FastQCFastQC Report
Wed 25 May 2016
SRR1780380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45894
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCC252.3292664E-456.1811831
CGAAACA200.00691103352.4975472
ATCAGGG200.00691103352.4975472
CTATTTT400.002395919334.99836337
GGTGGGG552.8936635E-431.9211271
TGGTAAA450.004266846531.10965510
GACGAGG1107.3308236E-419.09001570
CGACGAG1452.3766127E-416.89576169
CCGACGA2100.003909080311.666121568
GCCGACG2200.00553144411.13584267
CATCTGA5401.7916584E-58.42553270
ACATCTG6057.423451E-57.520309469
ACACATC6551.9803592E-46.946240467
CACATCT6652.3852832E-46.84178568
TATACAC6157.407047E-46.828949564
TTATACA6208.1231195E-46.77387763
TCTTATA6258.900897E-46.71968661
CTTATAC6400.00116526116.562193462
ATACACA6450.00127269356.51132465
TACACAT6550.0015146566.411914366