FastQCFastQC Report
Wed 25 May 2016
SRR1780384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33162
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT31059.363126470056088No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT12403.7392195886858453No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC11853.5733671069296182No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC4271.2876183583619807No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT2690.8111694107713648No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1140.3437669621856342No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT1130.3407514625173391No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1080.325673964175864No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC950.2864724684880285No Hit
CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC900.2713949701465533No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG830.250286472468488No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC800.2412399734636029No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT770.23219347445871782No Hit
CTTATCACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC730.22013147578553768No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT700.21108497678065255No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT630.1899764791025873No Hit
TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC590.17791448042940716No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG580.17489898076111213No Hit
CTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT570.1718834810928171No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT520.1568059827513419No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC420.12665098606839154No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTC410.1236354864000965No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG390.11760448706350643No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT370.11157348772691635No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTCTCTCGTATGCCGTCTT340.10252698872203125No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAAG150.002205841570.015
GTATTCT150.002205841570.048
CTATACA1350.064.814811
CTTTACA1400.060.01
TTTACAC1450.057.9310342
AAACCAC252.3572077E-455.99999667
GGTTGGC200.006892927552.59
CTTTATA200.006892927552.52
TGATTTT200.006892927552.520
CTTATAA200.006892927552.51
TTGGCTA200.006892927552.511
ATTCTGC200.006892927552.550
TTAATAC200.006892927552.52
TCTTTAT200.006892927552.51
CGCGCCC200.006892927552.515
CCGCGCC200.006892927552.514
GAAAAAC305.796717E-446.66666460
ATAACAC305.796717E-446.6666644
TTACACA1850.043.5135153
CTCTCCG350.001238213540.07
Produced by FastQC (version 0.11.5)