##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780380_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45894 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.38974593628797 34.0 23.0 34.0 12.0 34.0 2 29.468863032204645 34.0 27.0 34.0 12.0 34.0 3 29.195820804462457 34.0 27.0 34.0 11.0 34.0 4 29.802566784329105 34.0 31.0 34.0 11.0 34.0 5 29.68825990325533 34.0 31.0 34.0 11.0 34.0 6 32.2994073299342 37.0 31.0 38.0 11.0 38.0 7 31.754194448076 37.0 27.0 38.0 11.0 38.0 8 31.359567699481413 37.0 27.0 38.0 11.0 38.0 9 31.898875670022225 37.0 28.0 38.0 12.0 38.0 10-11 31.957597943086242 37.0 31.0 38.0 11.0 38.0 12-13 32.07210092822591 37.0 31.0 38.0 11.0 38.0 14-15 31.86174663354687 37.0 29.0 38.0 11.0 38.0 16-17 31.652503595241207 37.0 27.0 38.0 11.0 38.0 18-19 31.71638994204035 37.0 29.0 38.0 11.0 38.0 20-21 31.781267703839283 37.0 28.0 38.0 11.0 38.0 22-23 31.572079138885258 37.0 27.0 38.0 11.0 38.0 24-25 31.926951235455615 37.0 31.0 38.0 11.0 38.0 26-27 31.88409160238811 37.0 30.5 38.0 11.0 38.0 28-29 31.921907003094084 37.0 31.0 38.0 11.0 38.0 30-31 31.873360352115746 37.0 30.5 38.0 11.0 38.0 32-33 31.563701137403584 37.0 27.0 38.0 11.0 38.0 34-35 31.723144637643266 37.0 27.0 38.0 11.0 38.0 36-37 31.663387370898157 37.0 27.0 38.0 11.0 38.0 38-39 31.907046672767684 37.0 29.0 38.0 11.0 38.0 40-41 31.606854926569923 37.0 27.0 38.0 11.0 38.0 42-43 31.856397350416174 37.0 28.5 38.0 11.0 38.0 44-45 31.05807948751471 37.0 26.5 38.0 11.0 38.0 46-47 31.67954416699351 37.0 27.0 38.0 11.0 38.0 48-49 31.659530657602303 37.0 27.0 38.0 11.0 38.0 50-51 31.456933368196278 37.0 26.5 38.0 11.0 38.0 52-53 31.437203120233583 37.0 27.0 38.0 11.0 38.0 54-55 31.286693249662264 37.0 27.0 38.0 11.0 38.0 56-57 31.22888612890574 37.0 26.5 38.0 11.0 38.0 58-59 31.142273499803895 37.0 26.5 38.0 11.0 38.0 60-61 31.282662221641175 37.0 26.5 38.0 11.0 38.0 62-63 31.194099446550748 37.0 26.5 38.0 11.0 38.0 64-65 31.35349936810912 37.0 27.0 38.0 11.0 38.0 66-67 31.325707064104243 37.0 26.5 38.0 11.0 38.0 68-69 30.72457183945614 37.0 25.0 38.0 11.0 38.0 70-71 30.91928138754521 37.0 25.0 38.0 11.0 38.0 72-73 31.086601734431518 37.0 26.0 38.0 11.0 38.0 74-75 30.639778620298948 36.5 25.0 38.0 11.0 38.0 76 29.95036388198893 35.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5439933733979316 1101 2 0.6888283823279338 1101 3 0.2345675532481124 1101 4 -1.5266062647636502 1101 5 -0.26058209464143545 1101 6 -0.7419410541050908 1101 7 -0.2862743607913565 1101 8 1.1965518284701346 1101 9 -1.2873331227942586 1101 10-11 -0.5481421038900542 1101 12-13 -0.4177565525592648 1101 14-15 -0.25972685413717755 1101 16-17 -1.4146691285705444 1101 18-19 -0.05231599286298305 1101 20-21 -0.2840774493144629 1101 22-23 -0.08726652800779888 1101 24-25 -0.12547257756772723 1101 26-27 0.016432163910394593 1101 28-29 -0.15576546310220962 1101 30-31 -1.0719587214936617 1101 32-33 0.12840059564713968 1101 34-35 -0.64162837023731 1101 36-37 -0.39494806246990777 1101 38-39 -0.553763710789422 1101 40-41 -0.1164284744339561 1101 42-43 -0.07746033919657691 1101 44-45 0.6306952936927246 1101 46-47 0.32814053612945315 1101 48-49 -0.9836044130294113 1101 50-51 -0.2325191106347937 1101 52-53 0.20224624064832142 1101 54-55 -0.6708032023851231 1101 56-57 0.056187547755300216 1101 58-59 -0.2589184415126269 1101 60-61 0.32841188759527284 1101 62-63 -1.404276121177027 1101 64-65 -0.6637373240514215 1101 66-67 -0.7741099456107534 1101 68-69 0.43362179341723106 1101 70-71 0.08983861576059482 1101 72-73 -0.5426960468495352 1101 74-75 -1.8116364128099782 1101 76 -0.7999756408385785 1102 1 0.47671174563264884 1102 2 -0.1776379122190832 1102 3 0.5086956959893953 1102 4 1.234903205204386 1102 5 1.3233595111979817 1102 6 1.065081511612192 1102 7 1.6716474940819772 1102 8 1.8871200265522887 1102 9 2.042660675211561 1102 10-11 1.0202229550288955 1102 12-13 -1.5138636085203352 1102 14-15 -0.08323241207811805 1102 16-17 -0.8578493203552853 1102 18-19 0.484084942206902 1102 20-21 0.9376438426086331 1102 22-23 0.5120130865144112 1102 24-25 1.1479638042842808 1102 26-27 -4.55410359297143 1102 28-29 0.6587949109257458 1102 30-31 0.8594376813497142 1102 32-33 -4.716358719271042 1102 34-35 0.7757419842305104 1102 36-37 1.0203708631538682 1102 38-39 1.6693411029122096 1102 40-41 1.5803417178278671 1102 42-43 1.6678046767854866 1102 44-45 -3.6671674036976682 1102 46-47 1.3311222641004434 1102 48-49 1.431356705237846 1102 50-51 -4.297640049521302 1102 52-53 0.26405149803107975 1102 54-55 1.30472277442896 1102 56-57 -0.11524988366495137 1102 58-59 1.7979967819976999 1102 60-61 0.8793159475160763 1102 62-63 -3.543487036686784 1102 64-65 0.6356281642363548 1102 66-67 1.4185812427867432 1102 68-69 -5.398924363731762 1102 70-71 0.2896143898171708 1102 72-73 1.2998925701057686 1102 74-75 -5.157493003621486 1102 76 -1.6374833933313049 1103 1 -0.759136265888344 1103 2 -0.09887420334570152 1103 3 -0.0035453586245424162 1103 4 0.5766557254215137 1103 5 0.7866363473561755 1103 6 -0.8793075434448596 1103 7 0.741438122197632 1103 8 1.3188249118962574 1103 9 0.5585574293934563 1103 10-11 1.288861483886766 1103 12-13 -1.2398523090853075 1103 14-15 -0.15939281974400643 1103 16-17 -0.11108959951779696 1103 18-19 0.2947086267089567 1103 20-21 0.25580048379617537 1103 22-23 0.5255834864257842 1103 24-25 0.904693614852583 1103 26-27 -0.10068606776926003 1103 28-29 1.6949087915062329 1103 30-31 1.0537990417130665 1103 32-33 -4.053070805235372 1103 34-35 -0.7749946946218209 1103 36-37 0.3712072429035054 1103 38-39 1.7066181606279578 1103 40-41 1.4796992009340961 1103 42-43 1.5373072800866936 1103 44-45 -3.703669009989902 1103 46-47 0.08175924205546892 1103 48-49 0.04872681712441107 1103 50-51 0.7708212332969104 1103 52-53 0.9276163590037179 1103 54-55 0.8921272277357062 1103 56-57 1.4809060928499491 1103 58-59 1.163511349406587 1103 60-61 2.045761056633168 1103 62-63 0.964378256323073 1103 64-65 1.2685073045930544 1103 66-67 1.56602779202791 1103 68-69 -2.373999139404745 1103 70-71 0.49754615009190317 1103 72-73 1.768265229904145 1103 74-75 -0.04067678560885568 1103 76 1.551172860130535 1104 1 -1.7194855211528548 1104 2 0.4252141376917926 1104 3 0.26425125154495177 1104 4 -0.4211871097094573 1104 5 0.032450420288892445 1104 6 -0.8929695876153758 1104 7 0.1272624673440106 1104 8 0.6913981014185993 1104 9 -0.7442674293636031 1104 10-11 -0.7387194097958236 1104 12-13 -1.1593181539748798 1104 14-15 -0.555900267098913 1104 16-17 -0.8057994094818497 1104 18-19 -0.8311259906510848 1104 20-21 -0.4523145649968363 1104 22-23 -0.570102183836152 1104 24-25 0.14778555116485137 1104 26-27 0.2769718675157975 1104 28-29 -0.8703197652477499 1104 30-31 -0.6438897102129708 1104 32-33 -1.0560497471973633 1104 34-35 -0.07268344654785963 1104 36-37 0.06309219411049227 1104 38-39 -0.3394527176463136 1104 40-41 0.38242133755454333 1104 42-43 0.006901527647045214 1104 44-45 -2.6549273561635047 1104 46-47 -0.2814148719661027 1104 48-49 -0.5890235680836007 1104 50-51 0.4572619112630143 1104 52-53 0.13511507497701203 1104 54-55 -0.07566938243378729 1104 56-57 0.24238529161728195 1104 58-59 0.32586368633519314 1104 60-61 -0.3480756151696056 1104 62-63 -0.04229868255725222 1104 64-65 -0.5764336301783821 1104 66-67 0.08554499798579229 1104 68-69 -3.409797804016968 1104 70-71 -0.6837070599748643 1104 72-73 0.3753530573309547 1104 74-75 -0.9539279412328945 1104 76 0.7758261729194409 1105 1 -0.014353273824074364 1105 2 -0.27522376737039167 1105 3 -0.25174104132595687 1105 4 -0.6095563512578899 1105 5 0.8551514187124312 1105 6 0.3148076062889871 1105 7 1.0317290989782713 1105 8 0.051916581012225294 1105 9 0.46613719491385197 1105 10-11 0.6095727604616705 1105 12-13 -0.40596380119856335 1105 14-15 0.1904651208849728 1105 16-17 0.9744072817074994 1105 18-19 1.0822563880492062 1105 20-21 0.0855904971856809 1105 22-23 0.44410345638104687 1105 24-25 0.5176226504817194 1105 26-27 0.6889354338146205 1105 28-29 -0.5753469153616031 1105 30-31 0.5198517506383205 1105 32-33 0.47199369298480676 1105 34-35 0.9771851819334891 1105 36-37 0.6229625742120923 1105 38-39 0.12357057890299572 1105 40-41 0.5489653908038044 1105 42-43 0.8397794397157057 1105 44-45 1.60662457937687 1105 46-47 0.8449119803723093 1105 48-49 0.7294034769330437 1105 50-51 0.6393428361185052 1105 52-53 -0.11335225730535115 1105 54-55 -0.11112217626808274 1105 56-57 -0.3030167052298012 1105 58-59 0.7719663312817708 1105 60-61 0.7664102842873959 1105 62-63 0.4574385744054368 1105 64-65 -0.3642773856521977 1105 66-67 0.0847567688738664 1105 68-69 0.8824425182811595 1105 70-71 1.421617865581272 1105 72-73 0.9930619380456136 1105 74-75 -0.6393019121559966 1105 76 -0.3986001714491003 1106 1 -0.9744489485300747 1106 2 -0.5545141799675619 1106 3 -0.8621855300642451 1106 4 -1.0420857512977548 1106 5 -0.4904335922502945 1106 6 -0.8472015625370055 1106 7 -0.11537076149611636 1106 8 0.4264033260789972 1106 9 0.5522842947743278 1106 10-11 -0.08793571073626438 1106 12-13 -0.6322544131180372 1106 14-15 -0.7131625450508459 1106 16-17 -1.3398641952707706 1106 18-19 -0.45380101677438844 1106 20-21 -1.265376215989523 1106 22-23 -0.4590259037943589 1106 24-25 -0.40362510714235356 1106 26-27 -0.5259539423065682 1106 28-29 -1.69498016232194 1106 30-31 -0.6529806193038787 1106 32-33 -0.07590345670624643 1106 34-35 -0.13349849356980314 1106 36-37 0.13968571553160203 1106 38-39 -0.2550222056295901 1106 40-41 -0.7902014419647863 1106 42-43 -0.03771707214396969 1106 44-45 0.10630566369020755 1106 46-47 -0.08925186256171713 1106 48-49 -0.5853663058056497 1106 50-51 -0.5186001577025046 1106 52-53 -1.1254909856290496 1106 54-55 -0.7975084628935569 1106 56-57 -0.25322599364917764 1106 58-59 -1.4185459270399363 1106 60-61 -0.348180108377548 1106 62-63 0.06365743029541093 1106 64-65 0.07696239907971503 1106 66-67 -0.0896901117320752 1106 68-69 -0.12285945500965312 1106 70-71 -0.39614175171989885 1106 72-73 -1.0136751558351875 1106 74-75 -1.6510021314105323 1106 76 -0.48666076542956915 1107 1 0.31213185745247785 1107 2 0.4378829949389633 1107 3 0.7971829784525823 1107 4 0.4616533430105143 1107 5 1.4317932461377403 1107 6 1.0023206643013793 1107 7 0.9062328945072089 1107 8 1.341525885281321 1107 9 0.15814220919061128 1107 10-11 0.47984575997416457 1107 12-13 0.9369396043310658 1107 14-15 0.8417813685345337 1107 16-17 1.4140618537529122 1107 18-19 1.0510390330802046 1107 20-21 1.1836474649410995 1107 22-23 0.7212815328487672 1107 24-25 0.5325610166632089 1107 26-27 1.3807141092098476 1107 28-29 0.7795524508895291 1107 30-31 0.7524410674431117 1107 32-33 0.7198173575854021 1107 34-35 0.7218401021925551 1107 36-37 1.0098975975424018 1107 38-39 1.2411971545698286 1107 40-41 0.5923702240094535 1107 42-43 0.9855068580596011 1107 44-45 0.8083621655597497 1107 46-47 1.4082346351532848 1107 48-49 0.8986179069072762 1107 50-51 1.565695646202773 1107 52-53 1.5443885324432394 1107 54-55 1.3805555171646091 1107 56-57 1.3479165073894563 1107 58-59 1.2619900098109191 1107 60-61 1.0343084666120674 1107 62-63 0.2208448004657484 1107 64-65 1.3427704589052247 1107 66-67 1.200276651874077 1107 68-69 1.864718442788437 1107 70-71 0.3838540937308679 1107 72-73 1.167795554592729 1107 74-75 1.3152213833381161 1107 76 0.37283237956420123 1108 1 0.4064186821707061 1108 2 0.9540508923349975 1108 3 0.905111466598715 1108 4 0.8722635256766615 1108 5 0.7749724144237895 1108 6 0.2994057789535347 1108 7 0.24925660224137047 1108 8 0.9029328032758812 1108 9 0.30734547386220257 1108 10-11 0.7366471591191335 1108 12-13 1.2259213377162226 1108 14-15 1.0044125383751847 1108 16-17 0.42665415845559096 1108 18-19 0.666332464871882 1108 20-21 1.1818985337323902 1108 22-23 0.5479818826350105 1108 24-25 -0.2730355632046475 1108 26-27 0.45624850485694424 1108 28-29 1.1536294038627055 1108 30-31 1.1212129290832102 1108 32-33 1.1217606144839714 1108 34-35 0.08919338629671358 1108 36-37 0.7844020670332661 1108 38-39 0.9355228444260284 1108 40-41 0.9694203600374358 1108 42-43 0.7263541180673698 1108 44-45 0.3091977660261307 1108 46-47 1.2971775034004622 1108 48-49 0.6110741836270535 1108 50-51 0.8411328790049488 1108 52-53 -0.0222651791774382 1108 54-55 0.24476072509309432 1108 56-57 0.9605671097991006 1108 58-59 -0.890069842501557 1108 60-61 1.2567142186524833 1108 62-63 1.6685517573254458 1108 64-65 0.7178967706036268 1108 66-67 0.6632576079564672 1108 68-69 1.6534475527767754 1108 70-71 1.5378960680798777 1108 72-73 1.1788721189145406 1108 74-75 2.221634131103375 1108 76 2.272404863046514 1109 1 -0.013484332798725518 1109 2 0.09033594399006972 1109 3 -0.32136966348772944 1109 4 0.5028770614135354 1109 5 -0.009973822135350474 1109 6 1.0846893547494503 1109 7 1.3709938993107365 1109 8 1.73025728145425 1109 9 0.513248910505677 1109 10-11 0.8764321053556863 1109 12-13 0.8227376986692079 1109 14-15 0.5965061500133899 1109 16-17 0.8905166927166093 1109 18-19 0.6736274258670356 1109 20-21 0.5748987445694134 1109 22-23 0.5120130865144112 1109 24-25 1.0303167454607518 1109 26-27 0.3903408514730131 1109 28-29 0.711082492625092 1109 30-31 0.11433964213402703 1109 32-33 0.8391968362845112 1109 34-35 0.07966355285796212 1109 36-37 0.8667760919120404 1109 38-39 0.5614979656573134 1109 40-41 0.2666162276317898 1109 42-43 0.6220530427985622 1109 44-45 0.37531625643305233 1109 46-47 -0.23096923916752843 1109 48-49 0.4182848098130165 1109 50-51 1.0618370746617067 1109 52-53 0.9437900601225842 1109 54-55 0.07596460449431675 1109 56-57 0.9991292013023667 1109 58-59 0.3339444944160057 1109 60-61 0.5002309801958162 1109 62-63 0.4761208064504707 1109 64-65 0.04412489626249538 1109 66-67 0.5002805891919735 1109 68-69 1.0945396885558196 1109 70-71 0.168699357137438 1109 72-73 0.03518668775281952 1109 74-75 1.6137488264438709 1109 76 -0.036176203788819805 1110 1 0.3714650807566535 1110 2 -0.06079665516831767 1110 3 0.10763024697874002 1110 4 0.6097800966002147 1110 5 0.47404444270483026 1110 6 1.3348012716623288 1110 7 -0.09789319890754555 1110 8 1.4893225513978479 1110 9 -0.43741811429510946 1110 10-11 -0.9705998581146282 1110 12-13 0.641387533032173 1110 14-15 0.311990974080004 1110 16-17 -0.14444615086831192 1110 18-19 0.18703507203052894 1110 20-21 0.10710843209739451 1110 22-23 -0.07369288537206131 1110 24-25 0.633526522522267 1110 26-27 -0.8614050523679744 1110 28-29 0.6249064697045092 1110 30-31 0.6401700656276255 1110 32-33 1.1214512905776495 1110 34-35 0.8627461923064423 1110 36-37 -0.4178957676993882 1110 38-39 0.271028809958505 1110 40-41 0.6585940233162724 1110 42-43 0.8320987048990176 1110 44-45 0.8435990033217564 1110 46-47 0.6661567345096131 1110 48-49 0.777269499579333 1110 50-51 0.1593167057835636 1110 52-53 0.07793367190519618 1110 54-55 -0.10603467923743892 1110 56-57 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-0.046349936901165734 2116 48-49 -0.5236556027156354 2116 50-51 -0.010270556269450282 2116 52-53 0.8147687546306948 2116 54-55 0.040780834016430845 2116 56-57 -0.6355367863047974 2116 58-59 -0.4719718115003033 2116 60-61 -1.4502401173341077 2116 62-63 0.2564026161440438 2116 64-65 -0.11193146567621781 2116 66-67 -0.1741952617544662 2116 68-69 0.05600306178389758 2116 70-71 -0.43608801235581396 2116 72-73 -1.0856859037080042 2116 74-75 -1.1387764260813782 2116 76 -0.939325342232074 2117 1 1.8828454811781477 2117 2 2.0667059791836344 2117 3 1.9463025335962314 2117 4 1.0841741956518476 2117 5 1.0925902804287517 2117 6 0.9405968459510561 2117 7 0.005584145665689988 2117 8 -0.2181590081385103 2117 9 0.8865018014056254 2117 10-11 1.7584833874069794 2117 12-13 1.9733158786591503 2117 14-15 1.4900204788218332 2117 16-17 1.245016441334002 2117 18-19 1.685191025665933 2117 20-21 1.1481516354693646 2117 22-23 1.2765178627838871 2117 24-25 1.8974359008152035 2117 26-27 2.213568604112531 2117 28-29 1.193083498155513 2117 30-31 1.7239424576191986 2117 32-33 2.299428108280491 2117 34-35 0.9336102597458442 2117 36-37 0.40201993303823613 2117 38-39 1.494579916386325 2117 40-41 0.9031672583004671 2117 42-43 1.3169248376703493 2117 44-45 1.5665178652817175 2117 46-47 2.1234802329289977 2117 48-49 1.6133074342474032 2117 50-51 2.080338834339937 2117 52-53 1.451432091294027 2117 54-55 1.6192024124380069 2117 56-57 2.1836440328760176 2117 58-59 1.4997564602279745 2117 60-61 2.8046050375110454 2117 62-63 3.001057960799386 2117 64-65 1.842214388469639 2117 66-67 0.3925380049788032 2117 68-69 1.298360704141544 2117 70-71 1.8391367628689572 2117 72-73 1.9361922181701203 2117 74-75 1.2479368606319063 2117 76 2.2327026297958987 2118 1 -1.5344927361600682 2118 2 -1.1701682576906052 2118 3 -0.31670112940743067 2118 4 0.4319153433929941 2118 5 -0.7718785840401132 2118 6 0.3283868337410425 2118 7 -0.45253551245397006 2118 8 -3.4032627932422947 2118 9 -0.10299758809376058 2118 10-11 -0.2108694819458954 2118 12-13 -0.26783186248858826 2118 14-15 -0.9137646249632709 2118 16-17 -0.06279796648040303 2118 18-19 -0.7488749084000048 2118 20-21 -0.8429350556173247 2118 22-23 -0.24008775382172232 2118 24-25 -0.20170939833009527 2118 26-27 0.37913636968029607 2118 28-29 -0.015341410269396505 2118 30-31 -1.4561552224784826 2118 32-33 -0.3494119405595555 2118 34-35 -0.17383784770226285 2118 36-37 -0.465623534605232 2118 38-39 -0.713478691672286 2118 40-41 -1.3771746220414158 2118 42-43 -0.735089814344299 2118 44-45 -0.5266445278806771 2118 46-47 -0.720231610782843 2118 48-49 -0.12466570372573216 2118 50-51 -0.4474999934988908 2118 52-53 -1.4969195570576197 2118 54-55 -0.9375741443385479 2118 56-57 0.3240591732911611 2118 58-59 0.45299211346362256 2118 60-61 -0.5231116902056812 2118 62-63 0.1712655310069593 2118 64-65 0.19398484024008766 2118 66-67 -1.622968710527914 2118 68-69 0.36624837202921157 2118 70-71 -0.4923650686328749 2118 72-73 -0.4283987464208465 2118 74-75 -0.7275210148259674 2118 76 -0.32749273039946303 2119 1 -1.4922772684126855 2119 2 0.060159334339115134 2119 3 0.624534939488214 2119 4 0.4707535737205859 2119 5 1.25952972396394 2119 6 0.6613824433323998 2119 7 -0.5423700278204002 2119 8 1.1019719247583808 2119 9 0.608924059998099 2119 10-11 0.4154391975542744 2119 12-13 -0.2018068709316836 2119 14-15 1.36350656720154 2119 16-17 0.7230851811048709 2119 18-19 0.5550764593811515 2119 20-21 0.19185048980651587 2119 22-23 0.5442756369246418 2119 24-25 0.9566830366580845 2119 26-27 0.6045669674513192 2119 28-29 -0.3106808226300828 2119 30-31 0.008721508355694851 2119 32-33 1.6102300722739464 2119 34-35 1.6373745805314392 2119 36-37 1.2859806531691653 2119 38-39 1.208000538317581 2119 40-41 0.28712797843139626 2119 42-43 0.06176935485529356 2119 44-45 0.05964388819497657 2119 46-47 0.8280628843201612 2119 48-49 0.1573336208267797 2119 50-51 1.946623613390674 2119 52-53 -0.2552782196716059 2119 54-55 -0.023187112930241227 2119 56-57 0.3137585991607992 2119 58-59 1.2760248726665928 2119 60-61 1.1064053648432903 2119 62-63 1.7246258822396499 2119 64-65 -0.43536335293789463 2119 66-67 1.3437655008871445 2119 68-69 0.5216012933395753 2119 70-71 -0.5004704384403702 2119 72-73 -0.026000907852797184 2119 74-75 0.5652854694698775 2119 76 2.240558603609319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 14.0 13 118.0 14 423.0 15 861.0 16 1248.0 17 1522.0 18 1657.0 19 1480.0 20 1183.0 21 915.0 22 658.0 23 510.0 24 437.0 25 378.0 26 390.0 27 460.0 28 593.0 29 642.0 30 744.0 31 1071.0 32 1373.0 33 1904.0 34 2888.0 35 4622.0 36 8725.0 37 11076.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.20533718943079 20.588493054642655 9.589851452609437 35.616318303317115 2 18.059005534492524 28.188870004793653 28.912276114524776 24.839848346189044 3 19.73896369895847 25.134004445025493 27.256286224778837 27.8707456312372 4 11.219331503028718 26.094914367891224 31.568396740314636 31.117357388765416 5 11.367499019479672 34.58622042097006 35.14620647579204 18.900074083758227 6 22.81561859938118 35.40332069551575 17.407504248921427 24.373556456181635 7 22.525820368675646 30.851527432779886 18.734475094783633 27.888177103760842 8 19.887131215409422 37.33385627750904 17.956595633416132 24.822416873665404 9 20.667189610842378 22.615156665359308 18.601560116790868 38.11609360700745 10-11 20.28478668235499 26.571011461193184 26.370549527171306 26.773652329280516 12-13 20.39046498452957 24.945526648363618 25.43469734605831 29.229311021048503 14-15 20.03203033076219 26.280123763454917 22.909312764195754 30.778533141587133 16-17 21.25441234148255 28.396958208044627 23.866954285963306 26.48167516450952 18-19 19.539591231969318 27.596199938989844 24.67969669237809 28.184512136662743 20-21 18.755174968405456 27.940471521331766 25.051204950538196 28.253148559724583 22-23 21.78716172048634 27.50904257637164 24.474876890225303 26.228918812916724 24-25 20.477622347147776 29.49296204296858 23.8429860112433 26.18642959864034 26-27 18.63533359480542 30.159715866997868 25.874842027280252 25.33010851091646 28-29 20.758704841591495 27.860940427942648 27.267180895106115 24.113173835359742 30-31 20.210702924129517 27.63868915326622 27.356517191789777 24.794090730814485 32-33 18.478450342092646 29.739181592365014 26.32370244476402 25.458665620778316 34-35 19.705190220943912 28.38497407068462 26.330239246960385 25.57959646141108 36-37 20.06471434174402 27.96988713121541 26.710463241382314 25.25493528565826 38-39 19.456791737482025 26.9664879940733 28.319606048720964 25.257114219723707 40-41 19.289013814441976 26.873883296291456 27.440406153309798 26.39669673595677 42-43 17.92500108946703 26.825946746851443 28.62574628491742 26.623305878764107 44-45 17.972937638907048 27.326012114873404 28.538588922299212 26.16246132392034 46-47 18.29650934762714 27.556979125811655 28.063581296029984 26.082930230531225 48-49 17.9315378916634 28.391510872880986 27.13426591711335 26.54268531834227 50-51 18.013247919117966 27.880550834531746 27.032945483069682 27.073255763280603 52-53 17.256068331372294 27.61254194448076 27.23667581818974 27.894713905957207 54-55 17.231010589619558 27.980781801542687 26.585174532618645 28.203033076219114 56-57 17.698391946659694 27.661567960953505 26.443543818364056 28.19649627402275 58-59 18.050289798230708 27.219244345666098 26.56447465899682 28.165991197106376 60-61 17.463067067590536 26.89458316991328 26.98827733472785 28.654072427768334 62-63 17.61777138623785 27.487253235717084 26.158103455789426 28.73687192225563 64-65 18.31503028718351 27.822809081797185 24.874711291236327 28.987449339782977 66-67 18.277988408070772 28.95040746067024 23.95520111561424 28.81640301564475 68-69 18.10803155096527 28.139843988320912 24.660086285788992 29.092038174924827 70-71 18.41417178716172 29.42541508693947 24.038000610101538 28.122412515797272 72-73 18.534013160761756 28.727066718961087 23.094522159759446 29.644397960517715 74-75 18.698522682703622 28.464505164073735 23.362531049810435 29.474441103412214 76 18.41417178716172 27.225781147862467 23.63053993986142 30.72950712511439 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.0 14 3.0 15 3.0 16 3.5 17 5.0 18 9.0 19 14.5 20 18.5 21 21.0 22 25.0 23 33.0 24 54.0 25 71.0 26 84.5 27 130.0 28 209.5 29 257.0 30 290.0 31 378.0 32 522.5 33 612.0 34 719.5 35 962.5 36 1207.5 37 1317.0 38 1514.5 39 1866.0 40 2246.0 41 2636.5 42 2801.0 43 2837.5 44 3048.0 45 3259.5 46 3297.0 47 3277.5 48 3202.0 49 3005.5 50 2865.0 51 2676.5 52 2309.0 53 1964.0 54 1798.0 55 1664.0 56 1390.5 57 1108.5 58 966.0 59 834.0 60 615.5 61 461.5 62 394.0 63 343.5 64 260.5 65 186.5 66 129.0 67 113.0 68 107.0 69 77.0 70 45.0 71 37.0 72 32.5 73 24.5 74 19.0 75 17.0 76 15.0 77 13.0 78 10.0 79 7.0 80 8.0 81 8.5 82 4.5 83 1.0 84 2.0 85 3.0 86 3.0 87 3.0 88 2.0 89 1.5 90 1.0 91 1.0 92 2.0 93 1.0 94 0.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5490913844947052 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 45894.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.86368588486512 #Duplication Level Percentage of deduplicated Percentage of total 1 83.53343756452992 61.700875931494316 2 8.746570695300747 12.921079008149214 3 3.492728399067819 7.739573800496798 4 1.7847134133750258 5.2730204384015344 5 0.9705301041328653 3.58434653767377 6 0.5427888728281071 2.405543208262518 7 0.3244933478863683 1.677779230400488 8 0.20059588778430043 1.1853401316076175 9 0.0973480043659105 0.6471434174401882 >10 0.30679371072893 2.865298296073561 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0021789340654551795 0.0 4 0.0 0.0 0.0 0.0021789340654551795 0.0 5 0.0 0.0 0.0 0.0021789340654551795 0.0 6 0.0 0.0 0.0 0.0021789340654551795 0.0 7 0.0 0.0 0.0 0.0021789340654551795 0.0 8 0.0 0.0 0.0 0.0021789340654551795 0.0 9 0.0 0.0 0.0 0.004357868130910359 0.0 10 0.0 0.0 0.0 0.006536802196365538 0.0 11 0.0 0.0 0.0 0.006536802196365538 0.0 12 0.0 0.0 0.0 0.006536802196365538 0.0 13 0.0 0.0 0.0 0.006536802196365538 0.0 14 0.0 0.0 0.0 0.006536802196365538 0.0 15 0.0 0.0 0.0 0.008715736261820718 0.0 16 0.0 0.0 0.0 0.008715736261820718 0.0 17 0.0 0.0 0.0 0.010894670327275896 0.0 18 0.0 0.0 0.0 0.013073604392731076 0.0 19 0.0 0.0 0.0 0.017431472523641436 0.0 20 0.0 0.0 0.0 0.019610406589096616 0.0 21 0.0 0.0 0.0 0.021789340654551792 0.0 22 0.0 0.0 0.0 0.026147208785462152 0.0 23 0.0 0.0 0.0 0.02832614285091733 0.0 24 0.0 0.0 0.0 0.03268401098182769 0.0 25 0.0 0.0 0.0 0.03268401098182769 0.0 26 0.0 0.0 0.0 0.03486294504728287 0.0 27 0.0 0.0 0.0 0.052294417570924304 0.0 28 0.0 0.0 0.0 0.06754695602911055 0.0 29 0.0 0.0 0.0 0.07844162635638646 0.0 30 0.0 0.0 0.0 0.10458883514184861 0.0 31 0.0 0.0 0.0 0.13945178018913149 0.0 32 0.0 0.0 0.0 0.17213579117095917 0.0 33 0.0 0.0 0.0 0.19610406589096613 0.0 34 0.0 0.0 0.0 0.2396827472000697 0.0 35 0.0 0.0 0.0 0.27890356037826297 0.0 36 0.0 0.0 0.0 0.36606092299647014 0.0 37 0.0 0.0 0.0 0.5011548350546913 0.0 38 0.0 0.0 0.0 0.6602170218329193 0.0 39 0.0 0.0 0.0 0.9412995162766374 0.0 40 0.0 0.0 0.0 1.2528870876367282 0.0 41 0.0 0.0 0.0 1.7562208567568744 0.0 42 0.0 0.0 0.0 2.3292805159715866 0.0 43 0.0 0.0 0.0 2.8870876367281126 0.0 44 0.0 0.0 0.0 3.438357955288273 0.0 45 0.0 0.0 0.0 3.924260251884778 0.0 46 0.0 0.0 0.0 4.397088944088551 0.0 47 0.0 0.0 0.0 4.891706976946877 0.0 48 0.0 0.0 0.0 5.310062317514272 0.0 49 0.0 0.0 0.0 5.721880855885301 0.0 50 0.0 0.0 0.0 6.118446855798144 0.0 51 0.0 0.0 0.0 6.538981130430993 0.0 52 0.0 0.0 0.0 7.142545866562078 0.0 53 0.0 0.0 0.0 8.007582690547784 0.0 54 0.0 0.0 0.0 8.615505294809779 0.0 55 0.0 0.0 0.0 9.107944393602649 0.0 56 0.0 0.0 0.0 9.643962173704624 0.0 57 0.0 0.0 0.0 10.027454569224735 0.0 58 0.0 0.0 0.0 10.419662701006667 0.0 59 0.0 0.0 0.0 10.892491393210442 0.0 60 0.0 0.0 0.0 11.28687845905783 0.0 61 0.0 0.0 0.0 11.7793175578507 0.0 62 0.0 0.0 0.0 12.252146250054473 0.0 63 0.0 0.0 0.0 12.82302697520373 0.0 64 0.0 0.0 0.0 13.37429729376389 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGATCC 25 2.3292664E-4 56.181183 1 CGAAACA 20 0.006911033 52.497547 2 ATCAGGG 20 0.006911033 52.497547 2 CTATTTT 40 0.0023959193 34.998363 37 GGTGGGG 55 2.8936635E-4 31.921127 1 TGGTAAA 45 0.0042668465 31.109655 10 GACGAGG 110 7.3308236E-4 19.090015 70 CGACGAG 145 2.3766127E-4 16.895761 69 CCGACGA 210 0.0039090803 11.6661215 68 GCCGACG 220 0.005531444 11.135842 67 CATCTGA 540 1.7916584E-5 8.425532 70 ACATCTG 605 7.423451E-5 7.5203094 69 ACACATC 655 1.9803592E-4 6.9462404 67 CACATCT 665 2.3852832E-4 6.841785 68 TATACAC 615 7.407047E-4 6.8289495 64 TTATACA 620 8.1231195E-4 6.773877 63 TCTTATA 625 8.900897E-4 6.719686 61 CTTATAC 640 0.0011652611 6.5621934 62 ATACACA 645 0.0012726935 6.511324 65 TACACAT 655 0.001514656 6.4119143 66 >>END_MODULE