##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780378_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14023 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66676174855594 34.0 34.0 34.0 31.0 34.0 2 33.29758254296513 34.0 34.0 34.0 32.0 34.0 3 33.44512586465093 34.0 34.0 34.0 34.0 34.0 4 33.442273407972614 34.0 34.0 34.0 34.0 34.0 5 33.454325037438494 34.0 34.0 34.0 34.0 34.0 6 37.256435855380445 38.0 37.0 38.0 36.0 38.0 7 36.8170862155031 38.0 37.0 38.0 35.0 38.0 8 36.77822149326107 38.0 37.0 38.0 34.0 38.0 9 36.46502174998217 38.0 37.0 38.0 34.0 38.0 10-11 36.00085573700349 38.0 36.5 38.0 32.5 38.0 12-13 35.84439848819796 38.0 36.0 38.0 31.5 38.0 14-15 35.97104756471511 38.0 36.0 38.0 32.0 38.0 16-17 35.97044141767097 38.0 36.0 38.0 32.5 38.0 18-19 36.0604007701633 38.0 36.5 38.0 32.0 38.0 20-21 35.73279612065892 38.0 36.0 38.0 31.0 38.0 22-23 35.74006988518862 38.0 35.5 38.0 32.5 38.0 24-25 34.458960279540754 37.0 34.0 38.0 26.0 38.0 26-27 34.630285958782004 37.0 34.0 38.0 26.5 38.0 28-29 34.59776795264922 37.0 34.0 38.0 26.0 38.0 30-31 34.12322612850318 37.0 34.0 38.0 24.5 38.0 32-33 34.62946587748698 37.0 34.0 38.0 26.0 38.0 34-35 33.62532981530343 37.0 32.5 38.0 23.0 38.0 36-37 33.34728660058475 37.0 33.5 38.0 22.5 38.0 38-39 31.942558653640447 36.5 31.0 38.0 11.0 38.0 40-41 33.13962775440348 37.0 32.5 38.0 22.5 38.0 42-43 34.51586679027312 37.0 34.0 38.0 27.0 38.0 44-45 34.94566070027811 37.0 35.0 38.0 29.0 38.0 46-47 35.02374670184696 37.0 35.0 38.0 27.0 38.0 48-49 34.505883191899024 37.0 34.5 38.0 25.5 38.0 50-51 34.421450474220926 37.0 34.0 38.0 26.0 38.0 52-53 34.203059259787494 37.0 34.0 38.0 24.0 38.0 54-55 34.630250303073524 37.0 34.5 38.0 26.0 38.0 56-57 34.58810525565143 37.0 34.0 38.0 27.0 38.0 58-59 34.38001854096841 37.0 34.0 38.0 25.0 38.0 60-61 34.40690294516152 37.0 34.0 38.0 25.5 38.0 62-63 35.17503387292305 37.0 35.5 38.0 29.0 38.0 64-65 35.09416672609285 37.0 35.5 38.0 29.0 38.0 66-67 33.26502888112387 37.0 32.5 38.0 22.0 38.0 68-69 29.933430792269842 35.0 24.0 38.0 11.0 38.0 70-71 27.20309491549597 31.0 16.5 37.0 11.0 38.0 72-73 24.710618269985027 25.0 11.0 37.0 11.0 38.0 74-75 24.448691435498823 25.0 11.0 37.0 11.0 38.0 76 24.395493118448265 25.0 11.0 36.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.147781060170999 1101 2 0.6599136509879031 1101 3 0.4689883468194722 1101 4 0.42987484171175083 1101 5 0.170502518111455 1101 6 -1.806430358986006 1101 7 0.526755460147811 1101 8 0.4633041424975204 1101 9 -0.03665314008866005 1101 10-11 0.07461774972472313 1101 12-13 1.1458747262785707 1101 14-15 1.305550454442539 1101 16-17 -0.1717709588603995 1101 18-19 0.43616533613941044 1101 20-21 1.1385953902273087 1101 22-23 0.3886917709640727 1101 24-25 -2.098428450944482 1101 26-27 0.4275644237917575 1101 28-29 1.3628814158466085 1101 30-31 1.9904821151750056 1101 32-33 -0.08348078940230863 1101 34-35 1.9323392612316184 1101 36-37 0.3619640059128102 1101 38-39 -1.8911549416806182 1101 40-41 1.173476217906824 1101 42-43 1.0689884992787384 1101 44-45 1.0711130861624056 1101 46-47 1.7073941711486427 1101 48-49 -1.36029063395425 1101 50-51 -1.3386351120834306 1101 52-53 -1.7259807294119227 1101 54-55 -0.5945471567447669 1101 56-57 0.546545921587672 1101 58-59 -1.104538013763083 1101 60-61 -0.7111695564045846 1101 62-63 -0.6672424317673702 1101 64-65 -0.9372146259340894 1101 66-67 -0.8276065741506002 1101 68-69 0.46941958850571197 1101 70-71 -0.850078701347968 1101 72-73 -1.3468134935393614 1101 74-75 -1.5539910749056318 1101 76 -0.5917022529462841 1102 1 -0.07898228854357114 1102 2 0.2762617063603443 1102 3 0.2501353474786683 1102 4 0.5762163051263869 1102 5 0.43879520103828185 1102 6 0.565356061580907 1102 7 -0.9715965503993189 1102 8 -0.17281978630933992 1102 9 0.6924173938599267 1102 10-11 0.34488801999499685 1102 12-13 0.7744179036022345 1102 14-15 -0.3167303629602358 1102 16-17 -0.18198849346817525 1102 18-19 -0.8018043408546589 1102 20-21 1.0726758121126068 1102 22-23 0.5399772027373118 1102 24-25 -5.216094897879216 1102 26-27 -1.1643933876782526 1102 28-29 -0.19183183398859427 1102 30-31 1.4677398607254375 1102 32-33 0.8535660134981455 1102 34-35 1.8489509949165281 1102 36-37 -4.083981940033134 1102 38-39 -0.32556496145649305 1102 40-41 0.11579658705646523 1102 42-43 -0.8430088639381381 1102 44-45 1.3347913986212063 1102 46-47 1.355054026125572 1102 48-49 -4.54288786533197 1102 50-51 -0.4025771028546856 1102 52-53 -2.7160927926947203 1102 54-55 -1.83152804006712 1102 56-57 0.01094934940572756 1102 58-59 -0.7202268733543775 1102 60-61 -7.313674500372937 1102 62-63 -3.326767810804945 1102 64-65 -0.23912629369941385 1102 66-67 1.8451027205099209 1102 68-69 -3.402696429951771 1102 70-71 -2.59694752138752 1102 72-73 -2.625653308964541 1102 74-75 -2.0619014242793945 1102 76 0.29227928957184446 1103 1 1.0548658453045618 1103 2 0.3277417578403998 1103 3 -0.22090712356380493 1103 4 0.1952639241740073 1103 5 0.24831901056209205 1103 6 0.08787858410342864 1103 7 1.017463938661166 1103 8 0.8831647696752185 1103 9 1.052777754220287 1103 10-11 -0.532525107418131 1103 12-13 0.12351700270133392 1103 14-15 -0.33120912743899567 1103 16-17 -0.15463971611939797 1103 18-19 0.14928961023929332 1103 20-21 -0.13260089316409562 1103 22-23 0.7095396222997294 1103 24-25 -2.3850138167981356 1103 26-27 -0.5015877248725928 1103 28-29 1.0713599292031688 1103 30-31 1.8020384450240243 1103 32-33 1.2547886647207989 1103 34-35 0.9358235817891156 1103 36-37 -0.899940755991949 1103 38-39 0.5080901221985883 1103 40-41 0.5343937556536247 1103 42-43 0.29663077570150875 1103 44-45 1.4892315530613658 1103 46-47 0.9010643221358663 1103 48-49 -2.8617424341865387 1103 50-51 -0.18764788792547193 1103 52-53 0.5754129988110677 1103 54-55 0.4249069663678853 1103 56-57 0.1139094523658386 1103 58-59 -0.19513034825785525 1103 60-61 -3.4713321580305987 1103 62-63 -0.524966009003144 1103 64-65 -0.18024598481910203 1103 66-67 -1.5680246926174988 1103 68-69 0.04839752114217788 1103 70-71 1.177505503065504 1103 72-73 -2.24373567704691 1103 74-75 -0.7308203431983138 1103 76 -2.947102949810393 1104 1 0.24534203578075164 1104 2 0.45288018530440866 1104 3 0.49337859072191037 1104 4 0.18086746791708208 1104 5 0.22949287545688435 1104 6 0.33594059960730505 1104 7 0.7411627204994815 1104 8 0.6090783909376754 1104 9 1.3473513976311366 1104 10-11 -1.6811962037636476 1104 12-13 0.47429219649978194 1104 14-15 0.9918805735576797 1104 16-17 0.3500668176569022 1104 18-19 0.42226856926476586 1104 20-21 -0.053974093607067175 1104 22-23 1.0270922247360588 1104 24-25 1.2835908343646594 1104 26-27 1.79852301709861 1104 28-29 2.584095255891981 1104 30-31 2.2735223874381916 1104 32-33 1.6819758186521412 1104 34-35 1.6484481665067179 1104 36-37 2.7921965640523467 1104 38-39 2.1562075086880697 1104 40-41 2.3901523381563976 1104 42-43 1.5485687601976323 1104 44-45 1.9227863703371142 1104 46-47 1.760145163774844 1104 48-49 -0.6538243832452366 1104 50-51 0.6772469072018836 1104 52-53 1.0720907396748558 1104 54-55 0.8620608977078632 1104 56-57 0.4339537491543197 1104 58-59 -1.955927690450551 1104 60-61 0.582931407860876 1104 62-63 0.38976267316739666 1104 64-65 0.5531427194998315 1104 66-67 -0.6693535962719821 1104 68-69 2.751609038307187 1104 70-71 1.2038067212271883 1104 72-73 1.3312920084458888 1104 74-75 -0.4691924362215687 1104 76 0.6907708043424279 1105 1 0.49534203578075164 1105 2 0.09505777515641256 1105 3 0.19792404526736362 1105 4 -1.5828746039645232 1105 5 -0.5612047989617182 1105 6 -0.1318183855935402 1105 7 0.6365115577087863 1105 8 -0.4707313342208863 1105 9 0.7194444208869513 1105 10-11 0.017799567906543245 1105 12-13 -0.8613314821471505 1105 14-15 0.3911934699635964 1105 16-17 -0.28180421828390223 1105 18-19 1.069203030152714 1105 20-21 0.20289694233373723 1105 22-23 0.3870287997889079 1105 24-25 0.9899861832018644 1105 26-27 -0.25591672920159425 1105 28-29 1.2212517040949464 1105 30-31 2.4849388779244492 1105 32-33 2.3965635564956926 1105 34-35 -1.262228366262839 1105 36-37 0.8392367331855368 1105 38-39 -1.2705895314810647 1105 40-41 0.7411037123635822 1105 42-43 0.3913277453984776 1105 44-45 1.8707250595548643 1105 46-47 1.5347223307938762 1105 48-49 -0.33035715280125544 1105 50-51 1.6051010298234445 1105 52-53 0.13710131049938212 1105 54-55 1.2338896503505765 1105 56-57 0.6258142142705978 1105 58-59 0.15876575563824247 1105 60-61 1.1915466298481903 1105 62-63 -1.6348144938516285 1105 64-65 1.116831937258823 1105 66-67 1.188468813876007 1105 68-69 -0.599870877126218 1105 70-71 1.6834578840178835 1105 72-73 -1.0970906554018889 1105 74-75 -1.7421839795619505 1105 76 -2.359440612148056 1106 1 -0.7290169385782193 1106 2 0.11895054904918823 1106 3 0.44209653943985927 1106 4 -0.03916831025822631 1106 5 -0.07402531178222915 1106 6 0.16480166102650173 1106 7 -1.017334596137367 1106 8 0.6359120224224739 1106 9 -1.6395299380874064 1106 10-11 0.5874382625452341 1106 12-13 1.7453118744962097 1106 14-15 -1.489634035863908 1106 16-17 0.2528694413897554 1106 18-19 1.2078976688473517 1106 20-21 0.9534796929164884 1106 22-23 1.4027630655231746 1106 24-25 0.8265246447403243 1106 26-27 1.774969051684188 1106 28-29 0.9394917973350374 1106 30-31 1.3578992508848273 1106 32-33 0.8674260273581638 1106 34-35 1.9504755964411302 1106 36-37 3.0927332366820366 1106 38-39 1.8002146643231285 1106 40-41 2.370474341734216 1106 42-43 1.7325282115989395 1106 44-45 1.9910630548928694 1106 46-47 1.9569251279966693 1106 48-49 2.937708115264016 1106 50-51 0.9259052256276377 1106 52-53 0.7915302149282866 1106 54-55 0.2866285780895055 1106 56-57 1.6578654963218824 1106 58-59 2.1803275272000207 1106 60-61 2.6312319445335106 1106 62-63 2.000675016637885 1106 64-65 -2.2983778529509706 1106 66-67 0.6004734758806691 1106 68-69 3.1326466053912654 1106 70-71 0.7315348070948069 1106 72-73 1.0465573632461265 1106 74-75 0.2627694003914307 1106 76 -0.36468536739281276 1107 1 0.7236029053459703 1107 2 -0.5304362959898299 1107 3 0.2977264168088638 1107 4 0.27186847903943345 1107 5 -0.01772653809215541 1107 6 -0.006324314447297752 1107 7 0.788685470752263 1107 8 -0.2938538638651522 1107 9 -2.237077318243486 1107 10-11 0.7376612279855905 1107 12-13 -0.6735844465740257 1107 14-15 0.5725175806355338 1107 16-17 0.689045584087637 1107 18-19 0.8982544135519248 1107 20-21 0.11791670517958863 1107 22-23 0.049084135757290426 1107 24-25 0.45194270494098987 1107 26-27 0.5118698320632262 1107 28-29 2.216805063778736 1107 30-31 1.173674055790066 1107 32-33 1.6564449794205913 1107 34-35 -0.030014927527659552 1107 36-37 2.122833571130201 1107 38-39 0.49670295863751335 1107 40-41 0.10375193370745706 1107 42-43 2.108224978600056 1107 44-45 0.6973060872228487 1107 46-47 0.7328448604381421 1107 48-49 0.43742940846356504 1107 50-51 1.78543699820289 1107 52-53 1.7586428124756708 1107 54-55 1.266004274856499 1107 56-57 1.723640301227121 1107 58-59 1.7644969809346946 1107 60-61 1.3402620448679556 1107 62-63 0.6561934112867078 1107 64-65 1.4315552574169175 1107 66-67 0.7813541893700844 1107 68-69 2.1451884114113327 1107 70-71 0.6888926666265789 1107 72-73 1.406367842621826 1107 74-75 1.1387448741929909 1107 76 0.2858163048479909 1108 1 -0.43465796421924807 1108 2 0.20869413879277943 1108 3 -0.8066214092780868 1108 4 -0.20378369487361425 1108 5 0.09879520103827844 1108 6 -0.16545474922990877 1108 7 -0.5034884422912143 1108 8 -0.38254951603906306 1108 9 -2.100555579113049 1108 10-11 -0.3653822502752746 1108 12-13 0.9668503360346747 1108 14-15 0.5816480154181392 1108 16-17 0.11774123626155131 1108 18-19 0.7873848483345327 1108 20-21 1.4083514877882806 1108 22-23 0.6043015270616294 1108 24-25 1.6849861832018647 1108 26-27 -0.41682582011068803 1108 28-29 -0.5510210231777819 1108 30-31 1.1158479688335419 1108 32-33 2.2138362837684227 1108 34-35 0.2986807246462533 1108 36-37 2.821964005912804 1108 38-39 0.9271377412462058 1108 40-41 0.9920128032726723 1108 42-43 -0.8600358909651575 1108 44-45 0.5320886959185103 1108 46-47 1.7015405126120555 1108 48-49 2.288733756289652 1108 50-51 1.4428283025507156 1108 52-53 1.3825558559539317 1108 54-55 0.13252601398693287 1108 56-57 0.4458142142705981 1108 58-59 0.3705839374564306 1108 60-61 1.5620011753027399 1108 62-63 1.5083673243301945 1108 64-65 1.215468300895182 1108 66-67 0.9548324502396426 1108 68-69 3.9564927592374204 1108 70-71 -1.0415421159821179 1108 72-73 -1.1975452008564353 1108 74-75 0.07917965680168848 1108 76 0.6814684787610332 1109 1 -1.3796579642192484 1109 2 0.35452747212611513 1109 3 0.28504525738857467 1109 4 0.5137163051263869 1109 5 0.042961867704946144 1109 6 0.15037858410342864 1109 7 -0.05098844229121369 1109 8 0.7432838172942695 1109 9 0.5111110875536156 1109 10-11 0.4287844163913874 1109 12-13 0.029767002701333922 1109 14-15 1.2283146820848145 1109 16-17 1.2456579029282153 1109 18-19 0.9707181816678698 1109 20-21 1.4462681544549554 1109 22-23 0.8851348603949702 1109 24-25 -0.6870971501314642 1109 26-27 0.038590846555976555 1109 28-29 -1.683104356511116 1109 30-31 0.5445979688335427 1109 32-33 1.7488362837684193 1109 34-35 -0.02548594202040988 1109 36-37 2.1223806725794816 1109 38-39 1.8146377412462087 1109 40-41 1.5090961366060043 1109 42-43 1.838297442368166 1109 44-45 1.1841720292518332 1109 46-47 1.1436238459453847 1109 48-49 0.6412337562896511 1109 50-51 0.07574496921738927 1109 52-53 -0.16119414404607113 1109 54-55 -0.787890652679728 1109 56-57 -0.9991857857294022 1109 58-59 -0.003166062543570547 1109 60-61 1.8828345086360656 1109 62-63 1.2771173243301917 1109 64-65 2.209634967561854 1109 66-67 1.2623324502396471 1109 68-69 1.1198260925707508 1109 70-71 1.6522078840178835 1109 72-73 0.5032881324768965 1109 74-75 -0.4704036765316495 1109 76 -2.372698187905634 1110 1 0.36438965482837204 1110 2 0.589646519745159 1110 3 0.39814049548381547 1110 4 0.4095496384597226 1110 5 0.36736662960971245 1110 6 0.46883096505580824 1110 7 0.8269877481849761 1110 8 0.8117361982466491 1110 9 1.457539658982192 1110 10-11 0.3008082259151976 1110 12-13 0.21875509793942882 1110 14-15 0.9664099201800482 1110 16-17 0.6786936172139306 1110 18-19 -0.3269008659511812 1110 20-21 1.4150181544549554 1110 22-23 -1.9333175205574165 1110 24-25 -2.646918578702895 1110 26-27 1.343650370365502 1110 28-29 1.5951694530126943 1110 30-31 -0.6265329835474063 1110 32-33 1.3262172361493683 1110 34-35 0.05487120083672892 1110 36-37 2.2667259106747153 1110 38-39 2.389042503150968 1110 40-41 0.08201280327267568 1110 42-43 0.7490117280824578 1110 44-45 1.3225648863946944 1110 46-47 1.4486833697549173 1110 48-49 2.6620670896229797 1110 50-51 0.9194949692173822 1110 52-53 1.28969871309679 1110 54-55 0.18681172827265158 1110 56-57 1.0662904047467876 1110 58-59 -1.8022732054007164 1110 60-61 -0.0070464437448833905 1110 62-63 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66-67 -1.8001675497603529 2116 68-69 1.0208677592374187 2116 70-71 1.5740828840178835 2116 72-73 -0.6269202008564356 2116 74-75 -0.6058203431983138 2116 76 -2.143531521238966 2117 1 1.1828420357807516 2117 2 0.7086941387927794 2117 3 0.49337859072191037 2117 4 -0.5175336948736131 2117 5 0.37629520103828185 2117 6 0.41079525077009293 2117 7 0.9802615577087863 2117 8 0.7537004839609338 2117 9 -1.0930555791130487 2117 10-11 0.058992749724723126 2117 12-13 -1.1525246639653304 2117 14-15 0.5772730154181431 2117 16-17 -0.20225876373844898 2117 18-19 -2.768865151665466 2117 20-21 -0.18914851221171602 2117 22-23 -0.18257347293836546 2117 24-25 -4.260013816798136 2117 26-27 -3.5499508201106877 2117 28-29 -2.28727102317778 2117 30-31 -0.17415203116645728 2117 32-33 -3.0636637162315807 2117 34-35 0.8443057246462544 2117 36-37 -2.71616099408719 2117 38-39 0.6115127412462087 2117 40-41 0.14451280327267568 2117 42-43 1.1612141090348373 2117 44-45 -0.4147863040814954 2117 46-47 -0.6428344873879439 2117 48-49 -1.233766243710349 2117 50-51 -0.6065466974492821 2117 52-53 -0.12994414404607113 2117 54-55 -0.4024739860130637 2117 56-57 1.0633142142705978 2117 58-59 -1.0656660625435705 2117 60-61 -0.747373824697263 2117 62-63 -0.9572576756698083 2117 64-65 -2.4414066991048173 2117 66-67 -0.48766754976035287 2117 68-69 0.8958677592374187 2117 70-71 0.7459578840178835 2117 72-73 1.0293297991435644 2117 74-75 3.800429656801686 2117 76 3.231468478761034 2118 1 0.8120087024474216 2118 2 0.5086941387927766 2118 3 -0.6732880759447539 2118 4 -0.7237836948736103 2118 5 0.1387952010382847 2118 6 0.1545452507700915 2118 7 0.8698448910421206 2118 8 -1.4025495160390662 2118 9 -1.9138889124463816 2118 10-11 -1.0087155836086055 2118 12-13 -2.759816330631999 2118 14-15 0.6616480154181374 2118 16-17 -3.518925430405112 2118 18-19 0.17071818166786557 2118 20-21 -0.8516485122117174 2118 22-23 -0.46903180627169405 2118 24-25 0.14831951653519582 2118 26-27 -1.8634924867773535 2118 28-29 -1.8143543565111173 2118 30-31 -2.0908186978331216 2118 32-33 -2.2594970495649136 2118 34-35 -1.0379859420204127 2118 36-37 -5.454702660753856 2118 38-39 -1.889528925420457 2118 40-41 -1.3679871967273236 2118 42-43 -0.5700358909651655 2118 44-45 -5.534577970748163 2118 46-47 -1.0417928207212768 2118 48-49 -3.0212662437103504 2118 50-51 -4.297171697449283 2118 52-53 -5.767444144046074 2118 54-55 -3.4274739860130623 2118 56-57 -1.074185785729405 2118 58-59 -4.142749395876905 2118 60-61 -3.271332158030596 2118 62-63 -4.758299342336475 2118 64-65 -1.227865032438146 2118 66-67 -1.0585008830936857 2118 68-69 -5.18017390742925 2118 70-71 -1.0248754493154522 2118 72-73 -0.30087853418976707 2118 74-75 -1.7474870098649795 2118 76 -1.9518648545722996 2119 1 0.949045739484454 2119 2 -0.5876021575035182 2119 3 -0.46958437224105154 2119 4 0.4651051940152726 2119 5 -0.931575169332092 2119 6 -0.09730660108176181 2119 7 -0.4190439978467708 2119 8 -0.30995692344647097 2119 9 -0.7250000235574916 2119 10-11 -0.6105674354604673 2119 12-13 -0.2931496639653304 2119 14-15 -1.456870503100376 2119 16-17 -0.9152217267014109 2119 18-19 -0.9089114479617635 2119 20-21 -1.4553522159154255 2119 22-23 0.06800523076533693 2119 24-25 0.1242454424611239 2119 26-27 0.6994704761856099 2119 28-29 -1.2328728750296278 2119 30-31 -0.20192980894423584 2119 32-33 -0.8020896421575046 2119 34-35 -2.0139118679463373 2119 36-37 -0.15655451260570885 2119 38-39 -4.6969363328278675 2119 40-41 -0.4087279374680648 2119 42-43 -0.6848507057799793 2119 44-45 0.835792399622207 2119 46-47 0.804503475575018 2119 48-49 -1.5138588363029442 2119 50-51 1.6324579321803512 2119 52-53 -0.7711478477497806 2119 54-55 0.33363712509805055 2119 56-57 -1.587148748692364 2119 58-59 -1.150156803284311 2119 60-61 -1.5639247506231868 2119 62-63 -2.8212623052994417 2119 64-65 -1.1074946620677792 2119 66-67 0.16742504283224235 2119 68-69 1.471677944422602 2119 70-71 -0.32117174561174267 2119 72-73 0.9083807250694882 2119 74-75 1.5376981753202053 2119 76 -1.2222352249426685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 11.0 24 31.0 25 65.0 26 146.0 27 247.0 28 354.0 29 575.0 30 849.0 31 1070.0 32 1563.0 33 2045.0 34 2305.0 35 1723.0 36 1324.0 37 1712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.775725593667547 27.690223204735076 10.611138843328817 46.92291235826856 2 7.195321971047565 9.684090422876702 69.56428724238751 13.556300363688226 3 6.7460600442130785 33.13128431861941 49.518647935534474 10.60400770163303 4 4.756471511088925 34.54325037438494 30.82079440918491 29.879483705341226 5 4.335734151037581 49.154959709049415 38.89324680881409 7.616059331098909 6 9.398844755045282 42.55152249875205 11.324253012907366 36.7253797332953 7 9.505811880482065 47.47201026884404 11.302859587820011 31.719318262853886 8 8.029665549454467 40.50488483206161 27.30514155316266 24.16030806532126 9 8.286386650502745 23.454325037438494 10.58974541824146 57.66954289381731 10-11 8.856877986165586 24.31719318262854 37.66312486629109 29.162803964914787 12-13 18.245026028667187 10.564786422306211 30.72095842544391 40.469229123582686 14-15 24.146045781929686 19.903016472937317 13.228267845682092 42.722669899450906 16-17 28.396206232617843 27.437067674534692 9.238394066890109 34.92833202595735 18-19 23.58981672965842 23.668259288312058 9.327533338087427 43.4143906439421 20-21 19.47514797119019 17.18248591599515 9.45945945945946 53.8829066533552 22-23 24.798545247094058 34.54325037438494 9.712614989659844 30.945589388861155 24-25 28.852599301148114 39.328246452257005 9.438066034372103 22.381088212222778 26-27 43.79947229551451 30.845753405120156 9.855237823575555 15.499536475789775 28-29 43.43578406902945 14.105398274263708 10.2260571917564 32.23276046495044 30-31 32.90308778435428 10.283106325322684 20.5020323753833 36.311773514939745 32-33 34.33644726520716 19.917278756328887 26.527847108322046 19.21842687014191 34-35 26.581330671040433 28.032518006132783 23.069243385866077 22.31690793696071 36-37 12.023104899094344 40.979105754831345 25.946659060115522 21.051130285958784 38-39 17.956214789987875 17.449903729587106 28.856164871996004 35.73771660842901 40-41 14.668758468230763 20.441417670969123 35.666405191471156 29.22341866932896 42-43 31.737146117093346 16.216216216216218 39.324680881409115 12.721956785281325 44-45 16.15560151180204 23.36875133708907 31.66583470013549 28.8098124509734 46-47 32.325465306995646 10.79654852741924 24.766455109463024 32.11153105612208 48-49 25.69350352991514 9.922983669685518 27.322969407402127 37.06054339299722 50-51 11.441916850887827 9.634172431006203 41.99885901732868 36.925051700777296 52-53 16.761748555943807 9.787491977465592 53.644013406546385 19.806746060044215 54-55 13.713185480995508 9.580688868287812 43.31812023104899 33.388005419667685 56-57 30.432147186764603 9.972901661556016 31.88690009270484 27.708051058974544 58-59 15.27490551237253 30.945589388861155 40.30877843542751 13.4707266633388 60-61 20.587606075732726 53.77593952791842 15.802609997860658 9.833844398488198 62-63 8.125935962347572 71.9781787064109 9.826713256792413 10.069172074449119 64-65 6.920773015759823 73.76096413035727 9.042287670256007 10.275975183626898 66-67 6.442986522142196 71.98887541895458 9.45945945945946 12.108678599443772 68-69 6.938600869999287 65.36761035441774 10.832204235898168 16.861584539684806 70-71 8.453968480353705 58.753476431576686 12.283391570990515 20.509163517079084 72-73 9.413107038436854 49.53291021892605 14.775725593667547 26.27825714896955 74-75 10.632532268416174 48.4275832560793 14.337160379376737 26.60272409612779 76 9.833844398488198 50.2959423803751 13.463595521643015 26.40661769949369 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 1.0 15 0.5 16 1.0 17 2.0 18 1.5 19 1.5 20 3.5 21 5.0 22 6.5 23 9.5 24 9.5 25 8.0 26 17.0 27 28.5 28 30.0 29 29.0 30 45.0 31 65.5 32 70.0 33 70.0 34 81.0 35 107.5 36 141.5 37 160.0 38 188.5 39 223.0 40 301.5 41 515.0 42 656.0 43 1156.0 44 2088.0 45 2485.5 46 2451.0 47 2166.0 48 1511.0 49 918.5 50 696.0 51 559.0 52 338.5 53 228.0 54 201.0 55 172.5 56 124.5 57 107.0 58 109.0 59 96.0 60 71.0 61 52.5 62 46.0 63 42.0 64 26.5 65 13.0 66 9.5 67 8.0 68 5.0 69 1.0 70 0.5 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 14023.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.14283676816658 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37693568956094 31.0703843685374 2 12.698123519766808 9.94081152392498 3 3.935143013299326 4.620979818869001 4 1.275277828384041 1.9967196748199387 5 0.6194206595008198 1.2122940882835342 6 0.45545636728001454 1.0696712543678244 7 0.25505556567680815 0.6988518861869786 8 0.07287301876480233 0.22819653426513586 9 0.12752778283840407 0.4492619268344862 >10 0.8926944798688284 6.902945161520359 >50 0.16396429222080525 4.635242102260572 >100 0.07287301876480233 5.769093631890466 >500 0.018218254691200583 4.485488126649076 >1k 0.036436509382401165 26.920059901590243 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT 1956 13.94851315695643 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT 1819 12.971546744633816 TruSeq Adapter, Index 8 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 629 4.485488126649076 No Hit CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 325 2.317621051130286 No Hit TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 192 1.3691792055908152 No Hit TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT 162 1.1552449547172503 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT 130 0.9270484204521143 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 93 0.6631961777080511 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 86 0.6132781858375526 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT 80 0.5704913356628396 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT 75 0.5348356271839122 No Hit CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 70 0.4991799187049847 No Hit CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 68 0.4849176353134137 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT 61 0.43499964344291525 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 59 0.4207373600513442 No Hit TTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 58 0.4136062183555588 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT 50 0.3565570847892747 No Hit CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 50 0.3565570847892747 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTCTT 39 0.2781145261356343 No Hit CTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 34 0.24245881765670685 No Hit CTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT 34 0.24245881765670685 No Hit CTTATCACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 33 0.23532767596092133 No Hit CTTATTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 30 0.21393425087356485 No Hit CTTATACACATCTCCGAGCCCACGCGACGGCGTCGAATCTCGTATGCCGT 28 0.19967196748199387 No Hit CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTC 28 0.19967196748199387 No Hit CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTC 27 0.19254082578620837 No Hit CTTATACACATCTCCGAGCACACGAGACGGCGTCGAATCTCGTATGCCGT 24 0.1711474006988519 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGACTCTCGTATGCCGTCTT 24 0.1711474006988519 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTAGAATCTCGTATGCCGT 24 0.1711474006988519 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTAGTATGCCGT 23 0.1640162590030664 No Hit TCTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT 23 0.1640162590030664 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCCT 22 0.15688511730728089 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTCTGCCGTCTT 22 0.15688511730728089 No Hit CTTATACACATCTCCGAGCCCACGAGCCGGCGTCGAATCTCGTATGCCGT 22 0.15688511730728089 No Hit ATACACATCTCCGAGCCCACGAGAAGGCGTCGAATCTCGTATGCCGTCTT 20 0.1426228339157099 RNA PCR Primer, Index 13 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCG 19 0.1354916922199244 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGCATCTCGTATGCCGT 19 0.1354916922199244 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGCATCTCGTATGCCGTCTT 19 0.1354916922199244 TruSeq Adapter, Index 18 (95% over 21bp) CTTATATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 19 0.1354916922199244 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCACGTATGCCGT 18 0.12836055052413892 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCAGTCTT 18 0.12836055052413892 No Hit CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTC 17 0.12122940882835342 No Hit ACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCT 17 0.12122940882835342 RNA PCR Primer, Index 8 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACGGCGTAGAATCTCGTATGCCGTCTT 16 0.11409826713256793 TruSeq Adapter, Index 8 (95% over 21bp) ATACACATCTACGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT 16 0.11409826713256793 RNA PCR Primer, Index 13 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCG 15 0.10696712543678243 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.007131141695785495 0.0 6 0.0 0.0 0.0 0.007131141695785495 0.0 7 0.0 0.0 0.0 0.007131141695785495 0.0 8 0.0 0.0 0.0 0.007131141695785495 0.0 9 0.0 0.0 0.0 0.01426228339157099 0.0 10 0.0 0.0 0.0 0.01426228339157099 0.0 11 0.0 0.0 0.0 0.01426228339157099 0.0 12 0.0 0.0 0.0 0.01426228339157099 0.0 13 0.0 0.0 0.0 0.021393425087356487 0.0 14 0.0 0.0 0.0 0.035655708478927475 0.0 15 0.0 0.0 0.0 0.035655708478927475 0.0 16 0.0 0.0 0.0 0.042786850174712975 0.0 17 0.0 0.0 0.0 0.042786850174712975 0.0 18 0.0 0.0 0.0 0.06418027526206946 0.0 19 0.0 0.0 0.0 0.08557370034942595 0.0 20 0.0 0.0 0.0 0.08557370034942595 0.0 21 0.0 0.0 0.0 0.09983598374099693 0.0 22 0.0 0.0 0.0 0.09983598374099693 0.0 23 0.0 0.0 0.0 0.10696712543678243 0.0 24 0.0 0.0 0.0 0.10696712543678243 0.0 25 0.0 0.0 0.0 0.11409826713256792 0.0 26 0.0 0.0 0.0 0.11409826713256792 0.0 27 0.0 0.0 0.0 0.12122940882835342 0.0 28 0.0 0.0 0.0 0.1354916922199244 0.0 29 0.0 0.0 0.0 0.15688511730728089 0.0 30 0.0 0.0 0.0 0.1782785423946374 0.0 31 0.0 0.0 0.0 0.19967196748199387 0.0 32 0.0 0.0 0.0 0.20680310917777936 0.0 33 0.0 0.0 0.0 0.21393425087356485 0.0 34 0.0 0.0 0.0 0.23532767596092136 0.0 35 0.0 0.0 0.0 0.3066390929187763 0.0 36 0.0 0.0 0.0 0.39934393496398773 0.0 37 0.0 0.0 0.0 0.5776224773586252 0.0 38 0.0 0.0 0.0 0.7701633031448335 0.0 39 0.0 0.0 0.0 0.9912286957141838 0.0 40 0.0 0.0 0.0 1.2336875133708907 0.0 41 0.0 0.0 0.0 1.454752905940241 0.0 42 0.0 0.0 0.0 1.640162590030664 0.0 43 0.0 0.0 0.0 2.0751622334735793 0.0 44 0.0 0.0 0.0 2.3889324680881407 0.0 45 0.0 0.0 0.0 2.674178135919561 0.0 46 0.0 0.0 0.0 3.02360407901305 0.0 47 0.0 0.0 0.0 3.280325180061328 0.0 48 0.0 0.0 0.0 3.6939313984168867 0.0 49 0.0 0.0 0.0 3.9934393496398775 0.0 50 0.0 0.0 0.0 4.235898167296584 0.0 51 0.0 0.0 0.0 4.414176709691222 0.0 52 0.0 0.0 0.0 4.6566355273479285 0.0 53 0.0 0.0 0.0 4.949012336875134 0.0 54 0.0 0.0 0.0 5.234258004706554 0.0 55 0.0 0.0 0.0 5.455323397275904 0.0 56 0.0 0.0 0.0 5.6050773728874 0.0 57 0.0 0.0 0.0 5.811880482065178 0.0 58 0.0 0.0 0.0 5.975896741068245 0.0 59 0.0 0.0 0.0 6.161306425158668 0.0 60 0.0 0.0 0.0 6.425158667902731 0.0 61 0.0 0.0 0.0 6.731797760821507 0.0 62 0.0 0.0 0.0 7.024174570348713 0.0 63 0.0 0.0 0.0 7.238108821222277 0.0 64 0.0 0.0 0.0 7.459174213791628 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGAC 15 0.0021791556 70.00001 28 TCTTTAT 15 0.0021791556 70.00001 1 CTTTACA 90 0.0 66.11111 1 TTACACA 90 0.0 66.11111 3 TTTACAC 90 0.0 66.11111 2 CTATACA 80 0.0 65.625 1 TTTATAC 25 2.310114E-4 56.000004 3 CTTATAC 435 0.0 43.448273 1 CTTAATA 35 0.0012137684 40.0 1 TAATACA 35 0.0012137684 40.0 3 AATACAC 35 0.0012137684 40.0 4 TTAATAC 35 0.0012137684 40.0 2 TTATACA 475 0.0 39.05263 2 TATACAC 565 0.0 32.83186 3 TCGAATA 50 0.0069734883 28.000002 30 CGAATAT 50 0.0069734883 28.000002 31 AAAACCA 50 0.0069734883 28.000002 67 AAAAACC 80 8.085939E-5 26.25 66 TCGAATC 740 0.0 23.64865 33 CGAATCT 730 0.0 23.49315 34 >>END_MODULE