SRR1780381_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780381_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102998
Sequences flagged as poor quality	0
Sequence length	50
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	36500	35.437581312258494	No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT	28767	27.92966853725315	RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	8231	7.991417309073963	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	4661	4.525330588943475	No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	4597	4.463193460067186	No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT	2428	2.3573273267442083	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	1618	1.5709042894036778	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	1326	1.2874036389056098	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	1116	1.083516184780287	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	957	0.9291442552282569	No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	815	0.7912775005339909	No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	715	0.6941882366647896	No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	596	0.5786520126604401	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	462	0.4485523990757102	No Hit
CTTATCACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC	448	0.434959902134022	No Hit
CTTATTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	429	0.41651294199887373	No Hit
ACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTTCT	335	0.32524903396182453	RNA PCR Primer, Index 20 (95% over 23bp)
ATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTTCTGCTT	287	0.2786461873046079	RNA PCR Primer, Index 34 (96% over 28bp)
CTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT	277	0.2689372609176877	No Hit
TCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	249	0.24175226703431135	No Hit
TCTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	217	0.21068370259616692	No Hit
CTTATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	209	0.2029165614866308	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT	187	0.18155692343540653	No Hit
CTTATACCACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	146	0.14175032524903397	No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT	128	0.12427425775257772	Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGTCTT	124	0.12039068719780968	No Hit
TCTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	111	0.10776908289481348	No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCATCTCGTATGCCGTCTTC	103	0.10000194178527738	TruSeq Adapter, Index 2 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACAC	40	8.11815E-9	44.04181	1
TCTTTAT	150	0.0	44.04181	1
TCTTAAC	20	7.7865506E-4	44.04181	1
CTTATAA	130	0.0	44.041805	1
TATACCA	35	1.4292164E-7	43.999027	3
ACCACAT	35	1.4292164E-7	43.999027	6
TATAACA	125	0.0	43.999027	3
TTATACC	35	1.4292164E-7	43.999027	2
TTATAAC	125	0.0	43.999027	2
ATAACAC	125	0.0	43.999027	4
CCACATC	35	1.4292164E-7	43.999027	7
TATCACA	35	1.4292164E-7	43.999027	3
ATCACAT	35	1.4292164E-7	43.999027	4
ATACCAC	35	1.4292164E-7	43.999027	4
TACCACA	35	1.4292164E-7	43.999027	5
CCGTCTT	3165	0.0	42.539345	44
CTTTACA	830	0.0	42.184624	1
TTTACAC	830	0.0	42.143646	2
TCTTAAT	115	0.0	42.12694	1
CTATACA	415	0.0	40.32744	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content