FastQCFastQC Report
Wed 25 May 2016
SRR1780378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14023
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT195613.94851315695643No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT181912.971546744633816TruSeq Adapter, Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC6294.485488126649076No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC3252.317621051130286No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG1921.3691792055908152No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1621.1552449547172503No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1300.9270484204521143No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC930.6631961777080511No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC860.6132781858375526No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT800.5704913356628396No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT750.5348356271839122No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG700.4991799187049847No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG680.4849176353134137No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT610.43499964344291525No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC590.4207373600513442No Hit
TTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC580.4136062183555588No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT500.3565570847892747No Hit
CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC500.3565570847892747No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTCTT390.2781145261356343No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG340.24245881765670685No Hit
CTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT340.24245881765670685No Hit
CTTATCACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC330.23532767596092133No Hit
CTTATTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG300.21393425087356485No Hit
CTTATACACATCTCCGAGCCCACGCGACGGCGTCGAATCTCGTATGCCGT280.19967196748199387No Hit
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTC280.19967196748199387No Hit
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTC270.19254082578620837No Hit
CTTATACACATCTCCGAGCACACGAGACGGCGTCGAATCTCGTATGCCGT240.1711474006988519No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGACTCTCGTATGCCGTCTT240.1711474006988519No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTAGAATCTCGTATGCCGT240.1711474006988519No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTAGTATGCCGT230.1640162590030664No Hit
TCTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT230.1640162590030664No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCCT220.15688511730728089No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTCTGCCGTCTT220.15688511730728089No Hit
CTTATACACATCTCCGAGCCCACGAGCCGGCGTCGAATCTCGTATGCCGT220.15688511730728089No Hit
ATACACATCTCCGAGCCCACGAGAAGGCGTCGAATCTCGTATGCCGTCTT200.1426228339157099RNA PCR Primer, Index 13 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCG190.1354916922199244No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGCATCTCGTATGCCGT190.1354916922199244No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGCATCTCGTATGCCGTCTT190.1354916922199244TruSeq Adapter, Index 18 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC190.1354916922199244No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCACGTATGCCGT180.12836055052413892No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCAGTCTT180.12836055052413892No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTC170.12122940882835342No Hit
ACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCT170.12122940882835342RNA PCR Primer, Index 8 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGCGTAGAATCTCGTATGCCGTCTT160.11409826713256793TruSeq Adapter, Index 8 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT160.11409826713256793RNA PCR Primer, Index 13 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCG150.10696712543678243No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAC150.002179155670.0000128
TCTTTAT150.002179155670.000011
CTTTACA900.066.111111
TTACACA900.066.111113
TTTACAC900.066.111112
CTATACA800.065.6251
TTTATAC252.310114E-456.0000043
CTTATAC4350.043.4482731
CTTAATA350.001213768440.01
TAATACA350.001213768440.03
AATACAC350.001213768440.04
TTAATAC350.001213768440.02
TTATACA4750.039.052632
TATACAC5650.032.831863
TCGAATA500.006973488328.00000230
CGAATAT500.006973488328.00000231
AAAACCA500.006973488328.00000267
AAAAACC808.085939E-526.2566
TCGAATC7400.023.6486533
CGAATCT7300.023.4931534
Produced by FastQC (version 0.11.5)