FastQCFastQC Report
Wed 25 May 2016
SRR1780379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464171
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT331847.149089451947665No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT125782.7097772157243774No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC111882.4103186110291253No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC51051.0998101992584628No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT29100.6269241292540895No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG15380.33134340577071814No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC12590.2712362469865631No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC11100.23913600806599292No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11080.23870513237578395No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG10400.22405535890867803No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC9680.20854383406115418No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC6990.1505910537280442No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG6120.13184796120395284No Hit
CTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT5150.11095049022881653No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG4980.10728804686204008No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC4750.10233297642463662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATC207.853141E-443.99827611
GGCGGGT207.853141E-443.99827637
CCGTCTT35650.042.9492344
TTTACAC12350.039.5535282
GCCGTCT39650.038.5609143
CTTTACA12950.037.907191
CTTATAA1350.037.5042531
AGCGGAT601.9826984E-1036.6652335
CTATACA8150.035.383451
TAACGCT250.002349271235.2062074
TTACACA14500.033.6886983
CTTATAC19300.032.2786671
TATTGCG353.21557E-431.4273419
TTGCGCC353.21557E-431.42734111
TTACATT353.21557E-431.42734126
TTATACA20750.029.5859382
TTAAGAC300.00573646929.3385073
AACGCTC300.00573646929.3385075
GCTGAAC300.005742513629.33218643
CCGTCTA602.939778E-729.33218627
Produced by FastQC (version 0.11.5)