FastQCFastQC Report
Wed 25 May 2016
SRR1780385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223734
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTGC250.002357629135.17280230
GCTCTAA353.211452E-431.4253731
GTCTATG353.2325956E-431.3902442
CGCGTGG353.2325956E-431.3902442
GCGACCT300.005719162529.35005834
GTGATTC300.005719162529.35005840
CGAGCAA300.005719162529.35005837
CGTCTAT454.0576997E-529.330351
ACGTCGT300.00575683429.31066930
AGCTCCA554.915468E-628.01596336
GACGACC406.9268874E-427.55888420
CAATCGG406.9724466E-427.5280122
GTACATT406.9907395E-427.51567638
GTGCTTC406.9907395E-427.51567637
CACCCAT406.999901E-427.50951611
ATGTATT407.055076E-427.47260729
CCGCCGA655.79701E-727.12881718
CAAGCTC655.865568E-727.0923634
CAATGTG508.355425E-526.37960232
GCTCCAT609.6637505E-625.68130137