FastQCFastQC Report
Wed 25 May 2016
SRR1780381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102998
Sequences flagged as poor quality0
Sequence length50
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG2100.20388745412532283No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC1750.16990621177110235No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG1060.10291461970135343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC1600.037.121722
TATATAC301.2972568E-436.6456223
CTTTACA1400.036.1396681
GGTATAC250.002343851435.196891
TTCTCGT250.002343851435.196899
ATATACA353.2083967E-431.4105344
TATCCAC353.2083967E-431.4105343
TTACACA1900.031.2452143
GGTGGTC1250.029.91735844
CTTAACA300.00572340529.3307421
TGCTTAA300.00572340529.33074241
TTATCCA300.00572340529.3307422
CTTATCA300.00572340529.3307421
CTTATAT300.00572340529.3307421
GACGAAT454.0363295E-529.33074223
TTATACC752.1191227E-929.330742
ACACTTC300.00573702229.3164986
ATAACAC300.00573702229.3164984
TATACCC852.582965E-1028.4542473
TCTCGTG554.888232E-628.01113710
Produced by FastQC (version 0.11.5)