##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57922 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.407133731570042 34.0 23.0 34.0 12.0 34.0 2 29.37389938192742 34.0 27.0 34.0 12.0 34.0 3 29.568229688201374 34.0 31.0 34.0 11.0 34.0 4 30.107592969856015 34.0 31.0 34.0 11.0 34.0 5 30.165273989157832 34.0 31.0 34.0 11.0 34.0 6 32.22903214668002 37.0 31.0 38.0 11.0 38.0 7 31.340544180104278 36.0 27.0 38.0 11.0 38.0 8 30.762421877697594 36.0 26.0 38.0 11.0 38.0 9 31.88853976036739 37.0 31.0 38.0 12.0 38.0 10-11 31.964460826628915 37.0 31.0 38.0 11.0 38.0 12-13 32.253349331860086 37.0 31.0 38.0 11.0 38.0 14-15 32.042229204792655 37.0 31.0 38.0 11.0 38.0 16-17 31.861701943993644 37.0 31.0 38.0 11.0 38.0 18-19 31.898794931114256 37.0 29.5 38.0 11.0 38.0 20-21 31.994268153723972 37.0 31.0 38.0 11.0 38.0 22-23 31.838498325334072 37.0 29.5 38.0 11.0 38.0 24-25 32.24741031041746 37.0 31.0 38.0 11.0 38.0 26-27 32.22501812782708 37.0 31.0 38.0 11.0 38.0 28-29 32.261895307482476 37.0 31.0 38.0 11.0 38.0 30-31 32.18161493042368 37.0 31.0 38.0 11.0 38.0 32-33 31.977806360277615 37.0 29.0 38.0 11.0 38.0 34-35 32.0519491730258 37.0 31.0 38.0 11.0 38.0 36-37 32.011921204378304 37.0 31.0 38.0 11.0 38.0 38-39 32.277096785332 37.0 31.0 38.0 11.0 38.0 40-41 31.892130796588518 37.0 29.0 38.0 11.0 38.0 42-43 32.19314768136459 37.0 31.0 38.0 11.0 38.0 44-45 31.395652774420775 37.0 27.0 38.0 11.0 38.0 46-47 32.03279410241359 37.0 31.0 38.0 11.0 38.0 48-49 31.94045440419875 37.0 30.5 38.0 11.0 38.0 50-51 31.80535029867753 37.0 29.0 38.0 11.0 38.0 52-53 31.7463312730914 37.0 28.5 38.0 11.0 38.0 54-55 31.55648112979524 37.0 27.0 38.0 11.0 38.0 56-57 31.512301025517075 37.0 27.0 38.0 11.0 38.0 58-59 31.45165049549394 37.0 29.0 38.0 11.0 38.0 60-61 31.537619557335727 37.0 27.0 38.0 11.0 38.0 62-63 31.51639273505749 37.0 27.0 38.0 11.0 38.0 64-65 31.590008977590553 37.0 27.0 38.0 11.0 38.0 66-67 31.580383964642106 37.0 27.5 38.0 11.0 38.0 68-69 31.00023307206243 37.0 26.0 38.0 11.0 38.0 70-71 31.083612444321673 37.0 27.0 38.0 11.0 38.0 72-73 31.337004937674806 37.0 27.0 38.0 11.0 38.0 74-75 30.95436966955561 36.5 26.0 38.0 11.0 38.0 76 30.30178515935223 36.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.9816858019013708 1101 2 -0.7921501656588354 1101 3 -0.5115313115277615 1101 4 -2.3899607996613206 1101 5 -1.205696399274121 1101 6 -2.747374671269494 1101 7 -2.1497881258834113 1101 8 -0.9709405322726994 1101 9 -0.5063537401591915 1101 10-11 -1.010066134643651 1101 12-13 -1.4522573172217292 1101 14-15 -0.5585105892151034 1101 16-17 -1.446821679475601 1101 18-19 -0.7932353394470937 1101 20-21 -2.321574751429189 1101 22-23 -0.9664149583560331 1101 24-25 -1.744670636483832 1101 26-27 -1.3588909096084478 1101 28-29 -0.8182019377043659 1101 30-31 -1.4377757310801584 1101 32-33 -0.46638469422015305 1101 34-35 -0.6842535035529096 1101 36-37 -0.6412714855873318 1101 38-39 -1.2915024146479155 1101 40-41 -0.3549966692246045 1101 42-43 -0.7161772969484872 1101 44-45 -0.552268317973212 1101 46-47 0.9078983390817328 1101 48-49 -1.0400507659587177 1101 50-51 -0.25255541415561567 1101 52-53 -0.5318280470990331 1101 54-55 -1.3713041161508457 1101 56-57 -0.9225339212266057 1101 58-59 -0.20790746364689738 1101 60-61 -0.5456305160292736 1101 62-63 -1.2012377075734868 1101 64-65 -0.22699462780850155 1101 66-67 -1.1571466748038866 1101 68-69 -0.2909768035846447 1101 70-71 -1.0383643139327852 1101 72-73 -1.792939815672387 1101 74-75 -1.150842530998684 1101 76 -1.8739974015462515 1102 1 -0.460840849307953 1102 2 -1.5424290279008872 1102 3 -1.2983062696984256 1102 4 -0.3449942631303671 1102 5 -0.430912517511711 1102 6 0.6837114971633014 1102 7 0.7950668657507194 1102 8 1.0840650449721387 1102 9 -0.49756957953453806 1102 10-11 -1.0670760342532404 1102 12-13 -3.98077098147159 1102 14-15 -2.1084617883227423 1102 16-17 -3.9857578200221724 1102 18-19 -1.3837261369931042 1102 20-21 -1.0762456939835658 1102 22-23 -2.1914045010219567 1102 24-25 -0.9927011718993377 1102 26-27 -6.170240602859984 1102 28-29 -1.7206698685465334 1102 30-31 -1.3761820333668275 1102 32-33 -6.227905190594946 1102 34-35 -1.9544535872115816 1102 36-37 -0.6345613628879434 1102 38-39 -0.6678095814075355 1102 40-41 -1.0953759218737993 1102 42-43 -1.2534054062624875 1102 44-45 -5.327819070901267 1102 46-47 -0.8589031110019221 1102 48-49 -0.4076434876541981 1102 50-51 -5.90192100255495 1102 52-53 -2.305357048772205 1102 54-55 -1.140214461940026 1102 56-57 -2.422499063446349 1102 58-59 -0.6986004363183973 1102 60-61 -0.473091475315389 1102 62-63 -5.154528282029705 1102 64-65 -1.5687054476634898 1102 66-67 -0.7712361895835862 1102 68-69 -7.148025046752522 1102 70-71 -1.677307426035405 1102 72-73 -0.7463349634693763 1102 74-75 -6.317549865075652 1102 76 -1.9497782158239971 1103 1 -0.8083881040334262 1103 2 -1.8374335283509318 1103 3 -1.2066658556570218 1103 4 -0.9308403477388296 1103 5 -0.8884395202119819 1103 6 -2.709684092395655 1103 7 -0.5315532962654785 1103 8 -0.6291437759099487 1103 9 -1.1665614787244571 1103 10-11 -1.1244286445142677 1103 12-13 -2.219857390112452 1103 14-15 -2.533135505694478 1103 16-17 -2.036219116151784 1103 18-19 -1.9406568300624087 1103 20-21 -1.7842789973139013 1103 22-23 -1.7277737629481464 1103 24-25 -0.9375425310352519 1103 26-27 -2.202418820681764 1103 28-29 -1.474157717331412 1103 30-31 -1.1777684420076895 1103 32-33 -5.667289754051289 1103 34-35 -2.395469563809243 1103 36-37 -0.8960093826899254 1103 38-39 -1.2850181772671192 1103 40-41 -0.6763840226838767 1103 42-43 -0.26893195891774724 1103 44-45 -5.074499988993075 1103 46-47 -1.5790876294537668 1103 48-49 -1.4489069890043353 1103 50-51 -1.2444615066053544 1103 52-53 -1.1750127643437622 1103 54-55 -1.5618222477186023 1103 56-57 -0.9645220157415828 1103 58-59 -0.9905108773625031 1103 60-61 -0.7891918353513923 1103 62-63 -0.8761754467461778 1103 64-65 -0.5211694440631334 1103 66-67 -1.5221925352181493 1103 68-69 -3.355439538201665 1103 70-71 -0.9584730425970598 1103 72-73 -0.3864677267457033 1103 74-75 -2.6757013052196648 1103 76 -1.2205927972821442 1104 1 -2.036264777711157 1104 2 -0.1920993058095064 1104 3 -0.4840987802966339 1104 4 -0.49142619907007656 1104 5 -0.6485585372950382 1104 6 -1.521334428413713 1104 7 -0.2889533886063198 1104 8 0.7506335800639903 1104 9 -1.1877485715270026 1104 10-11 -0.834901634818479 1104 12-13 -0.6631190737937729 1104 14-15 -0.516757831657646 1104 16-17 -0.511222963686798 1104 18-19 -0.20108364994646877 1104 20-21 0.1295362184045601 1104 22-23 0.0963810618607539 1104 24-25 0.41611006220334446 1104 26-27 0.3647334904042623 1104 28-29 -0.39713006637978765 1104 30-31 -1.2894239080724361 1104 32-33 -1.2399312150885855 1104 34-35 -0.7106211566887382 1104 36-37 -0.30829840952007004 1104 38-39 -0.3948909521093711 1104 40-41 -0.1318808677051706 1104 42-43 -0.3684548645761936 1104 44-45 -3.983877714330376 1104 46-47 -0.9558767332346108 1104 48-49 -0.17352608303809092 1104 50-51 0.29541178124156175 1104 52-53 -0.309920167001124 1104 54-55 0.30312927334023954 1104 56-57 0.18315920315750844 1104 58-59 0.13650907851909366 1104 60-61 -0.940207584594706 1104 62-63 0.3342847184413209 1104 64-65 -0.31078622957587854 1104 66-67 -0.5052505560461391 1104 68-69 -2.864199092913612 1104 70-71 -0.8467021504819421 1104 72-73 -0.15739831721407782 1104 74-75 -1.7758782918302423 1104 76 -0.2363420406002561 1105 1 -1.3025699964494706 1105 2 0.10494679215534575 1105 3 -1.1553236080301943 1105 4 0.08202073218359729 1105 5 0.3173887945782603 1105 6 0.18482443062159604 1105 7 1.127423941664496 1105 8 1.999364951251426 1105 9 1.1732364185910455 1105 10-11 1.1324612197298904 1105 12-13 -0.6777016281445043 1105 14-15 0.36594074213649463 1105 16-17 0.7562173439890785 1105 18-19 1.162665615584217 1105 20-21 0.18418073525729284 1105 22-23 0.19633565830325495 1105 24-25 1.027829521633933 1105 26-27 0.7601717682740414 1105 28-29 0.11801335544596725 1105 30-31 0.7664491756303633 1105 32-33 1.5382323445503232 1105 34-35 0.8687950069777344 1105 36-37 1.3522298742254648 1105 38-39 0.7693096781988373 1105 40-41 1.0688696263923703 1105 42-43 1.2576067774301123 1105 44-45 1.5231064211324679 1105 46-47 1.094587985530417 1105 48-49 0.3576154158134628 1105 50-51 -0.22695614782205098 1105 52-53 0.28959374785384995 1105 54-55 -0.15289604582006433 1105 56-57 0.1818238044074434 1105 58-59 0.17364651775845275 1105 60-61 0.14856972430973414 1105 62-63 1.0272898997884745 1105 64-65 0.04545809497943054 1105 66-67 0.46558433525240517 1105 68-69 1.1517055061996864 1105 70-71 0.9865866695597241 1105 72-73 1.2384939962307193 1105 74-75 -0.5457890871937394 1105 76 -0.9487238578108759 1106 1 -0.6645934920140846 1106 2 0.13251811797649182 1106 3 -0.37032001177030693 1106 4 -0.7136517684157795 1106 5 -0.17338080503813913 1106 6 -0.1486087988197866 1106 7 0.6626672885837053 1106 8 1.0801009857755197 1106 9 0.3782232323047836 1106 10-11 -0.0415078524350605 1106 12-13 -0.00957647830033892 1106 14-15 0.43316083324173604 1106 16-17 0.5378943998509769 1106 18-19 -0.12209823001636266 1106 20-21 0.0939445808578725 1106 22-23 0.5805960275192703 1106 24-25 0.5606154242951646 1106 26-27 0.44894544365164535 1106 28-29 -0.15407367404336014 1106 30-31 -0.6913247394979045 1106 32-33 0.3255435409061178 1106 34-35 0.6554169201879958 1106 36-37 0.5958165440887981 1106 38-39 0.4164266765205795 1106 40-41 0.36313359186827654 1106 42-43 0.822968800925679 1106 44-45 1.3129050305786478 1106 46-47 1.031953125784547 1106 48-49 0.3999014383500281 1106 50-51 0.7409172638298287 1106 52-53 0.5568258264920658 1106 54-55 0.2557289841487673 1106 56-57 1.1304871944817698 1106 58-59 0.08826531421014394 1106 60-61 1.1314275521687556 1106 62-63 0.5912433881605672 1106 64-65 0.7395662225267827 1106 66-67 0.5431137047081656 1106 68-69 1.2532698792517074 1106 70-71 0.8229568445776998 1106 72-73 -0.03161509031927423 1106 74-75 -0.21996794118558682 1106 76 0.45112030426057004 1107 1 -0.05480716218289672 1107 2 0.22672187196067028 1107 3 0.2119902231488311 1107 4 -0.35439666276119297 1107 5 0.08532536896729326 1107 6 -0.5214396334319957 1107 7 -0.5574365952552789 1107 8 0.2634028391576493 1107 9 -1.060213788117835 1107 10-11 -0.8314824717243994 1107 12-13 0.5383222349566452 1107 14-15 -0.7072446678797348 1107 16-17 0.9458007863460836 1107 18-19 0.6733804619916697 1107 20-21 0.30163847768233154 1107 22-23 -0.12410235519823587 1107 24-25 -0.25549247323759516 1107 26-27 0.3158000062770654 1107 28-29 -0.5811336433504124 1107 30-31 0.0448781881375524 1107 32-33 0.39131204521493146 1107 34-35 0.05652068597715498 1107 36-37 -0.1302783679640882 1107 38-39 0.3711988403737294 1107 40-41 0.6436642257957885 1107 42-43 0.460600131347114 1107 44-45 0.24160294109688607 1107 46-47 0.014481752865172837 1107 48-49 0.6318840850269254 1107 50-51 0.7225310048285323 1107 52-53 0.7394341371991828 1107 54-55 0.09817674710473767 1107 56-57 0.7464268710252675 1107 58-59 0.30310122496448244 1107 60-61 -0.4204553424127546 1107 62-63 -0.31915680071913854 1107 64-65 0.43766857171814166 1107 66-67 0.8646371375968478 1107 68-69 0.8761154700910403 1107 70-71 -0.35872182382036044 1107 72-73 -0.25530473735031833 1107 74-75 -0.29847106950574087 1107 76 -2.4891760007801587 1108 1 -0.6037325320932538 1108 2 -0.7406882155217787 1108 3 -0.36128982869262316 1108 4 -0.47676408904913004 1108 5 0.25054299506082245 1108 6 0.010718266989222514 1108 7 0.7914401732942409 1108 8 0.6215408670224285 1108 9 -0.4438945311786391 1108 10-11 0.05423682841600197 1108 12-13 0.7552058076966901 1108 14-15 -0.2147238772975939 1108 16-17 -0.9043990192484763 1108 18-19 -0.16564103061012503 1108 20-21 0.3236575942175932 1108 22-23 -0.5815761644648951 1108 24-25 -0.4608467231565925 1108 26-27 -0.5718363475408346 1108 28-29 0.12314057632774222 1108 30-31 0.568571351546133 1108 32-33 -0.17513681535316294 1108 34-35 -0.37303434156361703 1108 36-37 -0.7961305118241135 1108 38-39 0.47864834895997177 1108 40-41 0.140842646791576 1108 42-43 0.2337802803912865 1108 44-45 -0.110338413261033 1108 46-47 0.13481062207351613 1108 48-49 -0.017713603708358505 1108 50-51 0.25922503226129834 1108 52-53 0.08620484677905438 1108 54-55 0.2145365051779642 1108 56-57 0.7645173775594536 1108 58-59 -1.2652106877690734 1108 60-61 0.21344140669621936 1108 62-63 -0.4886675470200359 1108 64-65 -0.02385535095169189 1108 66-67 -0.06487738880989014 1108 68-69 0.5218250499592791 1108 70-71 0.8592012780264859 1108 72-73 0.5473062357569276 1108 74-75 0.497065705523962 1108 76 0.6998832928800667 1109 1 0.011049102845156256 1109 2 0.7032647520034168 1109 3 0.1403899025503783 1109 4 0.6941205694055164 1109 5 0.6925922671772824 1109 6 0.7366421191728705 1109 7 -0.6749092108043016 1109 8 0.672940643058265 1109 9 1.3696553008482368 1109 10-11 1.9282289896702025 1109 12-13 0.7447074874279309 1109 14-15 0.9872918001940079 1109 16-17 0.5643019885902731 1109 18-19 1.2899580735332137 1109 20-21 1.3200461720451386 1109 22-23 1.5086254032842632 1109 24-25 0.8686325601580833 1109 26-27 1.4481804497715913 1109 28-29 0.8512200836065773 1109 30-31 1.00521748816427 1109 32-33 1.7869572495964476 1109 34-35 0.7461145946065955 1109 36-37 1.1789254792173196 1109 38-39 0.5495050118938991 1109 40-41 0.6742413030065819 1109 42-43 1.2032931583308155 1109 44-45 0.902623512830786 1109 46-47 0.9158576545483186 1109 48-49 0.4073983783745021 1109 50-51 1.584980911320649 1109 52-53 1.035252999074686 1109 54-55 1.1421120930838953 1109 56-57 0.7135655298550851 1109 58-59 0.6701196593753842 1109 60-61 1.214169290234853 1109 62-63 0.8430793734726869 1109 64-65 0.7848232604704819 1109 66-67 0.8867183558709577 1109 68-69 1.8239247140130281 1109 70-71 0.8692236744430595 1109 72-73 0.7667631226550249 1109 74-75 2.153532670809515 1109 76 -0.05946721103930841 1110 1 -0.27090578437288926 1110 2 0.14317703270517157 1110 3 -0.6957629796551359 1110 4 -0.6653405834766879 1110 5 -0.9154904395896324 1110 6 -0.972944346992545 1110 7 -1.5772275065436503 1110 8 0.2545195904266855 1110 9 -2.4304700124349736 1110 10-11 -1.5861507095779181 1110 12-13 -1.3016584273590368 1110 14-15 -0.5529964203573741 1110 16-17 -0.7855413698057205 1110 18-19 0.1817500534830856 1110 20-21 -1.1831179883558605 1110 22-23 -0.8398081806756359 1110 24-25 -0.6424576654058285 1110 26-27 -1.229764412383794 1110 28-29 -1.155734803611466 1110 30-31 -0.9232599554447489 1110 32-33 -0.4428673118070634 1110 34-35 -0.1848377863457813 1110 36-37 -0.8443827914593705 1110 38-39 -0.5131829580309102 1110 40-41 -0.7279516794495535 1110 42-43 -0.34999631535339404 1110 44-45 0.27283027974808505 1110 46-47 -0.6925070071058173 1110 48-49 -0.6112419725026825 1110 50-51 -0.41301407614802343 1110 52-53 -1.2958873518025094 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0.8440254237770404 2116 44-45 2.014522826331941 2116 46-47 1.7342787071798966 2116 48-49 1.7287942593813668 2116 50-51 0.7125552820300314 2116 52-53 2.082735836603291 2116 54-55 0.5559564866765783 2116 56-57 1.1371353239054294 2116 58-59 1.1150853344325888 2116 60-61 -0.13480096148139253 2116 62-63 1.455550769353696 2116 64-65 1.691917081980776 2116 66-67 1.2280227036970466 2116 68-69 1.486510526370008 2116 70-71 -0.25823627979034924 2116 72-73 1.7587539693369472 2116 74-75 -0.09074650539185569 2116 76 1.5876723770613772 2117 1 0.9290942156271136 2117 2 0.6729389374670767 2117 3 0.3173322584401035 2117 4 0.014421321285215782 2117 5 -1.0357285348277294 2117 6 -0.7169919660401618 2117 7 -2.616513220829365 2117 8 -1.2716708857637897 2117 9 0.024291892326935738 2117 10-11 0.0477778618506548 2117 12-13 0.6983415726409632 2117 14-15 -0.044663087024041204 2117 16-17 0.06743482067047069 2117 18-19 0.5853214820545105 2117 20-21 -0.5741894169272896 2117 22-23 0.6970965812291254 2117 24-25 0.9992090012608372 2117 26-27 0.9749974923781153 2117 28-29 1.1692651963885368 2117 30-31 0.7451924255076321 2117 32-33 1.5095136405738856 2117 34-35 0.46754316603517054 2117 36-37 0.07704577996920392 2117 38-39 0.6981265657786153 2117 40-41 0.07085784435996345 2117 42-43 0.18274177988470086 2117 44-45 1.085330279748085 2117 46-47 1.2580882309894221 2117 48-49 0.7822104084496928 2117 50-51 1.8131764000424582 2117 52-53 1.1910174101022548 2117 54-55 -0.3705445485201082 2117 56-57 -0.6520986305459182 2117 58-59 0.3554580052400418 2117 60-61 1.2683046285807116 2117 62-63 -0.5274720049734292 2117 64-65 -0.10891107536912159 2117 66-67 -0.3204245012718978 2117 68-69 0.5498645636370902 2117 70-71 0.8589479852200093 2117 72-73 0.13142477679036446 2117 74-75 0.1852369314611444 2117 76 -0.07650981755559982 2118 1 1.6209111365702462 2118 2 1.5707660294689276 2118 3 1.3953720180332603 2118 4 1.70873477482197 2118 5 1.7258063703964979 2118 6 2.8035813118146677 2118 7 1.4785399460684587 2118 8 -2.199363904718947 2118 9 0.9404268900153312 2118 10-11 1.4354338951377557 2118 12-13 2.308836255951185 2118 14-15 0.9306489795501633 2118 16-17 1.2654006089275214 2118 18-19 1.49734182417194 2118 20-21 -0.24030129857315075 2118 22-23 1.405048499860655 2118 24-25 0.9052191723195548 2118 26-27 1.7593710476254572 2118 28-29 1.0067131852928313 2118 30-31 2.001410640949146 2118 32-33 1.4179741121411595 2118 34-35 1.2300951771308704 2118 36-37 1.0477808701217626 2118 38-39 1.8435264733144479 2118 40-41 0.1826470260520665 2118 42-43 1.4176007720252457 2118 44-45 1.681724177112855 2118 46-47 0.8439874450439717 2118 48-49 -0.1563471504962095 2118 50-51 0.5822471351326044 2118 52-53 0.17053659641755559 2118 54-55 1.7468387154650955 2118 56-57 1.9046743699163962 2118 58-59 2.287774232701903 2118 60-61 0.2583184982062363 2118 62-63 2.2044743658078936 2118 64-65 1.5918748700770209 2118 66-67 0.5551649578127105 2118 68-69 1.828089251570514 2118 70-71 0.8695813647854287 2118 72-73 0.4515357337945254 2118 74-75 1.3662817765836657 2118 76 1.241197071025077 2119 1 0.41546531579131596 2119 2 1.3199011706362072 2119 3 1.470698432495933 2119 4 1.067623291728566 2119 5 1.1820054553200556 2119 6 1.4160129600682154 2119 7 0.13192684485207806 2119 8 2.418312693874963 2119 9 2.0042590516044356 2119 10-11 1.5806185843465528 2119 12-13 0.8986699798659217 2119 14-15 0.14055858785280861 2119 16-17 1.7744955760070837 2119 18-19 0.2628255871448246 2119 20-21 0.5236759361104717 2119 22-23 1.7921704728547425 2119 24-25 1.6299150767944965 2119 26-27 0.5625180178296674 2119 28-29 2.1326477908056063 2119 30-31 0.6189198475109166 2119 32-33 1.591615446813627 2119 34-35 1.6386433302387786 2119 36-37 0.5353380623994113 2119 38-39 1.5749738564190068 2119 40-41 0.928657515952743 2119 42-43 0.7139404662558064 2119 44-45 -0.6002198023537204 2119 46-47 0.8917499715477177 2119 48-49 0.9713729700260458 2119 50-51 1.3583323934743063 2119 52-53 0.20842299302507428 2119 54-55 0.26525183900041327 2119 56-57 2.1654711559893833 2119 58-59 3.122781486356626 2119 60-61 2.466498388843444 2119 62-63 1.9324623135815848 2119 64-65 1.4500380215110127 2119 66-67 1.8539597351813093 2119 68-69 0.9626724454104867 2119 70-71 1.0699496272561326 2119 72-73 0.13421623820251583 2119 74-75 1.5280940743182931 2119 76 1.5615854205396396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 14.0 13 85.0 14 356.0 15 869.0 16 1381.0 17 1665.0 18 1797.0 19 1704.0 20 1398.0 21 1099.0 22 814.0 23 641.0 24 587.0 25 621.0 26 606.0 27 658.0 28 841.0 29 1007.0 30 1222.0 31 1556.0 32 2182.0 33 2854.0 34 4156.0 35 6458.0 36 11180.0 37 12169.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.471998888638055 20.270199871498775 9.620226787295744 32.63757445256742 2 17.513207416870966 21.439176824004697 32.28134387624736 28.76627188287697 3 15.76430371879424 23.933911121853527 28.24142812748179 32.060357031870446 4 11.874589965816098 24.432857981423293 34.33065156589897 29.361900486861643 5 11.16501502019958 39.81216118227962 32.02237491799316 17.000448879527642 6 22.402541348710333 36.25047477642347 17.15065087531508 24.19633299955112 7 22.17292220572494 30.396049860156765 20.57249404371396 26.858533890404335 8 19.942336245295397 34.396257035323366 21.461620800386726 24.19978591899451 9 21.358033217085044 20.23756085770519 20.92987120610476 37.474534719105 10-11 21.295880667104036 26.349228272504405 27.07434135561617 25.28054970477539 12-13 20.875487724871377 24.09619833569283 26.30865646904458 28.719657470391212 14-15 20.495666586098547 25.737198301163634 23.48071544490867 30.28641966782915 16-17 22.087462449501054 27.760609094989814 24.504506059873624 25.64742239563551 18-19 20.520700252063122 26.853354511239253 25.049204102068302 27.57674113462933 20-21 19.608266289147476 27.365249818721733 25.45837505610994 27.568108836020855 22-23 22.39045613065847 26.84126929318739 25.189047339525565 25.579227236628572 24-25 20.7390974068575 29.037326059183037 24.796277752840027 25.427298781119433 26-27 19.414039570456822 29.22896308829115 26.564172507855393 24.792824833396637 28-29 20.92814474638307 27.71226822278236 27.792548599841165 23.567038430993406 30-31 20.802976416560202 27.259935775698352 27.770104623459137 24.166983184282312 32-33 19.01091122544111 29.809916784641416 26.48302890093574 24.696143088981735 34-35 20.36790856669314 28.282863160802457 26.74286108905079 24.60636718345361 36-37 20.82887331238562 27.62249231725424 26.641863195331656 24.906771175028485 38-39 20.105486688995548 26.981975760505506 28.43910776561583 24.47342978488312 40-41 19.968233141120816 26.50374641759608 27.890956803977762 25.637063637305342 42-43 18.872794447705534 26.4804392113532 28.688581195400708 25.95818514554055 44-45 18.671661890128103 27.296191429853938 28.7248368495563 25.307309830461655 46-47 18.939263146990783 27.714857912364906 28.19481371499603 25.151065225648285 48-49 18.78749687076474 28.415182617853475 27.10737808931053 25.689942422071248 50-51 18.606919650564553 27.448983115223925 26.886157245951452 27.05793998826007 52-53 18.47484548185491 27.38424087566037 27.493871067987985 26.647042574496737 54-55 18.11056248057733 27.773557542902523 26.824867925831292 27.291012050688856 56-57 18.726045371361487 27.850385000517935 26.332826905148305 27.090742722972273 58-59 18.9651600428162 27.488691688822897 26.53827561202997 27.007872656330928 60-61 18.401470943682884 26.58489002451573 26.784296122371465 28.22934290942992 62-63 18.635406235972514 27.034632782017194 26.572804806463868 27.757156175546427 64-65 19.187873346914817 27.847795310935396 25.271917406166917 27.692413935982874 66-67 18.97120265184213 28.631608024584786 24.8705155208729 27.526673802700184 68-69 18.60174027139947 28.591899450985807 25.03193950485135 27.77442077276337 70-71 19.710990642588307 29.200476502883188 24.439763820310073 26.648769034218432 72-73 19.15075446289838 28.388177203825837 23.67925831290356 28.781810020372223 74-75 19.059252097648564 28.30789682676703 24.32409101895653 28.30876005662788 76 19.325990124650392 26.94485687648907 24.6262214702531 29.10293152860744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 1.5 16 3.0 17 4.0 18 3.5 19 8.0 20 14.5 21 16.0 22 22.0 23 35.0 24 44.5 25 47.0 26 89.0 27 162.0 28 230.5 29 268.0 30 295.0 31 416.5 32 683.5 33 856.0 34 1028.0 35 1287.0 36 1480.0 37 1586.0 38 1902.0 39 2457.5 40 2920.0 41 3403.0 42 3663.0 43 3723.0 44 4019.0 45 4233.0 46 4211.0 47 4237.5 48 4090.0 49 3763.0 50 3610.0 51 3368.0 52 2794.0 53 2314.5 54 2167.0 55 2079.5 56 1731.0 57 1314.5 58 1159.0 59 1015.5 60 731.5 61 527.0 62 463.0 63 400.0 64 281.5 65 199.5 66 151.5 67 130.0 68 113.5 69 76.0 70 52.0 71 49.0 72 47.5 73 37.5 74 21.5 75 14.0 76 19.0 77 19.0 78 11.5 79 9.0 80 7.5 81 6.0 82 5.0 83 4.0 84 3.0 85 2.0 86 3.5 87 5.0 88 5.5 89 5.5 90 3.0 91 1.5 92 2.0 93 2.5 94 1.5 95 0.0 96 0.0 97 1.5 98 4.0 99 6.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5783640067677221 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 8.632298608473465E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 57922.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.1143952211595 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20594741986932 54.50088049445806 2 8.692185007974482 11.667414799212734 3 3.9898132427843804 8.033217085045406 4 1.9859031743581828 5.331307620593211 5 1.283634305705613 4.307517005628259 6 0.8488964346349746 3.418390248955492 7 0.6276688789422236 2.9487932046545358 8 0.38586201574317025 2.0717516660336317 9 0.3138344394711118 1.8956527744207727 >10 0.6662550805165406 5.825075100997894 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0034529194433893856 0.0 2 0.0 0.0 0.0 0.005179379165084079 0.0 3 0.0 0.0 0.0 0.005179379165084079 0.0 4 0.0 0.0 0.0 0.005179379165084079 0.0 5 0.0 0.0 0.0 0.005179379165084079 0.0 6 0.0 0.0 0.0 0.005179379165084079 0.0 7 0.0 0.0 0.0 0.005179379165084079 0.0 8 0.0 0.0 0.0 0.005179379165084079 0.0 9 0.0 0.0 0.0 0.005179379165084079 0.0 10 0.0 0.0 0.0 0.006905838886778771 0.0 11 0.0 0.0 0.0 0.006905838886778771 0.0 12 0.0 0.0 0.0 0.006905838886778771 0.0 13 0.0 0.0 0.0 0.006905838886778771 0.0 14 0.0 0.0 0.0 0.006905838886778771 0.0 15 0.0 0.0 0.0 0.006905838886778771 0.0 16 0.0 0.0 0.0 0.008632298608473464 0.0 17 0.0 0.0 0.0 0.008632298608473464 0.0 18 0.0 0.0 0.0 0.013811677773557542 0.0 19 0.0 0.0 0.0 0.013811677773557542 0.0 20 0.0 0.0 0.0 0.013811677773557542 0.0 21 0.0 0.0 0.0 0.013811677773557542 0.0 22 0.0 0.0 0.0 0.018991056938641622 0.0 23 0.0 0.0 0.0 0.018991056938641622 0.0 24 0.0 0.0 0.0 0.020717516660336315 0.0 25 0.0 0.0 0.0 0.02244397638203101 0.0 26 0.0 0.0 0.0 0.0241704361037257 0.0 27 0.0 0.0 0.0 0.027623355547115085 0.0 28 0.0 0.0 0.0 0.03280273471219917 0.0 29 0.0 0.0 0.0 0.04143503332067263 0.0 30 0.0 0.0 0.0 0.05006733192914609 0.0 31 0.0 0.0 0.0 0.06733192914609303 0.0 32 0.0 0.0 0.0 0.08804944580642933 0.0 33 0.0 0.0 0.0 0.10876696246676565 0.0 34 0.0 0.0 0.0 0.13121093884879667 0.0 35 0.0 0.0 0.0 0.15192845550913298 0.0 36 0.0 0.0 0.0 0.20199578743827906 0.0 37 0.0 0.0 0.0 0.3470184040606333 0.0 38 0.0 0.0 0.0 0.5472877317772177 0.0 39 0.0 0.0 0.0 0.7510099789371913 0.0 40 0.0 0.0 0.0 0.9996201788612271 0.0 41 0.0 0.0 0.0 1.2862124926625462 0.0 42 0.0 0.0 0.0 1.612513380062843 0.0 43 0.0 0.0 0.0 2.0216843341044854 0.0 44 0.0 0.0 0.0 2.360070439556645 0.0 45 0.0 0.0 0.0 2.732985739442699 0.0 46 0.0 0.0 0.0 3.0920893615551948 0.0 47 0.0 0.0 0.0 3.4926280169883634 0.0 48 0.0 0.0 0.0 3.865543316874417 0.0 49 0.0 0.0 0.0 4.195297123718103 0.0 50 0.0 0.0 0.0 4.587203480542799 0.0 51 0.0 0.0 0.0 5.048168226235282 0.0 52 0.0 0.0 0.0 5.457339180276924 0.0 53 0.0 0.0 0.0 5.842339698214841 0.0 54 0.0 0.0 0.0 6.206622699492421 0.0 55 0.0 0.0 0.0 6.569179241048307 0.0 56 0.0 0.0 0.0 6.990435413141811 0.0 57 0.0 0.0 0.0 7.320189219985497 0.0 58 0.0 0.0 0.0 7.668934083767826 0.0 59 0.0 0.0 0.0 8.034943544767101 0.0 60 0.0 0.0 0.0 8.40267946548807 0.0 61 0.0 0.0 0.0 8.753150788992093 0.0 62 0.0 0.0 0.0 9.207209695797797 0.0 63 0.0 0.0 0.0 9.626739408169607 0.0 64 0.0 0.0 0.0 10.008287006664135 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCGGA 20 7.868185E-5 69.99482 25 GGGTCGG 20 7.868185E-5 69.99482 24 GTCGGAG 20 7.868185E-5 69.99482 26 CTCCGGA 15 0.0022148704 69.99481 12 TTGCTAA 15 0.0022148704 69.99481 27 CCGGATC 15 0.0022148704 69.99481 14 AGGTTGT 15 0.0022148704 69.99481 57 CGGATCC 15 0.0022148704 69.99481 15 GGGAACA 15 0.0022148704 69.99481 43 AAGGTTG 30 8.284076E-6 58.329014 36 AGGTGTT 25 2.3729254E-4 55.995857 43 ATTTATG 20 0.0069209333 52.496117 3 GGATCCA 20 0.0069209333 52.496117 16 TTGGGGA 20 0.0069209333 52.496117 40 TCTGCTT 20 0.0069209333 52.496117 60 GGGAGGG 30 5.835143E-4 46.663208 41 TTGATTT 35 0.0012463733 39.99704 66 AGGAAAA 40 0.002401444 34.99741 31 GGAAAAG 40 0.002401444 34.99741 32 AGGTTGG 55 2.960016E-4 31.815828 37 >>END_MODULE