FastQCFastQC Report
Wed 25 May 2016
SRR1780387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571135
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACA250.002344500535.2221724
CTTTTCG508.3279796E-526.40273536
AATGACG801.3115277E-724.7417229
CTATACA1350.024.4512631
GCTCTCG450.001402897824.42770641
TCGTTCG551.5882253E-424.008823
ACGTGAT551.5910614E-424.00248737
CGGTAAG551.5957982E-423.99197228
TTCGGTA551.5967469E-423.9898726
CGACAAT651.797519E-523.71555117
AACGATT651.806082E-523.70099422
GCATATA651.8073082E-523.6989151
TTTTCGC651.8097628E-523.69476137
CGTTCGG602.858005E-422.01385524
TTCGGTG703.2307296E-521.9830144
TGACGTG956.875307E-720.83513331
GACGTGA956.8811096E-720.83330732
TCGGTGG2650.020.74050542
TCGGTAA755.4839464E-520.52466627
ACGGGTT755.5061835E-520.5138825
Produced by FastQC (version 0.11.5)