FastQCFastQC Report
Wed 25 May 2016
SRR1780380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45894
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT26445.7611016690634935TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC9852.146250054473352RNA PCR Primer, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT9542.078703098444241No Hit
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC3650.7953109338911404TruSeq Adapter, Index 9 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT1840.400923868043753RNA PCR Primer, Index 9 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT1260.2745456922473526No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1020.2222512746764283No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1000.21789340654551795No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT800.17431472523641434No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT790.17213579117095917No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT790.17213579117095917No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG760.16559898897459363No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC720.1568832527127729TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT650.14163071425458665No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC620.1350939120582211No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC600.13073604392731075No Hit
TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC560.12202030766549005TruSeq Adapter, Index 9 (95% over 21bp)
CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG460.10023096701093825No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT460.10023096701093825No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG150.002211268370.01
GCTCTCG150.002211268370.036
TTAAACC150.002211268370.038
ATAACAC252.797702E-670.04
CTCTCGT150.002211268370.037
CTGCTCT200.006909756552.534
GCCCCAT200.006909756552.51
TTAATAC200.006909756552.52
TTTACAC1200.052.52
CTATACA407.3265255E-752.51
TGCTCTC200.006909756552.535
TATAACA407.3265255E-752.53
ACAAGCT200.006909756552.528
CTTTACA1250.050.3999981
CTTATAA352.0559788E-550.01
TTACACA1500.042.03
TTATAAC556.559687E-638.181822
TAACACA501.685054E-435.05
ATAGTAC400.002395370335.03
TTACTAC450.00426586931.1111134
Produced by FastQC (version 0.11.5)