FastQCFastQC Report
Wed 25 May 2016
SRR1780387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571135
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT325995.7077573603438765No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT308965.409579171299255TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC108451.89885053446208No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC48460.8484859096360755No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG36870.6455566547313683No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT27280.47764539031927655No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC17040.29835327899708475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC16440.28784788184930005No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG16250.2845211727525016No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC10270.17981738117958102No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9460.1656350950300717No Hit
CTTAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC8190.1433986710672608No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC7400.12956656482267767No Hit
CTTATCACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC7120.12466404615371147No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6480.1134582891960745No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG5800.10155217242858519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT31950.042.89469544
GCCGTCT35850.038.22832543
TTTACAC11800.037.8476072
CTATACA6250.036.2720261
CTTTACA12850.035.112791
GACGATG353.207429E-431.4424611
TTACACA14400.031.166793
CTTATAA1150.030.6222251
CTTATAC52250.030.202821
TTATACA52300.029.8242112
CCGGTCG703.583591E-828.2858269
TCGAGTA407.030993E-427.497741
GATCCTA508.3088446E-526.4116691
ACGTTAT508.3336374E-526.4001059
TATACAC59750.026.3632873
TCTTTAT2600.026.2423651
CGGTCGT609.714471E-625.67576210
TGTAGCG609.727686E-625.67126716
AAGTCGT609.754165E-625.66227519
TGCCGTC54000.025.42009742
Produced by FastQC (version 0.11.5)