38.0 62-63 35.747867097000174 38.0 36.0 38.0 31.5 38.0 64-65 35.74197503064875 38.0 36.5 38.0 31.5 38.0 66-67 34.699779829367756 37.0 34.5 38.0 26.0 38.0 68-69 33.04833746153268 37.0 32.5 38.0 21.5 38.0 70-71 32.11829167604894 37.0 29.5 38.0 15.0 38.0 72-73 31.230778853611547 37.0 26.0 38.0 11.0 38.0 74-75 31.702794665866048 37.0 27.5 38.0 11.0 38.0 76 31.28109284695639 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9530698499151313 1101 2 0.8097566913915983 1101 3 0.3898022001671393 1101 4 0.371563834437346 1101 5 0.16573946592036037 1101 6 -0.41970465957466274 1101 7 0.606683942640295 1101 8 1.1034644356991592 1101 9 0.9594799329121386 1101 10-11 0.7658204213668114 1101 12-13 1.6118198572084381 1101 14-15 1.2286284718919873 1101 16-17 0.3686457158986798 1101 18-19 -0.07251927311908446 1101 20-21 0.31074964051067155 1101 22-23 0.9092546869046814 1101 24-25 -1.3187954041392587 1101 26-27 0.2879285144467332 1101 28-29 1.832798518390689 1101 30-31 1.9114439009646844 1101 32-33 1.3568479693324633 1101 34-35 1.516413337354841 1101 36-37 1.3785579920897106 1101 38-39 1.4551572495166099 1101 40-41 1.6502716777888935 1101 42-43 1.3725097321515847 1101 44-45 1.1408316806416607 1101 46-47 1.024123959744209 1101 48-49 -0.6436391829571235 1101 50-51 -0.8705580879617116 1101 52-53 -0.8253396843771483 1101 54-55 -0.12918569829441395 1101 56-57 -0.4161232063910134 1101 58-59 -0.019147206491687996 1101 60-61 -0.9952243280832533 1101 62-63 -0.17393568013161342 1101 64-65 -0.4256851101213215 1101 66-67 -6.544299263921971E-4 1101 68-69 0.2372533100091445 1101 70-71 0.7696500966341482 1101 72-73 -0.18797002406646968 1101 74-75 0.4235421598681164 1101 76 1.6079459362023698 1102 1 -0.7847989121471812 1102 2 0.2016783897649077 1102 3 0.516835561071467 1102 4 0.2849219816664643 1102 5 0.3445373705247192 1102 6 0.4886217810098401 1102 7 0.4228543313913278 1102 8 1.009860906611756 1102 9 0.909369648930884 1102 10-11 0.8277173058443381 1102 12-13 1.3617853934642952 1102 14-15 0.37020553282388846 1102 16-17 0.678336920751164 1102 18-19 0.0795347660317276 1102 20-21 1.2087714215969712 1102 22-23 1.2190837467337374 1102 24-25 -4.504486057571846 1102 26-27 -0.6258776614562152 1102 28-29 1.1210532743873785 1102 30-31 2.53368680143339 1102 32-33 1.218303717884993 1102 34-35 1.2214036875064735 1102 36-37 -2.6138944479433732 1102 38-39 0.5531376741099407 1102 40-41 1.7882645093301122 1102 42-43 1.5237711051871514 1102 44-45 1.727680315877393 1102 46-47 1.7495513101715616 1102 48-49 -2.8841616533183014 1102 50-51 0.3701711648643169 1102 52-53 0.85466445127215 1102 54-55 1.277176308874047 1102 56-57 1.7532316323186663 1102 58-59 0.7682804196456203 1102 60-61 -5.094445447465663 1102 62-63 -2.1292362039805184 1102 64-65 0.666781115409421 1102 66-67 1.5425975689707698 1102 68-69 -0.8709628465941037 1102 70-71 -0.9955484145321094 1102 72-73 -5.225190867738927 1102 74-75 -1.9836814409038759 1102 76 -1.6640419325596945 1103 1 1.2185613029065792 1103 2 0.6105045546394621 1103 3 0.22046459332953106 1103 4 0.3566065694800855 1103 5 0.389564252245151 1103 6 0.46330816093815486 1103 7 1.1419403528967038 1103 8 0.9939558886906141 1103 9 0.6224072833394843 1103 10-11 0.3002755495719356 1103 12-13 -0.4731160402274597 1103 14-15 0.020295138558651615 1103 16-17 0.19289785265080894 1103 18-19 0.8209102140603974 1103 20-21 0.06529023880127482 1103 22-23 0.7260281911781803 1103 24-25 -2.449938566532417 1103 26-27 -0.14155866504043502 1103 28-29 0.03872800557017797 1103 30-31 1.6547665505373317 1103 32-33 1.5846791659136628 1103 34-35 0.8470757305172256 1103 36-37 -0.8468693583376421 1103 38-39 -0.11722522911586708 1103 40-41 0.1398550111222221 1103 42-43 0.055469133860988507 1103 44-45 1.7313765524365294 1103 46-47 1.4266346435048902 1103 48-49 -1.4946007214186636 1103 50-51 -0.214842275995899 1103 52-53 0.2740192899818297 1103 54-55 -0.08404680940552822 1103 56-57 0.8234601269423223 1103 58-59 -0.1850125911070677 1103 60-61 -3.9458119349208616 1103 62-63 -1.2620767057726354 1103 64-65 0.24204993261371754 1103 66-67 -0.6109642589862219 1103 68-69 0.43784091684675985 1103 70-71 0.49694710518115315 1103 72-73 -4.44304481039126 1103 74-75 -2.9463830538071 1103 76 -3.8867121834557494 1104 1 0.8329198160960303 1104 2 0.4923490790199594 1104 3 0.6750509242647809 1104 4 -0.3770065400323048 1104 5 -0.6474561314478962 1104 6 -0.15874419717535204 1104 7 0.3143024632084561 1104 8 0.8266397416082754 1104 9 1.1900032066008777 1104 10-11 -0.4912662170147968 1104 12-13 0.6528332003800585 1104 14-15 0.788980190517087 1104 16-17 0.401880666399812 1104 18-19 0.749991948672708 1104 20-21 0.06851266086361818 1104 22-23 0.5482204373812181 1104 24-25 1.1323206261134615 1104 26-27 1.5984493285646693 1104 28-29 2.3403616986157374 1104 30-31 1.3543818582911271 1104 32-33 0.9750918357777749 1104 34-35 -0.18994465317581444 1104 36-37 1.2779308015344668 1104 38-39 1.6132154183661456 1104 40-41 2.1522700790678684 1104 42-43 0.8001983504876904 1104 44-45 1.3159838665852135 1104 46-47 1.2882203749197672 1104 48-49 0.14243964628717265 1104 50-51 1.000160721606015 1104 52-53 0.16340777919046445 1104 54-55 0.138600073088476 1104 56-57 0.7745242756233708 1104 58-59 -0.34740767503992487 1104 60-61 0.40952879249720553 1104 62-63 -0.03730652191972439 1104 64-65 0.6002633618703186 1104 66-67 0.05997399041426377 1104 68-69 1.3824602214031145 1104 70-71 0.8205632122954647 1104 72-73 0.027303910631921724 1104 74-75 -0.8117157876200487 1104 76 -1.4341742138314508 1105 1 1.061542287246624 1105 2 0.4858308037073229 1105 3 0.4339713047389253 1105 4 0.10138658439432646 1105 5 0.0952409860259067 1105 6 0.4798536493796206 1105 7 1.0147032164313785 1105 8 -0.03787669893056744 1105 9 1.1667305495587286 1105 10-11 0.7601702177300282 1105 12-13 -0.5369210364988959 1105 14-15 0.7226627746511198 1105 16-17 0.4140481136500611 1105 18-19 0.4944514146569645 1105 20-21 0.13946529472968194 1105 22-23 0.3080099212303864 1105 24-25 0.8927860792543143 1105 26-27 0.3493063610594831 1105 28-29 1.6197542919236412 1105 30-31 1.0457294513576159 1105 32-33 0.9855693597988235 1105 34-35 -1.141878408185228 1105 36-37 0.6239546535937563 1105 38-39 0.3792093346425176 1105 40-41 0.7591456524346825 1105 42-43 0.4345472472092382 1105 44-45 0.9846623466199773 1105 46-47 0.8644841960776048 1105 48-49 -0.4580142188086711 1105 50-51 0.9604233839593519 1105 52-53 -0.001987421427564584 1105 54-55 0.9431483807510759 1105 56-57 0.5670982701190539 1105 58-59 0.20473852746115995 1105 60-61 0.8435010777562439 1105 62-63 -0.408003253125365 1105 64-65 0.558884384515288 1105 66-67 0.2933282466066274 1105 68-69 1.0801088139384731 1105 70-71 1.6916944951886101 1105 72-73 0.8396500069092632 1105 74-75 0.7232897649848518 1105 76 0.8253497106531213 1106 1 -1.3886053637600853 1106 2 0.16722677686168197 1106 3 0.5109645933295326 1106 4 -0.14494898607546958 1106 5 0.2747309189118141 1106 6 0.5083637164937116 1106 7 -0.11372631376995912 1106 8 0.5973447775795009 1106 9 -0.684759383327183 1106 10-11 1.0051366606830499 1106 12-13 1.8334950708836502 1106 14-15 -0.14114930588579 1106 16-17 0.5071756304285913 1106 18-19 0.7404379918381778 1106 20-21 0.6117069054679405 1106 22-23 0.5250837467337348 1106 24-25 0.08725587791202116 1106 26-27 1.3902191127373413 1106 28-29 1.0413113389035118 1106 30-31 0.9654609949817754 1106 32-33 0.031206943691444167 1106 34-35 1.5402423971838957 1106 36-37 1.3836861972179122 1106 38-39 0.22452477088413048 1106 40-41 0.8947161222333335 1106 42-43 0.9439969116387701 1106 44-45 1.3312932191032019 1106 46-47 1.2425513101715566 1106 48-49 1.4756770563591175 1106 50-51 0.05207439067076791 1106 52-53 1.0730192899818292 1106 54-55 -0.10963014273885818 1106 56-57 0.5358767936089848 1106 58-59 0.8175707422262661 1106 60-61 1.479715842856919 1106 62-63 0.8773121831162527 1106 64-65 -0.652283400719611 1106 66-67 1.000952407680451 1106 68-69 2.6574242501800924 1106 70-71 1.6804193274033743 1106 72-73 1.6172607451642982 1106 74-75 0.5218669461928975 1106 76 0.2047322609886919 1107 1 1.4035737407175262 1107 2 -0.05999710373533418 1107 3 0.6635616082549021 1107 4 0.45403608855139055 1107 5 0.1858950980162959 1107 6 0.5309905821653516 1107 7 0.9472886116031773 1107 8 0.005046270116814355 1107 9 -0.5582519206406147 1107 10-11 0.6208083024740958 1107 12-13 0.22474880222693372 1107 14-15 0.15607457471122643 1107 16-17 0.6339218990853084 1107 18-19 0.6327364993008615 1107 20-21 0.6391397412888296 1107 22-23 0.03251658255462786 1107 24-25 0.42221110179261956 1107 26-27 0.34517433661793007 1107 28-29 1.2839083538288776 1107 30-31 0.7330729352802834 1107 32-33 0.9319532123481551 1107 34-35 0.2814662777809147 1107 36-37 1.315029480800007 1107 38-39 0.22652477088413292 1107 40-41 0.41934298790498303 1107 42-43 0.8915491504447317 1107 44-45 1.0598902340285719 1107 46-47 1.053655787783505 1107 48-49 0.9053785488964294 1107 50-51 1.5521042414170338 1107 52-53 0.9512580959519781 1107 54-55 1.027116125917857 1107 56-57 1.3338767936089866 1107 58-59 0.4706155183456673 1107 60-61 1.4966113652449735 1107 62-63 0.6221480040117697 1107 64-65 0.8620748082356116 1107 66-67 -0.036181920677755386 1107 68-69 2.252051115851735 1107 70-71 1.29892679008994 1107 72-73 1.8163353720299646 1107 74-75 -0.3946106657473969 1107 76 0.6864636042722765 1108 1 -0.023195291817636132 1108 2 0.23335627326456176 1108 3 0.315338694048954 1108 4 0.36400065421229755 1108 5 0.28060142250893705 1108 6 -0.2954348446573647 1108 7 0.39343915385593675 1108 8 -0.6985401145068337 1108 9 0.21158594041382628 1108 10-11 0.9188057254312554 1108 12-13 1.5557108982217827 1108 14-15 0.9112823487904649 1108 16-17 0.27957850812643414 1108 18-19 0.5269703659388938 1108 20-21 1.4066421572665035 1108 22-23 0.49066648054668605 1108 24-25 1.7438314174803722 1108 26-27 0.6092406954711507 1108 28-29 0.45187248998264806 1108 30-31 1.1925113546940054 1108 32-33 1.435523490454031 1108 34-35 1.2165301669680701 1108 36-37 1.8498732475776265 1108 38-39 1.2750859219632744 1108 40-41 1.2853636042477206 1108 42-43 0.5124285663150232 1108 44-45 1.582170916944925 1108 46-47 1.3601628209629268 1108 48-49 1.4841662649922043 1108 50-51 1.2159880597355084 1108 52-53 0.5051343978954961 1108 54-55 -0.04125604201943389 1108 56-57 0.7493444195082688 1108 58-59 0.9007937638089913 1108 60-61 1.2512554112022372 1108 62-63 1.441110744267327 1108 64-65 0.9815583258990941 1108 66-67 1.890909242212821 1108 68-69 2.6018846818347683 1108 70-71 0.7594121331587722 1108 72-73 -0.023055801598292902 1108 74-75 -0.6462481617207665 1108 76 0.6161854983987638 1109 1 -0.3693010159339991 1109 2 0.27203837106458195 1109 3 0.5728196657932969 1109 4 0.1090220284172787 1109 5 0.3071367160132681 1109 6 -0.35523048640483523 1109 7 -0.2874074731902496 1109 8 -0.14161174415963274 1109 9 0.8606319210206479 1109 10-11 0.5486149215526126 1109 12-13 0.44506028827495214 1109 14-15 0.8095463462881227 1109 16-17 0.6817263550662673 1109 18-19 0.7214234990845512 1109 20-21 1.151793861989674 1109 22-23 -0.28453944167205947 1109 24-25 -0.5077586148415989 1109 26-27 0.47317563447646194 1109 28-29 -0.5677031538501183 1109 30-31 0.9112580964310553 1109 32-33 0.5667141900682537 1109 34-35 -0.6411489071639309 1109 36-37 1.3264398204063212 1109 38-39 1.4656552056667422 1109 40-41 0.3266001802043519 1109 42-43 1.2295331435228292 1109 44-45 0.2952352480887086 1109 46-47 0.9035368174179368 1109 48-49 0.8601698099823025 1109 50-51 -0.18178068179300055 1109 52-53 -0.9488067969746865 1109 54-55 -0.19319536013016148 1109 56-57 0.02590577911623626 1109 58-59 0.21217943787843296 1109 60-61 0.9075999008279325 1109 62-63 -0.16576028065186676 1109 64-65 0.711252831164451 1109 66-67 0.2934741468108868 1109 68-69 -0.8551844454720836 1109 70-71 0.5112888926207653 1109 72-73 -0.3426522983139648 1109 74-75 -2.7798134333423263E-4 1109 76 -0.4585720868373926 1110 1 -2.6589104485058463 1110 2 -0.9522308502569601 1110 3 -0.37805235582300867 1110 4 -0.3472540708212364 1110 5 0.2712732917931717 1110 6 -0.28939899537069635 1110 7 -0.005658517159794485 1110 8 0.8351752860540742 1110 9 0.8720541759948546 1110 10-11 -0.36774469524915787 1110 12-13 0.5500713420700976 1110 14-15 1.0186473042837036 1110 16-17 0.829785799920117 1110 18-19 0.2602346020076709 1110 20-21 0.9951984308916693 1110 22-23 -0.7809162532662626 1110 24-25 -0.8375915797150881 1110 26-27 1.4243547059576755 1110 28-29 0.4075486270390982 1110 30-31 -0.3928440897639831 1110 32-33 0.6871391470812682 1110 34-35 0.9872593463364367 1110 36-37 1.8548726378958804 1110 38-39 1.3621179912231156 1110 40-41 -1.3925720133598816 1110 42-43 0.1367426743506286 1110 44-45 1.2725135580862528 1110 46-47 1.3533648694935962 1110 48-49 1.5914397682235162 1110 50-51 -0.0656544228885565 1110 52-53 0.4159684425242034 1110 54-55 0.20603087421029187 1110 56-57 1.1093005224225436 1110 58-59 -0.3373784103161128 1110 60-61 0.7435124530264119 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-0.025223134260592417 2116 54-55 -1.5509028700115906 2116 56-57 -1.3403656306334355 2116 58-59 -2.96436865171313 2116 60-61 -2.1197387025976298 2116 62-63 0.30294854675261007 2116 64-65 -4.511737946174158 2116 66-67 -1.9812294105013635 2116 68-69 -2.6028787801229427 2116 70-71 -1.135944308960262 2116 72-73 -1.6990422851387343 2116 74-75 -1.3961936598677056 2116 76 -1.4309041026476699 2117 1 -0.07598997914470118 2117 2 0.09522677686168635 2117 3 0.09065690102183765 2117 4 -0.0027951399216235018 2117 5 0.26488476506565917 2117 6 0.002517562647561533 2117 7 0.3656583016146584 2117 8 -0.38542445318972796 2117 9 -1.5721439987117947 2117 10-11 -0.7564018008554143 2117 12-13 -2.297581852193268 2117 14-15 -0.43376469050117805 2117 16-17 -1.0706705234175686 2117 18-19 -2.04602354662336 2117 20-21 -1.2319854022243675 2117 22-23 -0.40783933018933993 2117 24-25 -2.4290518143956703 2117 26-27 -2.5753193488011235 2117 28-29 -3.5928425072503387 2117 30-31 -1.0483851588643773 2117 32-33 -4.199562287077789 2117 34-35 0.007011627953126265 2117 36-37 -2.900929187397473 2117 38-39 -0.24655215219279114 2117 40-41 -0.3822069546897424 2117 42-43 -0.2864646268227702 2117 44-45 -1.9514760116660312 2117 46-47 -2.171602535982281 2117 48-49 -0.9581690974870369 2117 50-51 -1.0150025324061573 2117 52-53 0.033634674597216474 2117 54-55 -0.7351686042773196 2117 56-57 -0.685353975621787 2117 58-59 -2.7781215654660514 2117 60-61 -1.8473610802200113 2117 62-63 -0.8645339707299016 2117 64-65 -1.3239757084119148 2117 66-67 -2.2553552846272424 2117 68-69 -0.7764219036660691 2117 70-71 -0.5303499033658561 2117 72-73 1.4351068990104459 2117 74-75 1.2252515615775188 2117 76 0.47119379945023354 2118 1 -0.565690109522798 2118 2 0.4037013531328668 2118 3 -1.9966964236196212 2118 4 -0.008271019973776106 2118 5 -0.09313348786784559 2118 6 -0.12838204621814953 2118 7 0.5028160591113959 2118 8 0.1911074894439082 2118 9 -2.424555993496675 2118 10-11 -2.062659949486438 2118 12-13 -1.9202676409807538 2118 14-15 0.04407103309726068 2118 16-17 -3.725298945842596 2118 18-19 -1.3160365844330073 2118 20-21 -1.4666659758879916 2118 22-23 0.2911176450388169 2118 24-25 0.3107135050306695 2118 26-27 -3.588357158449103 2118 28-29 -2.0331293390625973 2118 30-31 -1.172505106713139 2118 32-33 -0.7365896664780536 2118 34-35 -0.8813847214601722 2118 36-37 -4.2637714299007285 2118 38-39 -1.3031362460650229 2118 40-41 -1.9561313353937848 2118 42-43 -0.9225793595476688 2118 44-45 -4.829181357167986 2118 46-47 -1.1339232660996217 2118 48-49 -0.522967011437494 2118 50-51 -1.4766713720410962 2118 52-53 -2.079794269340205 2118 54-55 -1.361765735959196 2118 56-57 -0.7296825284249095 2118 58-59 -2.6805987492991648 2118 60-61 -2.6632672079905433 2118 62-63 -2.132721715188829 2118 64-65 -0.5806901803806284 2118 66-67 -0.6630475923195505 2118 68-69 -3.051389309141946 2118 70-71 0.46652102231862713 2118 72-73 2.445667524825314 2118 74-75 2.1759686411081525 2118 76 1.1022237864124236 2119 1 0.5251089219541996 2119 2 -1.5333446517097435 2119 3 -0.07417826381332304 2119 4 -0.04418708131356652 2119 5 -0.5717452715643745 2119 6 -0.8055410454110472 2119 7 0.06455940051575482 2119 8 -0.17370284146812054 2119 9 -1.5472355738033698 2119 10-11 -1.8758157202693297 2119 12-13 -3.0911715957830097 2119 14-15 -1.2634350201714994 2119 16-17 -0.5263481790952227 2119 18-19 -0.4266096272094444 2119 20-21 -3.781435951674915 2119 22-23 -1.352344824694832 2119 24-25 -0.25102983637368936 2119 26-27 -2.530447553929328 2119 28-29 -0.822879137286975 2119 30-31 0.02927051879130005 2119 32-33 -0.02593591345141988 2119 34-35 -1.328519507578001 2119 36-37 -1.1782185646868442 2119 38-39 -1.9163323719730059 2119 40-41 -0.6671886396714228 2119 42-43 -1.0194316597898023 2119 44-45 -0.32070678089679916 2119 46-47 -1.006401070780818 2119 48-49 -1.3090848484027902 2119 50-51 -0.2728779902816143 2119 52-53 -1.0057426147800754 2119 54-55 -0.5379158570245721 2119 56-57 -3.154218444486254 2119 58-59 -0.4037625911070677 2119 60-61 -0.6099984428573748 2119 62-63 -0.774973531169465 2119 64-65 -0.013902448338662055 2119 66-67 0.8250476457756903 2119 68-69 -1.4892424164865794 2119 70-71 -3.0829140059299576 2119 72-73 -0.40683449293094043 2119 74-75 -0.8139425776166256 2119 76 0.07558940384583579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 7.0 23 19.0 24 28.0 25 75.0 26 159.0 27 285.0 28 498.0 29 811.0 30 1248.0 31 1929.0 32 2640.0 33 3723.0 34 5285.0 35 6162.0 36 7851.0 37 9247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.809652480672522 25.167004428432033 11.03355100202657 31.989792088868874 2 16.232580249693513 18.699492106382447 44.54452200455353 20.523405639370512 3 15.572068352973552 27.956666416472764 35.42745627861593 21.043808951937752 4 10.790862918762041 22.092121394080415 35.74520253196227 31.371813155195277 5 10.433085641372063 40.97675698666466 35.38992719357502 13.20023017838825 6 21.516675423453176 37.361455127723985 16.610373039105305 24.51149640971753 7 21.414095924341364 38.79756811528935 18.64444944832245 21.143886512046837 8 18.74702894743426 34.63684355375416 24.66661662788661 21.949510870924968 9 19.13983337086242 21.25397182816683 18.19159848882884 41.41459631214191 10-11 19.39002727113513 24.771698066001154 30.30098326202807 25.53729140083565 12-13 22.905251569966726 19.891666041181917 27.173559508619178 30.02952288023218 14-15 23.912282018564387 24.21001275988891 20.615977382471414 31.26172783907528 16-17 22.93902774650354 27.282393855237807 20.866171282744126 28.912407115514522 18-19 20.764842753133678 27.552603267532337 21.586729715529536 30.09582426380445 20-21 21.206434987115014 25.20703545247567 21.496659911431358 32.08986964897796 22-23 23.840976756986663 29.95446471015037 21.48289924691636 24.72165928594661 24-25 22.906502539468086 32.3150441592234 21.606745227551354 23.17170807375716 26-27 25.029397783282043 28.40451349796092 25.594835997898368 20.971252720858665 28-29 26.82203707873602 23.08414020866171 28.826090219920435 21.26773249268183 30-31 31.869699016737975 21.663038855112713 27.84157722234732 18.625684905801997 32-33 29.520378293177213 22.349821111361305 30.40856663914534 17.721233956316144 34-35 24.4026620630989 26.295378918661967 31.67329680502389 17.628662213215243 36-37 21.26898346218319 26.872075858790563 29.68675723685856 22.172183442167682 38-39 21.39783332082364 24.157472040831646 27.466286371938253 26.97840826640647 40-41 19.54139458080012 25.781230453601538 30.484875778728515 24.192499186869824 42-43 24.332607771022545 23.865996147013934 32.19870399559659 19.602692086366936 44-45 19.35124721659286 24.463959568665715 29.253921789386773 26.930871425354653 46-47 23.976081463133927 20.985013385373666 28.58090019765318 26.458004953839225 48-49 21.237709224649105 20.84865770972504 30.36978658460307 27.543846481022793 50-51 16.9881658285171 21.101353549000475 33.74740423828467 28.163076384197755 52-53 19.2811929245165 21.19017238359729 36.81227951662539 22.716355175260826 54-55 17.990192399109308 21.48289924691636 33.88751282243739 26.63939553153694 56-57 22.572493682604016 21.31401836423228 31.235457479546646 24.878030473617052 58-59 18.11779128824839 29.41154394655858 31.67830068302935 20.79236408216368 60-61 18.54562285771473 37.38021967024444 24.51149640971753 19.5626610623233 62-63 15.440716555330381 42.14641347043959 22.42487928144312 19.987990692786912 64-65 15.288098276164028 43.15469488853862 21.2789912181941 20.278215617103253 66-67 15.166754234531762 42.31654532262503 21.177662688583652 21.33903775425955 68-69 15.094198003452675 39.770822387350194 21.65553303810453 23.479446571092595 70-71 15.640871675548551 37.01493657584628 22.46115739698266 24.883034351622506 72-73 16.555330381045312 34.396657409492356 22.761390077309915 26.28662213215242 74-75 17.34594310590708 33.62105631864695 22.7138532362581 26.31914733918787 76 18.32169931697065 34.0313743150942 22.354824989366758 25.29210137856839 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.0 14 0.0 15 0.0 16 1.0 17 2.0 18 3.0 19 7.0 20 13.5 21 17.0 22 22.5 23 34.5 24 45.0 25 49.0 26 84.5 27 131.0 28 190.5 29 239.0 30 271.0 31 338.5 32 467.0 33 560.0 34 638.5 35 813.0 36 980.5 37 1052.0 38 1208.0 39 1527.5 40 1957.5 41 2771.5 42 3319.0 43 3745.5 44 4204.0 45 3998.0 46 3760.0 47 3426.5 48 2793.0 49 2274.0 50 2055.0 51 1847.5 52 1473.0 53 1185.5 54 1065.0 55 996.5 56 809.0 57 588.5 58 487.0 59 404.0 60 265.0 61 185.0 62 161.0 63 120.5 64 63.5 65 40.5 66 24.5 67 15.0 68 9.5 69 4.5 70 2.5 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 39969.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.886587105006384 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81035316884373 43.12592259000726 2 13.85846857544971 15.767219595186269 3 5.150195716233452 8.78931171658035 4 2.212253155649382 5.033901273486952 5 0.9499934028235915 2.7020941229452826 6 0.6817082288780402 2.3268032725362158 7 0.37823811408717073 1.5061672796417225 8 0.24189646831156267 1.10085316119993 9 0.12754541056427848 0.6530060797117767 >10 0.5189778774684436 4.5159998999224396 >50 0.039583058450982984 1.701318521854437 >100 0.0175924704226591 1.7363456678926168 >500 0.004398117605664775 2.2767644924816732 >1k 0.00879623521132955 8.764292326553079 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT 2290 5.72944031624509 TruSeq Adapter, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 1213 3.0348520103079886 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 910 2.2767644924816732 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 298 0.7455778228126798 No Hit TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT 176 0.44034126447997196 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 115 0.28772298531361806 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT 105 0.26270359528634696 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT 91 0.22767644924816732 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 85 0.21266481523180464 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 80 0.20015512021816909 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT 78 0.19515124221271488 No Hit CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 78 0.19515124221271488 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 76 0.19014736420726064 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT 74 0.1851434862018064 No Hit CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 60 0.15011634016362682 No Hit CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 58 0.14511246215817258 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 50 0.1250969501363557 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT 49 0.12259501113362856 No Hit TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 49 0.12259501113362856 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT 46 0.11508919412544721 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005003878005454227 0.0 2 0.0 0.0 0.0 0.005003878005454227 0.0 3 0.0 0.0 0.0 0.005003878005454227 0.0 4 0.0 0.0 0.0 0.007505817008181341 0.0 5 0.0 0.0 0.0 0.007505817008181341 0.0 6 0.0 0.0 0.0 0.007505817008181341 0.0 7 0.0 0.0 0.0 0.007505817008181341 0.0 8 0.0 0.0 0.0 0.007505817008181341 0.0 9 0.0 0.0 0.0 0.010007756010908454 0.0 10 0.0 0.0 0.0 0.012509695013635568 0.0 11 0.0 0.0 0.0 0.012509695013635568 0.0 12 0.0 0.0 0.0 0.012509695013635568 0.0 13 0.0 0.0 0.0 0.012509695013635568 0.0 14 0.0 0.0 0.0 0.012509695013635568 0.0 15 0.0 0.0 0.0 0.012509695013635568 0.0 16 0.0 0.0 0.0 0.012509695013635568 0.0 17 0.0 0.0 0.0 0.012509695013635568 0.0 18 0.0 0.0 0.0 0.015011634016362681 0.0 19 0.0 0.0 0.0 0.015011634016362681 0.0 20 0.0 0.0 0.0 0.015011634016362681 0.0 21 0.0 0.0 0.0 0.02001551202181691 0.0 22 0.0 0.0 0.0 0.02251745102454402 0.0 23 0.0 0.0 0.0 0.02251745102454402 0.0 24 0.0 0.0 0.0 0.027521329029998248 0.0 25 0.0 0.0 0.0 0.030023268032725363 0.0 26 0.0 0.0 0.0 0.04253296304636093 0.0 27 0.0 0.0 0.0 0.06254847506817784 0.0 28 0.0 0.0 0.0 0.07255623107908629 0.0 29 0.0 0.0 0.0 0.0925717431009032 0.0 30 0.0 0.0 0.0 0.12009307213090145 0.0 31 0.0 0.0 0.0 0.14261052315544548 0.0 32 0.0 0.0 0.0 0.16012409617453527 0.0 33 0.0 0.0 0.0 0.19014736420726064 0.0 34 0.0 0.0 0.0 0.2401861442618029 0.0 35 0.0 0.0 0.0 0.33025594835997896 0.0 36 0.0 0.0 0.0 0.4253296304636093 0.0 37 0.0 0.0 0.0 0.6505041407090495 0.0 38 0.0 0.0 0.0 0.8656708949435813 0.0 39 0.0 0.0 0.0 1.0958492831944757 0.0 40 0.0 0.0 0.0 1.4736420726062698 0.0 41 0.0 0.0 0.0 1.9915434461707824 0.0 42 0.0 0.0 0.0 2.6545572818934673 0.0 43 0.0 0.0 0.0 3.330080812629788 0.0 44 0.0 0.0 0.0 3.993094648352473 0.0 45 0.0 0.0 0.0 4.55102704596062 0.0 46 0.0 0.0 0.0 5.146488528609672 0.0 47 0.0 0.0 0.0 5.691911231204183 0.0 48 0.0 0.0 0.0 6.132252495684155 0.0 49 0.0 0.0 0.0 6.58760539418049 0.0 50 0.0 0.0 0.0 7.070479621706823 0.0 51 0.0 0.0 0.0 7.6159023243013335 0.0 52 0.0 0.0 0.0 8.316445245064925 0.0 53 0.0 0.0 0.0 9.152092871975782 0.0 54 0.0 0.0 0.0 9.865145487753008 0.0 55 0.0 0.0 0.0 10.50564187245115 0.0 56 0.0 0.0 0.0 11.031049063023843 0.0 57 0.0 0.0 0.0 11.516425229552903 0.0 58 0.0 0.0 0.0 12.096875078185594 0.0 59 0.0 0.0 0.0 12.49968725762466 0.0 60 0.0 0.0 0.0 12.977557607145538 0.0 61 0.0 0.0 0.0 13.460431834671871 0.0 62 0.0 0.0 0.0 13.918286672170932 0.0 63 0.0 0.0 0.0 14.496234581800895 0.0 64 0.0 0.0 0.0 15.10920963746904 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGCC 15 0.0022091751 70.0 9 CCACTAG 15 0.0022091751 70.0 2 CACTAGG 15 0.0022091751 70.0 3 GGGCGCT 15 0.0022091751 70.0 28 CTACCCA 15 0.0022091751 70.0 22 AATTAGC 15 0.0022091751 70.0 8 GTGGTGG 15 0.0022091751 70.0 20 GAGGGCG 15 0.0022091751 70.0 41 CTATACA 35 2.9033617E-7 60.000004 1 GAAAAAC 35 2.9033617E-7 60.000004 60 TCACATC 25 2.3631127E-4 56.000004 5 CTTTACA 165 0.0 53.030304 1 TTACACA 165 0.0 53.030304 3 GATACCA 20 0.006903266 52.5 9 TACCAAC 20 0.006903266 52.5 11 CAAAAAT 20 0.006903266 52.5 4 GGATGGA 20 0.006903266 52.5 6 GGCGTGG 20 0.006903266 52.5 17 AGCCAGG 20 0.006903266 52.5 12 CATCTGA 20 0.006903266 52.5 1 >>END_MODULE