FastQCFastQC Report
Wed 25 May 2016
SRR1780386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57922
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT29165.034356548461724No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC12472.152895272953282No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT10091.7419978591899452No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC3580.6180725803667001No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT2020.34874486378232794No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1280.2209868443769207No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT1240.21408100549014192No Hit
CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC1220.2106280860467525No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT1000.17264597216946928No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT920.15883429439591173No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG790.13639031801388074No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC710.12257864024032321No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG680.11739926107523911No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT680.11739926107523911No Hit
CTTATCACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC590.10186112357998688No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCT150.002214220470.050
GTCTGCC207.865301E-570.039
GCACACG150.002214220470.015
CTGCCGT207.865301E-570.041
GCGGCTG150.002214220470.010
CTTTATA207.865301E-570.02
CTTTACA2000.059.5000041
TTTACAC2150.056.9767462
TCTGCCG252.372057E-456.00000440
CGTCTGC252.372057E-456.00000438
TTACACA2200.054.0909083
TTGGCAG200.006918907652.539
GGCAAAA200.006918907652.565
AGCGGCT200.006918907652.59
TACGAGT200.006918907652.54
TCTCCTG200.006918907652.550
CGAGCAC200.006918907652.512
GGGCAAA200.006918907652.564
TGGGCAA200.006918907652.563
CTCTCGT200.006918907652.534
Produced by FastQC (version 0.11.5)