FastQCFastQC Report
Wed 25 May 2016
SRR1780379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464171
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG301.2979863E-436.67583536
ATTGCCG250.002339730735.2354112
AACACGA353.2366105E-431.3924085
CCTTACG300.005734395229.34066834
GTGACGC454.096255E-529.299589
ATCCCAC407.019712E-427.503911
ATCACGT508.304184E-526.412324
GTATCAA609.717187E-625.67308434
TGACGCG450.00138788224.47167413
AATCGTT450.00139488224.45055639
GACGAAT450.00139575924.4479223
CACGTAG450.001403672724.42420826
CGTTGAA450.001405436324.41894542
TAACACG450.001406318924.4163154
TGTCGGA450.001406318924.4163157
GTACAAG651.8082737E-523.6956771
CGGTGGT1301.8189894E-1223.66504743
TTATACA6800.023.2698212
CGTGCGA952.6600901E-823.17118510
TATACAC7400.022.865553
Produced by FastQC (version 0.11.5)