FastQCFastQC Report
Wed 25 May 2016
SRR1780383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423263
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT351278.299095361512817No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT316607.479982894795907Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC106782.522781343987072No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC54081.2776925930213603No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG40920.9667747948674938No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT31640.7475257700295088No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC18190.4297564398494553No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC17180.40589420762032113No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG13880.3279284983568136No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC10370.24500133486744646No Hit
CTTATAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG10090.23838606256630038No Hit
CTTAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC9280.2192490248379849No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC8670.20483718161048806No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG7390.17459593680524874No Hit
CTTATTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG6220.1469535489754597No Hit
CTTATCACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC6130.1448272114500913No Hit
CTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT4590.1084432137937878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT34200.042.25873644
TTTACAC11550.038.6671872
GCCGTCT38900.037.1529243
CTATACA7300.036.798051
CTTTACA12250.036.3080671
GCCGTTA250.002348191635.20879415
CGTGTCG250.002350916235.2004749
GTAGCCG250.002352279435.19631225
TACCGCG250.002352279435.1963128
TTACACA13150.034.1297383
CTTATAA1100.034.02861
CTTTATA2800.032.2147182
CGCCATT551.3714089E-731.99664717
TCTTTAT2850.031.6757451
ATCTATA353.2163312E-431.42528223
GACCCGT353.2163312E-431.42528223
CCGTTTG353.2163312E-431.42528226
CTTATAC49700.031.3664021
TTATACA51200.030.5082232
ATAACAC1250.029.9203994
Produced by FastQC (version 0.11.5)