FastQCFastQC Report
Wed 25 May 2016
SRR1780384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33162
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC150.002206784269.9924554
AGTTGAA200.00689586452.49434331
TATAGTT200.00689586452.49434348
CAACTGA200.00689586452.4943436
ATTTGAG200.00689586452.49434339
ATTTTAT305.799795E-446.66163643
TCAGTAC305.799795E-446.66163640
TTTACAC350.001238869339.9956932
CTCCAAC500.006856189628.2097261
TCCAACA500.00711512227.9969842
TCATCCT500.00711512227.9969849
Produced by FastQC (version 0.11.5)