##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780382_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8057 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50527491622192 34.0 34.0 34.0 31.0 34.0 2 33.02147201191511 34.0 34.0 34.0 31.0 34.0 3 33.341566339828724 34.0 34.0 34.0 33.0 34.0 4 33.46158619833685 34.0 34.0 34.0 33.0 34.0 5 33.46146208266104 34.0 34.0 34.0 34.0 34.0 6 37.221050018617355 38.0 37.0 38.0 36.0 38.0 7 36.92342062802532 38.0 37.0 38.0 35.0 38.0 8 36.87240908526747 38.0 37.0 38.0 35.0 38.0 9 36.404617103140126 38.0 37.0 38.0 34.0 38.0 10-11 36.08675685739109 38.0 36.5 38.0 32.5 38.0 12-13 35.77969467543751 38.0 36.5 38.0 31.5 38.0 14-15 35.586446568201566 38.0 36.0 38.0 31.0 38.0 16-17 35.7304828099789 38.0 36.0 38.0 31.0 38.0 18-19 35.926027057217325 38.0 36.5 38.0 31.5 38.0 20-21 35.820032270075714 38.0 36.0 38.0 31.0 38.0 22-23 35.382958917711306 37.5 35.0 38.0 29.0 38.0 24-25 34.10574655578999 37.0 32.5 38.0 24.0 38.0 26-27 34.491932481072354 37.0 34.0 38.0 26.0 38.0 28-29 33.59923048280997 37.0 32.5 38.0 23.5 38.0 30-31 32.76498696785404 36.5 31.5 38.0 21.5 38.0 32-33 33.30408340573415 37.0 33.5 38.0 22.5 38.0 34-35 32.28602457490381 36.0 30.5 37.5 17.0 38.0 36-37 32.38258657068388 36.0 31.5 37.0 21.0 38.0 38-39 31.91485664639444 36.0 31.0 37.0 11.0 38.0 40-41 32.848827106863595 36.5 32.5 38.0 17.0 38.0 42-43 34.37873898473377 37.0 34.0 38.0 26.0 38.0 44-45 34.80886185925283 37.0 34.5 38.0 27.0 38.0 46-47 34.503599354598485 37.0 34.0 38.0 26.0 38.0 48-49 34.031959786521035 37.0 33.5 38.0 25.0 38.0 50-51 34.20590790616855 37.0 34.0 38.0 25.5 38.0 52-53 33.69883331264739 37.0 34.0 38.0 24.0 38.0 54-55 34.07757229738116 37.0 34.0 38.0 25.5 38.0 56-57 33.854350254437136 37.0 34.0 38.0 24.0 38.0 58-59 33.679160978031526 37.0 34.0 38.0 24.0 38.0 60-61 33.623246866079185 37.0 34.0 38.0 22.5 38.0 62-63 34.560444334119396 37.0 34.0 38.0 27.0 38.0 64-65 34.46549584212486 37.0 34.0 38.0 26.0 38.0 66-67 31.93744569939183 36.0 31.0 38.0 16.0 38.0 68-69 26.30805510736006 28.0 16.0 36.5 10.0 38.0 70-71 22.304331637085767 22.5 11.0 31.5 10.0 35.5 72-73 19.573228248727816 20.0 11.0 25.0 11.0 34.0 74-75 19.187973191014024 19.0 11.0 25.0 11.0 34.0 76 19.42621323073104 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2031666330199755 1101 2 0.217453807595831 1101 3 -0.3485401622094102 1101 4 -0.5856981329362583 1101 5 -0.17487682261881332 1101 6 -0.9726537930582424 1101 7 -0.05271379782526964 1101 8 0.6054886821070014 1101 9 1.147921492770756 1101 10-11 -0.9805385351283533 1101 12-13 0.36462300820358706 1101 14-15 1.8820983117616876 1101 16-17 1.2642076043944144 1101 18-19 -0.23392848576101244 1101 20-21 0.8798486300271691 1101 22-23 0.6922656164862104 1101 24-25 -2.4110162990442667 1101 26-27 -0.3972746867015289 1101 28-29 0.260956072848586 1101 30-31 1.2228586928381162 1101 32-33 -0.8702855421226019 1101 34-35 0.3500175565832748 1101 36-37 0.9047291489859646 1101 38-39 0.018553597926363352 1101 40-41 2.2739559215262872 1101 42-43 1.867889303235522 1101 44-45 1.499815335645465 1101 46-47 2.030404944872231 1101 48-49 -3.2703852644381612 1101 50-51 -2.9566060609744937 1101 52-53 -2.9013899808534696 1101 54-55 -1.0407549383208945 1101 56-57 -2.8761704875102296 1101 58-59 -3.5561807876540428 1101 60-61 -2.689914739104964 1101 62-63 -2.5771215174754616 1101 64-65 -3.0822466972995386 1101 66-67 -5.167376885255365 1101 68-69 1.6620158195400592 1101 70-71 1.8951531395888175 1101 72-73 -0.9249676552031119 1101 74-75 0.8487709731478716 1101 76 1.9642582682556196 1102 1 -2.532836962690304 1102 2 -0.4089198187777896 1102 3 -0.3815071951764395 1102 4 0.23847769123956652 1102 5 0.4130352652932743 1102 6 0.6921813717769183 1102 7 0.10113235602088366 1102 8 0.8280161546344686 1102 9 0.5105588554081208 1102 10-11 0.5304504758606612 1102 12-13 1.3701175136980979 1102 14-15 0.008471938135308221 1102 16-17 0.01970210988892518 1102 18-19 -0.7668955187280488 1102 20-21 0.9677607179392638 1102 22-23 1.6689139681345608 1102 24-25 -4.787389925417894 1102 26-27 -1.5140329284597769 1102 28-29 -1.4107472238547132 1102 30-31 3.2132433082227294 1102 32-33 -0.2672635641006238 1102 34-35 2.63710546867118 1102 36-37 -2.004611510354689 1102 38-39 0.013059092431852548 1102 40-41 1.1228570204273893 1102 42-43 2.200306885653106 1102 44-45 2.380309841139976 1102 46-47 3.254306043773333 1102 48-49 -2.767638011690906 1102 50-51 -0.5664961708646032 1102 52-53 -1.117049321512809 1102 54-55 1.5924868199208575 1102 56-57 2.741961380621639 1102 58-59 -0.663323644796904 1102 60-61 -5.331398255588482 1102 62-63 -1.2144841548380967 1102 64-65 -0.1674115324643708 1102 66-67 2.768062675184197 1102 68-69 -0.009687477163240032 1102 70-71 -0.5100666406309635 1102 72-73 -3.0046379848734404 1102 74-75 0.5122325116094117 1102 76 -0.7774999735026213 1103 1 0.967163037309696 1103 2 0.8767944669364951 1103 3 0.2256356619664146 1103 4 0.3099062626681359 1103 5 -0.12267902042101042 1103 6 -0.0578186282230817 1103 7 0.49398949887802956 1103 8 -0.10055527393695485 1103 9 0.5819874268366902 1103 10-11 -1.7195495241393388 1103 12-13 -1.4334539148733327 1103 14-15 0.3299005095638847 1103 16-17 1.0554163956032099 1103 18-19 0.46524733841480526 1103 20-21 -0.42509642491788924 1103 22-23 0.9724853967059914 1103 24-25 -3.0731042111321827 1103 26-27 -0.6926043570312075 1103 28-29 0.8213956332881409 1103 30-31 1.2311004510798682 1103 32-33 2.000593578756522 1103 34-35 1.3335340400997566 1103 36-37 0.9239599182167382 1103 38-39 -0.07622662185386275 1103 40-41 -1.3771429795726107 1103 42-43 -0.7461216857754636 1103 44-45 1.9160241268542606 1103 46-47 2.290020329487618 1103 48-49 -4.267638011690906 1103 50-51 0.09421811484968146 1103 52-53 -0.5991921786556702 1103 54-55 0.5746296770637187 1103 56-57 0.9383899520502155 1103 58-59 -1.6276093590826122 1103 60-61 -5.188541112731343 1103 62-63 -1.9644841548380967 1103 64-65 0.2433027532499068 1103 66-67 -2.4283658962443724 1103 68-69 0.4903125228367635 1103 70-71 0.7935047879404635 1103 72-73 -1.415352270587725 1103 74-75 -1.5591960598191577 1103 76 0.47250002649737866 1104 1 0.7814487515954127 1104 2 0.4482230383650645 1104 3 -0.8434119570812015 1104 4 -0.34723659447471533 1104 5 0.022559074817088742 1104 6 0.13503851463406136 1104 7 0.5011323560208822 1104 8 0.9542066308249488 1104 9 1.5248445696938333 1104 10-11 -0.4100257146155357 1104 12-13 1.14154608512667 1104 14-15 1.4179957476591198 1104 16-17 0.9911306813174932 1104 18-19 0.7711997193671891 1104 20-21 -0.42271547253692887 1104 22-23 1.1653425395631274 1104 24-25 1.086419598391629 1104 26-27 1.73477659534975 1104 28-29 2.090443252335767 1104 30-31 0.9549099748893966 1104 32-33 1.8720221501850887 1104 34-35 -2.5307516741859573 1104 36-37 3.6572932515500725 1104 38-39 3.024963854336619 1104 40-41 1.975237972808337 1104 42-43 0.9717354570816781 1104 44-45 0.843405079235211 1104 46-47 3.015020329487619 1104 48-49 1.1680762740233845 1104 50-51 1.138265733897299 1104 52-53 1.662712583249089 1104 54-55 1.557963010397053 1104 56-57 1.0943423330025865 1104 58-59 -1.5561807876540428 1104 60-61 0.5293160301258055 1104 62-63 0.5164682261142843 1104 64-65 2.0754456103927694 1104 66-67 1.4454436275651474 1104 68-69 3.2748363323605716 1104 70-71 4.172076216511893 1104 72-73 1.8929810627456085 1104 74-75 -0.8461008217239225 1104 76 0.1129762169735713 1105 1 0.8632669334135912 1105 2 0.7664048565468846 1105 3 0.5990122853430364 1105 4 0.36185431461618833 1105 5 0.07104392330193576 1105 6 0.3198869994825486 1105 7 0.7981020529905791 1105 8 -0.9306418540235342 1105 9 0.906662751512016 1105 10-11 1.279368224778409 1105 12-13 -0.28572664214605936 1105 14-15 1.1664805961439697 1105 16-17 0.5305246207114322 1105 18-19 0.8121088102762783 1105 20-21 0.3984966486751844 1105 22-23 1.1759486001691926 1105 24-25 1.7970256589976898 1105 26-27 0.7938675044406622 1105 28-29 2.061655373547886 1105 30-31 2.1185463385257606 1105 32-33 1.0720221501850915 1105 34-35 -0.14590318933747426 1105 36-37 1.105778100034918 1105 38-39 -2.01291493354217 1105 40-41 -0.6444589968886234 1105 42-43 1.1277960631422843 1105 44-45 1.5918899277200609 1105 46-47 2.263505177972462 1105 48-49 -0.12586311991601207 1105 50-51 1.4670536126851772 1105 52-53 1.8672580377945422 1105 54-55 1.2564478588818986 1105 56-57 1.2094938481541035 1105 58-59 -0.04102927250252719 1105 60-61 1.4808311816409514 1105 62-63 -1.3183802587342015 1105 64-65 -0.19879681384965409 1105 66-67 0.989383021504544 1105 68-69 1.1672605747848124 1105 70-71 2.5402580346937143 1105 72-73 1.489950759715306 1105 74-75 -0.014282639905740524 1105 76 0.42812773212508404 1106 1 -0.1647050945584354 1106 2 0.7559153460573711 1106 3 0.5745367608675167 1106 4 -0.047236594474718174 1106 5 -0.6748768226188133 1106 6 -0.241884562289016 1106 7 -1.2834830285945031 1106 8 0.8362579128762277 1106 9 0.10945995430922295 1106 10-11 0.711769157179333 1106 12-13 1.787699931280514 1106 14-15 0.07440600406937392 1106 16-17 0.4372845274713342 1106 18-19 1.3622253603928272 1106 20-21 1.3990793992579427 1106 22-23 2.2307271549477434 1106 24-25 1.3005221624941896 1106 26-27 1.7181099286830843 1106 28-29 0.8378791497716662 1106 30-31 1.1843971543765761 1106 32-33 0.6104836886466316 1106 34-35 2.176940633506348 1106 36-37 3.712421456678271 1106 38-39 -0.03913870976595035 1106 40-41 2.7162636138339806 1106 42-43 1.6371200724662884 1106 44-45 2.057507643337779 1106 46-47 2.780404944872231 1106 48-49 3.3257685817156855 1106 50-51 1.3126247082562728 1106 52-53 1.925533096069607 1106 54-55 0.0938604462944852 1106 56-57 1.6238295124897704 1106 58-59 2.48228075080749 1106 60-61 2.7908544916642626 1106 62-63 2.692109251755312 1106 64-65 1.1485225334696878 1106 66-67 3.582623114744635 1106 68-69 4.431246588770829 1106 70-71 5.279768524204201 1106 72-73 0.8634938832584282 1106 74-75 1.0218478962247985 1106 76 1.425796729794083 1107 1 1.014782084928747 1107 2 0.14822303836506734 1107 3 0.48992137625212706 1107 4 0.4527634055252818 1107 5 -0.6774409251829141 1107 6 0.03503851463406704 1107 7 0.8344656893542179 1107 8 0.6208732974916131 1107 9 -1.0084887636394981 1107 10-11 -0.26002571461553714 1107 12-13 -1.2584539148733356 1107 14-15 0.5846624143257912 1107 16-17 0.8411306813174946 1107 18-19 0.837866386033852 1107 20-21 0.5439511941297326 1107 22-23 -0.26799079377020263 1107 24-25 -0.6635804016083711 1107 26-27 -1.9152234046502556 1107 28-29 -0.05955674766423158 1107 30-31 1.0882433082227294 1107 32-33 1.3886888168517544 1107 34-35 0.16924832581403848 1107 36-37 2.8906265848834067 1107 38-39 1.2749638543366117 1107 40-41 0.9419046394750055 1107 42-43 1.6050687904150038 1107 44-45 0.7100717459018782 1107 46-47 2.1983536628209492 1107 48-49 0.9847429406900474 1107 50-51 2.1049324005639676 1107 52-53 1.4960459165824247 1107 54-55 1.6079630103970501 1107 56-57 2.627675666335925 1107 58-59 0.8438192123459558 1107 60-61 1.9459826967924698 1107 62-63 1.0164682261142843 1107 64-65 1.1421122770594323 1107 66-67 -1.837889705768184 1107 68-69 -2.7918303343060913 1107 70-71 -0.8612571168214416 1107 72-73 -0.1570189372543922 1107 74-75 -0.24610082172392111 1107 76 0.346309550306902 1108 1 -1.8899798198331617 1108 2 0.2339373240793492 1108 3 -1.1672214808907242 1108 4 0.16704911981099713 1108 5 -0.05125044899243392 1108 6 -0.7363900567945052 1108 7 -0.8631533582648316 1108 8 -1.1719838453655314 1108 9 -0.41801257316330975 1108 10-11 0.7447361901463694 1108 12-13 1.9415460851266673 1108 14-15 0.8656147952781694 1108 16-17 -2.8017264615396513 1108 18-19 -0.6061812330137641 1108 20-21 1.057046432224972 1108 22-23 1.2939139681345608 1108 24-25 1.605467217439248 1108 26-27 -0.996175785602631 1108 28-29 -2.375032938140425 1108 30-31 -0.7153281203487012 1108 32-33 0.8577364358993762 1108 34-35 0.13710546867118012 1108 36-37 2.191817061073877 1108 38-39 -0.7369409075681475 1108 40-41 0.6407141632845352 1108 42-43 0.539592599938814 1108 44-45 1.3088812697114065 1108 46-47 2.1828774723447637 1108 48-49 2.768076274023379 1108 50-51 1.8620752577068274 1108 52-53 0.7400935356300451 1108 54-55 0.3246296770637187 1108 56-57 0.38481852347877776 1108 58-59 0.3188192123459572 1108 60-61 1.5078874586972333 1108 62-63 1.9998015594476186 1108 64-65 0.6718741818213374 1108 66-67 1.268062675184197 1108 68-69 4.418883951408191 1108 70-71 1.7577905022261788 1108 72-73 2.6382191579837055 1108 74-75 0.9408039401808423 1108 76 -1.2417856877883366 1109 1 0.0028773230239806935 1109 2 0.5910801812222104 1109 3 0.3327785191092758 1109 4 -0.011522308760433475 1109 5 -0.015536163278149218 1109 6 -0.1649614853659358 1109 7 -0.29172478683626224 1109 8 0.47087329749161455 1109 9 0.867701712550982 1109 10-11 0.7447361901463694 1109 12-13 0.08440322798380606 1109 14-15 0.8477576524210235 1109 16-17 1.2339878241746334 1109 18-19 0.9652473384148124 1109 20-21 1.8963321465106873 1109 22-23 -0.11680031757973097 1109 24-25 -1.8409613539893215 1109 26-27 -1.1390329284597769 1109 28-29 -0.3750329381404214 1109 30-31 0.6775290225084447 1109 32-33 1.4827364358993762 1109 34-35 -2.6128945313288163 1109 36-37 1.1025313467881688 1109 38-39 0.7273448067175678 1109 40-41 0.10499987757024343 1109 42-43 1.6645925999388211 1109 44-45 2.0588812697114065 1109 46-47 0.9328774723447566 1109 48-49 1.4109334168805177 1109 50-51 -0.5307818851503185 1109 52-53 0.2758078213443298 1109 54-55 0.4853439627780034 1109 56-57 0.7955328091930625 1109 58-59 -2.1811807876540428 1109 60-61 0.11503031584009449 1109 62-63 -0.9466270119809508 1109 64-65 0.1361598961070527 1109 66-67 0.9823483894699123 1109 68-69 -0.7239731914489482 1109 70-71 -1.0279237834881059 1109 72-73 0.49536201512655964 1109 74-75 -0.059196059819161206 1109 76 0.0796428836402363 1110 1 -1.4296623595156994 1110 2 -1.1258510357090081 1110 3 -0.5693378830071296 1110 4 -0.06575511299323722 1110 5 0.07441092666893923 1110 6 0.020223699819247543 1110 7 0.286317541206067 1110 8 1.0727251493434693 1110 9 1.4655853104345766 1110 10-11 -0.4378034923933143 1110 12-13 0.6822868258674077 1110 14-15 1.0402179698813399 1110 16-17 1.6096491998360136 1110 18-19 -0.2973187991513271 1110 20-21 2.0550623052408454 1110 22-23 0.17089809511868737 1110 24-25 -2.041358179386144 1110 26-27 1.60699881757197 1110 28-29 -0.17066785877534585 1110 30-31 -0.2821270621476373 1110 32-33 0.7942443724073129 1110 34-35 1.0729520295177508 1110 36-37 2.868404362661181 1110 38-39 1.2194082987810617 1110 40-41 -0.026613879043509314 1110 42-43 0.41062434597056097 1110 44-45 2.2804421162722477 1110 46-47 2.7853906998579845 1110 48-49 3.1384466443937455 1110 50-51 -0.5413638957323244 1110 52-53 0.4127125832490961 1110 54-55 0.6301852326192758 1110 56-57 1.749897888558145 1110 58-59 -1.4821067135799666 1110 60-61 1.6996864004961694 1110 62-63 0.6849867446328091 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-2.753041220919698 2116 68-69 -2.3327394252151876 2116 70-71 -2.3233783289426526 2116 72-73 -0.40398863422408837 2116 74-75 1.1372325116094117 2116 76 0.03418833818568956 2117 1 -0.40188458173792085 2117 2 0.7815563716984002 2117 3 0.6899213762521299 2117 4 0.2860967388586175 2117 5 0.5558924081504202 2117 6 0.5850385146340642 2117 7 0.10113235602088366 2117 8 -1.6957933691750497 2117 9 -1.1084887636394996 2117 10-11 -0.4100257146155286 2117 12-13 -2.725120581539997 2117 14-15 -0.9736709190075459 2117 16-17 -3.9088693186825054 2117 18-19 -2.4454669472994794 2117 20-21 -0.30604880587026884 2117 22-23 -2.676324127103534 2117 24-25 -1.9719137349417046 2117 26-27 -6.5735567379835835 2117 28-29 -3.8095567476642316 2117 30-31 -2.8700900251106027 2117 32-33 -2.5529778498149085 2117 34-35 2.7442483258140413 2117 36-37 -0.8260400817832618 2117 38-39 -0.26670281233005255 2117 40-41 -1.2580953605249903 2117 42-43 -1.6865978762516605 2117 44-45 -2.5065949207647904 2117 46-47 -5.709979670512382 2117 48-49 -3.0235903926432854 2117 50-51 -4.786734266102698 2117 52-53 0.0377125832490961 2117 54-55 -2.6753703229362813 2117 56-57 0.19434233300259507 2117 58-59 -0.26451412098737137 2117 60-61 -3.504017303207533 2117 62-63 -4.000198440552381 2117 64-65 -6.316221056273896 2117 66-67 -5.487889705768183 2117 68-69 -2.6584970009727584 2117 70-71 -5.944590450154772 2117 72-73 -0.9570189372543929 2117 74-75 -0.1961008217239204 2117 76 -2.8370237830264315 2118 1 -1.2185512484045873 2118 2 -0.35177696163493266 2118 3 0.38992137625213275 2118 4 0.15276340552528467 2118 5 0.5558924081504202 2118 6 0.7350385146340628 2118 7 1.1011323560208837 2118 8 1.1208732974916131 2118 9 -6.975155430306163 2118 10-11 -3.276692381282203 2118 12-13 -3.658453914873334 2118 14-15 0.9013290809924541 2118 16-17 -5.558869318682504 2118 18-19 -0.3954669472994823 2118 20-21 0.8106178607964054 2118 22-23 1.2153425395631317 2118 24-25 -0.2802470682750382 2118 26-27 -5.481890071316915 2118 28-29 1.3071099190024356 2118 30-31 -2.4617566917772713 2118 32-33 -0.47797784981490565 2118 34-35 -2.0307516741859573 2118 36-37 -4.97604008178326 2118 38-39 -2.7083694789967154 2118 40-41 -2.841428693858326 2118 42-43 -1.328264542918319 2118 44-45 -3.6232615874314575 2118 46-47 1.6650203294876178 2118 48-49 1.6680762740233774 2118 50-51 -4.895067599436032 2118 52-53 -5.020620750084241 2118 54-55 -1.1253703229362841 2118 56-57 -0.372324333664082 2118 58-59 -0.9561807876540414 2118 60-61 0.029316030125805526 2118 62-63 -2.65019844055238 2118 64-65 -0.8245543896072292 2118 66-67 1.2287769608984789 2118 68-69 -4.741830334306094 2118 70-71 0.8220762165118956 2118 72-73 2.759647729412272 2118 74-75 -2.312767488390591 2118 76 -2.3203571163597623 2119 1 1.4314487515954113 2119 2 -2.8017769616349355 2119 3 0.5649213762521299 2119 4 -0.5472365944747182 2119 5 -0.06910759184957982 2119 6 -0.5399614853659358 2119 7 0.22613235602088366 2119 8 -3.2791267025083854 2119 9 -1.0251554303061639 2119 10-11 -1.8266923812822 2119 12-13 1.4415460851266673 2119 14-15 -2.723670919007546 2119 16-17 -0.7213693186825054 2119 18-19 0.36703305270052056 2119 20-21 -4.139382139203597 2119 22-23 -1.8221574604368698 2119 24-25 -0.3052470682750368 2119 26-27 -0.09439007131691568 2119 28-29 -1.0178900809975673 2119 30-31 1.2757433082227294 2119 32-33 -3.5529778498149085 2119 34-35 -2.3182516741859587 2119 36-37 -2.326040081783262 2119 38-39 0.9416305210032831 2119 40-41 0.908571306141674 2119 42-43 -1.4782645429183248 2119 44-45 -4.485761587431455 2119 46-47 -1.5849796705123822 2119 48-49 -6.419423725976621 2119 50-51 0.7549324005639662 2119 52-53 -0.5456207500842396 2119 54-55 -4.237870322936281 2119 56-57 -3.5973243336640763 2119 58-59 -2.6186807876540428 2119 60-61 -2.9206839698741973 2119 62-63 -1.3751984405523814 2119 64-65 -0.712054389607232 2119 66-67 1.1787769608984817 2119 68-69 2.258169665693906 2119 70-71 -2.215423783488106 2119 72-73 -1.102852270587725 2119 74-75 3.6372325116094117 2119 76 -0.045357116359763694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 7.0 23 9.0 24 28.0 25 59.0 26 84.0 27 174.0 28 270.0 29 455.0 30 597.0 31 887.0 32 1174.0 33 1436.0 34 1715.0 35 989.0 36 154.0 37 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.7819287576020852 42.298622315998514 10.003723470274295 46.91572545612511 2 0.3847585950105498 4.319225518182947 88.6310040958173 6.665011790989202 3 0.3847585950105498 47.77212361921311 49.73315129700881 2.1099664887675313 4 0.33511232468660795 31.277150304083406 25.878118406354723 42.50961896487526 5 0.6826362169542013 66.72458731537793 27.640561002854657 4.952215464813206 6 0.3723470274295643 32.3073104133052 6.019610276777957 61.30073228248728 7 1.092217947126722 67.30793099168424 4.5178105994787146 27.082040461710317 8 0.4592280004964627 30.768276033263003 41.814571180340074 26.95792478590046 9 0.8439865955070125 26.75933970460469 4.579868437383642 67.81680526250466 10-11 0.7322824872781433 17.134169045550454 43.68251210127839 38.45103636589301 12-13 21.304455752761573 3.3883579496090355 26.386992677175126 48.92019362045426 14-15 32.903065657192506 23.209631376442843 5.783790492739233 38.103512473625415 16-17 26.374581109594143 22.055355591411196 0.856398163087998 50.71366513590666 18-19 16.6687352612635 26.641429812585333 0.4840511356584336 56.20578379049274 20-21 23.147573538537916 13.423110338835794 0.9184560009929253 62.51086012163336 22-23 32.896859873402015 37.29676058086136 0.90604443341194 28.900335112324687 24-25 26.455256298870548 45.34566215713045 1.3404492987464318 26.858632245252572 26-27 17.022464937321583 27.131686732034254 1.3900955690703736 54.455752761573784 28-29 25.139630135286083 7.093210872533201 21.18034007695172 46.586818915228996 30-31 21.769889537048527 1.929998758843242 54.38748913987836 21.91262256422986 32-33 27.863969219312402 1.9858508129576766 66.61908899093956 3.5310909767903684 34-35 21.83815315874395 23.824003971701625 52.20925902941541 2.12858384013901 36-37 5.994787141615986 22.756609159736875 47.58595010549833 23.662653593148814 38-39 21.378925158247487 7.614496710934591 27.336477597120517 43.6701005336974 40-41 12.59774109470026 22.229117537544994 38.66823879856025 26.50490256919449 42-43 34.721360307806876 12.610152662281246 45.92900583343676 6.739481196475115 44-45 14.65806131314385 14.74494228621075 27.18753878614869 43.40945761449671 46-47 36.89959041826983 2.9105125977410946 26.46766786645153 33.722229117537545 48-49 23.439245376691076 1.2659798932605186 33.02097554921187 42.27379918083654 50-51 5.814819411691697 1.5142112448802285 48.833312647387366 43.83765669604071 52-53 20.79558148194117 1.7438252451284597 59.544495469777836 17.91609780315254 54-55 12.423979148566463 1.5266228124612138 48.74643167432047 37.30296636465186 56-57 34.60965619957801 2.165818542881966 39.55566588060072 23.66885937693931 58-59 14.118158123370982 44.023830209755495 35.26126349757974 6.5967481692937815 60-61 14.453270448057589 75.40027305448677 7.707583467791983 2.4388730296636467 62-63 2.2961400024823138 94.65061437259526 0.9556907037358819 2.097554921186546 64-65 0.44061064912498443 96.51234950974307 0.6267841628397667 2.4202556782921683 66-67 0.42199329775350625 93.51495593893509 1.0425716768027802 5.020479086508626 68-69 1.42733027181333 80.81171651979645 3.493856274047412 14.267096934342808 70-71 2.7677795705597616 69.98262380538662 6.652600223408217 20.596996400645402 72-73 3.7669107608290924 60.3388357949609 9.110090604443341 26.784162839766662 74-75 4.157875139630135 57.41591162963883 9.3148814695296 29.11133176120144 76 3.140126597989326 60.54362666004717 7.620702494725083 28.695544247238423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 1.5 29 2.0 30 1.5 31 1.0 32 1.5 33 2.0 34 5.0 35 6.0 36 9.5 37 15.0 38 32.5 39 182.5 40 715.0 41 1451.5 42 1788.0 43 1832.5 44 1614.5 45 1049.5 46 747.0 47 559.5 48 283.0 49 142.0 50 90.0 51 68.0 52 38.0 53 22.0 54 14.0 55 12.0 56 9.0 57 6.0 58 4.0 59 3.0 60 2.0 61 3.0 62 4.0 63 2.0 64 0.0 65 0.5 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 8057.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.278763807868934 #Duplication Level Percentage of deduplicated Percentage of total 1 82.17270194986072 18.307062181953583 2 7.632311977715878 3.4007695171900214 3 2.896935933147632 1.9362045426337344 4 1.5041782729805013 1.3404492987464318 5 1.0027855153203342 1.1170410822886931 6 0.6685236768802229 0.8936328658309545 7 0.38997214484679665 0.6081668114682884 8 0.6128133704735376 1.092217947126722 9 0.1671309192200557 0.33511232468660795 >10 2.1727019498607243 9.122502172024326 >50 0.5013927576601671 6.962889412932853 >100 0.11142061281337048 5.746555789996276 >500 0.11142061281337048 20.80178726573166 >1k 0.05571030640668524 28.335608787389848 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT 2283 28.335608787389848 Illumina PCR Primer Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 918 11.393819039344669 No Hit CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 758 9.407968226386993 No Hit CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 279 3.462827355094948 No Hit TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT 184 2.283728434901328 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 75 0.9308675685739108 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 70 0.8688097306689836 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 68 0.8439865955070125 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT 65 0.8067518927640561 No Hit CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 64 0.7943403251830705 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 60 0.7446940548591287 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTCTT 55 0.6826362169542013 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT 53 0.6578130817922303 No Hit CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 51 0.6329899466302593 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT 47 0.5833436763063176 No Hit CTTATCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 35 0.4344048653344918 No Hit TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 34 0.42199329775350625 No Hit CTTATTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 33 0.4095817301725208 No Hit ACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCT 28 0.3475238922675934 Illumina PCR Primer Index 2 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT 27 0.33511232468660795 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT 26 0.32270075710562246 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTCTGCCGTCTT 26 0.32270075710562246 No Hit CTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 25 0.31028918952463697 No Hit CTTATACACATCTCCGAGCCCACGCGACCCGTTTGTATCTCGTATGCCGT 24 0.29787762194365147 No Hit CTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT 23 0.285466054362666 No Hit ATACACATCTCCGAGCCCACGAGACCCGATTGTATCTCGTATGCCGTCTT 22 0.2730544867816805 RNA PCR Primer, Index 35 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 22 0.2730544867816805 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTCTCTCGTATGCCGTCTT 22 0.2730544867816805 No Hit CTTATATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 20 0.24823135161970958 No Hit CTTATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGT 18 0.22340821645773862 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGTTTGTATCTCGTATGCCGT 18 0.22340821645773862 No Hit ATACACATCTCCGAGCCCACGAGAACCGTTTGTATCTCGTATGCCGTCTT 17 0.21099664887675312 TruSeq Adapter, Index 2 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCCGTCTGTATCTCGTATGCCGTCTT 17 0.21099664887675312 Illumina PCR Primer Index 2 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCAGTTTGTATCTCGTATGCCGTCTT 16 0.19858508129576763 TruSeq Adapter, Index 2 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCCGCTTGTATCTCGTATGCCGTCTT 16 0.19858508129576763 TruSeq Adapter, Index 8 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTC 15 0.18617351371478216 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTAGTATGCCGT 14 0.1737619461337967 No Hit CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTC 14 0.1737619461337967 No Hit ATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGTCTT 14 0.1737619461337967 TruSeq Adapter, Index 2 (95% over 21bp) TCTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 14 0.1737619461337967 No Hit TCTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 13 0.16135037855281123 No Hit CTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTC 12 0.14893881097182574 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTCGTATCTCGTATGCCGTCTT 12 0.14893881097182574 RNA PCR Primer, Index 27 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCAGTCTT 12 0.14893881097182574 No Hit ATACACATCTCCGCGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT 12 0.14893881097182574 Illumina PCR Primer Index 2 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCCT 12 0.14893881097182574 No Hit CTTATACACATCTCCGAGCCCAAGAGACCCGTTTGTATCTCGTATGCCGT 12 0.14893881097182574 No Hit ATACACATCTACGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT 12 0.14893881097182574 Illumina PCR Primer Index 2 (95% over 21bp) ATACACATCTCCGAGCCCACGAGCCCCGTTTGTATCTCGTATGCCGTCTT 11 0.13652724339084024 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTC 10 0.12411567580985479 No Hit CTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTGCTTGA 10 0.12411567580985479 Illumina PCR Primer Index 2 (96% over 28bp) TATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCT 10 0.12411567580985479 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCCCGTATGCCGT 10 0.12411567580985479 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTATT 9 0.11170410822886931 No Hit CTTATACCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 9 0.11170410822886931 No Hit ATTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 9 0.11170410822886931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.012411567580985479 0.0 27 0.0 0.0 0.0 0.012411567580985479 0.0 28 0.0 0.0 0.0 0.012411567580985479 0.0 29 0.0 0.0 0.0 0.012411567580985479 0.0 30 0.0 0.0 0.0 0.012411567580985479 0.0 31 0.0 0.0 0.0 0.012411567580985479 0.0 32 0.0 0.0 0.0 0.012411567580985479 0.0 33 0.0 0.0 0.0 0.012411567580985479 0.0 34 0.0 0.0 0.0 0.012411567580985479 0.0 35 0.0 0.0 0.0 0.012411567580985479 0.0 36 0.0 0.0 0.0 0.024823135161970957 0.0 37 0.0 0.0 0.0 0.09929254064788383 0.0 38 0.0 0.0 0.0 0.11170410822886931 0.0 39 0.0 0.0 0.0 0.11170410822886931 0.0 40 0.0 0.0 0.0 0.11170410822886931 0.0 41 0.0 0.0 0.0 0.12411567580985479 0.0 42 0.0 0.0 0.0 0.13652724339084027 0.0 43 0.0 0.0 0.0 0.13652724339084027 0.0 44 0.0 0.0 0.0 0.14893881097182574 0.0 45 0.0 0.0 0.0 0.1861735137147822 0.0 46 0.0 0.0 0.0 0.19858508129576766 0.0 47 0.0 0.0 0.0 0.24823135161970958 0.0 48 0.0 0.0 0.0 0.26064291920069504 0.0 49 0.0 0.0 0.0 0.29787762194365147 0.0 50 0.0 0.0 0.0 0.31028918952463697 0.0 51 0.0 0.0 0.0 0.31028918952463697 0.0 52 0.0 0.0 0.0 0.31028918952463697 0.0 53 0.0 0.0 0.0 0.3475238922675934 0.0 54 0.0 0.0 0.0 0.3475238922675934 0.0 55 0.0 0.0 0.0 0.3599354598485789 0.0 56 0.0 0.0 0.0 0.3723470274295644 0.0 57 0.0 0.0 0.0 0.3723470274295644 0.0 58 0.0 0.0 0.0 0.3723470274295644 0.0 59 0.0 0.0 0.0 0.3971701625915353 0.0 60 0.0 0.0 0.0 0.4095817301725208 0.0 61 0.0 0.0 0.0 0.42199329775350625 0.0 62 0.0 0.0 0.0 0.43440486533449174 0.0 63 0.0 0.0 0.0 0.44681643291547724 0.0 64 0.0 0.0 0.0 0.44681643291547724 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 15 0.0021452927 70.0 1 TCTTAAT 15 0.0021452927 70.0 1 CTTTACA 120 0.0 64.166664 1 TTTACAC 125 0.0 61.6 2 CTATACA 40 8.998541E-9 61.25 1 TTACACA 135 0.0 57.037037 3 TTTATAC 25 2.2507997E-4 55.999996 3 CAAACAC 25 2.2507997E-4 55.999996 69 CTTATAC 205 0.0 52.92683 1 CTTTATA 20 0.006705144 52.5 2 GACCCGA 20 0.006705144 52.5 23 TTATACA 220 0.0 49.31818 2 ACAAACA 30 5.5365666E-4 46.666664 68 TATACAC 290 0.0 38.62069 3 TCTTATA 50 1.5646413E-4 35.0 1 ATACACA 640 0.0 33.90625 1 GTATCTC 635 0.0 31.417324 32 AACAAAC 45 0.0040615974 31.111113 67 TATCTCG 635 0.0 30.866142 33 TGTATCT 645 0.0 30.387598 31 >>END_MODULE