FastQCFastQC Report
Wed 25 May 2016
SRR1780382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8057
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT228328.335608787389848Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT91811.393819039344669No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC7589.407968226386993No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC2793.462827355094948No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT1842.283728434901328No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG750.9308675685739108No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG700.8688097306689836No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC680.8439865955070125No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT650.8067518927640561No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG640.7943403251830705No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC600.7446940548591287No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTCTT550.6826362169542013No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT530.6578130817922303No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC510.6329899466302593No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT470.5833436763063176No Hit
CTTATCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC350.4344048653344918No Hit
TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC340.42199329775350625No Hit
CTTATTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG330.4095817301725208No Hit
ACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCT280.3475238922675934Illumina PCR Primer Index 2 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT270.33511232468660795No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT260.32270075710562246No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTCTGCCGTCTT260.32270075710562246No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG250.31028918952463697No Hit
CTTATACACATCTCCGAGCCCACGCGACCCGTTTGTATCTCGTATGCCGT240.29787762194365147No Hit
CTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT230.285466054362666No Hit
ATACACATCTCCGAGCCCACGAGACCCGATTGTATCTCGTATGCCGTCTT220.2730544867816805RNA PCR Primer, Index 35 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC220.2730544867816805No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTCTCTCGTATGCCGTCTT220.2730544867816805No Hit
CTTATATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC200.24823135161970958No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGT180.22340821645773862No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGTTTGTATCTCGTATGCCGT180.22340821645773862No Hit
ATACACATCTCCGAGCCCACGAGAACCGTTTGTATCTCGTATGCCGTCTT170.21099664887675312TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCGTCTGTATCTCGTATGCCGTCTT170.21099664887675312Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGTTTGTATCTCGTATGCCGTCTT160.19858508129576763TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCGCTTGTATCTCGTATGCCGTCTT160.19858508129576763TruSeq Adapter, Index 8 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTC150.18617351371478216No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTAGTATGCCGT140.1737619461337967No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTC140.1737619461337967No Hit
ATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGTCTT140.1737619461337967TruSeq Adapter, Index 2 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT140.1737619461337967No Hit
TCTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT130.16135037855281123No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTC120.14893881097182574No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTCGTATCTCGTATGCCGTCTT120.14893881097182574RNA PCR Primer, Index 27 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCAGTCTT120.14893881097182574No Hit
ATACACATCTCCGCGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT120.14893881097182574Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCCT120.14893881097182574No Hit
CTTATACACATCTCCGAGCCCAAGAGACCCGTTTGTATCTCGTATGCCGT120.14893881097182574No Hit
ATACACATCTACGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT120.14893881097182574Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGCCCCGTTTGTATCTCGTATGCCGTCTT110.13652724339084024Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTC100.12411567580985479No Hit
CTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTGCTTGA100.12411567580985479Illumina PCR Primer Index 2 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCT100.12411567580985479No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCCCGTATGCCGT100.12411567580985479No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTATT90.11170410822886931No Hit
CTTATACCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG90.11170410822886931No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT90.11170410822886931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT150.002145292770.01
TCTTAAT150.002145292770.01
CTTTACA1200.064.1666641
TTTACAC1250.061.62
CTATACA408.998541E-961.251
TTACACA1350.057.0370373
TTTATAC252.2507997E-455.9999963
CAAACAC252.2507997E-455.99999669
CTTATAC2050.052.926831
CTTTATA200.00670514452.52
GACCCGA200.00670514452.523
TTATACA2200.049.318182
ACAAACA305.5365666E-446.66666468
TATACAC2900.038.620693
TCTTATA501.5646413E-435.01
ATACACA6400.033.906251
GTATCTC6350.031.41732432
AACAAAC450.004061597431.11111367
TATCTCG6350.030.86614233
TGTATCT6450.030.38759831
Produced by FastQC (version 0.11.5)