FastQCFastQC Report
Wed 25 May 2016
SRR1780386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57922
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA207.868185E-569.9948225
GGGTCGG207.868185E-569.9948224
GTCGGAG207.868185E-569.9948226
CTCCGGA150.002214870469.9948112
TTGCTAA150.002214870469.9948127
CCGGATC150.002214870469.9948114
AGGTTGT150.002214870469.9948157
CGGATCC150.002214870469.9948115
GGGAACA150.002214870469.9948143
AAGGTTG308.284076E-658.32901436
AGGTGTT252.3729254E-455.99585743
ATTTATG200.006920933352.4961173
GGATCCA200.006920933352.49611716
TTGGGGA200.006920933352.49611740
TCTGCTT200.006920933352.49611760
GGGAGGG305.835143E-446.66320841
TTGATTT350.001246373339.9970466
AGGAAAA400.00240144434.9974131
GGAAAAG400.00240144434.9974132
AGGTTGG552.960016E-431.81582837
Produced by FastQC (version 0.11.5)