FastQCFastQC Report
Wed 25 May 2016
SRR1780385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223734
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT183438.19857509363798Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC76393.4143223649512366No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC24261.0843233482617751RNA PCR Primer, Index 47 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT15710.7021731162898799No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT13160.5881984857017708No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG7710.34460564777816516No Hit
CTTAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC7300.32628031501693977No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC7200.3218107216605433No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5910.2641529673630293No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC5310.2373354072246507No Hit
CTTATCACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3760.16805671020050594RNA PCR Primer, Index 47 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3610.1613523201659113RNA PCR Primer, Index 47 (95% over 22bp)
CTTATTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG3310.147943540096722No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2940.1314060446780552No Hit
ACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTTCT2750.12291381730090198Illumina PCR Primer Index 9 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT2680.11978510195142446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAACT254.4222805E-544.01520516
CAGCGTC254.428124E-544.0053632
AGCGTCA254.428124E-544.0053633
TCCTATA207.838164E-444.0053632
CTTATAA551.8189894E-1244.0053631
TCTAACT207.846801E-443.99553321
AGTTAGA207.846801E-443.99553336
CCGTCTT19350.042.9723844
CTATACA2500.039.6048281
TTCAGCC501.3387762E-939.59597444
TTTACAC8700.039.4530872
GCCGTCT21300.039.03828443
CTTTACA9400.036.749161
CGCCAAC301.2951098E-436.67933715
GCGACAG301.2985231E-436.6629435
GATTTCT301.2985231E-436.6629443
GTAGCCA301.2985231E-436.6629422
AGCGGAT553.4015102E-935.99633835
CTTTATA1050.035.623392
TCGGCGT250.002344729635.21216211
Produced by FastQC (version 0.11.5)