##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57922 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23754359310797 34.0 34.0 34.0 27.0 34.0 2 32.99502779600152 34.0 34.0 34.0 32.0 34.0 3 33.290407789786265 34.0 34.0 34.0 33.0 34.0 4 33.3707226960395 34.0 34.0 34.0 33.0 34.0 5 33.39850143296157 34.0 34.0 34.0 34.0 34.0 6 37.067090224785055 38.0 37.0 38.0 36.0 38.0 7 36.69027312592797 38.0 37.0 38.0 34.0 38.0 8 36.63316183833431 38.0 37.0 38.0 34.0 38.0 9 36.40632229550084 38.0 37.0 38.0 34.0 38.0 10-11 36.06232519595318 38.0 36.5 38.0 32.5 38.0 12-13 35.9555868236594 38.0 36.5 38.0 32.0 38.0 14-15 36.08952556886848 38.0 37.0 38.0 33.0 38.0 16-17 36.26392389765547 38.0 37.0 38.0 33.5 38.0 18-19 36.314431476813645 38.0 37.0 38.0 34.0 38.0 20-21 36.036730430579055 38.0 37.0 38.0 33.0 38.0 22-23 36.007691378060144 38.0 36.5 38.0 32.5 38.0 24-25 35.12154276440731 37.5 35.0 38.0 29.0 38.0 26-27 35.406028797348156 37.5 36.0 38.0 31.0 38.0 28-29 35.1217413072753 37.5 35.5 38.0 29.0 38.0 30-31 34.774524360346675 37.0 34.5 38.0 27.0 38.0 32-33 35.25751873208798 37.5 36.0 38.0 30.5 38.0 34-35 35.00949552846932 37.5 35.5 38.0 27.0 38.0 36-37 35.13494009184765 38.0 36.0 38.0 28.5 38.0 38-39 34.867373364179414 37.5 35.0 38.0 26.5 38.0 40-41 34.59031974034046 37.0 34.5 38.0 26.0 38.0 42-43 35.20196989054245 37.0 35.5 38.0 29.0 38.0 44-45 35.46227685508097 37.5 36.0 38.0 31.0 38.0 46-47 35.69938020095991 38.0 36.0 38.0 31.0 38.0 48-49 35.35444218086392 37.5 36.0 38.0 29.0 38.0 50-51 35.57169123994337 38.0 36.0 38.0 31.0 38.0 52-53 35.462691205414174 38.0 36.0 38.0 31.0 38.0 54-55 35.601075584406615 38.0 36.0 38.0 31.0 38.0 56-57 35.644660060080795 38.0 36.0 38.0 31.5 38.0 58-59 35.75470460274162 38.0 36.0 38.0 31.5 38.0 60-61 35.4043454991195 38.0 36.0 38.0 29.0 38.0 62-63 35.77789958910259 38.0 36.0 38.0 31.5 38.0 64-65 35.76897379234143 38.0 36.5 38.0 31.5 38.0 66-67 34.798979662304475 37.5 34.5 38.0 26.0 38.0 68-69 33.33144573737095 37.0 33.5 38.0 22.0 38.0 70-71 32.563024412140464 37.0 31.5 38.0 16.0 38.0 72-73 31.96409827008736 37.0 29.0 38.0 11.0 38.0 74-75 32.49852387693795 37.0 32.0 38.0 11.0 38.0 76 32.11658782500604 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9870251823493206 1101 2 0.6841475402605184 1101 3 0.06580800058505076 1101 4 0.5122802465306506 1101 5 0.39561921211523554 1101 6 -1.475468158929047 1101 7 0.25803985305212507 1101 8 0.779825032052031 1101 9 0.3287436249418434 1101 10-11 0.4417682467995476 1101 12-13 1.1235972046476164 1101 14-15 1.0921750596063475 1101 16-17 0.31850472060506263 1101 18-19 -0.5806420615127124 1101 20-21 0.23505886105102292 1101 22-23 0.8542885211939861 1101 24-25 -1.1389030029131035 1101 26-27 -0.20638175221888133 1101 28-29 0.6772266283817459 1101 30-31 0.793180904351793 1101 32-33 -0.2693127792005683 1101 34-35 0.4079377316300494 1101 36-37 0.5472785024034437 1101 38-39 -0.6395111474404302 1101 40-41 1.305745904646784 1101 42-43 0.5625142975003925 1101 44-45 0.5753996271584327 1101 46-47 0.5602142621728206 1101 48-49 -1.4857354233885403 1101 50-51 -0.9281297938731115 1101 52-53 -1.527672748152149 1101 54-55 -0.5584511292443182 1101 56-57 -0.9730503700968782 1101 58-59 -1.0701826506025824 1101 60-61 -1.0917206179634462 1101 62-63 -0.6014774335720645 1101 64-65 -1.5250648256454085 1101 66-67 -1.337751520508668 1101 68-69 -0.43937609744892114 1101 70-71 -0.4199489363254969 1101 72-73 -2.1164423700044175 1101 74-75 -1.2720873728209057 1101 76 -0.6038698921852301 1102 1 -0.2602210530104081 1102 2 0.3808848520285082 1102 3 0.13883505022252507 1102 4 0.6063265376628308 1102 5 0.0694201044744105 1102 6 0.2512468438595761 1102 7 -0.20382573534720905 1102 8 0.5599072964134351 1102 9 0.6963886333077127 1102 10-11 0.575395547413045 1102 12-13 0.7370941371629556 1102 14-15 -0.06607926275840015 1102 16-17 0.43611487119067505 1102 18-19 -0.6185498975417048 1102 20-21 0.4931632949606737 1102 22-23 0.7227701162860072 1102 24-25 -5.490042852327491 1102 26-27 -1.5161279875786065 1102 28-29 0.05501525080227054 1102 30-31 1.8034290917472262 1102 32-33 0.4476071873359615 1102 34-35 1.1083873969953544 1102 36-37 -2.8782652789685557 1102 38-39 -0.17027313851684056 1102 40-41 0.9242449286289371 1102 42-43 1.1161778502053536 1102 44-45 1.0269647414919447 1102 46-47 1.3132120312748796 1102 48-49 -4.334748948207277 1102 50-51 -0.9213150978329523 1102 52-53 -0.18441075707573873 1102 54-55 0.4163118378499462 1102 56-57 0.888912820087171 1102 58-59 0.37904699245375184 1102 60-61 -6.204816686005827 1102 62-63 -2.7570128490768084 1102 64-65 -0.027313152471954538 1102 66-67 1.3121020768142557 1102 68-69 -1.636461987019473 1102 70-71 -2.232762656347809 1102 72-73 -5.610638549591702 1102 74-75 -3.174398443651917 1102 76 -1.0521758040647633 1103 1 1.3209558146763598 1103 2 0.8221539789411949 1103 3 0.22698761547905377 1103 4 0.5749358985989232 1103 5 0.5380294654105029 1103 6 0.4884205612313153 1103 7 0.9963317804043683 1103 8 0.8386539210759025 1103 9 0.5075384982942097 1103 10-11 0.39748713157145943 1103 12-13 0.026698097558991662 1103 14-15 -0.036826337465868164 1103 16-17 0.2744362033238872 1103 18-19 0.9267077532233685 1103 20-21 -0.24623251962078996 1103 22-23 0.43130972024640357 1103 24-25 -3.4973279558378394 1103 26-27 -0.45660953573342766 1103 28-29 0.018674116688856657 1103 30-31 1.183829631801224 1103 32-33 1.1046166382810583 1103 34-35 -0.15969878662300374 1103 36-37 -0.9264200944501084 1103 38-39 -0.28179991619460765 1103 40-41 -0.6345140722711591 1103 42-43 -0.46553344592425816 1103 44-45 1.322559926010399 1103 46-47 1.2932975398257298 1103 48-49 -2.878206418954349 1103 50-51 -0.06873296461963463 1103 52-53 0.5675863176317293 1103 54-55 -0.027882581592002964 1103 56-57 0.5349524240475674 1103 58-59 -0.36378041528702454 1103 60-61 -3.86753608294552 1103 62-63 -1.3100056483567286 1103 64-65 0.4888603398772773 1103 66-67 -0.6515005334467716 1103 68-69 -0.13274911573233794 1103 70-71 0.0024983697548037753 1103 72-73 -4.237410601796924 1103 74-75 -2.444959874795032 1103 76 -3.7261744539297474 1104 1 0.6476628377102713 1104 2 0.40785140878780624 1104 3 0.44627146096204484 1104 4 -0.20849918066029716 1104 5 -0.8360791889782533 1104 6 0.21686154004422065 1104 7 0.5495303645114831 1104 8 0.7442867123343007 1104 9 1.0948518928461013 1104 10-11 -0.5338984940377358 1104 12-13 0.4391666760230635 1104 14-15 0.5459084904210627 1104 16-17 0.7094898123117304 1104 18-19 1.1102095230894733 1104 20-21 0.6221817829493617 1104 22-23 0.4756846240580259 1104 24-25 1.205449964441236 1104 26-27 1.5267947632456966 1104 28-29 1.8166144029454685 1104 30-31 0.9032289033346004 1104 32-33 0.7983785015140157 1104 34-35 0.22992825898310087 1104 36-37 1.2974294925811307 1104 38-39 0.8401925939372319 1104 40-41 1.5447554090811266 1104 42-43 0.7019497531728547 1104 44-45 0.9453732436115843 1104 46-47 1.3182167415904686 1104 48-49 0.42765978942814087 1104 50-51 0.7322853496470287 1104 52-53 0.5832970855997175 1104 54-55 0.42231749022865017 1104 56-57 0.25419779418043476 1104 58-59 -0.7168815285071588 1104 60-61 0.8055901675033539 1104 62-63 0.19994664220910607 1104 64-65 0.6395939365530054 1104 66-67 -0.07699943048672964 1104 68-69 2.4043411217888178 1104 70-71 2.1573103535438563 1104 72-73 1.4659187985006241 1104 74-75 -0.023064835550172802 1104 76 -0.31174363260927507 1105 1 1.4632442703260509 1105 2 0.653377822975088 1105 3 0.5251401111409919 1105 4 -0.01820485877578193 1105 5 -0.15304943629409706 1105 6 -0.04725362474398764 1105 7 0.8068349956743504 1105 8 -0.5305917663794375 1105 9 0.4587480522392937 1105 10-11 0.3785527639078907 1105 12-13 -0.2448646257236362 1105 14-15 0.40861614492669673 1105 16-17 0.45239884494887406 1105 18-19 1.002496259909087 1105 20-21 0.5523537823083728 1105 22-23 0.07392991010043914 1105 24-25 0.7836509152451399 1105 26-27 0.22344558318052776 1105 28-29 1.121205503848195 1105 30-31 1.5697130655054181 1105 32-33 1.1900509549085925 1105 34-35 -1.1917238963504744 1105 36-37 0.9579577763266585 1105 38-39 0.16170319700330538 1105 40-41 0.3301434759578967 1105 42-43 0.23669682864959185 1105 44-45 0.7327109926634563 1105 46-47 1.1999271203095532 1105 48-49 0.39026862442627674 1105 50-51 1.177413815006787 1105 52-53 0.12987579400205362 1105 54-55 1.0695452024235124 1105 56-57 0.5152014798809859 1105 58-59 0.7034085201013625 1105 60-61 0.7577828922509653 1105 62-63 -0.5343090065276002 1105 64-65 0.9552020771437881 1105 66-67 0.62858520708604 1105 68-69 0.6277132707124835 1105 70-71 1.488769677297924 1105 72-73 2.003964308890019 1105 74-75 0.030644902177506594 1105 76 1.1328640272379573 1106 1 -0.6353531883169623 1106 2 0.6504408773984878 1106 3 0.46771982823521085 1106 4 0.388831823075094 1106 5 -0.008539726392399416 1106 6 -0.06799205677467057 1106 7 -1.3008130503577817 1106 8 0.12135021396100854 1106 9 -0.9837117895252732 1106 10-11 0.8101339195060646 1106 12-13 1.5277918115815652 1106 14-15 -0.12369025641591236 1106 16-17 0.40942353926678265 1106 18-19 0.7399992567923306 1106 20-21 0.1889217516202919 1106 22-23 0.5149212790767095 1106 24-25 -0.14391441680951544 1106 26-27 1.063681737579948 1106 28-29 1.2764740245866264 1106 30-31 0.924993573776824 1106 32-33 0.32934608797021525 1106 34-35 1.3392938451983127 1106 36-37 1.7189863066551183 1106 38-39 0.5882495887558861 1106 40-41 1.4013194529418342 1106 42-43 1.4225335584506027 1106 44-45 1.572617489906321 1106 46-47 0.8671232363488741 1106 48-49 1.7115644767398663 1106 50-51 0.1976727457188474 1106 52-53 0.8782874458840908 1106 54-55 0.4060052839387396 1106 56-57 0.8307732852034491 1106 58-59 0.9589227852655853 1106 60-61 1.405978768539626 1106 62-63 1.1351647407752026 1106 64-65 -1.0394035330216411 1106 66-67 0.7250249097952306 1106 68-69 1.4565612687944807 1106 70-71 0.4546052083456402 1106 72-73 -0.07476381386231878 1106 74-75 -0.4812826931233758 1106 76 -0.14506692457216275 1107 1 1.1902577194861266 1107 2 -0.5488567261902304 1107 3 -0.04508857691175905 1107 4 0.12484291975789574 1107 5 0.09301262870145166 1107 6 0.349077946766819 1107 7 0.9133293177215478 1107 8 0.15897859313701446 1107 9 -0.045333794011163775 1107 10-11 0.2659095068039079 1107 12-13 0.0035915990918908847 1107 14-15 0.0507873731437698 1107 16-17 0.5800350373189644 1107 18-19 0.41942022243983246 1107 20-21 0.9334176789015984 1107 22-23 0.14440717212357157 1107 24-25 0.18100294831856445 1107 26-27 -0.6630476922394308 1107 28-29 0.9656636125927633 1107 30-31 0.29760306498210554 1107 32-33 1.2422253230073963 1107 34-35 0.6806791598010804 1107 36-37 1.457683036675185 1107 38-39 -0.10147122342685577 1107 40-41 0.7272992664231168 1107 42-43 1.118009299213199 1107 44-45 0.9147848845468616 1107 46-47 0.918253563346866 1107 48-49 0.6915402765273768 1107 50-51 1.3765477309626135 1107 52-53 0.8936781907784379 1107 54-55 0.918503808316018 1107 56-57 0.8356133277013811 1107 58-59 0.6848701350471984 1107 60-61 1.59696861625536 1107 62-63 0.9827771832259131 1107 64-65 1.1239183888293809 1107 66-67 -0.2443411626873555 1107 68-69 1.3629034952140273 1107 70-71 0.0847846440675113 1107 72-73 0.9787275094761299 1107 74-75 -0.8800254625720854 1107 76 0.2517870478041786 1108 1 -0.1401775327009318 1108 2 0.6410395909066509 1108 3 0.214874702060051 1108 4 0.4905141585525783 1108 5 0.08126729983224124 1108 6 -0.2661612799315236 1108 7 0.06769263989457386 1108 8 -0.21511688153627517 1108 9 -0.08588892955496163 1108 10-11 0.3194115048598505 1108 12-13 1.3727324350352959 1108 14-15 0.5947041028122015 1108 16-17 0.06256554817326787 1108 18-19 0.3468151894989049 1108 20-21 1.2351241266821376 1108 22-23 0.1975041588392017 1108 24-25 1.0173918672082962 1108 26-27 -0.5091163821627518 1108 28-29 -0.8427120219250028 1108 30-31 0.37136913043193687 1108 32-33 1.4211323324036655 1108 34-35 1.0677079966085046 1108 36-37 1.628746326447299 1108 38-39 1.2425049079048236 1108 40-41 1.5882318725360918 1108 42-43 -0.29116015753158564 1108 44-45 0.930917836269515 1108 46-47 0.9179025535185872 1108 48-49 1.2429227152356574 1108 50-51 1.1882714592270105 1108 52-53 0.19935374969409025 1108 54-55 -0.3905063439682408 1108 56-57 0.5006149477467403 1108 58-59 0.9630049228212556 1108 60-61 1.2357660025821815 1108 62-63 1.1107337660102345 1108 64-65 1.0590625728665302 1108 66-67 1.1282287692707342 1108 68-69 1.7278997151081867 1108 70-71 -0.4597936041234334 1108 72-73 -0.18516707957235923 1108 74-75 -0.08209912063450986 1108 76 1.1602274841363993 1109 1 -1.540065550618067 1109 2 0.5926073400667846 1109 3 0.44678959567707466 1109 4 0.3085992649355589 1109 5 0.1664296738523987 1109 6 0.01536167639545738 1109 7 0.05083933642312388 1109 8 0.5757565787100845 1109 9 0.7959699730206324 1109 10-11 0.6342771263604092 1109 12-13 0.6791994003208472 1109 14-15 0.4454039908301155 1109 16-17 0.915421091286369 1109 18-19 0.8822575187262345 1109 20-21 1.1334443954391347 1109 22-23 0.7747438004965304 1109 24-25 -0.43146031644231897 1109 26-27 0.3665275148137326 1109 28-29 -0.7400804429776358 1109 30-31 0.7301037328955502 1109 32-33 0.9617818284842841 1109 34-35 0.14519959795229198 1109 36-37 1.4399165392132574 1109 38-39 1.5132495887558832 1109 40-41 1.0153035410691302 1109 42-43 1.4823560798704278 1109 44-45 0.962580770200077 1109 46-47 0.9489775815141144 1109 48-49 0.7132474632759767 1109 50-51 -0.11805552845495981 1109 52-53 -0.09885453698004909 1109 54-55 -0.35761160712613105 1109 56-57 0.3389128200871738 1109 58-59 0.29570369101834615 1109 60-61 1.159926136960678 1109 62-63 0.4438244714973578 1109 64-65 0.7307311059012491 1109 66-67 0.7537886796850657 1109 68-69 0.22274853929631888 1109 70-71 0.8525244249919055 1109 72-73 0.7204320245709752 1109 74-75 0.49399270467344536 1109 76 1.1053002724902612 1110 1 -0.9331106634000186 1110 2 -0.7219290007853445 1110 3 -0.18773421384673838 1110 4 0.23836116969746257 1110 5 0.5883594984138014 1110 6 0.008281475894207802 1110 7 0.9963907148692428 1110 8 0.9714332704394124 1110 9 1.0478496722687467 1110 10-11 -0.5749020716345754 1110 12-13 0.7847131847820066 1110 14-15 1.0183363216571877 1110 16-17 0.9380088105846198 1110 18-19 -0.06592543866725009 1110 20-21 0.941621087168464 1110 22-23 -0.664134645618752 1110 24-25 -1.5699941510287871 1110 26-27 1.2038395448889219 1110 28-29 0.48169273997975637 1110 30-31 -0.20658173076360953 1110 32-33 1.0090249362537094 1110 34-35 0.7091720290299861 1110 36-37 1.8238451106418267 1110 38-39 1.370987683993981 1110 40-41 -0.9828167596827484 1110 42-43 0.1274062051837106 1110 44-45 1.0728251311023342 1110 46-47 0.8997921178549646 1110 48-49 1.3999642552559237 1110 50-51 -0.6627923705602257 1110 52-53 0.8278998489848703 1110 54-55 -0.3577682487301459 1110 56-57 1.1180794867538424 1110 58-59 -0.8859692413124804 1110 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50-51 -0.5388793270819647 2116 52-53 -0.07608565826151192 2116 54-55 -0.0328976483160659 2116 56-57 -0.1632610929563043 2116 58-59 -2.2110468810640356 2116 60-61 -1.5864125358082006 2116 62-63 0.6456551351129178 2116 64-65 -1.510687658400812 2116 66-67 -0.624014523976264 2116 68-69 -1.4223315522368623 2116 70-71 1.4821582922687924 2116 72-73 0.79022607491423 2116 74-75 0.5401025444903738 2116 76 0.04649020384037783 2117 1 0.7371274318380756 2117 2 0.7637852849289359 2117 3 0.18488483377230835 2117 4 0.213361169697464 2117 5 0.46216902222332834 2117 6 0.5785195711323041 2117 7 0.5237716672501946 2117 8 -0.07737625337011167 2117 9 -1.690245565826487 2117 10-11 -1.0993068335393374 2117 12-13 -1.4438582437894283 2117 14-15 -0.4888065354856792 2117 16-17 -1.361395951320148 2117 18-19 -1.8575921053339144 2117 20-21 -0.04349796045058696 2117 22-23 -0.26472988371399 2117 24-25 -1.7664227224573565 2117 26-27 -2.57056521701584 2117 28-29 -3.326045355258337 2117 30-31 -0.6464626831445557 2117 32-33 -3.483832206603431 2117 34-35 0.6579815528395088 2117 36-37 -1.7696072703105514 2117 38-39 -0.3724646969584029 2117 40-41 0.03384990698391732 2117 42-43 -0.5714033186258121 2117 44-45 -1.0730082022309944 2117 46-47 -1.0442555011926515 2117 48-49 -0.2595595542678879 2117 50-51 -0.09374475151260242 2117 52-53 0.019566515651533223 2117 54-55 -0.825030153492051 2117 56-57 0.22224615342049958 2117 58-59 -2.6044216222648657 2117 60-61 -1.035687898127037 2117 62-63 -0.5980301650941229 2117 64-65 -1.6467125031213143 2117 66-67 -0.7792940270818534 2117 68-69 -0.1900334155909036 2117 70-71 -1.6681522667374153 2117 72-73 0.4372240045208571 2117 74-75 1.1966242836208068 2117 76 1.8564280920391312 2118 1 -1.0615780697800474 2118 2 0.603082557235922 2118 3 -1.3891327344200164 2118 4 0.058113828042351656 2118 5 0.1571297249510195 2118 6 0.182403066277935 2118 7 1.097696771272389 2118 8 0.40815310400390103 2118 9 -2.687933961573137 2118 10-11 -0.7610728991888962 2118 12-13 -1.102711688079765 2118 14-15 0.3577954062619 2118 16-17 -4.457419992004382 2118 18-19 -0.9900470290509773 2118 20-21 -0.9632852928592754 2118 22-23 0.45274584444134547 2118 24-25 0.992939274768716 2118 26-27 -3.7598393732802897 2118 28-29 -0.9859159054201498 2118 30-31 -1.565833926787647 2118 32-33 -1.7657092292571477 2118 34-35 -0.6288423029163894 2118 36-37 -3.7008601598158677 2118 38-39 -0.6702455568751802 2118 40-41 -1.2647168983790067 2118 42-43 -0.10566129366048216 2118 44-45 -3.61692868304468 2118 46-47 -0.15909600049917572 2118 48-49 0.21746309945380204 2118 50-51 -3.3399768365796447 2118 52-53 -3.0648995037659432 2118 54-55 -2.044632557560476 2118 56-57 -0.33438815078661577 2118 58-59 -0.9087674382611652 2118 60-61 -1.2020309401982416 2118 62-63 -2.570891931159771 2118 64-65 -0.5984462063113227 2118 66-67 -0.7032884792316381 2118 68-69 -2.1692289773107376 2118 70-71 -0.61239637214657 2118 72-73 1.8467478140446616 2118 74-75 0.6490514680868245 2118 76 -0.8831003406931828 2119 1 -0.09505647620790114 2119 2 -0.7416334342828819 2119 3 -0.8394173008746506 2119 4 -1.4523202752943263 2119 5 -0.8915255590574631 2119 6 -1.0789516932355099 2119 7 -1.9931413048351914 2119 8 -0.5131726408906374 2119 9 -0.24032766763272662 2119 10-11 -1.110308475575458 2119 12-13 -1.808882874331303 2119 14-15 -1.1232892941063568 2119 16-17 -1.0454682009096388 2119 18-19 -0.6999566373536155 2119 20-21 -2.315254939104115 2119 22-23 -1.332545975668019 2119 24-25 0.0833309721239246 2119 26-27 -0.058578353304838515 2119 28-29 -0.40305684951120924 2119 30-31 0.431041421945757 2119 32-33 -0.37233795372986833 2119 34-35 0.23400782541750687 2119 36-37 -1.8301984033154781 2119 38-39 -2.390198687106178 2119 40-41 -2.164672260503764 2119 42-43 -0.35022105261595726 2119 44-45 0.384298858524339 2119 46-47 -0.07824564897590847 2119 48-49 -1.8377204738081119 2119 50-51 0.018242112198393556 2119 52-53 -1.294226587796743 2119 54-55 -0.8890695623590403 2119 56-57 -2.6570641914070876 2119 58-59 -0.8357845122484449 2119 60-61 -1.101205139506348 2119 62-63 -0.7686377184602904 2119 64-65 -0.9025417313643302 2119 66-67 -0.29702801722963557 2119 68-69 0.23279088654374647 2119 70-71 -0.5568222174763342 2119 72-73 0.03065586002167464 2119 74-75 -0.9596975554596519 2119 76 0.18352168809824576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 2.0 21 2.0 22 11.0 23 25.0 24 47.0 25 90.0 26 210.0 27 399.0 28 722.0 29 1079.0 30 1606.0 31 2460.0 32 3760.0 33 5252.0 34 7347.0 35 8823.0 36 12001.0 37 14084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.6366147577777 26.026380304547498 10.931942957770795 29.40506197990401 2 17.2749559752771 18.673388349849798 43.34622423258865 20.705431442284453 3 15.596837125789856 29.00452332447084 33.339663685646215 22.058975864093092 4 11.006180725803667 20.688166845067503 37.20348054279894 31.102171886329895 5 9.94440799696143 42.56586443838265 34.53437381305894 12.955353751596977 6 21.781015848900246 37.412382169123994 17.48558406132385 23.32101792065191 7 21.991643934946996 38.27561202997134 20.304892786851283 19.42785124823038 8 19.144711853872447 33.98535962156003 24.6590242049653 22.210904319602225 9 19.379510376022928 19.74034045785712 19.598770760678153 41.2813784054418 10-11 19.876730775871 24.48551500293498 30.464245019163705 25.173509202030314 12-13 23.31842823106937 20.562135285383793 26.673802700183003 29.445633783363835 14-15 23.745727012188805 25.26673802700183 20.935913815130693 30.05162114567867 16-17 22.846241497185872 27.347121991643935 21.52291012050689 28.283726390663304 18-19 20.196989054245364 27.865059908152347 22.324850661234073 29.613100376368216 20-21 21.196609233106592 25.9754497427575 22.34556817789441 30.482372846241496 22-23 23.917509754497427 29.311833154932494 22.796174165256726 23.974482925313353 24-25 22.492317254238458 31.316252891820035 22.72711577638894 23.46431407755257 26-27 23.28907841580056 32.104381754773655 22.67445875487725 21.93208107454853 28-29 24.19288008010773 27.71226822278236 27.112323469493454 20.982528227616452 30-31 27.710541763060668 24.01160180932979 29.129691654293705 19.148164773315838 32-33 28.69289734470495 23.308069472739202 28.830150892579674 19.168882289976175 34-35 26.794654880701636 27.032043092434655 27.460205103414935 18.713096923448777 36-37 21.692966403093816 26.74286108905079 28.46155174199786 23.10262076585753 38-39 21.490970615655534 24.572701218880564 27.568972065881702 26.367356099582196 40-41 20.085632402196058 26.442457097475913 29.613963606229067 23.857946894098962 42-43 23.54891060391561 25.233072062428786 31.034839957183795 20.183177376471807 44-45 20.037291529988604 25.190773799247264 27.866786367874035 26.90514830289009 46-47 23.56962812057595 22.766824349987917 27.816719035944892 25.84682849349125 48-49 21.552259935775698 22.626117882669796 29.63209143330686 26.18953074824764 50-51 18.01301750630158 22.80739615344774 32.038776285349265 27.140810054901422 52-53 20.59234833051345 22.688270432650807 34.01470943682884 22.704671800006906 54-55 19.176651358723802 22.682227823624874 32.38493145954905 25.756189358102276 56-57 22.495770173681848 22.76509789026622 30.65415558855012 24.08497634750181 58-59 19.328579814232935 30.148302890093575 29.623459134698386 20.899658160975104 60-61 19.08946514277822 36.276371672248885 24.465660716135492 20.168502468837403 62-63 16.607679292842096 39.44010911225441 23.313248851904287 20.638962742999205 64-65 16.58696177618176 40.29643313421498 22.3636960049722 20.752909084631057 66-67 16.55761196091295 39.572183280964055 22.352474016781187 21.517730741341804 68-69 16.321086979040782 37.70329063222955 22.93342771313145 23.042194675598218 70-71 17.228341562791343 35.56938641621491 23.091398777666516 24.110873243327234 72-73 17.678084320292808 33.5710092883533 23.023203618659576 25.72770277269431 74-75 17.779945443872794 33.29909188218639 23.1129795241877 25.807983149753117 76 18.001795518110562 33.256793619004874 23.182901142916336 25.55850971996823 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 3.0 20 8.0 21 11.0 22 11.0 23 32.5 24 55.5 25 57.0 26 82.5 27 133.5 28 222.5 29 286.0 30 312.5 31 413.0 32 643.5 33 800.0 34 905.0 35 1197.5 36 1441.0 37 1497.0 38 1774.5 39 2347.0 40 3068.0 41 4051.5 42 4609.0 43 5245.5 44 6021.5 45 5825.5 46 5490.0 47 5099.5 48 4270.0 49 3453.5 50 3076.0 51 2779.5 52 2188.0 53 1703.0 54 1513.0 55 1438.5 56 1131.0 57 766.5 58 635.0 59 488.0 60 284.0 61 193.5 62 160.0 63 135.5 64 90.0 65 45.0 66 22.5 67 24.0 68 22.0 69 11.0 70 3.0 71 4.0 72 2.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 57922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.86647560512413 #Duplication Level Percentage of deduplicated Percentage of total 1 69.11147130332277 32.39011083871413 2 13.552641273115745 12.70329063222955 3 6.483459809916746 9.115707330547979 4 3.484859647830251 6.532923586892718 5 2.099756870257128 4.9204102068298745 6 1.4256244013851027 4.008839473775077 7 1.0388270831798423 3.4080314906253237 8 0.6446621970087674 2.4170436103725703 9 0.48257570176084874 2.0354960118780427 >10 1.613497384513372 10.812817236973862 >50 0.03315405584616518 1.113566520493077 >100 0.018418919914536213 1.612513380062843 >500 0.0 0.0 >1k 0.011051351948721727 8.929249680604952 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT 2916 5.034356548461724 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 1247 2.152895272953282 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT 1009 1.7419978591899452 No Hit CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 358 0.6180725803667001 No Hit TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT 202 0.34874486378232794 No Hit CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 128 0.2209868443769207 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT 124 0.21408100549014192 No Hit CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 122 0.2106280860467525 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT 100 0.17264597216946928 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT 92 0.15883429439591173 No Hit CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 79 0.13639031801388074 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 71 0.12257864024032321 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 68 0.11739926107523911 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT 68 0.11739926107523911 No Hit CTTATCACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 59 0.10186112357998688 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0017264597216946928 0.0 2 0.0 0.0 0.0 0.0034529194433893856 0.0 3 0.0 0.0 0.0 0.005179379165084079 0.0 4 0.0 0.0 0.0 0.005179379165084079 0.0 5 0.0 0.0 0.0 0.005179379165084079 0.0 6 0.0 0.0 0.0 0.005179379165084079 0.0 7 0.0 0.0 0.0 0.005179379165084079 0.0 8 0.0 0.0 0.0 0.005179379165084079 0.0 9 0.0 0.0 0.0 0.005179379165084079 0.0 10 0.0 0.0 0.0 0.006905838886778771 0.0 11 0.0 0.0 0.0 0.006905838886778771 0.0 12 0.0 0.0 0.0 0.006905838886778771 0.0 13 0.0 0.0 0.0 0.006905838886778771 0.0 14 0.0 0.0 0.0 0.006905838886778771 0.0 15 0.0 0.0 0.0 0.006905838886778771 0.0 16 0.0 0.0 0.0 0.006905838886778771 0.0 17 0.0 0.0 0.0 0.006905838886778771 0.0 18 0.0 0.0 0.0 0.010358758330168158 0.0 19 0.0 0.0 0.0 0.010358758330168158 0.0 20 0.0 0.0 0.0 0.010358758330168158 0.0 21 0.0 0.0 0.0 0.010358758330168158 0.0 22 0.0 0.0 0.0 0.013811677773557542 0.0 23 0.0 0.0 0.0 0.013811677773557542 0.0 24 0.0 0.0 0.0 0.013811677773557542 0.0 25 0.0 0.0 0.0 0.013811677773557542 0.0 26 0.0 0.0 0.0 0.015538137495252236 0.0 27 0.0 0.0 0.0 0.01726459721694693 0.0 28 0.0 0.0 0.0 0.02244397638203101 0.0 29 0.0 0.0 0.0 0.03107627499050447 0.0 30 0.0 0.0 0.0 0.04661441248575671 0.0 31 0.0 0.0 0.0 0.06042609025931425 0.0 32 0.0 0.0 0.0 0.07941714719795587 0.0 33 0.0 0.0 0.0 0.0966817444149028 0.0 34 0.0 0.0 0.0 0.11221988191015504 0.0 35 0.0 0.0 0.0 0.13121093884879667 0.0 36 0.0 0.0 0.0 0.18127827077794276 0.0 37 0.0 0.0 0.0 0.32284796795690757 0.0 38 0.0 0.0 0.0 0.5075791581782397 0.0 39 0.0 0.0 0.0 0.70266910672974 0.0 40 0.0 0.0 0.0 0.9219294913849659 0.0 41 0.0 0.0 0.0 1.1964365871344222 0.0 42 0.0 0.0 0.0 1.5261903939781085 0.0 43 0.0 0.0 0.0 1.9146438313594143 0.0 44 0.0 0.0 0.0 2.2633886951417423 0.0 45 0.0 0.0 0.0 2.6276716964193225 0.0 46 0.0 0.0 0.0 2.9885017782535135 0.0 47 0.0 0.0 0.0 3.378681675356514 0.0 48 0.0 0.0 0.0 3.7395117571907046 0.0 49 0.0 0.0 0.0 4.074444943199476 0.0 50 0.0 0.0 0.0 4.455992541694003 0.0 51 0.0 0.0 0.0 4.916957287386485 0.0 52 0.0 0.0 0.0 5.333034080314906 0.0 53 0.0 0.0 0.0 5.718034598252823 0.0 54 0.0 0.0 0.0 6.061600082870067 0.0 55 0.0 0.0 0.0 6.418977245260868 0.0 56 0.0 0.0 0.0 6.831601118745899 0.0 57 0.0 0.0 0.0 7.182072442249923 0.0 58 0.0 0.0 0.0 7.525637926867166 0.0 59 0.0 0.0 0.0 7.889920928144746 0.0 60 0.0 0.0 0.0 8.254203929422326 0.0 61 0.0 0.0 0.0 8.592590034874487 0.0 62 0.0 0.0 0.0 9.034563723628327 0.0 63 0.0 0.0 0.0 9.438555298504886 0.0 64 0.0 0.0 0.0 9.81147059839094 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTCCT 15 0.0022142204 70.0 50 GTCTGCC 20 7.865301E-5 70.0 39 GCACACG 15 0.0022142204 70.0 15 CTGCCGT 20 7.865301E-5 70.0 41 GCGGCTG 15 0.0022142204 70.0 10 CTTTATA 20 7.865301E-5 70.0 2 CTTTACA 200 0.0 59.500004 1 TTTACAC 215 0.0 56.976746 2 TCTGCCG 25 2.372057E-4 56.000004 40 CGTCTGC 25 2.372057E-4 56.000004 38 TTACACA 220 0.0 54.090908 3 TTGGCAG 20 0.0069189076 52.5 39 GGCAAAA 20 0.0069189076 52.5 65 AGCGGCT 20 0.0069189076 52.5 9 TACGAGT 20 0.0069189076 52.5 4 TCTCCTG 20 0.0069189076 52.5 50 CGAGCAC 20 0.0069189076 52.5 12 GGGCAAA 20 0.0069189076 52.5 64 TGGGCAA 20 0.0069189076 52.5 63 CTCTCGT 20 0.0069189076 52.5 34 >>END_MODULE