FastQCFastQC Report
Wed 25 May 2016
SRR1780383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423263
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG250.002353704835.1919630
TATAGAC353.2388064E-431.3879853
TAAGGCC555.001075E-627.963844
ACGTCTG407.077788E-427.4644878
CTGCCGT508.25492E-526.43460815
GTCTAAC450.001399536724.4359681
CGTTCAT450.001404365124.42153242
TTAACGG651.8013929E-523.70651835
TTTACAC2250.023.4363612
GTGCCAG500.002554631822.0340621
AATCGGA1004.5425622E-822.02884117
TGCTACC703.1941534E-522.01319938
TAATTCA500.00256872622.01319734
GTCTGAC500.002577567222.00017710
GGTACAA602.8752367E-421.9923741
CTTTACA2250.021.5036541
CGGTGGT2000.020.8804143
GTGGCAC856.0912844E-620.708523
TGGCAAC856.1100272E-620.7011529
AATACCC856.166558E-620.6791425
Produced by FastQC (version 0.11.5)